Rmu_ssc0000112.1_g000034

Nitrilase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000112.1
OG0015452
Physical Location & Seq
Reverse (-)
169418 .. 171718
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000112.1_g000034.1.cds

Sequence Viewer

Length: 948 bp
atgtcattttgtctctcactaaccaactctcacattactccgctgattgctccgattcgtcccaagtccccacgcgccgtcgccggcgggtcaaccatggcggccagctcgctccgagtcgccgccgcccagatgacctccatcaacgacctcgccgccaatttcgccacctgctcccgcctcgtcaaagaggcagctgctagtggagctaaattgatttgcttccctgaaagcttctcctttattggtgctaaagatggggatagtctcaaaatagctgaacctttagagggtccagtaatgaaacagtactgctctcttgcaagggaatctgggatttggttgtcactaggtgggtttcaggaaaaggggtctgatgatgagcaattgtttaacacccatgttgtggttgatgatgctggcaacatcagaagcacttacagaaagattcacttgtttgatgtggatactcctggtgggaggtcatacaaggaaagtagctttacagaagcagggaagaatgttgttgcagtagatagccctgtgggacgtttgggtttgacagtttgctatgacctgagattcccagagatttaccagctgctcaggttccaacatgaggcacagattctgttagtaccttcagcctttaccaaagttactggtcaggcacattgggaggttcttcttcgtgctcgtgcaattgagactcaatgctatgtaatagcttctgcacaagctggaaagcataatgataaaagacagagccatggcgaaactttaattattgatccatggggaactgttgttggtcgactacctgatactttgtcaaccgggattactatagctgatattgatttctcattgatcgattcagtgagagcaaagatgccaattgccaagcatcggaaacctgttgagttctggaaatctgcatctctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

315

Amino Acids

34.23

Weight (kDa)

8.17

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 406
AccI GTMKAC 1 cut(s) 814
AccII CGCG 1 cut(s) 75
AciI CCGC 7 cut(s) 41, 87, 101, 123, 126, 156, 178
AclWI GGATC 1 cut(s) 785
AcoI YGGCCR 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 627
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 2 cut(s) 311, 639
AfiI CCNNNNNNNGG 4 cut(s) 290, 406, 619, 909
AjnI CCWGG 1 cut(s) 472
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 3 cut(s) 17, 272, 701
AlwI GGATC 1 cut(s) 785
AlwNI CAGNNNCTG 1 cut(s) 631
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 3 cut(s) 194, 197, 601
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 838
AvaII GGWCC 1 cut(s) 293
BauI CACGAG 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 3 cut(s) 184, 206, 588
BccI CCATC 2 cut(s) 149, 251
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 474
BciVI GTATCC 1 cut(s) 460
BcnI CCSGG 1 cut(s) 838
BcoDI GTCTC 3 cut(s) 17, 272, 701
BfaI CTAG 2 cut(s) 201, 350
BfmI CTRYAG 1 cut(s) 846
BfuAI ACCTGC 1 cut(s) 179
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
BlsI GCNGC 7 cut(s) 103, 124, 127, 157, 196, 199, 603
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 2 cut(s) 474, 838
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 294, 611
BmrFI CCNGG 2 cut(s) 474, 838
BmsI GCATC 3 cut(s) 406, 882, 916
Bpu10I CCTNAGC 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 838
Bsa29I ATCGAT 1 cut(s) 873
BsaJI CCNNGG 3 cut(s) 96, 769, 794
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 406, 619, 909
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 296, 667
BseBI CCWGG 1 cut(s) 474
BseCI ATCGAT 1 cut(s) 873
BseDI CCNNGG 3 cut(s) 96, 769, 794
BseLI CCNNNNNNNGG 4 cut(s) 290, 406, 619, 909
BseMII CTCAG 2 cut(s) 569, 619
BseNI ACTGG 2 cut(s) 296, 667
BseXI GCAGC 3 cut(s) 184, 206, 588
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 104
BshVI ATCGAT 1 cut(s) 873
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 2 cut(s) 84, 837
BslFI GGGAC 3 cut(s) 45, 52, 561
BslI CCNNNNNNNGG 4 cut(s) 290, 406, 619, 909
BsmAI GTCTC 3 cut(s) 17, 272, 701
BsmFI GGGAC 3 cut(s) 45, 52, 561
BsnI GGCC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 697
Bsp143I GATC 2 cut(s) 790, 870
Bsp19I CCATGG 3 cut(s) 96, 769, 794
BspACI CCGC 7 cut(s) 41, 87, 101, 123, 126, 156, 178
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 570, 618
BspDI ATCGAT 1 cut(s) 873
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 294, 611
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 1 cut(s) 785
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 296, 667
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 3 cut(s) 96, 769, 794
BssMI GATC 2 cut(s) 790, 870
BssSI CACGAG 1 cut(s) 696
BssT1I CCWWGG 3 cut(s) 96, 769, 794
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 3 cut(s) 309, 565, 805
BstC8I GCNNGC 4 cut(s) 85, 106, 110, 421
BstDEI CTNAG 2 cut(s) 578, 605
BstDSI CCRYGG 3 cut(s) 96, 769, 794
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 2 cut(s) 793, 873
BstMAI GTCTC 3 cut(s) 17, 272, 701
BstMBI GATC 2 cut(s) 790, 870
BstMWI GCNNNNNNNGC 2 cut(s) 164, 206
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 2 cut(s) 472, 836
BstSFI CTRYAG 1 cut(s) 846
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 184, 206, 588
Bsu15I ATCGAT 1 cut(s) 873
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 1 cut(s) 104
BsuTUI ATCGAT 1 cut(s) 873
BtgI CCRYGG 3 cut(s) 96, 769, 794
BtsIMutI CAGTG 1 cut(s) 885
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 4 cut(s) 85, 106, 110, 421
CaiI CAGNNNCTG 1 cut(s) 631
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 293
ClaI ATCGAT 1 cut(s) 873
Csp6I GTAC 2 cut(s) 310, 638
CviAII CATG 5 cut(s) 97, 401, 617, 770, 795
CviQI GTAC 2 cut(s) 310, 638
DdeI CTNAG 2 cut(s) 578, 605
DpnI GATC 2 cut(s) 792, 872
DpnII GATC 2 cut(s) 790, 870
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 102
Eco130I CCWWGG 3 cut(s) 96, 769, 794
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 627
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 3 cut(s) 96, 769, 794
ErhI CCWWGG 3 cut(s) 96, 769, 794
FaeI CATG 5 cut(s) 100, 404, 620, 773, 798
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 3 cut(s) 45, 52, 561
FatI CATG 5 cut(s) 96, 400, 616, 769, 794
FauI CCCGC 2 cut(s) 80, 185
FblI GTMKAC 1 cut(s) 814
Fnu4HI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
Fsp4HI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
FspBI CTAG 2 cut(s) 201, 350
GlaI GCGC 1 cut(s) 76
GluI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
HaeIII GGCC 1 cut(s) 104
HapII CCGG 2 cut(s) 84, 837
HhaI GCGC 1 cut(s) 77
Hin1II CATG 5 cut(s) 100, 404, 620, 773, 798
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 3 cut(s) 93, 815, 834
HindII GTYRAC 3 cut(s) 93, 815, 834
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 8 cut(s) 55, 117, 329, 448, 582, 628, 709, 875
HpaII CCGG 2 cut(s) 84, 837
Hpy166II GTNNAC 3 cut(s) 93, 815, 834
Hpy188I TCNGA 5 cut(s) 54, 116, 376, 431, 912
Hpy188III TCNNGA 2 cut(s) 362, 928
Hpy8I GTNNAC 3 cut(s) 93, 815, 834
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 651
HpyCH4III ACNGT 3 cut(s) 309, 565, 805
HpyCH4IV ACGT 1 cut(s) 550
HpyCH4V TGCA 5 cut(s) 323, 530, 701, 734, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 206
HpyF3I CTNAG 2 cut(s) 578, 605
HpySE526I ACGT 1 cut(s) 550
Hsp92II CATG 5 cut(s) 100, 404, 620, 773, 798
HspAI GCGC 1 cut(s) 75
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Kzo9I GATC 2 cut(s) 790, 870
LmnI GCTCC 4 cut(s) 55, 117, 179, 206
Lsp1109I GCAGC 3 cut(s) 184, 206, 588
LweI GCATC 3 cut(s) 406, 882, 916
MaeI CTAG 2 cut(s) 201, 350
MaeII ACGT 1 cut(s) 550
MaeIII GTNAC 2 cut(s) 345, 658
MalI GATC 2 cut(s) 792, 872
MboI GATC 2 cut(s) 790, 870
MboII GAAGA 3 cut(s) 529, 677, 680
MfeI CAATTG 3 cut(s) 386, 702, 897
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 6 cut(s) 160, 212, 386, 702, 783, 897
MlyI GAGTC 2 cut(s) 126, 703
MmeI TCCRAC 1 cut(s) 637
MnlI CCTC 8 cut(s) 148, 161, 184, 191, 283, 474, 613, 673
MreI CGCCGGCG 1 cut(s) 83
MroNI GCCGGC 1 cut(s) 83
MseI TTAA 2 cut(s) 393, 782
MslI CAYNNNNRTG 1 cut(s) 943
MspA1I CMGCKG 3 cut(s) 43, 197, 601
MspI CCGG 2 cut(s) 84, 837
MspR9I CCNGG 2 cut(s) 474, 838
MunI CAATTG 3 cut(s) 386, 702, 897
MvaI CCWGG 1 cut(s) 474
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 164, 206
NaeI GCCGGC 1 cut(s) 85
NciI CCSGG 1 cut(s) 838
NcoI CCATGG 3 cut(s) 96, 769, 794
NdeII GATC 2 cut(s) 790, 870
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 5 cut(s) 100, 404, 620, 773, 798
NlaIV GGNNCC 2 cut(s) 294, 611
NmuCI GTSAC 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 85
PfeI GAWTC 6 cut(s) 55, 329, 448, 582, 628, 875
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 7 cut(s) 103, 124, 127, 157, 196, 199, 603
PleI GAGTC 2 cut(s) 125, 703
PpsI GAGTC 2 cut(s) 125, 703
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 2 cut(s) 294, 611
PspPI GGNCC 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 631
PvuII CAGCTG 2 cut(s) 197, 601
RsaI GTAC 2 cut(s) 311, 639
RsaNI GTAC 2 cut(s) 310, 638
RseI CAYNNNNRTG 1 cut(s) 943
SalI GTCGAC 1 cut(s) 813
SaqAI TTAA 2 cut(s) 393, 782
SatI GCNGC 7 cut(s) 102, 123, 126, 156, 195, 198, 602
Sau3AI GATC 2 cut(s) 790, 870
Sau96I GGNCC 1 cut(s) 293
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 2 cut(s) 126, 703
ScrFI CCNGG 2 cut(s) 474, 838
SduI GDGCHC 1 cut(s) 697
SfaNI GCATC 3 cut(s) 406, 882, 916
SfcI CTRYAG 1 cut(s) 846
SgrAI CRCCGGYG 1 cut(s) 83
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 943
Sse9I AATT 6 cut(s) 160, 212, 386, 702, 783, 897
SsiI CCGC 7 cut(s) 41, 87, 101, 123, 126, 156, 178
SspMI CTAG 2 cut(s) 201, 350
StyD4I CCNGG 2 cut(s) 472, 836
StyI CCWWGG 3 cut(s) 96, 769, 794
TaaI ACNGT 3 cut(s) 309, 565, 805
TaiI ACGT 1 cut(s) 553
TaqI TCGA 2 cut(s) 814, 873
TasI AATT 6 cut(s) 160, 212, 386, 702, 783, 897
TatI WGTACW 1 cut(s) 309
TauI GCSGC 4 cut(s) 104, 125, 128, 158
TfiI GAWTC 6 cut(s) 55, 329, 448, 582, 628, 875
Tru1I TTAA 2 cut(s) 393, 782
Tru9I TTAA 2 cut(s) 393, 782
TscAI CASTG 1 cut(s) 885
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 3 cut(s) 194, 197, 601
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 1 cut(s) 317
TspRI CASTG 1 cut(s) 885
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 814
XspI CTAG 2 cut(s) 201, 350
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.