Rh1DG319600

Nitrilase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0015452
Physical Location & Seq
Forward (+)
54532162 .. 54534462
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG319600.1

Sequence Viewer

Length: 948 bp
ATGTCATTTTGTCTCTCACTAACCAACTCCCACATTACTCCGCTCATCGCTCCGGTTCGTCCCAAGTCCCGACGCGCCGTCGCCGGCGAGTCAACCATGGCGGCCAACTCGCTCCGAGTCGCCGCCGCCCAGATGACCTCCATCAATGACCTCGCCGCCAATTTCGCCACCTGCTCCCGCCTCGTCAAAGAGGCAGCTGCTAGTGGAGCTAAATTGATTTGCTTCCCTGAAAGCTTCTCCTTTATTGGGGCTAAAGATGGGGATAGTCTCAAAATAGCTGAACCTTTAGAGGGTCCAGTAATGAAACAGTACTGCTCTCTTGCAAGGGAATCTGGGATTTGGTTGTCACTAGGTGGGTTTCAGGAAAAGGGGTCTGATGATGAGCATTTGTTTAACACCCATGTTGTGGTAGATGATGCTGGCAACATCAGAAGCACTTATAGAAAGATTCACTTGTTTGATGTGGATACTCCTGGTGGGAGGTCATACAAGGAAAGTAGCTTTACAGAAGCAGGGAAGAATGTTGTTGCAGTAGATAGCCCTGTGGGACGTTTGGGTTTGACAGTTTGCTATGACCTGAGATTCCCAGAGATTTACCAGCTGCTCAGGTTCCAACATGAGGCACAGATTCTGTTGGTACCTTCAGCCTTTACCAAAGTAACTGGTCAGGCACATTGGGAGGTTCTTCTTCGTGCTCGTGCAATTGAGACTCAATGCTATGTAATAGCTTCTGCACAAGCTGGAAAGCATAATGATAAAAGAGAAAGCCATGGCGAAACTTTAATTATTGATCCATGGGGAACAGTTGTTGGTCGACTACCTGATACTTTGTCAACCGGGATTACTATAGCTGATATTGATTTCTCATTGATCGATTCAGTGAGAGCAAAGATGCCAATTGCCAAGCATCGGAAACCTGTTGAGTTCTGGAAATCTGCATCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

315

Amino Acids

34.38

Weight (kDa)

7.67

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 39 - 295 6.6e-65 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 179
Acc65I GGTACC 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 637
AccB7I CCANNNNNTGG 1 cut(s) 406
AccBSI CCGCTC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 814
AccII CGCG 1 cut(s) 75
AciI CCGC 6 cut(s) 41, 101, 123, 126, 156, 178
AclWI GGATC 1 cut(s) 785
AcoI YGGCCR 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 627
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 2 cut(s) 311, 639
AfiI CCNNNNNNNGG 5 cut(s) 246, 290, 406, 619, 909
AhdI GACNNNNNGTC 1 cut(s) 77
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 9 cut(s) 197, 209, 234, 278, 501, 601, 728, 740, 851
AluI AGCT 9 cut(s) 197, 209, 234, 278, 501, 601, 728, 740, 851
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 3 cut(s) 17, 272, 701
AlwI GGATC 1 cut(s) 785
AlwNI CAGNNNCTG 1 cut(s) 631
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 3 cut(s) 194, 197, 601
Asp718I GGTACC 1 cut(s) 637
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 838
AvaII GGWCC 1 cut(s) 293
BanI GGYRCC 1 cut(s) 637
BauI CACGAG 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 3 cut(s) 184, 206, 588
BccI CCATC 2 cut(s) 149, 251
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 474
BciVI GTATCC 1 cut(s) 460
BcnI CCSGG 1 cut(s) 838
BcoDI GTCTC 3 cut(s) 17, 272, 701
BfaI CTAG 2 cut(s) 201, 350
BfmI CTRYAG 1 cut(s) 846
BfuAI ACCTGC 1 cut(s) 179
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
BlsI GCNGC 7 cut(s) 103, 124, 127, 157, 196, 199, 603
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 2 cut(s) 474, 838
Bme18I GGWCC 1 cut(s) 293
BmeRI GACNNNNNGTC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 3 cut(s) 294, 611, 639
BmrFI CCNGG 2 cut(s) 474, 838
BmsI GCATC 3 cut(s) 406, 882, 916
Bpu10I CCTNAGC 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 838
Bsa29I ATCGAT 1 cut(s) 873
BsaJI CCNNGG 3 cut(s) 96, 769, 794
BsaWI WCCGGW 1 cut(s) 52
Bsc4I CCNNNNNNNGG 5 cut(s) 246, 290, 406, 619, 909
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 296, 667
BseBI CCWGG 1 cut(s) 474
BseCI ATCGAT 1 cut(s) 873
BseDI CCNNGG 3 cut(s) 96, 769, 794
BseLI CCNNNNNNNGG 5 cut(s) 246, 290, 406, 619, 909
BseMII CTCAG 2 cut(s) 569, 619
BseNI ACTGG 2 cut(s) 296, 667
BseXI GCAGC 3 cut(s) 184, 206, 588
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 104
BshNI GGYRCC 1 cut(s) 637
BshVI ATCGAT 1 cut(s) 873
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 3 cut(s) 53, 84, 837
BslFI GGGAC 3 cut(s) 45, 52, 561
BslI CCNNNNNNNGG 5 cut(s) 246, 290, 406, 619, 909
BsmAI GTCTC 3 cut(s) 17, 272, 701
BsmFI GGGAC 3 cut(s) 45, 52, 561
BsnI GGCC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 697
Bsp143I GATC 2 cut(s) 790, 870
Bsp19I CCATGG 3 cut(s) 96, 769, 794
BspACI CCGC 6 cut(s) 41, 101, 123, 126, 156, 178
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 570, 618
BspDI ATCGAT 1 cut(s) 873
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 3 cut(s) 294, 611, 639
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 1 cut(s) 785
BspT107I GGYRCC 1 cut(s) 637
BsrBI CCGCTC 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 296, 667
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 3 cut(s) 96, 769, 794
BssMI GATC 2 cut(s) 790, 870
BssSI CACGAG 1 cut(s) 696
BssT1I CCWWGG 3 cut(s) 96, 769, 794
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 3 cut(s) 309, 565, 805
BstC8I GCNNGC 2 cut(s) 85, 421
BstDEI CTNAG 2 cut(s) 578, 605
BstDSI CCRYGG 3 cut(s) 96, 769, 794
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 2 cut(s) 793, 873
BstMAI GTCTC 3 cut(s) 17, 272, 701
BstMBI GATC 2 cut(s) 790, 870
BstMWI GCNNNNNNNGC 2 cut(s) 164, 206
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 2 cut(s) 472, 836
BstSFI CTRYAG 1 cut(s) 846
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 184, 206, 588
Bsu15I ATCGAT 1 cut(s) 873
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 1 cut(s) 104
BsuTUI ATCGAT 1 cut(s) 873
BtgI CCRYGG 3 cut(s) 96, 769, 794
BtgZI GCGATG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 885
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 85, 421
CaiI CAGNNNCTG 1 cut(s) 631
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 293
ClaI ATCGAT 1 cut(s) 873
CseI GACGC 1 cut(s) 81
Csp6I GTAC 2 cut(s) 310, 638
CviAII CATG 5 cut(s) 97, 401, 617, 770, 795
CviQI GTAC 2 cut(s) 310, 638
DdeI CTNAG 2 cut(s) 578, 605
DpnI GATC 2 cut(s) 792, 872
DpnII GATC 2 cut(s) 790, 870
DraIII CACNNNGTG 1 cut(s) 353
DriI GACNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 102
Eam1105I GACNNNNNGTC 1 cut(s) 77
Eco130I CCWWGG 3 cut(s) 96, 769, 794
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 627
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 3 cut(s) 96, 769, 794
ErhI CCWWGG 3 cut(s) 96, 769, 794
FaeI CATG 5 cut(s) 100, 404, 620, 773, 798
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 3 cut(s) 45, 52, 561
FatI CATG 5 cut(s) 96, 400, 616, 769, 794
FauI CCCGC 1 cut(s) 185
FblI GTMKAC 1 cut(s) 814
Fnu4HI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
Fsp4HI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
FspBI CTAG 2 cut(s) 201, 350
GlaI GCGC 1 cut(s) 76
GluI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
HaeIII GGCC 1 cut(s) 104
HapII CCGG 3 cut(s) 53, 84, 837
HgaI GACGC 1 cut(s) 81
HhaI GCGC 1 cut(s) 77
Hin1II CATG 5 cut(s) 100, 404, 620, 773, 798
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 3 cut(s) 93, 815, 834
HindII GTYRAC 3 cut(s) 93, 815, 834
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 8 cut(s) 89, 117, 329, 448, 582, 628, 709, 875
HpaII CCGG 3 cut(s) 53, 84, 837
Hpy166II GTNNAC 3 cut(s) 93, 815, 834
Hpy188I TCNGA 4 cut(s) 116, 376, 431, 912
Hpy188III TCNNGA 3 cut(s) 69, 362, 928
Hpy8I GTNNAC 3 cut(s) 93, 815, 834
Hpy99I CGWCG 2 cut(s) 75, 83
HpyAV CCTTC 1 cut(s) 651
HpyCH4III ACNGT 3 cut(s) 309, 565, 805
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 5 cut(s) 323, 530, 701, 734, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 206
HpyF3I CTNAG 2 cut(s) 578, 605
HpySE526I ACGT 1 cut(s) 550
Hsp92II CATG 5 cut(s) 100, 404, 620, 773, 798
HspAI GCGC 1 cut(s) 75
KpnI GGTACC 1 cut(s) 641
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Kzo9I GATC 2 cut(s) 790, 870
LmnI GCTCC 4 cut(s) 55, 117, 179, 206
Lsp1109I GCAGC 3 cut(s) 184, 206, 588
LweI GCATC 3 cut(s) 406, 882, 916
MaeI CTAG 2 cut(s) 201, 350
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 2 cut(s) 345, 658
MalI GATC 2 cut(s) 792, 872
MbiI CCGCTC 1 cut(s) 43
MboI GATC 2 cut(s) 790, 870
MboII GAAGA 3 cut(s) 529, 677, 680
MfeI CAATTG 2 cut(s) 702, 897
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 5 cut(s) 160, 212, 702, 783, 897
MlyI GAGTC 3 cut(s) 98, 126, 703
MmeI TCCRAC 1 cut(s) 637
MnlI CCTC 8 cut(s) 148, 161, 184, 191, 283, 474, 613, 673
MreI CGCCGGCG 1 cut(s) 83
MroNI GCCGGC 1 cut(s) 83
MseI TTAA 2 cut(s) 393, 782
MslI CAYNNNNRTG 1 cut(s) 943
MspA1I CMGCKG 2 cut(s) 197, 601
MspI CCGG 3 cut(s) 53, 84, 837
MspR9I CCNGG 2 cut(s) 474, 838
MunI CAATTG 2 cut(s) 702, 897
MvaI CCWGG 1 cut(s) 474
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 164, 206
NaeI GCCGGC 1 cut(s) 85
NciI CCSGG 1 cut(s) 838
NcoI CCATGG 3 cut(s) 96, 769, 794
NdeII GATC 2 cut(s) 790, 870
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 5 cut(s) 100, 404, 620, 773, 798
NlaIV GGNNCC 3 cut(s) 294, 611, 639
NmuCI GTSAC 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 85
PfeI GAWTC 5 cut(s) 329, 448, 582, 628, 875
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 7 cut(s) 103, 124, 127, 157, 196, 199, 603
PleI GAGTC 3 cut(s) 97, 125, 703
PpsI GAGTC 3 cut(s) 97, 125, 703
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 3 cut(s) 294, 611, 639
PspPI GGNCC 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 631
PvuII CAGCTG 2 cut(s) 197, 601
RsaI GTAC 2 cut(s) 311, 639
RsaNI GTAC 2 cut(s) 310, 638
RseI CAYNNNNRTG 1 cut(s) 943
SalI GTCGAC 1 cut(s) 813
SaqAI TTAA 2 cut(s) 393, 782
SatI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
Sau3AI GATC 2 cut(s) 790, 870
Sau96I GGNCC 1 cut(s) 293
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 3 cut(s) 98, 126, 703
ScrFI CCNGG 2 cut(s) 474, 838
SduI GDGCHC 1 cut(s) 697
SfaNI GCATC 3 cut(s) 406, 882, 916
SfcI CTRYAG 1 cut(s) 846
SgrAI CRCCGGYG 1 cut(s) 83
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 943
Sse9I AATT 5 cut(s) 160, 212, 702, 783, 897
SsiI CCGC 6 cut(s) 41, 101, 123, 126, 156, 178
SspMI CTAG 2 cut(s) 201, 350
StyD4I CCNGG 2 cut(s) 472, 836
StyI CCWWGG 3 cut(s) 96, 769, 794
TaaI ACNGT 3 cut(s) 309, 565, 805
TaiI ACGT 1 cut(s) 553
TaqI TCGA 2 cut(s) 814, 873
TasI AATT 5 cut(s) 160, 212, 702, 783, 897
TatI WGTACW 1 cut(s) 309
TauI GCSGC 4 cut(s) 104, 125, 128, 158
TfiI GAWTC 5 cut(s) 329, 448, 582, 628, 875
Tru1I TTAA 2 cut(s) 393, 782
Tru9I TTAA 2 cut(s) 393, 782
TscAI CASTG 1 cut(s) 885
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 3 cut(s) 194, 197, 601
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 1 cut(s) 317
TspRI CASTG 1 cut(s) 885
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 814
XspI CTAG 2 cut(s) 201, 350
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.