Rorug01G0170200

retrograde vesicle-mediated transport, Golgi to ER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006723
Physical Location & Seq
Forward (+)
23136577 .. 23140602
4026 bp
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UTR
Exon/CDS
Intron
Rorug01G0170200.1

Sequence Viewer

Length: 555 bp
ATGGATACAGATGAAGATGCAGCGGAGGATGTCATGGAAGCAGAAGACGTGGTGGCAGGTATATTCCTCTCAGTTTTCCAACGGGATCCAGAGACTCGTTTGGCGGGTTATAGAGTGCTGACTGGGTTGGCGGCTCGCCCATGGTGCCTCATGGAGATTTGCTCAAAGCAAGAGATAATAAATATAGTGACTGATGCCGCTACTGAAACCACAAAACTAGGCATGGAAGCAAGATATCATTGTTGCAAGGCTATCCATATGGCTTTTAACATGTCAAATAAACTTTCAAGCGACCCAGCGCTTGGTGGGATTGCAGCAAAGGTTTTGGAAGCTTTAGACGTGCTCAAGAAGAAACCAGATGCTCAGGCTGAAGAAGTTCTTTCAATTGAGAAGCTTATCGGCGAGGCATACTCCATCATTGACAAAGCAGTGAAGGTTGGAACATTGCATAGGAATACTGGAGCACGCAGAAAGTCTCGATTGGCCAGAAGAAAGAAGGCTGTAGAGATCCAAAAAGGTTGGTATACCCCGGCTGATTCTGAAGTTACTGCCTGA

Protein Analysis

184

Amino Acids

20.23

Weight (kDa)

5.98

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S20p PF01649 121 - 164 3.4e-10 Ribosomal protein S20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 302
AccI GTMKAC 1 cut(s) 524
AciI CCGC 4 cut(s) 23, 104, 131, 198
AclWI GGATC 3 cut(s) 80, 93, 502
AcoI YGGCCR 1 cut(s) 483
AcuI CTGAAG 1 cut(s) 390
AfeI AGCGCT 1 cut(s) 300
AfiI CCNNNNNNNGG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 2 cut(s) 288, 384
AjiI CACGTC 2 cut(s) 49, 340
AluBI AGCT 2 cut(s) 332, 394
AluI AGCT 2 cut(s) 332, 394
Alw21I GWGCWC 2 cut(s) 345, 466
Alw26I GTCTC 2 cut(s) 86, 480
AlwI GGATC 3 cut(s) 80, 93, 502
Aor51HI AGCGCT 1 cut(s) 300
AoxI GGCC 1 cut(s) 483
ApeKI GCWGC 2 cut(s) 20, 314
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 301
AsuC2I CCSGG 1 cut(s) 530
BalI TGGCCA 1 cut(s) 485
BamHI GGATCC 1 cut(s) 85
BanI GGYRCC 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 345, 466
BbvI GCAGC 2 cut(s) 32, 326
BccI CCATC 1 cut(s) 422
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 2 cut(s) 86, 480
BfaI CTAG 1 cut(s) 218
BfmI CTRYAG 1 cut(s) 501
BfoI RGCGCY 1 cut(s) 302
BfuAI ACCTGC 1 cut(s) 47
BisI GCNGC 4 cut(s) 21, 132, 198, 315
BlsI GCNGC 4 cut(s) 22, 133, 199, 316
Bme1390I CCNGG 1 cut(s) 530
BmgBI CACGTC 2 cut(s) 49, 340
BmiI GGNNCC 2 cut(s) 87, 146
BmrFI CCNGG 1 cut(s) 530
BmrI ACTGGG 1 cut(s) 132
BmsI GCATC 3 cut(s) 7, 184, 349
BmuI ACTGGG 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 4 cut(s) 146, 178, 395, 427
BpmI CTGGAG 1 cut(s) 480
Bpu10I CCTNAGC 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 530
BsaJI CCNNGG 2 cut(s) 140, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse1I ACTGG 2 cut(s) 127, 463
Bse3DI GCAATG 1 cut(s) 443
BseDI CCNNGG 2 cut(s) 140, 528
BseGI GGATG 1 cut(s) 34
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMI GCAATG 1 cut(s) 443
BseMII CTCAG 2 cut(s) 84, 377
BseNI ACTGG 2 cut(s) 127, 463
BseXI GCAGC 2 cut(s) 32, 326
BseYI CCCAGC 1 cut(s) 295
BshFI GGCC 1 cut(s) 485
BshNI GGYRCC 1 cut(s) 144
BsiHKAI GWGCWC 2 cut(s) 345, 466
BsiSI CCGG 1 cut(s) 530
BslI CCNNNNNNNGG 1 cut(s) 302
BsmAI GTCTC 2 cut(s) 86, 480
BsnI GGCC 1 cut(s) 485
Bsp1286I GDGCHC 2 cut(s) 345, 466
Bsp143I GATC 2 cut(s) 85, 507
Bsp19I CCATGG 1 cut(s) 140
BspACI CCGC 4 cut(s) 23, 104, 131, 198
BspANI GGCC 1 cut(s) 485
BspCNI CTCAG 2 cut(s) 83, 376
BspLI GGNNCC 2 cut(s) 87, 146
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 80, 93, 502
BspT107I GGYRCC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 443
BsrI ACTGG 2 cut(s) 127, 463
BssECI CCNNGG 2 cut(s) 140, 528
BssMI GATC 2 cut(s) 85, 507
BssNAI GTATAC 1 cut(s) 525
BssT1I CCWWGG 1 cut(s) 140
Bst1107I GTATAC 1 cut(s) 525
BstC8I GCNNGC 2 cut(s) 136, 466
BstDEI CTNAG 2 cut(s) 70, 363
BstDSI CCRYGG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 34
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 2 cut(s) 88, 510
BstMAI GTCTC 2 cut(s) 86, 480
BstMBI GATC 2 cut(s) 85, 507
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 1 cut(s) 528
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 2 cut(s) 32, 326
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 85, 507
BstYI RGATCY 2 cut(s) 85, 507
BstZ17I GTATAC 1 cut(s) 525
BsuRI GGCC 1 cut(s) 485
BtgI CCRYGG 1 cut(s) 140
BtrI CACGTC 2 cut(s) 49, 340
BtsCI GGATG 1 cut(s) 34
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 435
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 2 cut(s) 136, 466
CfoI GCGC 1 cut(s) 301
CviAII CATG 5 cut(s) 34, 141, 151, 223, 271
CviJI RGCY 9 cut(s) 134, 251, 263, 332, 368, 394, 485, 500, 533
CviKI_1 RGCY 9 cut(s) 134, 251, 263, 332, 368, 394, 485, 500, 533
DdeI CTNAG 2 cut(s) 70, 363
DpnI GATC 2 cut(s) 87, 509
DpnII GATC 2 cut(s) 85, 507
EaeI YGGCCR 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 140
Eco32I GATATC 1 cut(s) 236
Eco47III AGCGCT 1 cut(s) 300
Eco57I CTGAAG 1 cut(s) 390
EcoRV GATATC 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 5 cut(s) 37, 144, 154, 226, 274
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FatI CATG 5 cut(s) 33, 140, 150, 222, 270
FauI CCCGC 1 cut(s) 97
FauNDI CATATG 1 cut(s) 258
FblI GTMKAC 1 cut(s) 524
Fnu4HI GCNGC 4 cut(s) 21, 132, 198, 315
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 4 cut(s) 21, 132, 198, 315
FspBI CTAG 1 cut(s) 218
GlaI GCGC 1 cut(s) 300
GluI GCNGC 4 cut(s) 21, 132, 198, 315
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 1 cut(s) 485
HapII CCGG 1 cut(s) 530
HhaI GCGC 1 cut(s) 301
Hin1II CATG 5 cut(s) 37, 144, 154, 226, 274
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HindIII AAGCTT 2 cut(s) 330, 392
HinfI GANTC 2 cut(s) 94, 536
HpaII CCGG 1 cut(s) 530
Hpy166II GTNNAC 1 cut(s) 525
Hpy188I TCNGA 1 cut(s) 541
Hpy188III TCNNGA 3 cut(s) 89, 346, 477
Hpy8I GTNNAC 1 cut(s) 525
HpyAV CCTTC 2 cut(s) 427, 490
HpyCH4IV ACGT 2 cut(s) 48, 339
HpyCH4V TGCA 4 cut(s) 20, 246, 314, 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 2 cut(s) 70, 363
HpySE526I ACGT 2 cut(s) 48, 339
Hsp92II CATG 5 cut(s) 37, 144, 154, 226, 274
HspAI GCGC 1 cut(s) 299
Kzo9I GATC 2 cut(s) 85, 507
LmnI GCTCC 1 cut(s) 461
LpnPI CCDG 9 cut(s) 42, 102, 108, 309, 350, 369, 444, 499, 543
Lsp1109I GCAGC 2 cut(s) 32, 326
LweI GCATC 3 cut(s) 7, 184, 349
MaeI CTAG 1 cut(s) 218
MaeII ACGT 2 cut(s) 48, 339
MaeIII GTNAC 2 cut(s) 187, 544
MalI GATC 2 cut(s) 87, 509
MboI GATC 2 cut(s) 85, 507
MboII GAAGA 5 cut(s) 26, 56, 361, 383, 501
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 2 cut(s) 85, 507
MhlI GDGCHC 2 cut(s) 345, 466
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 1 cut(s) 384
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 2 cut(s) 103, 418
MnlI CCTC 4 cut(s) 19, 77, 158, 397
Mox20I TGGCCA 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 375
MscI TGGCCA 1 cut(s) 485
MseI TTAA 1 cut(s) 267
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 1 cut(s) 530
MspR9I CCNGG 1 cut(s) 530
MunI CAATTG 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 144
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 1 cut(s) 140
NdeI CATATG 1 cut(s) 258
NdeII GATC 2 cut(s) 85, 507
NlaIII CATG 5 cut(s) 37, 144, 154, 226, 274
NlaIV GGNNCC 2 cut(s) 87, 146
NmuCI GTSAC 1 cut(s) 187
NspI RCATGY 1 cut(s) 274
PciI ACATGT 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 1 cut(s) 536
PflMI CCANNNNNTGG 1 cut(s) 302
PkrI GCNGC 4 cut(s) 22, 133, 199, 316
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PscI ACATGT 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 295
PspN4I GGNNCC 2 cut(s) 87, 146
PsuI RGATCY 2 cut(s) 85, 507
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 4 cut(s) 21, 132, 198, 315
Sau3AI GATC 2 cut(s) 85, 507
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 530
SduI GDGCHC 2 cut(s) 345, 466
SetI ASST 8 cut(s) 51, 61, 324, 334, 342, 396, 438, 520
SfaNI GCATC 3 cut(s) 7, 184, 349
SfcI CTRYAG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 1 cut(s) 384
SsiI CCGC 4 cut(s) 23, 104, 131, 198
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 528
StyI CCWWGG 1 cut(s) 140
TaiI ACGT 2 cut(s) 51, 342
TaqI TCGA 1 cut(s) 478
TasI AATT 1 cut(s) 384
TauI GCSGC 2 cut(s) 134, 200
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 2 cut(s) 20, 314
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 1 cut(s) 27
TspRI CASTG 1 cut(s) 435
Van91I CCANNNNNTGG 1 cut(s) 302
XceI RCATGY 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 524
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.