Rh1DG185500

retrograde vesicle-mediated transport, Golgi to ER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006723
Physical Location & Seq
Reverse (-)
37361415 .. 37364514
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG185500.1

Sequence Viewer

Length: 1107 bp
ATGCCACCACTACCGCCAGATAGCCAAGAAGCCATCCCATTCAAAAATGAAACTGTGATGGACCCAACACTATTGATCAAACTTGGCCGCTGGCAATGGTGGTTTTTGGTGGCAATCAACATATTCTTCCTCATTGTGGGGCAGGCTGGTGCTGTGTTACTCGGAAGATTTTATTATGACCAGGGTGGTAATAGTACGTGGATGGCTACTCTTGTGCAAACCGCAGCTTTTCCAGTTCTTGTTATCCCACTTTATTTTCTTCCTTCACAGAAAGAAGATCAATCAACTTCTTCAACTTCACCTTCCCTTCTAGTTCTTGCCTTGATTTACTTCGTTCTTGGACTGGTCATTGCTGGTGACAACATGTTGTATTCTACTGGACTCTTGTACCTATCTGCCTCTACATACTCCATCATTTCTTCAACTCAATTAGCTTTCAATGCAGTCTTCTCATACTTCATCAATTCTCAGAAGTTCAGTGCATTGATTTTCAATTCGGTGATTATCCTGACATTGTCCGTGGCTCTTATTGGTGTGAATGATGACTCGGATGGACCTGCAGGGGTATCAACGCAGAAACATATCATTGGCTTTATCTGTACCCTTGCTGCTTCTGCAATCTACTCCCTTCTACTCTCCCTCATGCAGCTTACTTTCCAGAAGGTTCTGAAAAAGGAAACTTTCTCTGTGGTTTTGCAAATGCAAATTTTTACAGCACTTGTTGCGACTTGTGCTTCAGTTGTGGGTCTTTTTGCAAGTGGGGATTGGAAGACTTTACATGGAGAAATGGAAAGTTTTGGTAAGGGAAGAGTTTCGTATGTGATGACTTTGGTGTGGACAGCTGTTTCTTGGCAAGTTTGTTCTGTTGGTGTGGTGGGTTTAATTTTCGTGGTGTCTTCCCTATTCTCGAACGTAATCAGTACTCTCTCTTTGGCTGTTACTCCTATAGCTGCTGTGATAGTTTTCCATGACAAGATGAATGGTGTGAAGATAATTGCTATGCTAATGGCTATATGGGGTTCTGCTTCGTATATTTATCAAAACTACCTTGATGATGCCAAGGCAAGAAGAGCAGTGGCTGATGTTAGTGAATCGCACATTGCGTGA

Protein Analysis

368

Amino Acids

40.01

Weight (kDa)

6.06

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 36 - 353 1.4e-131 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AciI CCGC 3 cut(s) 14, 88, 222
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 1 cut(s) 705
AcuI CTGAAG 1 cut(s) 720
AfaI GTAC 4 cut(s) 196, 389, 601, 922
AfiI CCNNNNNNNGG 1 cut(s) 136
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 5 cut(s) 43, 294, 423, 439, 493
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 5 cut(s) 227, 434, 649, 842, 950
AluI AGCT 5 cut(s) 227, 434, 649, 842, 950
AlwNI CAGNNNCTG 1 cut(s) 1079
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 4 cut(s) 224, 608, 646, 950
ApoI RAATTY 1 cut(s) 705
Asp700I GAANNNNTTC 1 cut(s) 811
AspS9I GGNCC 2 cut(s) 61, 554
AsuHPI GGTGA 3 cut(s) 291, 368, 511
AvaII GGWCC 2 cut(s) 61, 554
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BbsI GAAGAC 3 cut(s) 439, 776, 888
BbvI GCAGC 4 cut(s) 236, 595, 658, 937
BccI CCATC 5 cut(s) 41, 52, 196, 419, 545
BciT130I CCWGG 1 cut(s) 182
BclI TGATCA 1 cut(s) 75
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 2 cut(s) 558, 945
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 5 cut(s) 88, 225, 609, 647, 951
BlsI GCNGC 5 cut(s) 89, 226, 610, 648, 952
BmcAI AGTACT 1 cut(s) 922
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 2 cut(s) 61, 554
BmgT120I GGNCC 2 cut(s) 61, 554
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 1 cut(s) 1045
BpiI GAAGAC 3 cut(s) 439, 776, 888
BsaAI YACGTR 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 181, 519, 1059
Bsc4I CCNNNNNNNGG 1 cut(s) 136
Bse1I ACTGG 3 cut(s) 233, 348, 382
Bse3DI GCAATG 3 cut(s) 101, 348, 1098
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 3 cut(s) 181, 519, 1059
BseGI GGATG 3 cut(s) 33, 207, 556
BseLI CCNNNNNNNGG 1 cut(s) 136
BseMI GCAATG 3 cut(s) 101, 348, 1098
BseMII CTCAG 1 cut(s) 482
BseNI ACTGG 3 cut(s) 233, 348, 382
BseXI GCAGC 4 cut(s) 236, 595, 658, 937
BshFI GGCC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 136
BsnI GGCC 1 cut(s) 87
Bsp143I GATC 2 cut(s) 75, 277
BspACI CCGC 3 cut(s) 14, 88, 222
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 562
BspMI ACCTGC 1 cut(s) 565
BspQI GCTCTTC 1 cut(s) 1063
BsrDI GCAATG 3 cut(s) 101, 348, 1098
BsrI ACTGG 3 cut(s) 233, 348, 382
BssECI CCNNGG 3 cut(s) 181, 519, 1059
BssMI GATC 2 cut(s) 75, 277
BssT1I CCWWGG 1 cut(s) 1059
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 2 cut(s) 802, 1063
BstAPI GCANNNNNTGC 1 cut(s) 722
BstBAI YACGTR 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 92, 144
BstDEI CTNAG 1 cut(s) 468
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 3 cut(s) 33, 207, 556
BstKTI GATC 2 cut(s) 78, 280
BstMBI GATC 2 cut(s) 75, 277
BstMWI GCNNNNNNNGC 5 cut(s) 440, 614, 722, 731, 1070
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 2 cut(s) 558, 945
BstV1I GCAGC 4 cut(s) 236, 595, 658, 937
BstV2I GAAGAC 3 cut(s) 439, 776, 888
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 3 cut(s) 33, 207, 556
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 2 cut(s) 484, 1080
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 92, 144
CaiI CAGNNNCTG 1 cut(s) 1079
Cfr13I GGNCC 2 cut(s) 61, 554
Csp6I GTAC 4 cut(s) 195, 388, 600, 921
CviAII CATG 4 cut(s) 364, 643, 779, 968
CviQI GTAC 4 cut(s) 195, 388, 600, 921
DdeI CTNAG 1 cut(s) 468
DpnI GATC 2 cut(s) 77, 279
DpnII GATC 2 cut(s) 75, 277
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 2 cut(s) 802, 1063
EarI CTCTTC 2 cut(s) 802, 1063
Eco130I CCWWGG 1 cut(s) 1059
Eco47I GGWCC 2 cut(s) 61, 554
Eco57I CTGAAG 1 cut(s) 720
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 1059
ErhI CCWWGG 1 cut(s) 1059
FaeI CATG 4 cut(s) 367, 646, 782, 971
FatI CATG 4 cut(s) 363, 642, 778, 967
FbaI TGATCA 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 88, 225, 609, 647, 951
FokI GGATG 3 cut(s) 20, 214, 563
Fsp4HI GCNGC 5 cut(s) 88, 225, 609, 647, 951
FspBI CTAG 1 cut(s) 311
GluI GCNGC 5 cut(s) 88, 225, 609, 647, 951
HaeIII GGCC 1 cut(s) 87
Hin1II CATG 4 cut(s) 367, 646, 782, 971
HinfI GANTC 3 cut(s) 381, 545, 1091
HphI GGTGA 3 cut(s) 291, 368, 511
Hpy166II GTNNAC 1 cut(s) 837
Hpy188I TCNGA 4 cut(s) 164, 471, 550, 669
Hpy188III TCNNGA 3 cut(s) 508, 658, 907
Hpy8I GTNNAC 1 cut(s) 837
HpyAV CCTTC 5 cut(s) 273, 312, 317, 638, 655
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 2 cut(s) 197, 912
HpyCH4V TGCA 9 cut(s) 217, 443, 482, 560, 617, 646, 697, 703, 755
HpyF10VI GCNNNNNNNGC 5 cut(s) 440, 614, 722, 731, 1070
HpyF3I CTNAG 1 cut(s) 468
HpySE526I ACGT 2 cut(s) 197, 912
Hsp92II CATG 4 cut(s) 367, 646, 782, 971
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 2 cut(s) 75, 277
LguI GCTCTTC 1 cut(s) 1063
Lsp1109I GCAGC 4 cut(s) 236, 595, 658, 937
LweI GCATC 1 cut(s) 1045
MaeI CTAG 1 cut(s) 311
MaeII ACGT 2 cut(s) 197, 912
MaeIII GTNAC 3 cut(s) 156, 356, 937
MalI GATC 2 cut(s) 77, 279
MboI GATC 2 cut(s) 75, 277
MluCI AATT 6 cut(s) 428, 463, 493, 705, 882, 993
MlyI GAGTC 2 cut(s) 375, 539
MnlI CCTC 3 cut(s) 140, 409, 650
MroXI GAANNNNTTC 1 cut(s) 811
MseI TTAA 1 cut(s) 881
MspA1I CMGCKG 2 cut(s) 90, 842
MspR9I CCNGG 1 cut(s) 182
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 5 cut(s) 440, 614, 722, 731, 1070
NdeII GATC 2 cut(s) 75, 277
NlaIII CATG 4 cut(s) 367, 646, 782, 971
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 356
NspI RCATGY 1 cut(s) 367
PciI ACATGT 1 cut(s) 363
PciSI GCTCTTC 1 cut(s) 1063
PcsI WCGNNNNNNNCGW 1 cut(s) 1100
PdmI GAANNNNTTC 1 cut(s) 811
PfeI GAWTC 1 cut(s) 1091
PflFI GACNNNGTC 1 cut(s) 514
PkrI GCNGC 5 cut(s) 89, 226, 610, 648, 952
PleI GAGTC 2 cut(s) 375, 539
PpsI GAGTC 2 cut(s) 375, 539
Ppu21I YACGTR 1 cut(s) 198
PscI ACATGT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 2 cut(s) 61, 554
PstI CTGCAG 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 1079
PsyI GACNNNGTC 1 cut(s) 514
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 4 cut(s) 196, 389, 601, 922
RsaNI GTAC 4 cut(s) 195, 388, 600, 921
SapI GCTCTTC 1 cut(s) 1063
SaqAI TTAA 1 cut(s) 881
SatI GCNGC 5 cut(s) 88, 225, 609, 647, 951
Sau3AI GATC 2 cut(s) 75, 277
Sau96I GGNCC 2 cut(s) 61, 554
SbfI CCTGCAGG 1 cut(s) 562
ScaI AGTACT 1 cut(s) 922
SchI GAGTC 2 cut(s) 375, 539
ScrFI CCNGG 1 cut(s) 182
SdaI CCTGCAGG 1 cut(s) 562
SfaNI GCATC 1 cut(s) 1045
SfcI CTRYAG 2 cut(s) 558, 945
SinI GGWCC 2 cut(s) 61, 554
Sse8387I CCTGCAGG 1 cut(s) 562
Sse9I AATT 6 cut(s) 428, 463, 493, 705, 882, 993
SsiI CCGC 3 cut(s) 14, 88, 222
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 180
StyI CCWWGG 1 cut(s) 1059
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 2 cut(s) 200, 915
TaqI TCGA 1 cut(s) 908
TasI AATT 6 cut(s) 428, 463, 493, 705, 882, 993
TatI WGTACW 1 cut(s) 920
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 1 cut(s) 1091
Tru1I TTAA 1 cut(s) 881
Tru9I TTAA 1 cut(s) 881
TscAI CASTG 2 cut(s) 484, 1080
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 4 cut(s) 224, 608, 646, 950
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 3 cut(s) 63, 448, 992
TspGWI ACGGA 1 cut(s) 508
TspRI CASTG 2 cut(s) 484, 1080
Tth111I GACNNNGTC 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 61, 554
XapI RAATTY 1 cut(s) 705
XceI RCATGY 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 811
XspI CTAG 1 cut(s) 311
ZrmI AGTACT 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.