Rmu_sc0019872.1_g000001

Nitrilase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0019872.1
OG0015452
Physical Location & Seq
Forward (+)
1 .. 1820
1820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0019872.1_g000001.1.cds

Sequence Viewer

Length: 585 bp
gaaaaggggtctgatgatgagcaattgtttaacacccatgttgtggttgatgatgctggcaacatcagaagcacttacagaaagattcacttgtttgatgtggatactcctggtgggaggtcatacaaggaaagtagctttacagaagcagggaagaatgttgttgcagtagatagccctgtgggacgtttgggtttgacagtttgctatgacctgagattcccagagatttaccagctgctcaggttccaacatgaggcacagattctgttagtaccttcagcctttaccaaagttactggtcaggcacattgggaggttcttcttcgtgctcgtgcaattgagactcaatgctatgtaatagcttctgcacaagctggaaagcataatgataaaagacagagccatggcgaaactttaattattgatccatggggaactgttgttggtcgactacctgatactttgtcaaccgggattactatagctgatattgatttctcattgatcgattcagtgagagcaaagatgccaattgccaagcatcggaaacctgttgagttctggaaatctgcatctctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CN_hydrolase

Protein Analysis

194

Amino Acids

21.62

Weight (kDa)

6.74

Isoelectric Point (pI)

36.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08790
fragaria_vesca FvH4_7g20650
malus_domestica MD07G1183200.v1.1
prunus_persica Prupe.2G221900_v2.0.a1
pyrus_communis pycom07g17580
rosa_chinensis RchiOBHm_Chr1g0364681
rosa_laevigata RLG00000027467
rosa_multiflora Rmu_sc0019872.1_g000001 Rmu_ssc0000112.1_g000034
rosa_roxburghii Rroxscaffold_4G00291750
rosa_rugosa Rorug01G0317700.1
rosa_samantha Rh1AG326700 Rh1BG287900 Rh1CG304700 Rh1DG319600
rosa_wichuraiana Rw1G028940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 43
AccI GTMKAC 1 cut(s) 451
AclWI GGATC 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 264
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 43, 256, 546
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 5 cut(s) 138, 238, 365, 377, 488
AluI AGCT 5 cut(s) 138, 238, 365, 377, 488
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 1 cut(s) 338
AlwI GGATC 1 cut(s) 422
AlwNI CAGNNNCTG 1 cut(s) 268
ApeKI GCWGC 1 cut(s) 238
AsuC2I CCSGG 1 cut(s) 475
BauI CACGAG 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 1 cut(s) 225
BciT130I CCWGG 1 cut(s) 111
BciVI GTATCC 1 cut(s) 97
BcnI CCSGG 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 483
BfuI GTATCC 1 cut(s) 97
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 2 cut(s) 111, 475
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 2 cut(s) 111, 475
BmsI GCATC 3 cut(s) 43, 519, 553
Bpu10I CCTNAGC 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 475
Bsa29I ATCGAT 1 cut(s) 510
BsaJI CCNNGG 2 cut(s) 406, 431
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 256, 546
Bse1I ACTGG 1 cut(s) 304
BseBI CCWGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 406, 431
BseLI CCNNNNNNNGG 3 cut(s) 43, 256, 546
BseMII CTCAG 2 cut(s) 206, 256
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 225
BsgI GTGCAG 1 cut(s) 354
BshVI ATCGAT 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 1 cut(s) 474
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 3 cut(s) 43, 256, 546
BsmAI GTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 2 cut(s) 427, 507
Bsp19I CCATGG 2 cut(s) 406, 431
BspCNI CTCAG 2 cut(s) 207, 255
BspDI ATCGAT 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 248
BspPI GGATC 1 cut(s) 422
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 406, 431
BssMI GATC 2 cut(s) 427, 507
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 406, 431
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 202, 442
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 2 cut(s) 215, 242
BstDSI CCRYGG 2 cut(s) 406, 431
BstKTI GATC 2 cut(s) 430, 510
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 2 cut(s) 427, 507
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 2 cut(s) 109, 473
BstSFI CTRYAG 1 cut(s) 483
BstV1I GCAGC 1 cut(s) 225
Bsu15I ATCGAT 1 cut(s) 510
BsuI GTATCC 1 cut(s) 97
BsuTUI ATCGAT 1 cut(s) 510
BtgI CCRYGG 2 cut(s) 406, 431
BtsIMutI CAGTG 1 cut(s) 522
Cac8I GCNNGC 1 cut(s) 58
CaiI CAGNNNCTG 1 cut(s) 268
ClaI ATCGAT 1 cut(s) 510
Csp6I GTAC 1 cut(s) 275
CviAII CATG 4 cut(s) 38, 254, 407, 432
CviJI RGCY 8 cut(s) 138, 177, 238, 284, 365, 377, 405, 488
CviKI_1 RGCY 8 cut(s) 138, 177, 238, 284, 365, 377, 405, 488
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 2 cut(s) 215, 242
DpnI GATC 2 cut(s) 429, 509
DpnII GATC 2 cut(s) 427, 507
Eco130I CCWWGG 2 cut(s) 406, 431
Eco57I CTGAAG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 406, 431
ErhI CCWWGG 2 cut(s) 406, 431
FaeI CATG 4 cut(s) 41, 257, 410, 435
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 198
FatI CATG 4 cut(s) 37, 253, 406, 431
FblI GTMKAC 1 cut(s) 451
Fnu4HI GCNGC 1 cut(s) 239
Fsp4HI GCNGC 1 cut(s) 239
GluI GCNGC 1 cut(s) 239
HapII CCGG 1 cut(s) 474
Hin1II CATG 4 cut(s) 41, 257, 410, 435
HincII GTYRAC 2 cut(s) 452, 471
HindII GTYRAC 2 cut(s) 452, 471
HinfI GANTC 5 cut(s) 85, 219, 265, 346, 512
HpaII CCGG 1 cut(s) 474
Hpy166II GTNNAC 2 cut(s) 452, 471
Hpy188I TCNGA 3 cut(s) 13, 68, 549
Hpy188III TCNNGA 1 cut(s) 565
Hpy8I GTNNAC 2 cut(s) 452, 471
HpyAV CCTTC 1 cut(s) 288
HpyCH4III ACNGT 2 cut(s) 202, 442
HpyCH4IV ACGT 1 cut(s) 187
HpyCH4V TGCA 4 cut(s) 167, 338, 371, 575
HpyF3I CTNAG 2 cut(s) 215, 242
HpySE526I ACGT 1 cut(s) 187
Hsp92II CATG 4 cut(s) 41, 257, 410, 435
Kzo9I GATC 2 cut(s) 427, 507
Lsp1109I GCAGC 1 cut(s) 225
LweI GCATC 3 cut(s) 43, 519, 553
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 1 cut(s) 295
MalI GATC 2 cut(s) 429, 509
MboI GATC 2 cut(s) 427, 507
MboII GAAGA 3 cut(s) 166, 314, 317
MfeI CAATTG 3 cut(s) 23, 339, 534
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 4 cut(s) 23, 339, 420, 534
MlyI GAGTC 1 cut(s) 340
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 3 cut(s) 111, 250, 310
MseI TTAA 2 cut(s) 30, 419
MslI CAYNNNNRTG 1 cut(s) 580
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 1 cut(s) 474
MspR9I CCNGG 2 cut(s) 111, 475
MunI CAATTG 3 cut(s) 23, 339, 534
MvaI CCWGG 1 cut(s) 111
NciI CCSGG 1 cut(s) 475
NcoI CCATGG 2 cut(s) 406, 431
NdeII GATC 2 cut(s) 427, 507
NlaIII CATG 4 cut(s) 41, 257, 410, 435
NlaIV GGNNCC 1 cut(s) 248
PfeI GAWTC 4 cut(s) 85, 219, 265, 512
PflMI CCANNNNNTGG 1 cut(s) 43
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
RseI CAYNNNNRTG 1 cut(s) 580
SalI GTCGAC 1 cut(s) 450
SaqAI TTAA 2 cut(s) 30, 419
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 2 cut(s) 427, 507
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 2 cut(s) 111, 475
SduI GDGCHC 1 cut(s) 334
SfaNI GCATC 3 cut(s) 43, 519, 553
SfcI CTRYAG 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 580
Sse9I AATT 4 cut(s) 23, 339, 420, 534
StyD4I CCNGG 2 cut(s) 109, 473
StyI CCWWGG 2 cut(s) 406, 431
TaaI ACNGT 2 cut(s) 202, 442
TaiI ACGT 1 cut(s) 190
TaqI TCGA 2 cut(s) 451, 510
TasI AATT 4 cut(s) 23, 339, 420, 534
TfiI GAWTC 4 cut(s) 85, 219, 265, 512
Tru1I TTAA 2 cut(s) 30, 419
Tru9I TTAA 2 cut(s) 30, 419
TscAI CASTG 1 cut(s) 522
TseI GCWGC 1 cut(s) 238
TspRI CASTG 1 cut(s) 522
Van91I CCANNNNNTGG 1 cut(s) 43
XmiI GTMKAC 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.