Prupe.2G221900_v2.0.a1

Nitrilase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0015452
Physical Location & Seq
Forward (+)
24971468 .. 24974462
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G221900.1

Sequence Viewer

Length: 960 bp
ATGCCGTTTTGTCTGTCCTTACCTAACTCTGGTATCAGTCCACTCCTCCGACACGCCCCTCCGATTCGTTGCCAGTCCGCACGTGTCATCATCGCCGGCGAGTCAAACATGGCCGCCAACTCACTCCGAGTCGCCGCCGCTCAGATGACCTCTATCAATGACCTCGCCGCCAACTTCGCCACCTGCTCTCGCCTCGTCAAAGAAGCAGCTGCCGCTGGAGCAAAATTGATATGCTTTCCAGAAAGCTTCTCTTTTATTGGTGCCAAAGATGGGGACAGTCTCAAAATAGCACAACCTTTGGACGGTCCAATTTTGCAACAGTACTGCTCTCTAGCAAGAGAATCTGGGATTTGGTTGTCTCTTGGAGGGTTCCAAGAAAAGGGGTCTGATGATGAACATTTGTTTAACACCCATGTTGTGGTTGATGATGCTGGGAACATCAGAAGCACATACAGAAAGATTCACTTGTTTGATGTGGATATTCCTGGTGGAAGGGCATACAACGAAGGCAGCTTCACAGAACCAGGGAAGAATGTTGTTGCAGCAGATAGCCCGGTTGGACGCTTGGGTTTGACGGTTTGCTATGACCTGAGATTCCCAGAGCTTTACCAGCAGCTCAGGTTCCAACATGAGGCACAGGTTCTGTTGGTCCCTGCAGCCTTCACAAAAGTAACTGGGCTGGCGCACTGGGAGATTCTTCTACGTGCTCGTGCAATTGAAACTCAATGCTATGTCATAGCTGCAGCACAAGCTGGACAACATAATGATAAAAGAGAGAGCCACGGCGAAACTTTAATTATTGATCCATGGGGAACAGTTGTTGGCAGACTGCCTGATCGACTGTCAACAGGGATTGCTATAGCTGATATTGATCTCTCCGTGATCGATTCAGTGAGAGAAAAGATGCCAATTGCCAAGCACCGGAAGCCCGTTGAGTTCTGGAAATCTGCATCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

320

Amino Acids

34.7

Weight (kDa)

6.51

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 191
Acc36I ACCTGC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 418
AccBSI CCGCTC 1 cut(s) 140
AciI CCGC 6 cut(s) 78, 114, 135, 138, 168, 213
AclWI GGATC 1 cut(s) 797
AcoI YGGCCR 1 cut(s) 111
AcvI CACGTG 1 cut(s) 83
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 7 cut(s) 29, 270, 302, 379, 418, 631, 921
AflIII ACRYGT 1 cut(s) 82
AgsI TTSAA 1 cut(s) 719
AjnI CCWGG 2 cut(s) 484, 523
AluBI AGCT 8 cut(s) 209, 246, 513, 604, 616, 740, 752, 863
AluI AGCT 8 cut(s) 209, 246, 513, 604, 616, 740, 752, 863
Alw21I GWGCWC 1 cut(s) 709
Alw26I GTCTC 2 cut(s) 284, 363
AlwI GGATC 1 cut(s) 797
AlwNI CAGNNNCTG 1 cut(s) 643
AoxI GGCC 1 cut(s) 111
ApeKI GCWGC 8 cut(s) 206, 209, 510, 542, 613, 656, 740, 743
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 2 cut(s) 305, 649
AsuC2I CCSGG 1 cut(s) 554
AvaII GGWCC 2 cut(s) 305, 649
BaeI ACNNNNGTAYC 2 cut(s) 16, 49
BanI GGYRCC 1 cut(s) 260
BauI CACGAG 1 cut(s) 708
BbrPI CACGTG 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 709
BbvI GCAGC 8 cut(s) 196, 218, 522, 554, 625, 668, 727, 755
BccI CCATC 1 cut(s) 263
BceAI ACGGC 1 cut(s) 799
BciT130I CCWGG 2 cut(s) 486, 525
BcnI CCSGG 1 cut(s) 554
BcoDI GTCTC 2 cut(s) 284, 363
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 3 cut(s) 654, 741, 858
BfuAI ACCTGC 1 cut(s) 191
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 3 cut(s) 486, 525, 554
Bme18I GGWCC 2 cut(s) 305, 649
BmgT120I GGNCC 2 cut(s) 305, 649
BmiI GGNNCC 4 cut(s) 262, 371, 623, 651
BmrFI CCNGG 3 cut(s) 486, 525, 554
BmrI ACTGGG 2 cut(s) 684, 697
BmsI GCATC 2 cut(s) 418, 894
BmuI ACTGGG 2 cut(s) 684, 697
BpmI CTGGAG 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 617
BpuMI CCSGG 1 cut(s) 554
Bsa29I ATCGAT 1 cut(s) 885
BsaAI YACGTR 2 cut(s) 83, 704
BsaBI GATNNNNATC 1 cut(s) 870
BsaJI CCNNGG 3 cut(s) 524, 781, 806
BsaWI WCCGGW 1 cut(s) 921
Bsc4I CCNNNNNNNGG 7 cut(s) 29, 270, 302, 379, 418, 631, 921
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 3 cut(s) 73, 679, 692
Bse8I GATNNNNATC 1 cut(s) 870
BseBI CCWGG 2 cut(s) 486, 525
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 3 cut(s) 524, 781, 806
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 7 cut(s) 29, 270, 302, 379, 418, 631, 921
BseMII CTCAG 3 cut(s) 155, 581, 631
BseNI ACTGG 3 cut(s) 73, 679, 692
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 8 cut(s) 196, 218, 522, 554, 625, 668, 727, 755
BseYI CCCAGC 1 cut(s) 431
BshFI GGCC 1 cut(s) 113
BshNI GGYRCC 1 cut(s) 260
BshVI ATCGAT 1 cut(s) 885
BsiHKAI GWGCWC 1 cut(s) 709
BsiSI CCGG 3 cut(s) 96, 554, 922
BslFI GGGAC 2 cut(s) 287, 635
BslI CCNNNNNNNGG 7 cut(s) 29, 270, 302, 379, 418, 631, 921
BsmAI GTCTC 2 cut(s) 284, 363
BsmFI GGGAC 2 cut(s) 287, 635
BsnI GGCC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 709
Bsp143I GATC 4 cut(s) 802, 835, 871, 882
Bsp19I CCATGG 1 cut(s) 806
BspACI CCGC 6 cut(s) 78, 114, 135, 138, 168, 213
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 3 cut(s) 154, 582, 630
BspDI ATCGAT 1 cut(s) 885
BspLI GGNNCC 4 cut(s) 262, 371, 623, 651
BspMAI CTGCAG 2 cut(s) 658, 745
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 1 cut(s) 797
BspT107I GGYRCC 1 cut(s) 260
BsrBI CCGCTC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 3 cut(s) 73, 679, 692
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 524, 781, 806
BssMI GATC 4 cut(s) 802, 835, 871, 882
BssSI CACGAG 1 cut(s) 708
BssT1I CCWWGG 1 cut(s) 806
Bst2BI CACGAG 1 cut(s) 708
Bst2UI CCWGG 2 cut(s) 486, 525
Bst4CI ACNGT 6 cut(s) 278, 305, 321, 577, 817, 843
BstBAI YACGTR 2 cut(s) 83, 704
BstC8I GCNNGC 2 cut(s) 97, 681
BstDEI CTNAG 3 cut(s) 141, 590, 617
BstDSI CCRYGG 2 cut(s) 781, 806
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 4 cut(s) 805, 838, 874, 885
BstMAI GTCTC 2 cut(s) 284, 363
BstMBI GATC 4 cut(s) 802, 835, 871, 882
BstMWI GCNNNNNNNGC 6 cut(s) 176, 212, 218, 610, 749, 925
BstNI CCWGG 2 cut(s) 486, 525
BstSCI CCNGG 3 cut(s) 484, 523, 552
BstSFI CTRYAG 3 cut(s) 654, 741, 858
BstV1I GCAGC 8 cut(s) 196, 218, 522, 554, 625, 668, 727, 755
Bsu15I ATCGAT 1 cut(s) 885
BsuRI GGCC 1 cut(s) 113
BsuTUI ATCGAT 1 cut(s) 885
BtgI CCRYGG 2 cut(s) 781, 806
BtgZI GCGATG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 685, 897
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 2 cut(s) 97, 681
CaiI CAGNNNCTG 1 cut(s) 643
CfoI GCGC 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 2 cut(s) 305, 649
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 1 cut(s) 570
Csp6I GTAC 1 cut(s) 322
CviAII CATG 4 cut(s) 109, 413, 629, 807
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 3 cut(s) 141, 590, 617
DpnI GATC 4 cut(s) 804, 837, 873, 884
DpnII GATC 4 cut(s) 802, 835, 871, 882
EaeI YGGCCR 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 806
Eco47I GGWCC 2 cut(s) 305, 649
Eco72I CACGTG 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 484, 523
EcoT14I CCWWGG 1 cut(s) 806
ErhI CCWWGG 1 cut(s) 806
FaeI CATG 4 cut(s) 112, 416, 632, 810
FalI AAGNNNNNCTT 4 cut(s) 235, 267, 449, 481
FaqI GGGAC 2 cut(s) 287, 635
FatI CATG 4 cut(s) 108, 412, 628, 806
FspBI CTAG 1 cut(s) 332
GlaI GCGC 1 cut(s) 684
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 1 cut(s) 113
HapII CCGG 3 cut(s) 96, 554, 922
HgaI GACGC 1 cut(s) 570
HhaI GCGC 1 cut(s) 685
Hin1II CATG 4 cut(s) 112, 416, 632, 810
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HincII GTYRAC 1 cut(s) 846
HindII GTYRAC 1 cut(s) 846
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 8 cut(s) 64, 101, 129, 341, 460, 594, 694, 887
HpaII CCGG 3 cut(s) 96, 554, 922
Hpy166II GTNNAC 2 cut(s) 41, 846
Hpy188I TCNGA 6 cut(s) 50, 63, 128, 144, 388, 443
Hpy188III TCNNGA 2 cut(s) 239, 940
Hpy8I GTNNAC 2 cut(s) 41, 846
HpyAV CCTTC 3 cut(s) 486, 500, 670
HpyCH4III ACNGT 6 cut(s) 278, 305, 321, 577, 817, 843
HpyCH4IV ACGT 2 cut(s) 82, 703
HpyCH4V TGCA 6 cut(s) 316, 542, 656, 713, 743, 950
HpyF10VI GCNNNNNNNGC 6 cut(s) 176, 212, 218, 610, 749, 925
HpyF3I CTNAG 3 cut(s) 141, 590, 617
HpySE526I ACGT 2 cut(s) 82, 703
Hsp92II CATG 4 cut(s) 112, 416, 632, 810
HspAI GCGC 1 cut(s) 683
KroI GCCGGC 1 cut(s) 95
KroNI GCCGGC 1 cut(s) 97
Kzo9I GATC 4 cut(s) 802, 835, 871, 882
LmnI GCTCC 1 cut(s) 218
Lsp1109I GCAGC 8 cut(s) 196, 218, 522, 554, 625, 668, 727, 755
LweI GCATC 2 cut(s) 418, 894
MaeI CTAG 1 cut(s) 332
MaeII ACGT 2 cut(s) 82, 703
MaeIII GTNAC 1 cut(s) 670
MalI GATC 4 cut(s) 804, 837, 873, 884
MbiI CCGCTC 1 cut(s) 140
MboI GATC 4 cut(s) 802, 835, 871, 882
MboII GAAGA 2 cut(s) 541, 689
MfeI CAATTG 2 cut(s) 714, 909
MhlI GDGCHC 1 cut(s) 709
MluCI AATT 5 cut(s) 224, 309, 714, 795, 909
MlyI GAGTC 2 cut(s) 110, 138
MmeI TCCRAC 3 cut(s) 73, 538, 649
MnlI CCTC 7 cut(s) 56, 69, 160, 173, 203, 359, 625
MreI CGCCGGCG 1 cut(s) 95
MroNI GCCGGC 1 cut(s) 95
MseI TTAA 2 cut(s) 405, 794
MslI CAYNNNNRTG 1 cut(s) 955
MspA1I CMGCKG 2 cut(s) 209, 215
MspI CCGG 3 cut(s) 96, 554, 922
MspR9I CCNGG 3 cut(s) 486, 525, 554
MunI CAATTG 2 cut(s) 714, 909
MvaI CCWGG 2 cut(s) 486, 525
MwoI GCNNNNNNNGC 6 cut(s) 176, 212, 218, 610, 749, 925
NaeI GCCGGC 1 cut(s) 97
NciI CCSGG 1 cut(s) 554
NcoI CCATGG 1 cut(s) 806
NdeII GATC 4 cut(s) 802, 835, 871, 882
NgoMIV GCCGGC 1 cut(s) 95
NlaIII CATG 4 cut(s) 112, 416, 632, 810
NlaIV GGNNCC 4 cut(s) 262, 371, 623, 651
PaqCI CACCTGC 1 cut(s) 191
PdiI GCCGGC 1 cut(s) 97
PfeI GAWTC 6 cut(s) 64, 341, 460, 594, 694, 887
PflMI CCANNNNNTGG 1 cut(s) 418
PleI GAGTC 2 cut(s) 109, 137
PmaCI CACGTG 1 cut(s) 83
PmlI CACGTG 1 cut(s) 83
PpsI GAGTC 2 cut(s) 109, 137
Ppu21I YACGTR 2 cut(s) 83, 704
Psp6I CCWGG 2 cut(s) 484, 523
PspCI CACGTG 1 cut(s) 83
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 2 cut(s) 484, 523
PspN4I GGNNCC 4 cut(s) 262, 371, 623, 651
PspPI GGNCC 2 cut(s) 305, 649
PstI CTGCAG 2 cut(s) 658, 745
PstNI CAGNNNCTG 1 cut(s) 643
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 955
SaqAI TTAA 2 cut(s) 405, 794
Sau3AI GATC 4 cut(s) 802, 835, 871, 882
Sau96I GGNCC 2 cut(s) 305, 649
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 2 cut(s) 110, 138
ScrFI CCNGG 3 cut(s) 486, 525, 554
SduI GDGCHC 1 cut(s) 709
SfaNI GCATC 2 cut(s) 418, 894
SfcI CTRYAG 3 cut(s) 654, 741, 858
SgrAI CRCCGGYG 1 cut(s) 95
SinI GGWCC 2 cut(s) 305, 649
SmiMI CAYNNNNRTG 1 cut(s) 955
Sse9I AATT 5 cut(s) 224, 309, 714, 795, 909
SsiI CCGC 6 cut(s) 78, 114, 135, 138, 168, 213
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 3 cut(s) 484, 523, 552
StyI CCWWGG 1 cut(s) 806
TaaI ACNGT 6 cut(s) 278, 305, 321, 577, 817, 843
TaiI ACGT 2 cut(s) 85, 706
TaqI TCGA 2 cut(s) 838, 885
TasI AATT 5 cut(s) 224, 309, 714, 795, 909
TatI WGTACW 1 cut(s) 321
TauI GCSGC 5 cut(s) 116, 137, 140, 170, 215
TfiI GAWTC 6 cut(s) 64, 341, 460, 594, 694, 887
Tru1I TTAA 2 cut(s) 405, 794
Tru9I TTAA 2 cut(s) 405, 794
TscAI CASTG 2 cut(s) 692, 897
TseI GCWGC 8 cut(s) 206, 209, 510, 542, 613, 656, 740, 743
TspDTI ATGAA 1 cut(s) 408
TspGWI ACGGA 1 cut(s) 868
TspRI CASTG 2 cut(s) 692, 897
Van91I CCANNNNNTGG 1 cut(s) 418
VpaK11BI GGWCC 2 cut(s) 305, 649
XspI CTAG 1 cut(s) 332
ZrmI AGTACT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.