Rh1AG326700

Nitrilase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0015452
Physical Location & Seq
Forward (+)
55982632 .. 55985753
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG326700.1

Sequence Viewer

Length: 861 bp
ATGTCATTTTGTCTCTCACTAACCAACCCTCACATTACTCCGCTGATTGCTCCGATTCGTCCCAAGTCCCCACGCGCCGTCGCCGGCGGGTCAACCATGGCGGCCAACTCGCTCCGAGTCGCCGCCGCCCAGATGACCTCCATCAACGACCTCGCCACCAATTTCGCCACCTGCTCCCGCCTCGTCAAAGAGGCAGCTGCTAGTGGAGCTAAATTGATTTGCTTCCCTGAAAGCTTCTCCTTTATTGGGGCTAAAGATGGGGATAGTCTCAAAATAGCTGAACCTTTAGAGGGTCCAGTAATGAAACAGTACTGCTCTCTTGCAAGGGAATCTGGGATTTGGTTGTCACTAGGTGGGTTTCAGGAAAAGGGGTCTGATGATGAGCATTTGTTTAACACCCATGTTGTGGTAGATGATGCTGGCAACATCAGAAGCACTTACAGAAAGATTCACTTGTTTGATGTGGATACTCCTGGTGGGAGGTCATACAAGGAAAGTAGCTTTACAGAAGCAGGGAAGAATGTTGTTGCAGTAGATAGCCCTGTGGGACGTCTGGGTTTGACAGTTTGCTATGACCTGAGATTCCCAGAGATTTACCAGCTGCTCAGGTTCCAACATGAGGCACAGATTCTGTTAGTACCTTCAGCCTTTACCAAAGTTACTGGTCAGGCACATTGGGAGGTTCTTCTTCGTGCTCGTGCAATTGAGACTCAATGCTATGTAATAGCTTCTGCACAAGCTGGAAAGCATAATGATAAAAGACAGAGCCATGGCGAAACTTTAATTATTGATCCATGGGGAACTGTTGTTGGTCGACTACCTGGTGAGAATTTAATATCTCTACACGTTTTAAGCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

286

Amino Acids

31.03

Weight (kDa)

7.11

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 39 - 274 4e-60 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 179
AatII GACGTC 1 cut(s) 553
Acc36I ACCTGC 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 406
AccI GTMKAC 1 cut(s) 814
AccII CGCG 1 cut(s) 75
AciI CCGC 6 cut(s) 41, 87, 101, 123, 126, 178
AclWI GGATC 1 cut(s) 785
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 829
AcuI CTGAAG 1 cut(s) 627
AcyI GRCGYC 1 cut(s) 550
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 2 cut(s) 311, 639
AfiI CCNNNNNNNGG 4 cut(s) 246, 290, 406, 619
AflIII ACRYGT 1 cut(s) 844
AjnI CCWGG 2 cut(s) 472, 820
AluBI AGCT 8 cut(s) 197, 209, 234, 278, 501, 601, 728, 740
AluI AGCT 8 cut(s) 197, 209, 234, 278, 501, 601, 728, 740
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 3 cut(s) 17, 272, 701
AlwI GGATC 1 cut(s) 785
AlwNI CAGNNNCTG 1 cut(s) 631
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 3 cut(s) 194, 197, 601
ApoI RAATTY 1 cut(s) 829
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 836
AvaII GGWCC 1 cut(s) 293
BauI CACGAG 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 3 cut(s) 184, 206, 588
BccI CCATC 2 cut(s) 149, 251
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 2 cut(s) 474, 822
BciVI GTATCC 1 cut(s) 460
BcoDI GTCTC 3 cut(s) 17, 272, 701
BfaI CTAG 2 cut(s) 201, 350
BfuAI ACCTGC 1 cut(s) 179
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 602
BlsI GCNGC 6 cut(s) 103, 124, 127, 196, 199, 603
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 2 cut(s) 474, 822
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 294, 611
BmrFI CCNGG 2 cut(s) 474, 822
BmsI GCATC 1 cut(s) 406
Bpu10I CCTNAGC 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 550
BsaJI CCNNGG 3 cut(s) 96, 769, 794
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 290, 406, 619
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 296, 667
BseBI CCWGG 2 cut(s) 474, 822
BseDI CCNNGG 3 cut(s) 96, 769, 794
BseLI CCNNNNNNNGG 4 cut(s) 246, 290, 406, 619
BseMII CTCAG 2 cut(s) 569, 619
BseNI ACTGG 2 cut(s) 296, 667
BseXI GCAGC 3 cut(s) 184, 206, 588
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 3 cut(s) 45, 52, 561
BslI CCNNNNNNNGG 4 cut(s) 246, 290, 406, 619
BsmAI GTCTC 3 cut(s) 17, 272, 701
BsmFI GGGAC 3 cut(s) 45, 52, 561
BsnI GGCC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 697
Bsp143I GATC 1 cut(s) 790
Bsp19I CCATGG 3 cut(s) 96, 769, 794
BspACI CCGC 6 cut(s) 41, 87, 101, 123, 126, 178
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 570, 618
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 294, 611
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 1 cut(s) 785
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 296, 667
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 3 cut(s) 96, 769, 794
BssMI GATC 1 cut(s) 790
BssNI GRCGYC 1 cut(s) 550
BssSI CACGAG 1 cut(s) 696
BssT1I CCWWGG 3 cut(s) 96, 769, 794
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 2 cut(s) 474, 822
Bst4CI ACNGT 3 cut(s) 309, 565, 805
BstACI GRCGYC 1 cut(s) 550
BstC8I GCNNGC 2 cut(s) 85, 421
BstDEI CTNAG 3 cut(s) 578, 605, 858
BstDSI CCRYGG 3 cut(s) 96, 769, 794
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 1 cut(s) 793
BstMAI GTCTC 3 cut(s) 17, 272, 701
BstMBI GATC 1 cut(s) 790
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNI CCWGG 2 cut(s) 474, 822
BstSCI CCNGG 2 cut(s) 472, 820
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 184, 206, 588
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 1 cut(s) 104
BtgI CCRYGG 3 cut(s) 96, 769, 794
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 85, 421
CaiI CAGNNNCTG 1 cut(s) 631
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 293
CsiI ACCWGGT 1 cut(s) 820
Csp6I GTAC 2 cut(s) 310, 638
CviAII CATG 5 cut(s) 97, 401, 617, 770, 795
CviQI GTAC 2 cut(s) 310, 638
DdeI CTNAG 3 cut(s) 578, 605, 858
DpnI GATC 1 cut(s) 792
DpnII GATC 1 cut(s) 790
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 102
Eco130I CCWWGG 3 cut(s) 96, 769, 794
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 627
EcoRII CCWGG 2 cut(s) 472, 820
EcoT14I CCWWGG 3 cut(s) 96, 769, 794
ErhI CCWWGG 3 cut(s) 96, 769, 794
FaeI CATG 5 cut(s) 100, 404, 620, 773, 798
FaiI YATR 9 cut(s) 98, 402, 487, 573, 618, 720, 750, 771, 796
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 3 cut(s) 45, 52, 561
FatI CATG 5 cut(s) 96, 400, 616, 769, 794
FauI CCCGC 2 cut(s) 80, 185
FblI GTMKAC 1 cut(s) 814
Fnu4HI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 602
Fsp4HI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 602
FspBI CTAG 2 cut(s) 201, 350
GlaI GCGC 1 cut(s) 76
GluI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 602
HaeIII GGCC 1 cut(s) 104
HapII CCGG 1 cut(s) 84
HhaI GCGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 550
Hin1II CATG 5 cut(s) 100, 404, 620, 773, 798
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 2 cut(s) 93, 815
HindII GTYRAC 2 cut(s) 93, 815
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 7 cut(s) 55, 117, 329, 448, 582, 628, 709
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 836
Hpy166II GTNNAC 2 cut(s) 93, 815
Hpy188I TCNGA 4 cut(s) 54, 116, 376, 431
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 2 cut(s) 93, 815
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 651
HpyCH4III ACNGT 3 cut(s) 309, 565, 805
HpyCH4IV ACGT 2 cut(s) 550, 846
HpyCH4V TGCA 4 cut(s) 323, 530, 701, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 3 cut(s) 578, 605, 858
HpySE526I ACGT 2 cut(s) 550, 846
Hsp92I GRCGYC 1 cut(s) 550
Hsp92II CATG 5 cut(s) 100, 404, 620, 773, 798
HspAI GCGC 1 cut(s) 75
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Kzo9I GATC 1 cut(s) 790
LmnI GCTCC 4 cut(s) 55, 117, 179, 206
Lsp1109I GCAGC 3 cut(s) 184, 206, 588
LweI GCATC 1 cut(s) 406
MabI ACCWGGT 1 cut(s) 820
MaeI CTAG 2 cut(s) 201, 350
MaeII ACGT 2 cut(s) 550, 846
MaeIII GTNAC 2 cut(s) 345, 658
MalI GATC 1 cut(s) 792
MboI GATC 1 cut(s) 790
MboII GAAGA 3 cut(s) 529, 677, 680
MfeI CAATTG 1 cut(s) 702
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 5 cut(s) 160, 212, 702, 783, 829
MlyI GAGTC 2 cut(s) 126, 703
MmeI TCCRAC 1 cut(s) 637
MnlI CCTC 9 cut(s) 39, 148, 161, 184, 191, 283, 474, 613, 673
MreI CGCCGGCG 1 cut(s) 83
MroNI GCCGGC 1 cut(s) 83
MseI TTAA 4 cut(s) 393, 782, 833, 851
MspA1I CMGCKG 3 cut(s) 43, 197, 601
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 2 cut(s) 474, 822
MunI CAATTG 1 cut(s) 702
MvaI CCWGG 2 cut(s) 474, 822
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 206
NaeI GCCGGC 1 cut(s) 85
NcoI CCATGG 3 cut(s) 96, 769, 794
NdeII GATC 1 cut(s) 790
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 5 cut(s) 100, 404, 620, 773, 798
NlaIV GGNNCC 2 cut(s) 294, 611
NmuCI GTSAC 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 85
PfeI GAWTC 5 cut(s) 55, 329, 448, 582, 628
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 6 cut(s) 103, 124, 127, 196, 199, 603
PleI GAGTC 2 cut(s) 125, 703
PpsI GAGTC 2 cut(s) 125, 703
Psp6I CCWGG 2 cut(s) 472, 820
PspGI CCWGG 2 cut(s) 472, 820
PspN4I GGNNCC 2 cut(s) 294, 611
PspPI GGNCC 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 631
PvuII CAGCTG 2 cut(s) 197, 601
RsaI GTAC 2 cut(s) 311, 639
RsaNI GTAC 2 cut(s) 310, 638
SalI GTCGAC 1 cut(s) 813
SaqAI TTAA 4 cut(s) 393, 782, 833, 851
SatI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 602
Sau3AI GATC 1 cut(s) 790
Sau96I GGNCC 1 cut(s) 293
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 2 cut(s) 126, 703
ScrFI CCNGG 2 cut(s) 474, 822
SduI GDGCHC 1 cut(s) 697
SexAI ACCWGGT 1 cut(s) 820
SfaNI GCATC 1 cut(s) 406
SgrAI CRCCGGYG 1 cut(s) 83
SinI GGWCC 1 cut(s) 293
Sse9I AATT 5 cut(s) 160, 212, 702, 783, 829
SsiI CCGC 6 cut(s) 41, 87, 101, 123, 126, 178
SspMI CTAG 2 cut(s) 201, 350
StyD4I CCNGG 2 cut(s) 472, 820
StyI CCWWGG 3 cut(s) 96, 769, 794
TaaI ACNGT 3 cut(s) 309, 565, 805
TaiI ACGT 2 cut(s) 553, 849
TaqI TCGA 1 cut(s) 814
TasI AATT 5 cut(s) 160, 212, 702, 783, 829
TatI WGTACW 1 cut(s) 309
TauI GCSGC 3 cut(s) 104, 125, 128
TfiI GAWTC 5 cut(s) 55, 329, 448, 582, 628
Tru1I TTAA 4 cut(s) 393, 782, 833, 851
Tru9I TTAA 4 cut(s) 393, 782, 833, 851
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 3 cut(s) 194, 197, 601
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 1 cut(s) 317
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 829
XmiI GTMKAC 1 cut(s) 814
XspI CTAG 2 cut(s) 201, 350
ZraI GACGTC 1 cut(s) 551
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.