AT5G41160

Member of a family of proteins related to PUP1, a purine transporter. May be involved in the transport of purine and purine derivatives such as cytokinins, across the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010694
Physical Location & Seq
Forward (+)
16477119 .. 16478436
1318 bp
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UTR
Exon/CDS
Intron
AT5G41160.1

Sequence Viewer

Length: 1095 bp
ATGGATGAAGAAGAAGCAATGTTGCTGCTTAAGGAAGAAGATGAAGGAAGACGAAGAACTAGTGTTCCTACACAACTCATGAAACTAAATAGAAGCCAATGGTGGATTCTTGTTTTCATAAGCATCTTCTTTCTCATCTCTGCTCAAGCCATTTCTGTTCTTCTTGGTCGGTTTTATTACAATGAAGGTGGAAACAGTAAATGGATCTCTACTCTTGTTCAAACTGGTGGCTTTCCCATTCTTTATCTCCCTCTTTCTCTCCTTCCTGCTTCACAATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCAAGACTCTGGTTTGGATTTATCTTTCTCTTGGTTTTGCTATTGGTTTAGATAATTTTTTATACTCTGTTGGACTTTTGTATCTCTCTGCTTCAACTTATTCGATTCTATGTGCTTCACAGTTAGCTTTCAATGGTGTCTTCTATTATTACATCAATTCTCAGAAAATCACTTGTTTGATTTTCTTCTCAGTGTTGTTTCTCTCTATCTCTGCTGTGTTGGTTTCTCTTGACGATGATTCAAATAGCCCATCAGGAGATTCTAAGTGGAGTTACTTGATTGGGTGTTTCTGTGCAGTTTTTGCTTCTCTTATCTATTCTCTTCAGCTCTCTCTTATGCAGTTTTCTTTCGAGAAGGTTCTCAAGAGTGAGACTCTCTCTATGGTTCTCGAGATGCAAATCTATACGTCGCTTGTGGCTTCTTGTGTAGCGGTTATCGGATTGTTCGCAAGCGGGGAATGGATGTTGTTGAGTGTGGAGATGGAAGAGTTTCAGGAAGGTCAAGTCATTTATGTTTTGACTTTGGTCGGGGCAGCGGTTTCGTGTCAATTGGGTTGTGTAGGAGCCGTGTCGCTTATATTTCTGGTGTCTTCGCTGTTTTCAAACCTTATTAGTACGCTCTCACTCATTGTTACGCCTCTCGCGGCCATTGCGGTGTTCCATGACAAGCTGACTGAGGTTAAGATGGTGGCGATGCCCATCGCCTTCACAGGATTCACGTTTTATATCTACCAGAACTATCTTGATGACTTGAAAGTACAAAGAGCACGAGAAGCTCAGGCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

364

Amino Acids

40.33

Weight (kDa)

4.68

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 36 - 353 9.9e-124 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 950
AciI CCGC 5 cut(s) 737, 759, 842, 950, 959
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 951
AcuI CTGAAG 1 cut(s) 614
AfaI GTAC 2 cut(s) 922, 1065
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 7 cut(s) 221, 310, 402, 439, 549, 909, 1060
AhlI ACTAGT 1 cut(s) 59
AjuI GAANNNNNNNTTGG 2 cut(s) 90, 122
AloI GAACNNNNNNTCC 2 cut(s) 49, 81
AluBI AGCT 4 cut(s) 434, 634, 976, 1082
AluI AGCT 4 cut(s) 434, 634, 976, 1082
Alw21I GWGCWC 1 cut(s) 1075
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 695
AoxI GGCC 2 cut(s) 951, 1086
ApeKI GCWGC 2 cut(s) 25, 839
Asp700I GAANNNNTTC 1 cut(s) 795
AvaI CYCGRG 1 cut(s) 695
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BauI CACGAG 1 cut(s) 1074
BbsI GAAGAC 3 cut(s) 55, 439, 888
Bbv12I GWGCWC 1 cut(s) 1075
BbvI GCAGC 2 cut(s) 12, 851
BccI CCATC 4 cut(s) 565, 781, 985, 1013
BceAI ACGGC 1 cut(s) 857
BcgI CGANNNNNNTGC 2 cut(s) 828, 862
BcoDI GTCTC 1 cut(s) 671
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 1 cut(s) 60
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 3 cut(s) 26, 840, 951
BlsI GCNGC 3 cut(s) 27, 841, 952
BmeT110I CYCGRG 1 cut(s) 695
BmiI GGNNCC 1 cut(s) 871
BmsI GCATC 3 cut(s) 132, 690, 990
BoxI GACNNNNGTC 1 cut(s) 830
BpiI GAAGAC 3 cut(s) 55, 439, 888
BplI GAGNNNNNCTC 4 cut(s) 664, 696, 668, 700
Bpu10I CCTNAGC 1 cut(s) 1083
BpuEI CTTGAG 2 cut(s) 129, 653
BsaBI GATNNNNATC 2 cut(s) 704, 1004
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 2 cut(s) 24, 954
Bse8I GATNNNNATC 2 cut(s) 704, 1004
BseGI GGATG 2 cut(s) 10, 774
BseJI GATNNNNATC 2 cut(s) 704, 1004
BseMI GCAATG 2 cut(s) 24, 954
BseMII CTCAG 3 cut(s) 482, 510, 972
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 12, 851
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 1 cut(s) 950
BshFI GGCC 2 cut(s) 953, 1088
BsiHKAI GWGCWC 1 cut(s) 1075
BsiHKCI CYCGRG 1 cut(s) 695
BsmAI GTCTC 1 cut(s) 671
BsnI GGCC 2 cut(s) 953, 1088
BsoBI CYCGRG 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 1075
Bsp143I GATC 1 cut(s) 204
BspACI CCGC 5 cut(s) 737, 759, 842, 950, 959
BspANI GGCC 2 cut(s) 953, 1088
BspCNI CTCAG 3 cut(s) 481, 509, 973
BspFNI CGCG 1 cut(s) 950
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 1 cut(s) 871
BspPI GGATC 1 cut(s) 212
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 2 cut(s) 24, 954
BsrI ACTGG 1 cut(s) 229
BssMI GATC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 1074
Bst2BI CACGAG 1 cut(s) 1074
Bst4CI ACNGT 2 cut(s) 197, 429
Bst6I CTCTTC 2 cut(s) 633, 786
BstAFI CTTAAG 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 608
BstC8I GCNNGC 1 cut(s) 757
BstDEI CTNAG 5 cut(s) 468, 496, 570, 981, 1083
BstF5I GGATG 2 cut(s) 10, 774
BstFNI CGCG 1 cut(s) 950
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 3 cut(s) 608, 956, 1079
BstPAI GACNNNNGTC 1 cut(s) 830
BstUI CGCG 1 cut(s) 950
BstV1I GCAGC 2 cut(s) 12, 851
BstV2I GAAGAC 3 cut(s) 55, 439, 888
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BsuRI GGCC 2 cut(s) 953, 1088
BtgZI GCGATG 2 cut(s) 991, 1013
BtsCI GGATG 2 cut(s) 10, 774
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 757
CciI TCATGA 1 cut(s) 78
Csp6I GTAC 2 cut(s) 921, 1064
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 2 cut(s) 79, 968
CviQI GTAC 2 cut(s) 921, 1064
DdeI CTNAG 5 cut(s) 468, 496, 570, 981, 1083
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
EaeI YGGCCR 1 cut(s) 951
Eam1104I CTCTTC 2 cut(s) 633, 786
EarI CTCTTC 2 cut(s) 633, 786
Eco57I CTGAAG 1 cut(s) 614
Eco88I CYCGRG 1 cut(s) 695
FaeI CATG 2 cut(s) 82, 971
FatI CATG 2 cut(s) 78, 967
FauI CCCGC 1 cut(s) 752
Fnu4HI GCNGC 3 cut(s) 26, 840, 951
FokI GGATG 2 cut(s) 17, 781
Fsp4HI GCNGC 3 cut(s) 26, 840, 951
FspBI CTAG 1 cut(s) 60
GluI GCNGC 3 cut(s) 26, 840, 951
HaeIII GGCC 2 cut(s) 953, 1088
Hin1II CATG 2 cut(s) 82, 971
HinfI GANTC 7 cut(s) 106, 313, 412, 545, 566, 679, 1020
Hpy188I TCNGA 2 cut(s) 471, 746
Hpy99I CGWCG 1 cut(s) 718
HpyAV CCTTC 6 cut(s) 38, 179, 272, 655, 797, 1021
HpyCH4III ACNGT 2 cut(s) 197, 429
HpyCH4IV ACGT 2 cut(s) 713, 1025
HpyCH4V TGCA 3 cut(s) 602, 646, 703
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 956, 1079
HpyF3I CTNAG 5 cut(s) 468, 496, 570, 981, 1083
HpySE526I ACGT 2 cut(s) 713, 1025
Hsp92II CATG 2 cut(s) 82, 971
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 869
LpnPI CCDG 9 cut(s) 210, 279, 302, 546, 785, 875, 1002, 1052, 1070
Lsp1109I GCAGC 2 cut(s) 12, 851
LweI GCATC 3 cut(s) 132, 690, 990
MaeI CTAG 1 cut(s) 60
MaeII ACGT 2 cut(s) 713, 1025
MaeIII GTNAC 2 cut(s) 578, 937
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MfeI CAATTG 1 cut(s) 854
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 1075
MluCI AATT 3 cut(s) 361, 463, 854
MlyI GAGTC 2 cut(s) 307, 673
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 3 cut(s) 261, 954, 976
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 2 cut(s) 30, 987
MslI CAYNNNNRTG 1 cut(s) 959
MspA1I CMGCKG 1 cut(s) 842
MspCI CTTAAG 1 cut(s) 29
MunI CAATTG 1 cut(s) 854
MvnI CGCG 1 cut(s) 950
MwoI GCNNNNNNNGC 3 cut(s) 608, 956, 1079
NdeII GATC 1 cut(s) 204
NlaIII CATG 2 cut(s) 82, 971
NlaIV GGNNCC 1 cut(s) 871
PaeR7I CTCGAG 1 cut(s) 695
PagI TCATGA 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 5 cut(s) 106, 412, 545, 566, 1020
PkrI GCNGC 3 cut(s) 27, 841, 952
PleI GAGTC 2 cut(s) 307, 673
PpsI GAGTC 2 cut(s) 307, 673
PshAI GACNNNNGTC 1 cut(s) 830
PspN4I GGNNCC 1 cut(s) 871
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 2 cut(s) 922, 1065
RsaNI GTAC 2 cut(s) 921, 1064
RseI CAYNNNNRTG 1 cut(s) 959
SaqAI TTAA 2 cut(s) 30, 987
SatI GCNGC 3 cut(s) 26, 840, 951
Sau3AI GATC 1 cut(s) 204
SchI GAGTC 2 cut(s) 307, 673
SduI GDGCHC 1 cut(s) 1075
SfaNI GCATC 3 cut(s) 132, 690, 990
Sfr274I CTCGAG 1 cut(s) 695
SlaI CTCGAG 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 959
SmlI CTYRAG 4 cut(s) 29, 144, 668, 695
SmoI CTYRAG 4 cut(s) 29, 144, 668, 695
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 3 cut(s) 361, 463, 854
SsiI CCGC 5 cut(s) 737, 759, 842, 950, 959
SspMI CTAG 1 cut(s) 60
TaaI ACNGT 2 cut(s) 197, 429
TaiI ACGT 2 cut(s) 716, 1028
TaqI TCGA 3 cut(s) 410, 657, 696
TasI AATT 3 cut(s) 361, 463, 854
TatI WGTACW 1 cut(s) 1063
TauI GCSGC 1 cut(s) 953
TfiI GAWTC 5 cut(s) 106, 412, 545, 566, 1020
Tru1I TTAA 2 cut(s) 30, 987
Tru9I TTAA 2 cut(s) 30, 987
TscAI CASTG 1 cut(s) 504
TseI GCWGC 2 cut(s) 25, 839
TspDTI ATGAA 5 cut(s) 21, 57, 95, 106, 198
TspRI CASTG 1 cut(s) 504
Vha464I CTTAAG 1 cut(s) 29
XhoI CTCGAG 1 cut(s) 695
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.