Rw1G028940

Nitrilase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015452
Physical Location & Seq
Forward (+)
56630579 .. 56632878
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028940.1

Sequence Viewer

Length: 882 bp
ATGTCATTTTGTCTCTCACTAACTAACCCTCACATTACTCCGCTCATTGCTCCGATTCGTCCCAAGTCCCCACGCGCCGACGCCGGCGGGTCAACCATGGCGGCCAACTCGCTCCGAGTCGCCGCCGCCCAGATGACCTCCATCAACGACCTCGCCACCAATTTCGCCACCTGCTCCCGCCTCGTCAAAGAGGCAGCTGCTAGTGGAGCTAAATTGATTTGCTTCCCTGAAAGCTTCTCCTTTATTGGGGCTAAAGATGGGGATAGTCTCAAAATAGCTGAACCTTTAGAGGGTCCAGTAATGAAACAGTACTGCTCTCTTGCAAGGGAATCTGGGATTTGGTTGTCACTAGGTGGGTTTCAGGAAAAGGGGTCTGATGATGAGCATTTGTTTGATGTGGATACTCCTGGTGGGAGGTCATACAAGGAAAGTAGCTTTACAGAAGCAGGGAAGAATGTTGTTGCAGTAGATAGCCCTGTGGGACGTTTGGGTTTGACTGTTTGCTATGACCTGAGATTCCCAGAGATTTACCAGCTGCTCAGGTTCCAACATGAGGCACAGATTCTGTTAGTACCTTCAGCCTTTACCAAAGTAACTGGTCAGGCACATTGGGAGGTTCTTCTTCGTGCTCGTGCAATTGAGACTCAATGCTATGTAATAGCTTCTGCACAAGCTGGAAAGCATAATGATAAAAGAGAGAGCCATGGCGAAACTTTAATTATTGATCCATGGGGAACTGTTGTTGGTCGACTACCTGATACTTTGTCAACCGGGATTACTATAGCTGATATTGATTTCTCATTGATCGATTCAGTGAGAGCAAAGATGCCAATTGCCAAGCATCGGAAACCTGTTGAGTTCTGGAAATCTGCATCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

293

Amino Acids

31.78

Weight (kDa)

6.66

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 39 - 123 1.5e-14 Carbon-nitrogen hydrolase
CN_hydrolase PF00795 129 - 273 8e-34 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 179
AccBSI CCGCTC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 748
AccII CGCG 1 cut(s) 75
AciI CCGC 6 cut(s) 41, 87, 101, 123, 126, 178
AclWI GGATC 1 cut(s) 719
AcoI YGGCCR 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 81
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 2 cut(s) 311, 573
AfiI CCNNNNNNNGG 4 cut(s) 246, 290, 553, 843
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 9 cut(s) 197, 209, 234, 278, 435, 535, 662, 674, 785
AluI AGCT 9 cut(s) 197, 209, 234, 278, 435, 535, 662, 674, 785
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 3 cut(s) 17, 272, 635
AlwI GGATC 1 cut(s) 719
AlwNI CAGNNNCTG 1 cut(s) 565
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 3 cut(s) 194, 197, 535
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 772
AvaII GGWCC 1 cut(s) 293
BauI CACGAG 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 3 cut(s) 184, 206, 522
BccI CCATC 2 cut(s) 149, 251
BciT130I CCWGG 1 cut(s) 408
BciVI GTATCC 1 cut(s) 394
BcnI CCSGG 1 cut(s) 772
BcoDI GTCTC 3 cut(s) 17, 272, 635
BfaI CTAG 2 cut(s) 201, 350
BfmI CTRYAG 1 cut(s) 780
BfuAI ACCTGC 1 cut(s) 179
BfuI GTATCC 1 cut(s) 394
BisI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 536
BlsI GCNGC 6 cut(s) 103, 124, 127, 196, 199, 537
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 2 cut(s) 408, 772
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 294, 545
BmrFI CCNGG 2 cut(s) 408, 772
BmsI GCATC 2 cut(s) 816, 850
Bpu10I CCTNAGC 1 cut(s) 539
BpuMI CCSGG 1 cut(s) 772
Bsa29I ATCGAT 1 cut(s) 807
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 96, 703, 728
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 290, 553, 843
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 296, 601
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 807
BseDI CCNNGG 3 cut(s) 96, 703, 728
BseLI CCNNNNNNNGG 4 cut(s) 246, 290, 553, 843
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 503, 553
BseNI ACTGG 2 cut(s) 296, 601
BseXI GCAGC 3 cut(s) 184, 206, 522
BsgI GTGCAG 1 cut(s) 651
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 104
BshVI ATCGAT 1 cut(s) 807
BsiHKAI GWGCWC 1 cut(s) 631
BsiSI CCGG 2 cut(s) 84, 771
BslFI GGGAC 3 cut(s) 45, 52, 495
BslI CCNNNNNNNGG 4 cut(s) 246, 290, 553, 843
BsmAI GTCTC 3 cut(s) 17, 272, 635
BsmFI GGGAC 3 cut(s) 45, 52, 495
BsnI GGCC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 631
Bsp143I GATC 2 cut(s) 724, 804
Bsp19I CCATGG 3 cut(s) 96, 703, 728
BspACI CCGC 6 cut(s) 41, 87, 101, 123, 126, 178
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 504, 552
BspDI ATCGAT 1 cut(s) 807
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 294, 545
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 1 cut(s) 719
BsrBI CCGCTC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 296, 601
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 3 cut(s) 96, 703, 728
BssMI GATC 2 cut(s) 724, 804
BssNI GRCGYC 1 cut(s) 81
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 3 cut(s) 96, 703, 728
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 309, 499, 739
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 512, 539
BstDSI CCRYGG 3 cut(s) 96, 703, 728
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 2 cut(s) 727, 807
BstMAI GTCTC 3 cut(s) 17, 272, 635
BstMBI GATC 2 cut(s) 724, 804
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNI CCWGG 1 cut(s) 408
BstSCI CCNGG 2 cut(s) 406, 770
BstSFI CTRYAG 1 cut(s) 780
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 184, 206, 522
Bsu15I ATCGAT 1 cut(s) 807
BsuI GTATCC 1 cut(s) 394
BsuRI GGCC 1 cut(s) 104
BsuTUI ATCGAT 1 cut(s) 807
BtgI CCRYGG 3 cut(s) 96, 703, 728
BtsIMutI CAGTG 1 cut(s) 819
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 85
CaiI CAGNNNCTG 1 cut(s) 565
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 293
ClaI ATCGAT 1 cut(s) 807
CseI GACGC 1 cut(s) 89
Csp6I GTAC 2 cut(s) 310, 572
CviAII CATG 4 cut(s) 97, 551, 704, 729
CviQI GTAC 2 cut(s) 310, 572
DdeI CTNAG 2 cut(s) 512, 539
DpnI GATC 2 cut(s) 726, 806
DpnII GATC 2 cut(s) 724, 804
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 102
Eco130I CCWWGG 3 cut(s) 96, 703, 728
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 561
EcoRII CCWGG 1 cut(s) 406
EcoT14I CCWWGG 3 cut(s) 96, 703, 728
ErhI CCWWGG 3 cut(s) 96, 703, 728
FaeI CATG 4 cut(s) 100, 554, 707, 732
FaqI GGGAC 3 cut(s) 45, 52, 495
FatI CATG 4 cut(s) 96, 550, 703, 728
FauI CCCGC 2 cut(s) 80, 185
FblI GTMKAC 1 cut(s) 748
Fnu4HI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 536
Fsp4HI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 536
FspBI CTAG 2 cut(s) 201, 350
GlaI GCGC 1 cut(s) 76
GluI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 536
HaeIII GGCC 1 cut(s) 104
HapII CCGG 2 cut(s) 84, 771
HgaI GACGC 1 cut(s) 89
HhaI GCGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 4 cut(s) 100, 554, 707, 732
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 3 cut(s) 93, 749, 768
HindII GTYRAC 3 cut(s) 93, 749, 768
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 7 cut(s) 55, 117, 329, 516, 562, 643, 809
HpaII CCGG 2 cut(s) 84, 771
Hpy166II GTNNAC 3 cut(s) 93, 749, 768
Hpy188I TCNGA 4 cut(s) 54, 116, 376, 846
Hpy188III TCNNGA 2 cut(s) 362, 862
Hpy8I GTNNAC 3 cut(s) 93, 749, 768
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 585
HpyCH4III ACNGT 3 cut(s) 309, 499, 739
HpyCH4IV ACGT 1 cut(s) 484
HpyCH4V TGCA 5 cut(s) 323, 464, 635, 668, 872
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 2 cut(s) 512, 539
HpySE526I ACGT 1 cut(s) 484
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 4 cut(s) 100, 554, 707, 732
HspAI GCGC 1 cut(s) 75
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Kzo9I GATC 2 cut(s) 724, 804
LmnI GCTCC 4 cut(s) 55, 117, 179, 206
Lsp1109I GCAGC 3 cut(s) 184, 206, 522
LweI GCATC 2 cut(s) 816, 850
MaeI CTAG 2 cut(s) 201, 350
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 2 cut(s) 345, 592
MalI GATC 2 cut(s) 726, 806
MbiI CCGCTC 1 cut(s) 43
MboI GATC 2 cut(s) 724, 804
MboII GAAGA 3 cut(s) 463, 611, 614
MfeI CAATTG 2 cut(s) 636, 831
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 5 cut(s) 160, 212, 636, 717, 831
MlyI GAGTC 2 cut(s) 126, 637
MmeI TCCRAC 1 cut(s) 571
MnlI CCTC 9 cut(s) 39, 148, 161, 184, 191, 283, 408, 547, 607
MreI CGCCGGCG 1 cut(s) 83
MroNI GCCGGC 1 cut(s) 83
MseI TTAA 1 cut(s) 716
MslI CAYNNNNRTG 1 cut(s) 877
MspA1I CMGCKG 2 cut(s) 197, 535
MspI CCGG 2 cut(s) 84, 771
MspR9I CCNGG 2 cut(s) 408, 772
MunI CAATTG 2 cut(s) 636, 831
MvaI CCWGG 1 cut(s) 408
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 206
NaeI GCCGGC 1 cut(s) 85
NciI CCSGG 1 cut(s) 772
NcoI CCATGG 3 cut(s) 96, 703, 728
NdeII GATC 2 cut(s) 724, 804
NgoMIV GCCGGC 1 cut(s) 83
NlaIII CATG 4 cut(s) 100, 554, 707, 732
NlaIV GGNNCC 2 cut(s) 294, 545
NmuCI GTSAC 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 85
PfeI GAWTC 5 cut(s) 55, 329, 516, 562, 809
PkrI GCNGC 6 cut(s) 103, 124, 127, 196, 199, 537
PleI GAGTC 2 cut(s) 125, 637
PpsI GAGTC 2 cut(s) 125, 637
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 2 cut(s) 294, 545
PspPI GGNCC 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 565
PvuII CAGCTG 2 cut(s) 197, 535
RsaI GTAC 2 cut(s) 311, 573
RsaNI GTAC 2 cut(s) 310, 572
RseI CAYNNNNRTG 1 cut(s) 877
SalI GTCGAC 1 cut(s) 747
SaqAI TTAA 1 cut(s) 716
SatI GCNGC 6 cut(s) 102, 123, 126, 195, 198, 536
Sau3AI GATC 2 cut(s) 724, 804
Sau96I GGNCC 1 cut(s) 293
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 2 cut(s) 126, 637
ScrFI CCNGG 2 cut(s) 408, 772
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 2 cut(s) 816, 850
SfcI CTRYAG 1 cut(s) 780
SgrAI CRCCGGYG 1 cut(s) 83
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 877
Sse9I AATT 5 cut(s) 160, 212, 636, 717, 831
SsiI CCGC 6 cut(s) 41, 87, 101, 123, 126, 178
SspMI CTAG 2 cut(s) 201, 350
StyD4I CCNGG 2 cut(s) 406, 770
StyI CCWWGG 3 cut(s) 96, 703, 728
TaaI ACNGT 3 cut(s) 309, 499, 739
TaiI ACGT 1 cut(s) 487
TaqI TCGA 2 cut(s) 748, 807
TasI AATT 5 cut(s) 160, 212, 636, 717, 831
TatI WGTACW 1 cut(s) 309
TauI GCSGC 3 cut(s) 104, 125, 128
TfiI GAWTC 5 cut(s) 55, 329, 516, 562, 809
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TscAI CASTG 1 cut(s) 819
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 3 cut(s) 194, 197, 535
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 1 cut(s) 317
TspRI CASTG 1 cut(s) 819
VpaK11BI GGWCC 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 748
XspI CTAG 2 cut(s) 201, 350
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.