MD07G1088200.v1.1

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006723
Physical Location & Seq
Reverse (-)
9222808 .. 9225397
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1088200.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGCCATGCCAGATAGCCAGGAATCGATTTTACCCAGAGATGGAACTGTGATTGAGCAAACACTATTTGTTAAACTTGGTCGTTGGCAGTGGTGGTTTTTGGTGGTACTCAACGTCGTCTTCCTCCTTGTGGGGCAGTCTGCTGCAGTTTTACTGGGAAGATTCTATTATGACCAGGGTGGCAATAGTAAGTGGTTGGCTACTCTTGTCCAAACTGCAGCCTTCCCAGTCCTTTTCATCCCGCTTTATTTTCTTCCTTCATCCAAAGAACAAGCAACTTCTTCAAACCCGCCTTCCATTAAAGTTCTTGCCTTGGTTTACTTCTCTATTGGAGTGCTCTTAGCTGGTGACAACATGTTGTATTCTGTCGGACTCTTGTACCTATCTGCCTCCACTTATTCCCTCATATGCGCAACTCAGTTAGCTTTTAATGCAGTTTTCTCGTACTTCCTCAACTCTCAGAAGTTCACAATGCTAATTCTTAATTCAGTGATTATCGTGTCATTCTCAGCAGCTCTTATTGCCGTCGATGATGATTCTGGAGGACCAACAGGAGTCTCAAAGGGGAAATATATCCTTGGCTTCATCTGTACCATTGGAGCCTCTGCACTGTACTCTCTTTTGCTTTCCCTCATGCAGCTTTCTTTCCAAAAGGTTTTAAAAAAGGAAACGTTTTCTGTGGTTCTGGAGATGCAAATCTATACGGCCCTGGTCGCTACTTGTGCTGCAATTGTAGGTCTTTTTGCAAGTGGGGAATGGAGGAGTTTGAATGGGGAAATGGAAAACTATGGCAAGGGACGAGTTTCTTACGTGATGACCCTAGTTTGGACAGCTGTGGCTTGGCAAATTTGTTCTGTTGGTGTCGTGGGCTTAATTTTCGTGGTTTCTTCTCTCTTCTCCAATGTAATCAGTACTCTCTCTTTGGCTGTTACTCCTATAGCCGCTGTGATAATCTTCCATGACAAGATGAACGGTGCCAAGGTAATTGCTATGCTTCTGGCTCTTTGGGGTTTCGCCTCTTATATTTATCAGAACTATCTCGATGACTCTAAGGCAAGAAGAAGACTAACTGATGCTAGGGAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

363

Amino Acids

39.59

Weight (kDa)

8.54

Isoelectric Point (pI)

27.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 34 - 351 3.6e-131 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 974
AciI CCGC 3 cut(s) 242, 290, 942
AclI AACGTT 1 cut(s) 671
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 846
AfaI GTAC 6 cut(s) 108, 380, 446, 592, 614, 913
AfiI CCNNNNNNNGG 3 cut(s) 41, 130, 825
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 2 cut(s) 285, 769
AjnI CCWGG 3 cut(s) 18, 174, 708
AluBI AGCT 5 cut(s) 344, 425, 515, 640, 833
AluI AGCT 5 cut(s) 344, 425, 515, 640, 833
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 562
AoxI GGCC 2 cut(s) 3, 705
ApeKI GCWGC 5 cut(s) 143, 218, 512, 637, 725
ApoI RAATTY 1 cut(s) 846
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 2 cut(s) 545, 706
AsuHPI GGTGA 1 cut(s) 359
AvaII GGWCC 1 cut(s) 545
BaeI ACNNNNGTAYC 2 cut(s) 362, 395
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 974
BbsI GAAGAC 2 cut(s) 112, 1069
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 5 cut(s) 130, 230, 524, 649, 712
BccI CCATC 1 cut(s) 35
BceAI ACGGC 2 cut(s) 509, 720
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BciT130I CCWGG 3 cut(s) 20, 176, 710
BcoDI GTCTC 1 cut(s) 562
BfaI CTAG 2 cut(s) 821, 1077
BfmI CTRYAG 3 cut(s) 144, 216, 936
BisI GCNGC 6 cut(s) 144, 219, 513, 638, 726, 942
BlsI GCNGC 6 cut(s) 145, 220, 514, 639, 727, 943
BmcAI AGTACT 1 cut(s) 913
Bme1390I CCNGG 3 cut(s) 20, 176, 710
Bme18I GGWCC 1 cut(s) 545
BmgT120I GGNCC 2 cut(s) 545, 706
BmiI GGNNCC 3 cut(s) 601, 976, 1083
BmrFI CCNGG 3 cut(s) 20, 176, 710
BmrI ACTGGG 2 cut(s) 164, 221
BmsI GCATC 2 cut(s) 681, 1063
BmuI ACTGGG 2 cut(s) 164, 221
BpiI GAAGAC 2 cut(s) 112, 1069
BpmI CTGGAG 2 cut(s) 561, 707
Bsa29I ATCGAT 1 cut(s) 26
BsaAI YACGTR 1 cut(s) 811
BsaBI GATNNNNATC 1 cut(s) 695
BsaJI CCNNGG 5 cut(s) 175, 312, 577, 708, 978
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 130, 825
Bse1I ACTGG 2 cut(s) 159, 227
Bse8I GATNNNNATC 1 cut(s) 695
BseBI CCWGG 3 cut(s) 20, 176, 710
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 5 cut(s) 175, 312, 577, 708, 978
BseGI GGATG 2 cut(s) 237, 260
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 3 cut(s) 41, 130, 825
BseMII CTCAG 3 cut(s) 431, 473, 522
BseNI ACTGG 2 cut(s) 159, 227
BseRI GAGGAG 1 cut(s) 775
BseXI GCAGC 5 cut(s) 130, 230, 524, 649, 712
BsgI GTGCAG 1 cut(s) 591
BshFI GGCC 2 cut(s) 5, 707
BshNI GGYRCC 1 cut(s) 974
BshVI ATCGAT 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 339
BslFI GGGAC 1 cut(s) 810
BslI CCNNNNNNNGG 3 cut(s) 41, 130, 825
BsmAI GTCTC 1 cut(s) 562
BsmFI GGGAC 1 cut(s) 810
BsnI GGCC 2 cut(s) 5, 707
Bsp1286I GDGCHC 1 cut(s) 339
BspACI CCGC 3 cut(s) 242, 290, 942
BspANI GGCC 2 cut(s) 5, 707
BspCNI CTCAG 3 cut(s) 430, 472, 521
BspDI ATCGAT 1 cut(s) 26
BspLI GGNNCC 3 cut(s) 601, 976, 1083
BspMAI CTGCAG 2 cut(s) 148, 220
BspT107I GGYRCC 1 cut(s) 974
BsrI ACTGG 2 cut(s) 159, 227
BssECI CCNNGG 5 cut(s) 175, 312, 577, 708, 978
BssT1I CCWWGG 3 cut(s) 312, 577, 978
Bst2UI CCWGG 3 cut(s) 20, 176, 710
Bst4CI ACNGT 3 cut(s) 49, 612, 974
Bst6I CTCTTC 1 cut(s) 899
BstBAI YACGTR 1 cut(s) 811
BstDEI CTNAG 5 cut(s) 340, 417, 459, 508, 1050
BstF5I GGATG 2 cut(s) 237, 260
BstHHI GCGC 1 cut(s) 413
BstMAI GTCTC 1 cut(s) 562
BstMWI GCNNNNNNNGC 4 cut(s) 431, 521, 713, 722
BstNI CCWGG 3 cut(s) 20, 176, 710
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 3 cut(s) 18, 174, 708
BstSFI CTRYAG 3 cut(s) 144, 216, 936
BstV1I GCAGC 5 cut(s) 130, 230, 524, 649, 712
BstV2I GAAGAC 2 cut(s) 112, 1069
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 2 cut(s) 5, 707
BsuTUI ATCGAT 1 cut(s) 26
BtsCI GGATG 2 cut(s) 237, 260
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 3 cut(s) 95, 495, 608
CfoI GCGC 1 cut(s) 413
Cfr13I GGNCC 2 cut(s) 545, 706
ClaI ATCGAT 1 cut(s) 26
Csp6I GTAC 6 cut(s) 107, 379, 445, 591, 613, 912
CviAII CATG 4 cut(s) 7, 355, 634, 959
CviQI GTAC 6 cut(s) 107, 379, 445, 591, 613, 912
DdeI CTNAG 5 cut(s) 340, 417, 459, 508, 1050
DraI TTTAAA 1 cut(s) 660
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 899
EarI CTCTTC 1 cut(s) 899
Eco130I CCWWGG 3 cut(s) 312, 577, 978
Eco47I GGWCC 1 cut(s) 545
EcoRII CCWGG 3 cut(s) 18, 174, 708
EcoT14I CCWWGG 3 cut(s) 312, 577, 978
ErhI CCWWGG 3 cut(s) 312, 577, 978
FaeI CATG 4 cut(s) 10, 358, 637, 962
FaqI GGGAC 1 cut(s) 810
FatI CATG 4 cut(s) 6, 354, 633, 958
FauI CCCGC 2 cut(s) 249, 297
FauNDI CATATG 1 cut(s) 407
Fnu4HI GCNGC 6 cut(s) 144, 219, 513, 638, 726, 942
FokI GGATG 2 cut(s) 224, 247
Fsp4HI GCNGC 6 cut(s) 144, 219, 513, 638, 726, 942
FspBI CTAG 2 cut(s) 821, 1077
FspI TGCGCA 1 cut(s) 412
GlaI GCGC 1 cut(s) 412
GluI GCNGC 6 cut(s) 144, 219, 513, 638, 726, 942
GsuI CTGGAG 2 cut(s) 561, 707
HaeIII GGCC 2 cut(s) 5, 707
HhaI GCGC 1 cut(s) 413
Hin1II CATG 4 cut(s) 10, 358, 637, 962
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HinfI GANTC 6 cut(s) 23, 162, 372, 536, 555, 1046
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 2 cut(s) 319, 468
Hpy188I TCNGA 3 cut(s) 371, 462, 1032
Hpy188III TCNNGA 3 cut(s) 540, 686, 1040
Hpy8I GTNNAC 2 cut(s) 319, 468
Hpy99I CGWCG 2 cut(s) 119, 530
HpyAV CCTTC 3 cut(s) 232, 267, 303
HpyCH4III ACNGT 3 cut(s) 49, 612, 974
HpyCH4IV ACGT 3 cut(s) 114, 671, 810
HpyCH4V TGCA 8 cut(s) 146, 218, 434, 608, 637, 694, 728, 746
HpyF10VI GCNNNNNNNGC 4 cut(s) 431, 521, 713, 722
HpyF3I CTNAG 5 cut(s) 340, 417, 459, 508, 1050
HpySE526I ACGT 3 cut(s) 114, 671, 810
Hsp92II CATG 4 cut(s) 10, 358, 637, 962
HspAI GCGC 1 cut(s) 411
LmnI GCTCC 1 cut(s) 599
Lsp1109I GCAGC 5 cut(s) 130, 230, 524, 649, 712
LweI GCATC 2 cut(s) 681, 1063
MaeI CTAG 2 cut(s) 821, 1077
MaeII ACGT 3 cut(s) 114, 671, 810
MaeIII GTNAC 2 cut(s) 347, 928
MboII GAAGA 9 cut(s) 112, 171, 245, 273, 879, 886, 946, 1071, 1074
MfeI CAATTG 1 cut(s) 729
MhlI GDGCHC 1 cut(s) 339
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 477, 484, 729, 846, 873, 984
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 366, 564, 1040
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 9 cut(s) 134, 400, 413, 461, 536, 613, 641, 753, 1027
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 72, 300, 429, 483, 659, 872
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 833, 944
MspR9I CCNGG 3 cut(s) 20, 176, 710
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 3 cut(s) 20, 176, 710
MwoI GCNNNNNNNGC 4 cut(s) 431, 521, 713, 722
NdeI CATATG 1 cut(s) 407
NlaIII CATG 4 cut(s) 10, 358, 637, 962
NlaIV GGNNCC 3 cut(s) 601, 976, 1083
NmuCI GTSAC 1 cut(s) 347
NsbI TGCGCA 1 cut(s) 412
NspI RCATGY 1 cut(s) 358
PciI ACATGT 1 cut(s) 354
PfeI GAWTC 3 cut(s) 23, 162, 536
PkrI GCNGC 6 cut(s) 145, 220, 514, 639, 727, 943
PleI GAGTC 3 cut(s) 366, 563, 1040
PpsI GAGTC 3 cut(s) 366, 563, 1040
Ppu21I YACGTR 1 cut(s) 811
PscI ACATGT 1 cut(s) 354
Psp1406I AACGTT 1 cut(s) 671
Psp6I CCWGG 3 cut(s) 18, 174, 708
PspGI CCWGG 3 cut(s) 18, 174, 708
PspN4I GGNNCC 3 cut(s) 601, 976, 1083
PspPI GGNCC 2 cut(s) 545, 706
PstI CTGCAG 2 cut(s) 148, 220
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 6 cut(s) 108, 380, 446, 592, 614, 913
RsaNI GTAC 6 cut(s) 107, 379, 445, 591, 613, 912
SaqAI TTAA 6 cut(s) 72, 300, 429, 483, 659, 872
SatI GCNGC 6 cut(s) 144, 219, 513, 638, 726, 942
Sau96I GGNCC 2 cut(s) 545, 706
ScaI AGTACT 1 cut(s) 913
SchI GAGTC 3 cut(s) 366, 564, 1040
ScrFI CCNGG 3 cut(s) 20, 176, 710
SduI GDGCHC 1 cut(s) 339
SfaNI GCATC 2 cut(s) 681, 1063
SfcI CTRYAG 3 cut(s) 144, 216, 936
SinI GGWCC 1 cut(s) 545
Sse9I AATT 6 cut(s) 477, 484, 729, 846, 873, 984
SsiI CCGC 3 cut(s) 242, 290, 942
SspMI CTAG 2 cut(s) 821, 1077
StyD4I CCNGG 3 cut(s) 18, 174, 708
StyI CCWWGG 3 cut(s) 312, 577, 978
TaaI ACNGT 3 cut(s) 49, 612, 974
TaiI ACGT 3 cut(s) 117, 674, 813
TaqI TCGA 3 cut(s) 26, 528, 1041
TasI AATT 6 cut(s) 477, 484, 729, 846, 873, 984
TatI WGTACW 2 cut(s) 612, 911
TauI GCSGC 1 cut(s) 944
TfiI GAWTC 3 cut(s) 23, 162, 536
Tru1I TTAA 6 cut(s) 72, 300, 429, 483, 659, 872
Tru9I TTAA 6 cut(s) 72, 300, 429, 483, 659, 872
TscAI CASTG 3 cut(s) 95, 495, 615
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 5 cut(s) 143, 218, 512, 637, 725
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 4 cut(s) 226, 249, 574, 983
TspRI CASTG 3 cut(s) 95, 495, 615
VpaK11BI GGWCC 1 cut(s) 545
XapI RAATTY 1 cut(s) 846
XceI RCATGY 1 cut(s) 358
XspI CTAG 2 cut(s) 821, 1077
ZrmI AGTACT 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.