MD07G1183200.v1.1

Nitrilase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015452
Physical Location & Seq
Forward (+)
26333821 .. 26336253
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1183200.v1.1.491

Sequence Viewer

Length: 960 bp
ATGCCGTTTTGTCTTTCCCTACCTAATTATCGTGTCAGCCCACTCCTCCGAGACGGCGCTCCGATCCGTTCCCAGTCCGCCCGCGTCATCATCGCTGGAGAGTCCAACATGGCCGCCAATTCACTCCGAGTCGCCGCCGCCCAAATGACCTCCATCAACGACCTCGCCACCAATTTTGCCACCTGCTCCCGCCTCGTCAAAGAAGCAGCTGCTGCCGGAGCAAAATTGATTTGCTTTCCGGAAAGCTTTTCATTTATTGGTGCCAAAGATGGTGACAGTCTGAAAATAGCAGAACCTCTAGAGGGTCCAATTTTGCAGAAGTACTGCTCCCTGGCAAGAGAATCTGGGATTTGGTTGTCACTTGGAGGGTTCCAAGAAAAGGGGTCGGATGATGAACATTTGTTCAACACCCATGTTGTTGTTGATGATGCTGGGAACATCAGAAGTACTTACAGAAAGATTCAATTGTTTGATGTGGATATTCCTGGTGGAAGGGTGTACAAGGAAGGCAGCTTTACCGAACCTGGAAAGAATGTTGTGGCAGTTGATAGCCCTATTGGCCGTTTGGGTTTGACGGTATGCTATGATCTGAGATTCCCGGAGATTTACCAGCTGCTCAGGTTCCAACATGATGCACAGATTCTATTGGCACCTTCAGCCTTCACCAAAGTAACTGGGCAGGCGCACTGGGAGATTCTTCTACGTGCTCGTGCAATTGAGACTCAATGCTATGTCATAGCTTCTGCACAAGCTGGACATCATAATGATAAAAGAGAAAGCCACGGAGAAACTTTAATTATTGATCCGTGGGGAACAGTTGTTGGCCGACTGCCTGATCGACTGTCAACAGGGATTGCTATAGCCGATATTGATTTCTCATTGATTGACTCAGTGAGAGAAAAAATGCCAATTGCCAAGCACCGGAAGCCTGTTGAGTTCTGGAAATCTGCATCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

320

Amino Acids

34.97

Weight (kDa)

6.71

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 43 - 299 5.2e-63 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 191
Acc36I ACCTGC 1 cut(s) 191
AccB1I GGYRCC 2 cut(s) 260, 649
AccII CGCG 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 238
AciI CCGC 6 cut(s) 78, 82, 114, 135, 138, 190
AclWI GGATC 2 cut(s) 58, 797
AcoI YGGCCR 3 cut(s) 111, 559, 823
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 3 cut(s) 323, 448, 500
AfiI CCNNNNNNNGG 3 cut(s) 302, 379, 921
AgsI TTSAA 2 cut(s) 406, 464
AjnI CCWGG 3 cut(s) 330, 484, 523
AluBI AGCT 6 cut(s) 209, 246, 513, 613, 740, 752
AluI AGCT 6 cut(s) 209, 246, 513, 613, 740, 752
Alw21I GWGCWC 1 cut(s) 709
Alw26I GTCTC 2 cut(s) 45, 713
AlwI GGATC 2 cut(s) 58, 797
AlwNI CAGNNNCTG 1 cut(s) 212
Aor13HI TCCGGA 1 cut(s) 238
AoxI GGCC 3 cut(s) 111, 559, 823
ApeKI GCWGC 5 cut(s) 206, 209, 212, 510, 613
AspLEI GCGC 2 cut(s) 59, 685
AspS9I GGNCC 1 cut(s) 305
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 284, 655
AvaII GGWCC 1 cut(s) 305
BanI GGYRCC 2 cut(s) 260, 649
BauI CACGAG 1 cut(s) 708
Bbv12I GWGCWC 1 cut(s) 709
BbvI GCAGC 5 cut(s) 196, 199, 218, 522, 600
BccI CCATC 2 cut(s) 161, 263
BceAI ACGGC 2 cut(s) 70, 546
BciT130I CCWGG 3 cut(s) 332, 486, 525
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 2 cut(s) 45, 713
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 858
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 191
BglI GCCNNNNNGGC 1 cut(s) 558
BisI GCNGC 8 cut(s) 114, 135, 138, 207, 210, 213, 511, 614
BlsI GCNGC 8 cut(s) 115, 136, 139, 208, 211, 214, 512, 615
BmcAI AGTACT 2 cut(s) 323, 448
Bme1390I CCNGG 4 cut(s) 332, 486, 525, 599
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 5 cut(s) 262, 306, 371, 623, 651
BmrFI CCNGG 4 cut(s) 332, 486, 525, 599
BmrI ACTGGG 3 cut(s) 67, 684, 697
BmsI GCATC 2 cut(s) 418, 622
BmuI ACTGGG 3 cut(s) 67, 684, 697
BpmI CTGGAG 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 617
BpuMI CCSGG 1 cut(s) 599
BsaAI YACGTR 1 cut(s) 704
BsaJI CCNNGG 3 cut(s) 330, 781, 806
BsaWI WCCGGW 2 cut(s) 238, 921
Bsc4I CCNNNNNNNGG 3 cut(s) 302, 379, 921
Bse1I ACTGG 3 cut(s) 73, 679, 692
BseAI TCCGGA 1 cut(s) 238
BseBI CCWGG 3 cut(s) 332, 486, 525
BseDI CCNNGG 3 cut(s) 330, 781, 806
BseGI GGATG 2 cut(s) 394, 950
BseLI CCNNNNNNNGG 3 cut(s) 302, 379, 921
BseMII CTCAG 3 cut(s) 581, 631, 903
BseNI ACTGG 3 cut(s) 73, 679, 692
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 5 cut(s) 196, 199, 218, 522, 600
BseYI CCCAGC 1 cut(s) 431
BsgI GTGCAG 1 cut(s) 729
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 3 cut(s) 113, 561, 825
BshNI GGYRCC 2 cut(s) 260, 649
BsiHKAI GWGCWC 1 cut(s) 709
BsiSI CCGG 4 cut(s) 216, 239, 599, 922
BslI CCNNNNNNNGG 3 cut(s) 302, 379, 921
BsmAI GTCTC 2 cut(s) 45, 713
BsmBI CGTCTC 1 cut(s) 45
BsnI GGCC 3 cut(s) 113, 561, 825
Bsp1286I GDGCHC 1 cut(s) 709
Bsp13I TCCGGA 1 cut(s) 238
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 4 cut(s) 63, 586, 802, 835
BspACI CCGC 6 cut(s) 78, 82, 114, 135, 138, 190
BspANI GGCC 3 cut(s) 113, 561, 825
BspCNI CTCAG 3 cut(s) 582, 630, 902
BspEI TCCGGA 1 cut(s) 238
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 5 cut(s) 262, 306, 371, 623, 651
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 2 cut(s) 58, 797
BspT107I GGYRCC 2 cut(s) 260, 649
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 3 cut(s) 73, 679, 692
BssECI CCNNGG 3 cut(s) 330, 781, 806
BssMI GATC 4 cut(s) 63, 586, 802, 835
BssSI CACGAG 1 cut(s) 708
Bst2BI CACGAG 1 cut(s) 708
Bst2UI CCWGG 3 cut(s) 332, 486, 525
Bst4CI ACNGT 4 cut(s) 278, 577, 817, 843
BstAPI GCANNNNNTGC 1 cut(s) 212
BstAUI TGTACA 1 cut(s) 498
BstBAI YACGTR 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 82, 681
BstDEI CTNAG 3 cut(s) 590, 617, 889
BstDSI CCRYGG 2 cut(s) 781, 806
BstF5I GGATG 2 cut(s) 394, 950
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 685
BstKTI GATC 4 cut(s) 66, 589, 805, 838
BstMAI GTCTC 2 cut(s) 45, 713
BstMBI GATC 4 cut(s) 63, 586, 802, 835
BstMWI GCNNNNNNNGC 5 cut(s) 212, 218, 558, 656, 925
BstNI CCWGG 3 cut(s) 332, 486, 525
BstSCI CCNGG 4 cut(s) 330, 484, 523, 597
BstSFI CTRYAG 1 cut(s) 858
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 5 cut(s) 196, 199, 218, 522, 600
BsuRI GGCC 3 cut(s) 113, 561, 825
BtgI CCRYGG 2 cut(s) 781, 806
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 2 cut(s) 394, 950
BtsIMutI CAGTG 2 cut(s) 685, 897
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 2 cut(s) 82, 681
CaiI CAGNNNCTG 1 cut(s) 212
CfoI GCGC 2 cut(s) 59, 685
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 1 cut(s) 73
Csp6I GTAC 3 cut(s) 322, 447, 499
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 3 cut(s) 109, 413, 629
CviQI GTAC 3 cut(s) 322, 447, 499
DdeI CTNAG 3 cut(s) 590, 617, 889
DpnI GATC 4 cut(s) 65, 588, 804, 837
DpnII GATC 4 cut(s) 63, 586, 802, 835
EaeI YGGCCR 3 cut(s) 111, 559, 823
EciI GGCGGA 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 639
EcoRII CCWGG 3 cut(s) 330, 484, 523
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 3 cut(s) 112, 416, 632
FaiI YATR 9 cut(s) 110, 414, 580, 585, 630, 732, 737, 762, 860
FatI CATG 3 cut(s) 108, 412, 628
FauI CCCGC 2 cut(s) 89, 197
Fnu4HI GCNGC 8 cut(s) 114, 135, 138, 207, 210, 213, 511, 614
FokI GGATG 2 cut(s) 401, 937
Fsp4HI GCNGC 8 cut(s) 114, 135, 138, 207, 210, 213, 511, 614
FspBI CTAG 1 cut(s) 299
GlaI GCGC 2 cut(s) 58, 684
GluI GCNGC 8 cut(s) 114, 135, 138, 207, 210, 213, 511, 614
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 117
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 113, 561, 825
HapII CCGG 4 cut(s) 216, 239, 599, 922
HgaI GACGC 1 cut(s) 73
HhaI GCGC 2 cut(s) 59, 685
Hin1II CATG 3 cut(s) 112, 416, 632
Hin6I GCGC 2 cut(s) 57, 683
HinP1I GCGC 2 cut(s) 57, 683
HincII GTYRAC 1 cut(s) 846
HindII GTYRAC 1 cut(s) 846
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 9 cut(s) 101, 129, 341, 460, 594, 640, 694, 721, 887
HpaII CCGG 4 cut(s) 216, 239, 599, 922
HphI GGTGA 2 cut(s) 284, 655
Hpy166II GTNNAC 2 cut(s) 499, 846
Hpy188I TCNGA 7 cut(s) 50, 63, 128, 282, 388, 443, 591
Hpy188III TCNNGA 3 cut(s) 239, 299, 940
Hpy8I GTNNAC 2 cut(s) 499, 846
HpyAV CCTTC 4 cut(s) 486, 500, 663, 670
HpyCH4III ACNGT 4 cut(s) 278, 577, 817, 843
HpyCH4IV ACGT 1 cut(s) 703
HpyCH4V TGCA 5 cut(s) 316, 635, 713, 746, 950
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 218, 558, 656, 925
HpyF3I CTNAG 3 cut(s) 590, 617, 889
HpySE526I ACGT 1 cut(s) 703
Hsp92II CATG 3 cut(s) 112, 416, 632
HspAI GCGC 2 cut(s) 57, 683
Kpn2I TCCGGA 1 cut(s) 238
Kzo9I GATC 4 cut(s) 63, 586, 802, 835
LmnI GCTCC 4 cut(s) 64, 191, 218, 332
Lsp1109I GCAGC 5 cut(s) 196, 199, 218, 522, 600
LweI GCATC 2 cut(s) 418, 622
MaeI CTAG 1 cut(s) 299
MaeII ACGT 1 cut(s) 703
MaeIII GTNAC 3 cut(s) 272, 357, 670
MalI GATC 4 cut(s) 65, 588, 804, 837
MboI GATC 4 cut(s) 63, 586, 802, 835
MboII GAAGA 1 cut(s) 689
MfeI CAATTG 3 cut(s) 464, 714, 909
MhlI GDGCHC 1 cut(s) 709
MluCI AATT 9 cut(s) 25, 118, 172, 224, 309, 464, 714, 795, 909
MlyI GAGTC 4 cut(s) 110, 138, 715, 881
MmeI TCCRAC 3 cut(s) 129, 366, 649
MnlI CCTC 7 cut(s) 56, 160, 173, 203, 295, 306, 359
MroI TCCGGA 1 cut(s) 238
MseI TTAA 1 cut(s) 794
MslI CAYNNNNRTG 1 cut(s) 762
MspA1I CMGCKG 2 cut(s) 209, 613
MspI CCGG 4 cut(s) 216, 239, 599, 922
MspR9I CCNGG 4 cut(s) 332, 486, 525, 599
MunI CAATTG 3 cut(s) 464, 714, 909
MvaI CCWGG 3 cut(s) 332, 486, 525
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 5 cut(s) 212, 218, 558, 656, 925
NciI CCSGG 1 cut(s) 599
NdeII GATC 4 cut(s) 63, 586, 802, 835
NlaIII CATG 3 cut(s) 112, 416, 632
NlaIV GGNNCC 5 cut(s) 262, 306, 371, 623, 651
NmuCI GTSAC 2 cut(s) 272, 357
PaqCI CACCTGC 1 cut(s) 191
PfeI GAWTC 5 cut(s) 341, 460, 594, 640, 694
PfoI TCCNGGA 1 cut(s) 597
PkrI GCNGC 8 cut(s) 115, 136, 139, 208, 211, 214, 512, 615
PleI GAGTC 4 cut(s) 109, 137, 715, 881
PpsI GAGTC 4 cut(s) 109, 137, 715, 881
Ppu21I YACGTR 1 cut(s) 704
Psp6I CCWGG 3 cut(s) 330, 484, 523
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 3 cut(s) 330, 484, 523
PspN4I GGNNCC 5 cut(s) 262, 306, 371, 623, 651
PspPI GGNCC 1 cut(s) 305
PstNI CAGNNNCTG 1 cut(s) 212
PvuII CAGCTG 2 cut(s) 209, 613
RsaI GTAC 3 cut(s) 323, 448, 500
RsaNI GTAC 3 cut(s) 322, 447, 499
RseI CAYNNNNRTG 1 cut(s) 762
SaqAI TTAA 1 cut(s) 794
SatI GCNGC 8 cut(s) 114, 135, 138, 207, 210, 213, 511, 614
Sau3AI GATC 4 cut(s) 63, 586, 802, 835
Sau96I GGNCC 1 cut(s) 305
ScaI AGTACT 2 cut(s) 323, 448
SchI GAGTC 4 cut(s) 110, 138, 715, 881
ScrFI CCNGG 4 cut(s) 332, 486, 525, 599
SduI GDGCHC 1 cut(s) 709
SfaNI GCATC 2 cut(s) 418, 622
SfcI CTRYAG 1 cut(s) 858
SinI GGWCC 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 762
Sse9I AATT 9 cut(s) 25, 118, 172, 224, 309, 464, 714, 795, 909
SsiI CCGC 6 cut(s) 78, 82, 114, 135, 138, 190
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 4 cut(s) 330, 484, 523, 597
TaaI ACNGT 4 cut(s) 278, 577, 817, 843
TaiI ACGT 1 cut(s) 706
TaqI TCGA 1 cut(s) 838
TasI AATT 9 cut(s) 25, 118, 172, 224, 309, 464, 714, 795, 909
TatI WGTACW 3 cut(s) 321, 446, 498
TauI GCSGC 3 cut(s) 116, 137, 140
TfiI GAWTC 5 cut(s) 341, 460, 594, 640, 694
Tru1I TTAA 1 cut(s) 794
Tru9I TTAA 1 cut(s) 794
TscAI CASTG 2 cut(s) 692, 897
TseFI GTSAC 2 cut(s) 272, 357
TseI GCWGC 5 cut(s) 206, 209, 212, 510, 613
Tsp45I GTSAC 2 cut(s) 272, 357
TspDTI ATGAA 2 cut(s) 240, 408
TspGWI ACGGA 3 cut(s) 56, 795, 798
TspRI CASTG 2 cut(s) 692, 897
VpaK11BI GGWCC 1 cut(s) 305
XbaI TCTAGA 1 cut(s) 298
XspI CTAG 1 cut(s) 299
ZrmI AGTACT 2 cut(s) 323, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.