AT4G08790

Nitrilase-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015452
Physical Location & Seq
Reverse (-)
5608148 .. 5611215
3068 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08790.1

Sequence Viewer

Length: 924 bp
ATGAACGCTTACTCTGTTTCTCTCGACTTCACTAAACCTTCTCTATTCACACGCATCACTCTCTCTTCTCAGATTCCTCTAACCATGGCCACCACTGTAAACAAAACAGTTCGTGTCGCAGCTGCTCAAATGACATCGGTCAATGATCTAATGACCAACTTCGCCACTTGCTCTCGTCTCGTTCAAGAAGCAGCATTGGCTGGTGCAAAGCTGATTTGCTTCCCTGAGAACTTCTCTTTTGTGGGAGATAAGGAAGGAGAGAGTGTGAAAATTGCTGAACCATTAGATGGCCCAGTGATGGAACGGTATTGCTCTCTCGCCAGGGATTCAAATATATGGTTGTCTCTTGGAGGTTTTCAAGAGAGATTTGATGATACTCATTTGTGTAATACACATGTTGTGATCGATGATGCTGGAATGATCCGAGACACTTATCAGAAAATGCATTTGTTTGATGTTGATGTTCCTGGTGGAAGCTCGTACAAAGAAAGCAGCTTTACAGTTCCAGGGACAAAGATTGTTTCCGTAGACAGCCCTGTCGGGCGCTTAGGCCTTACTGTATGCTACGATTTGAGGTTCCCTAAGATATATCAGCAACTGAGATTTGAACAGAAAGCTCAGGTTTTACTAGTACCATCAGCTTTCACCAAGGTCACTGGGGAGGCACACTGGGAGATTCTTCTTCGAGCCCGAGCAATTGAAACTCAATGTTATGTCATAGCTGCTGCTCAAGCAGGAAAGCATAATGAGAAAAGAGAAAGTTATGGTGACACACTGATCATTGATCCGTGGGGAACTGTAGTTGGAAGGTTACCTGATCGTGTTTCGACCGGCATTGTCGTGGCAGATATTGATTTTTCATTGATTGACTCAGTGAGAACAAAAATGCCAATAGATAAGCAACGAGTCTCAATAGATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009536 GO:0010035 GO:0010038 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

307

Amino Acids

33.97

Weight (kDa)

5.79

Isoelectric Point (pI)

32.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 38 - 293 9.8e-64 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 287
AccI GTMKAC 1 cut(s) 528
AclWI GGATC 2 cut(s) 415, 779
AcoI YGGCCR 1 cut(s) 87
AfaI GTAC 2 cut(s) 482, 633
AfiI CCNNNNNNNGG 2 cut(s) 287, 298
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 5 cut(s) 185, 330, 359, 608, 701
AhlI ACTAGT 1 cut(s) 628
AjnI CCWGG 3 cut(s) 320, 466, 505
AluBI AGCT 7 cut(s) 122, 211, 477, 495, 617, 641, 722
AluI AGCT 7 cut(s) 122, 211, 477, 495, 617, 641, 722
Alw26I GTCTC 4 cut(s) 182, 348, 420, 913
AlwI GGATC 2 cut(s) 415, 779
AlwNI CAGNNNCTG 1 cut(s) 598
Ama87I CYCGRG 1 cut(s) 690
AoxI GGCC 3 cut(s) 87, 289, 550
ApeKI GCWGC 6 cut(s) 119, 122, 191, 492, 722, 725
AspLEI GCGC 1 cut(s) 546
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 2 cut(s) 637, 779
AvaI CYCGRG 1 cut(s) 690
BalI TGGCCA 1 cut(s) 89
BanII GRGCYC 1 cut(s) 691
BbvI GCAGC 6 cut(s) 109, 131, 203, 504, 709, 712
BccI CCATC 3 cut(s) 281, 292, 643
BciT130I CCWGG 3 cut(s) 322, 468, 507
BclI TGATCA 1 cut(s) 777
BcoDI GTCTC 4 cut(s) 182, 348, 420, 913
BcuI ACTAGT 1 cut(s) 628
BfaI CTAG 1 cut(s) 629
BfmI CTRYAG 1 cut(s) 798
BfoI RGCGCY 1 cut(s) 547
BglII AGATCT 1 cut(s) 916
BisI GCNGC 6 cut(s) 120, 123, 192, 493, 723, 726
BlsI GCNGC 6 cut(s) 121, 124, 193, 494, 724, 727
Bme1390I CCNGG 3 cut(s) 322, 468, 507
BmeT110I CYCGRG 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 1 cut(s) 578
BmrFI CCNGG 3 cut(s) 322, 468, 507
BmrI ACTGGG 3 cut(s) 287, 666, 679
BmsI GCATC 2 cut(s) 63, 400
BmuI ACTGGG 3 cut(s) 287, 666, 679
BoxI GACNNNNGTC 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 218, 250
Bpu10I CCTNAGC 2 cut(s) 547, 618
BpuEI CTTGAG 1 cut(s) 714
Bsa29I ATCGAT 1 cut(s) 405
BsaJI CCNNGG 5 cut(s) 84, 321, 506, 648, 788
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 298
Bse118I RCCGGY 1 cut(s) 830
Bse1I ACTGG 3 cut(s) 293, 661, 674
BseBI CCWGG 3 cut(s) 322, 468, 507
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 5 cut(s) 84, 321, 506, 648, 788
BseLI CCNNNNNNNGG 2 cut(s) 287, 298
BseMII CTCAG 5 cut(s) 83, 216, 590, 632, 885
BseNI ACTGG 3 cut(s) 293, 661, 674
BseXI GCAGC 6 cut(s) 109, 131, 203, 504, 709, 712
Bsh1285I CGRYCG 1 cut(s) 831
BshFI GGCC 3 cut(s) 89, 291, 552
BshVI ATCGAT 1 cut(s) 405
BsiEI CGRYCG 1 cut(s) 831
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 1 cut(s) 831
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 2 cut(s) 287, 298
BsmAI GTCTC 4 cut(s) 182, 348, 420, 913
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 523
BsnI GGCC 3 cut(s) 89, 291, 552
BsoBI CYCGRG 1 cut(s) 690
Bsp1286I GDGCHC 1 cut(s) 691
Bsp143I GATC 7 cut(s) 145, 402, 420, 777, 784, 817, 916
Bsp19I CCATGG 1 cut(s) 84
BspANI GGCC 3 cut(s) 89, 291, 552
BspCNI CTCAG 5 cut(s) 82, 217, 591, 631, 884
BspDI ATCGAT 1 cut(s) 405
BspLI GGNNCC 1 cut(s) 578
BspPI GGATC 2 cut(s) 415, 779
BsrFI RCCGGY 1 cut(s) 830
BsrI ACTGG 3 cut(s) 293, 661, 674
BssAI RCCGGY 1 cut(s) 830
BssECI CCNNGG 5 cut(s) 84, 321, 506, 648, 788
BssMI GATC 7 cut(s) 145, 402, 420, 777, 784, 817, 916
BssT1I CCWWGG 2 cut(s) 84, 648
Bst2UI CCWGG 3 cut(s) 322, 468, 507
Bst4CI ACNGT 6 cut(s) 97, 109, 306, 502, 559, 799
Bst6I CTCTTC 1 cut(s) 70
BstDEI CTNAG 7 cut(s) 69, 225, 547, 582, 599, 618, 871
BstDSI CCRYGG 2 cut(s) 84, 788
BstEII GGTNACC 1 cut(s) 810
BstH2I RGCGCY 1 cut(s) 547
BstHHI GCGC 1 cut(s) 546
BstKTI GATC 7 cut(s) 148, 405, 423, 780, 787, 820, 919
BstMAI GTCTC 4 cut(s) 182, 348, 420, 913
BstMBI GATC 7 cut(s) 145, 402, 420, 777, 784, 817, 916
BstMCI CGRYCG 1 cut(s) 831
BstMWI GCNNNNNNNGC 2 cut(s) 197, 731
BstNI CCWGG 3 cut(s) 322, 468, 507
BstNSI RCATGY 1 cut(s) 398
BstPAI GACNNNNGTC 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 810
BstSCI CCNGG 3 cut(s) 320, 466, 505
BstSFI CTRYAG 1 cut(s) 798
BstV1I GCAGC 6 cut(s) 109, 131, 203, 504, 709, 712
BstX2I RGATCY 1 cut(s) 916
BstYI RGATCY 1 cut(s) 916
Bsu15I ATCGAT 1 cut(s) 405
BsuRI GGCC 3 cut(s) 89, 291, 552
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 2 cut(s) 84, 788
BtsIMutI CAGTG 6 cut(s) 93, 300, 654, 667, 773, 879
CaiI CAGNNNCTG 1 cut(s) 598
CfoI GCGC 1 cut(s) 546
Cfr10I RCCGGY 1 cut(s) 830
Cfr13I GGNCC 1 cut(s) 290
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 2 cut(s) 481, 632
CviAII CATG 2 cut(s) 85, 395
CviQI GTAC 2 cut(s) 481, 632
DdeI CTNAG 7 cut(s) 69, 225, 547, 582, 599, 618, 871
DpnI GATC 7 cut(s) 147, 404, 422, 779, 786, 819, 918
DpnII GATC 7 cut(s) 145, 402, 420, 777, 784, 817, 916
DrdI GACNNNNNNGTC 1 cut(s) 536
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco130I CCWWGG 2 cut(s) 84, 648
Eco147I AGGCCT 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 691
Eco88I CYCGRG 1 cut(s) 690
Eco91I GGTNACC 1 cut(s) 810
EcoO65I GGTNACC 1 cut(s) 810
EcoRII CCWGG 3 cut(s) 320, 466, 505
EcoT14I CCWWGG 2 cut(s) 84, 648
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 691
ErhI CCWWGG 2 cut(s) 84, 648
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 2 cut(s) 88, 398
FaqI GGGAC 1 cut(s) 523
FatI CATG 2 cut(s) 84, 394
FbaI TGATCA 1 cut(s) 777
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 6 cut(s) 120, 123, 192, 493, 723, 726
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 6 cut(s) 120, 123, 192, 493, 723, 726
FspBI CTAG 1 cut(s) 629
GlaI GCGC 1 cut(s) 545
GluI GCNGC 6 cut(s) 120, 123, 192, 493, 723, 726
HaeII RGCGCY 1 cut(s) 547
HaeIII GGCC 3 cut(s) 89, 291, 552
HapII CCGG 1 cut(s) 831
HhaI GCGC 1 cut(s) 546
Hin1II CATG 2 cut(s) 88, 398
Hin6I GCGC 1 cut(s) 544
HinP1I GCGC 1 cut(s) 544
HinfI GANTC 5 cut(s) 73, 326, 676, 869, 906
HpaII CCGG 1 cut(s) 831
HphI GGTGA 2 cut(s) 637, 779
Hpy166II GTNNAC 2 cut(s) 100, 529
Hpy188I TCNGA 4 cut(s) 72, 425, 438, 923
Hpy188III TCNNGA 3 cut(s) 23, 185, 359
Hpy8I GTNNAC 2 cut(s) 100, 529
HpyAV CCTTC 3 cut(s) 48, 248, 801
HpyCH4III ACNGT 6 cut(s) 97, 109, 306, 502, 559, 799
HpyCH4V TGCA 2 cut(s) 206, 445
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 731
HpyF3I CTNAG 7 cut(s) 69, 225, 547, 582, 599, 618, 871
Hsp92II CATG 2 cut(s) 88, 398
HspAI GCGC 1 cut(s) 544
Ksp22I TGATCA 1 cut(s) 777
Kzo9I GATC 7 cut(s) 145, 402, 420, 777, 784, 817, 916
Lsp1109I GCAGC 6 cut(s) 109, 131, 203, 504, 709, 712
LweI GCATC 2 cut(s) 63, 400
MaeI CTAG 1 cut(s) 629
MaeIII GTNAC 3 cut(s) 652, 767, 810
MalI GATC 7 cut(s) 147, 404, 422, 779, 786, 819, 918
MboI GATC 7 cut(s) 145, 402, 420, 777, 784, 817, 916
MboII GAAGA 3 cut(s) 57, 671, 674
MfeI CAATTG 1 cut(s) 696
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 1 cut(s) 691
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 2 cut(s) 270, 696
MluNI TGGCCA 1 cut(s) 89
MlyI GAGTC 2 cut(s) 863, 915
MmeI TCCRAC 1 cut(s) 784
MnlI CCTC 4 cut(s) 87, 344, 567, 655
Mox20I TGGCCA 1 cut(s) 89
Mph1103I ATGCAT 1 cut(s) 447
MscI TGGCCA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 839
Msp20I TGGCCA 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 831
MspR9I CCNGG 3 cut(s) 322, 468, 507
MunI CAATTG 1 cut(s) 696
MvaI CCWGG 3 cut(s) 322, 468, 507
MwoI GCNNNNNNNGC 2 cut(s) 197, 731
NcoI CCATGG 1 cut(s) 84
NdeII GATC 7 cut(s) 145, 402, 420, 777, 784, 817, 916
NlaIII CATG 2 cut(s) 88, 398
NlaIV GGNNCC 1 cut(s) 578
NmuCI GTSAC 2 cut(s) 652, 767
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 1 cut(s) 398
PceI AGGCCT 1 cut(s) 552
PciI ACATGT 1 cut(s) 394
PfeI GAWTC 3 cut(s) 73, 326, 676
PflMI CCANNNNNTGG 1 cut(s) 287
PkrI GCNGC 6 cut(s) 121, 124, 193, 494, 724, 727
PleI GAGTC 2 cut(s) 863, 914
PpsI GAGTC 2 cut(s) 863, 914
PscI ACATGT 1 cut(s) 394
PshAI GACNNNNGTC 1 cut(s) 137
Psp6I CCWGG 3 cut(s) 320, 466, 505
PspEI GGTNACC 1 cut(s) 810
PspGI CCWGG 3 cut(s) 320, 466, 505
PspN4I GGNNCC 1 cut(s) 578
PspPI GGNCC 1 cut(s) 290
PstNI CAGNNNCTG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 916
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 2 cut(s) 482, 633
RsaNI GTAC 2 cut(s) 481, 632
RseI CAYNNNNRTG 1 cut(s) 839
SatI GCNGC 6 cut(s) 120, 123, 192, 493, 723, 726
Sau3AI GATC 7 cut(s) 145, 402, 420, 777, 784, 817, 916
Sau96I GGNCC 1 cut(s) 290
SchI GAGTC 2 cut(s) 863, 915
ScrFI CCNGG 3 cut(s) 322, 468, 507
SduI GDGCHC 1 cut(s) 691
SfaNI GCATC 2 cut(s) 63, 400
SfcI CTRYAG 1 cut(s) 798
SmiMI CAYNNNNRTG 1 cut(s) 839
SmlI CTYRAG 1 cut(s) 729
SmoI CTYRAG 1 cut(s) 729
SpeI ACTAGT 1 cut(s) 628
Sse9I AATT 2 cut(s) 270, 696
SseBI AGGCCT 1 cut(s) 552
SspMI CTAG 1 cut(s) 629
StuI AGGCCT 1 cut(s) 552
StyD4I CCNGG 3 cut(s) 320, 466, 505
StyI CCWWGG 2 cut(s) 84, 648
TaaI ACNGT 6 cut(s) 97, 109, 306, 502, 559, 799
TaqI TCGA 4 cut(s) 24, 405, 685, 827
TaqII GACCGA 1 cut(s) 127
TasI AATT 2 cut(s) 270, 696
TfiI GAWTC 3 cut(s) 73, 326, 676
TscAI CASTG 6 cut(s) 100, 300, 661, 674, 780, 879
TseFI GTSAC 2 cut(s) 652, 767
TseI GCWGC 6 cut(s) 119, 122, 191, 492, 722, 725
Tsp45I GTSAC 2 cut(s) 652, 767
TspDTI ATGAA 2 cut(s) 17, 849
TspGWI ACGGA 2 cut(s) 514, 777
TspRI CASTG 6 cut(s) 100, 300, 661, 674, 780, 879
Van91I CCANNNNNTGG 1 cut(s) 287
XceI RCATGY 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 1 cut(s) 629
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.