FvH4_7g20650

Nitrilase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015452
Physical Location & Seq
Forward (+)
16735403 .. 16738326
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20650.t1

Sequence Viewer

Length: 939 bp
ATGTCATTTTGTCTCTCACTACCCAACTCTCGCATCACTCCACCCACCTCTCCGATTCCTCCCAAGTCCAGCGCCGGCGCGTCCAACATGGCGGCCAACTCCCTCCGAGTCGCCGCCGCCCAGATGACCTCCATCAACGACCTCGCCGCCAACTTCGCCACCTGCTCCCGCCTCGTCAAAGAGGCAGCTGCTAGTGGAGCAAAGTTGATTTGCTTCCCTGAAAGCTTCTCCTTTATAGGTGCTAAAGATGGGGATAGTCTCAAAATAGCTGAGCCTTTGGAGGGTCCAGTAATGCAACAGTACTGCTCTCTTGCAAGGGAGTCTGGGATTTGGTTGTCACTAGGAGGGTTTCAGGAGAAGGGGTCTGATGATGAGCATTTGTTTAACACTCATGTTGTGGTTGATGATGGTGGGAATATTAGAATCACTTACAGAAAGATTCACTTGTTTGATGTGGATACTCCTGGTGGGAGGTCATACAAGGAAAGTATCATTACAGAAGCAGGGAAGAATGTTGTTGCAGTAGATAGCCCCGTGGGTCGCTTGGGTTTGACAGTTTGCTATGACCTGAGATTCCCAGAGATATACCAGCTGCTCAGGTTCCAACATGAGGCACAGATTCTGTTGGTACCTTCAGCCTTTACCAAAGTAACTGGTCAGGCACATTGGGAGATTCTTCTTCGTGCTCGTGCAATTGAGACTCAATGTTACGTAATAGCTGCTGCACAAGCGGGAAAGCATAACGATAAAAGAGAAAGCCATGGTGAAACTTTAATTATTGATCCATGGGGAACAGTTGTTGGTCGACTGCCTGATACGCTGTCAACTGGGATTACTATAGCTGATATTGATTTCTCCTTGATCGATTCAGTGAGAGCAAAGATGCCGATTGCCAAGCATCGGAAACCCGTTGAGTTCTGGAAATCAGCATCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

313

Amino Acids

33.9

Weight (kDa)

6.71

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 36 - 292 8.8e-65 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 170
Acc65I GGTACC 1 cut(s) 628
AccB1I GGYRCC 1 cut(s) 628
AccI GTMKAC 1 cut(s) 805
AccII CGCG 1 cut(s) 80
AciI CCGC 6 cut(s) 92, 114, 117, 147, 169, 731
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 618
AfaI GTAC 2 cut(s) 302, 630
AfiI CCNNNNNNNGG 3 cut(s) 281, 610, 900
AjnI CCWGG 1 cut(s) 463
AluBI AGCT 6 cut(s) 188, 225, 269, 592, 719, 842
AluI AGCT 6 cut(s) 188, 225, 269, 592, 719, 842
Alw21I GWGCWC 1 cut(s) 688
Alw26I GTCTC 3 cut(s) 17, 263, 692
AlwI GGATC 1 cut(s) 776
AlwNI CAGNNNCTG 1 cut(s) 622
AoxI GGCC 1 cut(s) 93
ApeKI GCWGC 5 cut(s) 185, 188, 592, 719, 722
Asp718I GGTACC 1 cut(s) 628
AspLEI GCGC 2 cut(s) 74, 80
AspS9I GGNCC 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 776
AvaII GGWCC 1 cut(s) 284
BanI GGYRCC 1 cut(s) 628
BauI CACGAG 1 cut(s) 687
Bbv12I GWGCWC 1 cut(s) 688
BbvI GCAGC 5 cut(s) 175, 197, 579, 706, 709
BccI CCATC 3 cut(s) 140, 242, 401
BciT130I CCWGG 1 cut(s) 465
BciVI GTATCC 1 cut(s) 451
BcoDI GTCTC 3 cut(s) 17, 263, 692
BfaI CTAG 2 cut(s) 192, 341
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 170
BfuI GTATCC 1 cut(s) 451
BisI GCNGC 9 cut(s) 93, 114, 117, 147, 186, 189, 593, 720, 723
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 9 cut(s) 94, 115, 118, 148, 187, 190, 594, 721, 724
BmcAI AGTACT 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 465
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 3 cut(s) 285, 602, 630
BmrFI CCNGG 1 cut(s) 465
BmrI ACTGGG 1 cut(s) 837
BmsI GCATC 3 cut(s) 42, 873, 907
BmuI ACTGGG 1 cut(s) 837
Bpu10I CCTNAGC 1 cut(s) 596
Bpu1102I GCTNAGC 1 cut(s) 270
Bsa29I ATCGAT 1 cut(s) 864
BsaAI YACGTR 1 cut(s) 712
BsaJI CCNNGG 3 cut(s) 534, 760, 785
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 610, 900
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 287, 658, 832
BseBI CCWGG 1 cut(s) 465
BseCI ATCGAT 1 cut(s) 864
BseDI CCNNGG 3 cut(s) 534, 760, 785
BseLI CCNNNNNNNGG 3 cut(s) 281, 610, 900
BseMII CTCAG 3 cut(s) 261, 560, 610
BseNI ACTGG 3 cut(s) 287, 658, 832
BseXI GCAGC 5 cut(s) 175, 197, 579, 706, 709
BsgI GTGCAG 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 628
BshVI ATCGAT 1 cut(s) 864
BsiHKAI GWGCWC 1 cut(s) 688
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 3 cut(s) 281, 610, 900
BsmAI GTCTC 3 cut(s) 17, 263, 692
BsnI GGCC 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 688
Bsp143I GATC 2 cut(s) 781, 861
Bsp1720I GCTNAGC 1 cut(s) 270
Bsp19I CCATGG 2 cut(s) 760, 785
BspACI CCGC 6 cut(s) 92, 114, 117, 147, 169, 731
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 3 cut(s) 262, 561, 609
BspDI ATCGAT 1 cut(s) 864
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 3 cut(s) 285, 602, 630
BspMI ACCTGC 1 cut(s) 170
BspPI GGATC 1 cut(s) 776
BspT107I GGYRCC 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 3 cut(s) 287, 658, 832
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 534, 760, 785
BssMI GATC 2 cut(s) 781, 861
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 2 cut(s) 760, 785
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 3 cut(s) 300, 556, 796
BstBAI YACGTR 1 cut(s) 712
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 3 cut(s) 270, 569, 596
BstDSI CCRYGG 3 cut(s) 534, 760, 785
BstFNI CGCG 1 cut(s) 80
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 80
BstKTI GATC 2 cut(s) 784, 864
BstMAI GTCTC 3 cut(s) 17, 263, 692
BstMBI GATC 2 cut(s) 781, 861
BstMWI GCNNNNNNNGC 4 cut(s) 155, 197, 728, 817
BstNI CCWGG 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 463
BstSFI CTRYAG 1 cut(s) 837
BstSNI TACGTA 1 cut(s) 712
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 5 cut(s) 175, 197, 579, 706, 709
Bsu15I ATCGAT 1 cut(s) 864
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 1 cut(s) 95
BsuTUI ATCGAT 1 cut(s) 864
BtgI CCRYGG 3 cut(s) 534, 760, 785
BtsIMutI CAGTG 1 cut(s) 876
BveI ACCTGC 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 76
CaiI CAGNNNCTG 1 cut(s) 622
CfoI GCGC 2 cut(s) 74, 80
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 284
ClaI ATCGAT 1 cut(s) 864
CseI GACGC 1 cut(s) 69
Csp6I GTAC 2 cut(s) 301, 629
CviAII CATG 5 cut(s) 88, 392, 608, 761, 786
CviQI GTAC 2 cut(s) 301, 629
DdeI CTNAG 3 cut(s) 270, 569, 596
DpnI GATC 2 cut(s) 783, 863
DpnII GATC 2 cut(s) 781, 861
EaeI YGGCCR 1 cut(s) 93
Eco105I TACGTA 1 cut(s) 712
Eco130I CCWWGG 2 cut(s) 760, 785
Eco47I GGWCC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 618
EcoRII CCWGG 1 cut(s) 463
EcoT14I CCWWGG 2 cut(s) 760, 785
ErhI CCWWGG 2 cut(s) 760, 785
FaeI CATG 5 cut(s) 91, 395, 611, 764, 789
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FatI CATG 5 cut(s) 87, 391, 607, 760, 785
FauI CCCGC 2 cut(s) 176, 724
FblI GTMKAC 1 cut(s) 805
Fnu4HI GCNGC 9 cut(s) 93, 114, 117, 147, 186, 189, 593, 720, 723
Fsp4HI GCNGC 9 cut(s) 93, 114, 117, 147, 186, 189, 593, 720, 723
FspBI CTAG 2 cut(s) 192, 341
GlaI GCGC 2 cut(s) 73, 79
GluI GCNGC 9 cut(s) 93, 114, 117, 147, 186, 189, 593, 720, 723
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 95
HapII CCGG 1 cut(s) 75
HgaI GACGC 1 cut(s) 69
HhaI GCGC 2 cut(s) 74, 80
Hin1II CATG 5 cut(s) 91, 395, 611, 764, 789
Hin6I GCGC 2 cut(s) 72, 78
HinP1I GCGC 2 cut(s) 72, 78
HincII GTYRAC 2 cut(s) 806, 825
HindII GTYRAC 2 cut(s) 806, 825
HindIII AAGCTT 1 cut(s) 223
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 776
Hpy166II GTNNAC 2 cut(s) 806, 825
Hpy188I TCNGA 4 cut(s) 54, 107, 367, 903
Hpy188III TCNNGA 2 cut(s) 353, 919
Hpy8I GTNNAC 2 cut(s) 806, 825
HpyAV CCTTC 2 cut(s) 352, 642
HpyCH4III ACNGT 3 cut(s) 300, 556, 796
HpyCH4IV ACGT 1 cut(s) 711
HpyCH4V TGCA 5 cut(s) 295, 314, 521, 692, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 197, 728, 817
HpyF3I CTNAG 3 cut(s) 270, 569, 596
HpySE526I ACGT 1 cut(s) 711
Hsp92II CATG 5 cut(s) 91, 395, 611, 764, 789
HspAI GCGC 2 cut(s) 72, 78
KpnI GGTACC 1 cut(s) 632
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
Kzo9I GATC 2 cut(s) 781, 861
LmnI GCTCC 2 cut(s) 170, 197
Lsp1109I GCAGC 5 cut(s) 175, 197, 579, 706, 709
LweI GCATC 3 cut(s) 42, 873, 907
MaeI CTAG 2 cut(s) 192, 341
MaeII ACGT 1 cut(s) 711
MaeIII GTNAC 3 cut(s) 336, 649, 707
MalI GATC 2 cut(s) 783, 863
MboI GATC 2 cut(s) 781, 861
MboII GAAGA 3 cut(s) 520, 668, 671
MfeI CAATTG 1 cut(s) 693
MhlI GDGCHC 1 cut(s) 688
MluCI AATT 2 cut(s) 693, 774
MlyI GAGTC 3 cut(s) 117, 329, 694
MmeI TCCRAC 2 cut(s) 108, 628
MreI CGCCGGCG 1 cut(s) 74
MroNI GCCGGC 1 cut(s) 74
MseI TTAA 2 cut(s) 384, 773
MslI CAYNNNNRTG 1 cut(s) 934
MspA1I CMGCKG 2 cut(s) 188, 592
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 465
MunI CAATTG 1 cut(s) 693
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 4 cut(s) 155, 197, 728, 817
NaeI GCCGGC 1 cut(s) 76
NcoI CCATGG 2 cut(s) 760, 785
NdeII GATC 2 cut(s) 781, 861
NgoMIV GCCGGC 1 cut(s) 74
NlaIII CATG 5 cut(s) 91, 395, 611, 764, 789
NlaIV GGNNCC 3 cut(s) 285, 602, 630
NmuCI GTSAC 1 cut(s) 336
PaqCI CACCTGC 1 cut(s) 170
PdiI GCCGGC 1 cut(s) 76
PfeI GAWTC 7 cut(s) 55, 423, 439, 573, 619, 673, 866
PkrI GCNGC 9 cut(s) 94, 115, 118, 148, 187, 190, 594, 721, 724
PleI GAGTC 3 cut(s) 116, 328, 694
PpsI GAGTC 3 cut(s) 116, 328, 694
Ppu21I YACGTR 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 463
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 3 cut(s) 285, 602, 630
PspPI GGNCC 1 cut(s) 284
PstNI CAGNNNCTG 1 cut(s) 622
PvuII CAGCTG 2 cut(s) 188, 592
RsaI GTAC 2 cut(s) 302, 630
RsaNI GTAC 2 cut(s) 301, 629
RseI CAYNNNNRTG 1 cut(s) 934
SalI GTCGAC 1 cut(s) 804
SaqAI TTAA 2 cut(s) 384, 773
SatI GCNGC 9 cut(s) 93, 114, 117, 147, 186, 189, 593, 720, 723
Sau3AI GATC 2 cut(s) 781, 861
Sau96I GGNCC 1 cut(s) 284
ScaI AGTACT 1 cut(s) 302
SchI GAGTC 3 cut(s) 117, 329, 694
ScrFI CCNGG 1 cut(s) 465
SduI GDGCHC 1 cut(s) 688
SfaNI GCATC 3 cut(s) 42, 873, 907
SfcI CTRYAG 1 cut(s) 837
SgrAI CRCCGGYG 1 cut(s) 74
SinI GGWCC 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 934
SnaBI TACGTA 1 cut(s) 712
Sse9I AATT 2 cut(s) 693, 774
SsiI CCGC 6 cut(s) 92, 114, 117, 147, 169, 731
SspI AATATT 1 cut(s) 418
SspMI CTAG 2 cut(s) 192, 341
StyD4I CCNGG 1 cut(s) 463
StyI CCWWGG 2 cut(s) 760, 785
TaaI ACNGT 3 cut(s) 300, 556, 796
TaiI ACGT 1 cut(s) 714
TaqI TCGA 2 cut(s) 805, 864
TasI AATT 2 cut(s) 693, 774
TatI WGTACW 1 cut(s) 300
TauI GCSGC 4 cut(s) 95, 116, 119, 149
TfiI GAWTC 7 cut(s) 55, 423, 439, 573, 619, 673, 866
Tru1I TTAA 2 cut(s) 384, 773
Tru9I TTAA 2 cut(s) 384, 773
TscAI CASTG 1 cut(s) 876
TseFI GTSAC 1 cut(s) 336
TseI GCWGC 5 cut(s) 185, 188, 592, 719, 722
Tsp45I GTSAC 1 cut(s) 336
TspRI CASTG 1 cut(s) 876
VpaK11BI GGWCC 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 805
XspI CTAG 2 cut(s) 192, 341
ZrmI AGTACT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.