AT4G08840

Pumilio homolog 11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009606
Physical Location & Seq
Forward (+)
5631298 .. 5633779
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08840.1

Sequence Viewer

Length: 1671 bp
ATGGATTTTGGGTTTTTTCCTGGTGATTTGAGACAACGAGGTTCATTTACTGATCTGGGTTTTAATGGGTTTCCTCTAACTTCCTCTGTCTCTAATGGATTTCACTTTTCCGGTGACCGTACAACAAACCCTTTTCTTAACCTTCGAAAGCTTGATACGACGTCGTTAATGGCGGATGGTGTTGATATGGGTTTGTGTCAAAACCTCTCTAAAATGAGTATCTCCGATGAAAGAAGCAATTTTTTTAACCACTCTAGTTTTTCCGGCTATGGATGTTACCAAGGTCGTGAGAGCTCCTTTCATGGTGAAGCTTCTAGCTCTATGCGTGGTTTTGTTGGTTATGGTGATGTTCATAGATTCGAACAAGATCTGAGAGTAAGAGCGAGCTTTCATGGTGAATCTGCTATGAGTAGTTATGTTGGTGATGGTAGTGATTATCATAGACTCAGATTTCTTGCCTTACAAGAAGCTTCTAACCCTAACCCTAGATGTTTTACAGAGAACATGTCTCTTCTTAACAGAGATTACATGTTGGAGTTGGAGCATTTCAATCAACAAATCCGGCGAGACTTTTCTCTAGTTCCACAGAAATCTCCACTAGCTTTTCATGAAGAGAGGATCTTGCCACCATTTTCAGCCATGGGAGGTTCTAGAGAGCTTGATGGTTCTGCTAAATGTATGAAGAACAAGGAAGACTCACTAGATCTTGCTTCAATGGTTGATAGCTATGGTTCTGTGTACTTAATGGCAAAAGATCAACTGGGTTGTCGATTATTACAGAAGTTTGTAGACGAAGGAAATTTTGTTGATGTGATGATTATATTCAAGGAAGTGATTAATAATGTCATTGAGCTTGGAACGGATCCGTTTGGTAACTATCTTATCCAGAAGCTTATTGAAGTCTGCAATGAAGAACAAAGAACTCAGATTCTCATTAGGTTGACTTCAAAACCTGGACTTCTTGTCAAAATCTCCATCAACAATTATGGGACACGAGTGGTGCAGAAGTTGATTGAGACTGTGACTACAAAGGAGCAAATCAGTCTGGTTAAGTCAGCTCTTGTACCGGGATTTCTCTCTTTGTTTAGAGAATTAAATGGAAACCATGTGATACTAAATTGCTTGAAATTCTTTAGTCCTAATGATAACAAGTTCATTTTGGAGGCTGCTACTAAGTTCTGCATAGAGATTGCGACTACTCGCCATGGATGTTGTGTGCTTCAACGCTGCGTTTCGTACTCGGTTGGGGAGCAACATGAGAAGCTTGTTGATGAAATATCCAGAAACTCTCTTCTCCTTGCTCAAGACCCTTTCGGGAACTATTTGGTGCAATACATAATAGAGAAGAAGGTTGGAGGAGTGAATGTACTGTTTGAGCTAAGAGGGAATTATGTGAAGTTAGCTACACAGAAGTTTGGTAGCCATGTGGTTGAGAAATGTCTCAGGTATTATCCAGAGAGCAGATCTCAGATTGTGAATGAACTCGTCTCTGTTCTAAACTTCGGATATCTTCTTCAAGACCCTTATGCTAATTACGTGATCCAGTGTGCTCTATCGAAAACCAAGGGCTTTGTTCGAGCGAGCTTGGTGGAGAAGGTTAGAAGATATGAAAATCTGAAGATGACTCCATATTGCAAGAGGATATTCTCCAAGAATCTCTGGAAGAAGTGA

Protein Analysis

556

Amino Acids

63.13

Weight (kDa)

8.4

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 246 - 279 2.6e-07 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 248 - 496 6.9e-11 NOP9-like PUF repeat domain
PUF PF00806 281 - 311 1.1e-10 Pumilio-family RNA binding repeat
PUF PF00806 394 - 426 3.6e-10 Pumilio-family RNA binding repeat
PUF PF00806 463 - 495 1.4e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 789
AciI CCGC 1 cut(s) 173
AclWI GGATC 4 cut(s) 626, 857, 870, 1534
AcsI RAATTY 2 cut(s) 799, 1127
AcuI CTGAAG 1 cut(s) 1637
AcyI GRCGYC 1 cut(s) 161
AfaI GTAC 5 cut(s) 121, 740, 1065, 1238, 1368
AflIII ACRYGT 2 cut(s) 504, 528
AgsI TTSAA 8 cut(s) 550, 714, 826, 899, 948, 1126, 1223, 1517
AhdI GACNNNNNGTC 1 cut(s) 962
AjnI CCWGG 2 cut(s) 19, 952
Alw21I GWGCWC 2 cut(s) 296, 1552
Alw26I GTCTC 7 cut(s) 25, 94, 513, 561, 1010, 1445, 1492
AlwI GGATC 4 cut(s) 626, 857, 870, 1534
ApeKI GCWGC 2 cut(s) 1166, 1227
ApoI RAATTY 2 cut(s) 799, 1127
AseI ATTAAT 1 cut(s) 837
AsuC2I CCSGG 1 cut(s) 1068
AsuHPI GGTGA 6 cut(s) 35, 125, 317, 356, 407, 434
AsuII TTCGAA 2 cut(s) 145, 360
BamHI GGATCC 1 cut(s) 862
BanII GRGCYC 1 cut(s) 296
BauI CACGAG 1 cut(s) 993
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 2 cut(s) 296, 1552
BbvI GCAGC 2 cut(s) 1153, 1214
BccI CCATC 4 cut(s) 170, 419, 656, 983
BciT130I CCWGG 2 cut(s) 21, 954
BcnI CCSGG 1 cut(s) 1068
BcoDI GTCTC 7 cut(s) 25, 94, 513, 561, 1010, 1445, 1492
BfaI CTAG 7 cut(s) 255, 315, 486, 578, 599, 651, 701
BglII AGATCT 3 cut(s) 367, 703, 1463
BisI GCNGC 2 cut(s) 1167, 1228
BlsI GCNGC 2 cut(s) 1168, 1229
Bme1390I CCNGG 3 cut(s) 21, 954, 1068
BmeRI GACNNNNNGTC 1 cut(s) 962
BmiI GGNNCC 1 cut(s) 864
BmrFI CCNGG 3 cut(s) 21, 954, 1068
BmrI ACTGGG 1 cut(s) 770
BmuI ACTGGG 1 cut(s) 770
BpiI GAAGAC 1 cut(s) 699
BplI GAGNNNNNCTC 2 cut(s) 1450, 1482
Bpu14I TTCGAA 2 cut(s) 145, 360
BpuEI CTTGAG 1 cut(s) 1287
BpuMI CCSGG 1 cut(s) 1068
BsaAI YACGTR 1 cut(s) 1537
BsaHI GRCGYC 1 cut(s) 161
BsaJI CCNNGG 4 cut(s) 280, 639, 1204, 1563
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 1350, 1380
Bse1I ACTGG 2 cut(s) 765, 1543
Bse3DI GCAATG 1 cut(s) 913
BseBI CCWGG 2 cut(s) 21, 954
BseDI CCNNGG 4 cut(s) 280, 639, 1204, 1563
BseGI GGATG 3 cut(s) 181, 278, 1214
BseMI GCAATG 1 cut(s) 913
BseMII CTCAG 5 cut(s) 362, 460, 938, 1456, 1481
BseNI ACTGG 2 cut(s) 765, 1543
BseRI GAGGAG 1 cut(s) 1371
BseXI GCAGC 2 cut(s) 1153, 1214
BsgI GTGCAG 1 cut(s) 1022
BsiHKAI GWGCWC 2 cut(s) 296, 1552
BsiSI CCGG 4 cut(s) 111, 264, 562, 1067
BslFI GGGAC 1 cut(s) 1003
BsmAI GTCTC 7 cut(s) 25, 94, 513, 561, 1010, 1445, 1492
BsmBI CGTCTC 1 cut(s) 1492
BsmFI GGGAC 1 cut(s) 1003
Bsp119I TTCGAA 2 cut(s) 145, 360
Bsp1286I GDGCHC 2 cut(s) 296, 1552
Bsp143I GATC 8 cut(s) 52, 367, 618, 703, 754, 862, 1463, 1539
Bsp19I CCATGG 2 cut(s) 639, 1204
BspACI CCGC 1 cut(s) 173
BspCNI CTCAG 5 cut(s) 363, 459, 937, 1455, 1480
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 864
BspPI GGATC 4 cut(s) 626, 857, 870, 1534
BspT104I TTCGAA 2 cut(s) 145, 360
BsrDI GCAATG 1 cut(s) 913
BsrI ACTGG 2 cut(s) 765, 1543
BssECI CCNNGG 4 cut(s) 280, 639, 1204, 1563
BssMI GATC 8 cut(s) 52, 367, 618, 703, 754, 862, 1463, 1539
BssNI GRCGYC 1 cut(s) 161
BssSI CACGAG 1 cut(s) 993
BssT1I CCWWGG 4 cut(s) 280, 639, 1204, 1563
Bst2BI CACGAG 1 cut(s) 993
Bst2UI CCWGG 2 cut(s) 21, 954
Bst4CI ACNGT 3 cut(s) 119, 1021, 1371
Bst6I CTCTTC 3 cut(s) 516, 606, 1296
BstACI GRCGYC 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 1537
BstBI TTCGAA 2 cut(s) 145, 360
BstC8I GCNNGC 2 cut(s) 385, 1582
BstDEI CTNAG 7 cut(s) 371, 446, 924, 1173, 1379, 1442, 1467
BstDSI CCRYGG 2 cut(s) 639, 1204
BstEII GGTNACC 1 cut(s) 113
BstF5I GGATG 3 cut(s) 181, 278, 1214
BstKTI GATC 8 cut(s) 55, 370, 621, 706, 757, 865, 1466, 1542
BstMAI GTCTC 7 cut(s) 25, 94, 513, 561, 1010, 1445, 1492
BstMBI GATC 8 cut(s) 52, 367, 618, 703, 754, 862, 1463, 1539
BstNI CCWGG 2 cut(s) 21, 954
BstNSI RCATGY 2 cut(s) 508, 532
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 3 cut(s) 19, 952, 1066
BstV1I GCAGC 2 cut(s) 1153, 1214
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 5 cut(s) 367, 618, 703, 862, 1463
BstYI RGATCY 5 cut(s) 367, 618, 703, 862, 1463
BtgI CCRYGG 2 cut(s) 639, 1204
BtsCI GGATG 3 cut(s) 181, 278, 1214
BtsIMutI CAGTG 1 cut(s) 1550
Cac8I GCNNGC 2 cut(s) 385, 1582
CciI TCATGA 1 cut(s) 607
Csp6I GTAC 5 cut(s) 120, 739, 1064, 1237, 1367
CviQI GTAC 5 cut(s) 120, 739, 1064, 1237, 1367
DdeI CTNAG 7 cut(s) 371, 446, 924, 1173, 1379, 1442, 1467
DpnI GATC 8 cut(s) 54, 369, 620, 705, 756, 864, 1465, 1541
DpnII GATC 8 cut(s) 52, 367, 618, 703, 754, 862, 1463, 1539
DriI GACNNNNNGTC 1 cut(s) 962
Eam1104I CTCTTC 3 cut(s) 516, 606, 1296
Eam1105I GACNNNNNGTC 1 cut(s) 962
EarI CTCTTC 3 cut(s) 516, 606, 1296
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 294
Eco130I CCWWGG 4 cut(s) 280, 639, 1204, 1563
Eco24I GRGCYC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 1508
Eco53kI GAGCTC 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 1637
Eco91I GGTNACC 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 294
EcoO65I GGTNACC 1 cut(s) 113
EcoRII CCWGG 2 cut(s) 19, 952
EcoRV GATATC 1 cut(s) 1508
EcoT14I CCWWGG 4 cut(s) 280, 639, 1204, 1563
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 4 cut(s) 280, 639, 1204, 1563
Esp3I CGTCTC 1 cut(s) 1492
FaqI GGGAC 1 cut(s) 1003
FblI GTMKAC 1 cut(s) 789
Fnu4HI GCNGC 2 cut(s) 1167, 1228
FokI GGATG 3 cut(s) 188, 285, 1221
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 1167, 1228
FspBI CTAG 7 cut(s) 255, 315, 486, 578, 599, 651, 701
GluI GCNGC 2 cut(s) 1167, 1228
HapII CCGG 4 cut(s) 111, 264, 562, 1067
Hin1I GRCGYC 1 cut(s) 161
HincII GTYRAC 1 cut(s) 942
HindII GTYRAC 1 cut(s) 942
HindIII AAGCTT 5 cut(s) 149, 309, 468, 890, 1262
HinfI GANTC 7 cut(s) 357, 398, 444, 695, 928, 1624, 1654
HpaII CCGG 4 cut(s) 111, 264, 562, 1067
HphI GGTGA 6 cut(s) 35, 125, 317, 356, 407, 434
Hpy166II GTNNAC 3 cut(s) 739, 790, 942
Hpy188I TCNGA 7 cut(s) 226, 372, 449, 927, 1470, 1505, 1617
Hpy8I GTNNAC 3 cut(s) 739, 790, 942
Hpy99I CGWCG 2 cut(s) 163, 166
HpyAV CCTTC 4 cut(s) 152, 788, 1342, 1588
HpyCH4III ACNGT 3 cut(s) 119, 1021, 1371
HpyCH4IV ACGT 2 cut(s) 161, 1536
HpyCH4V TGCA 5 cut(s) 906, 1003, 1182, 1330, 1635
HpyF3I CTNAG 7 cut(s) 371, 446, 924, 1173, 1379, 1442, 1467
HpySE526I ACGT 2 cut(s) 161, 1536
Hsp92I GRCGYC 1 cut(s) 161
Kzo9I GATC 8 cut(s) 52, 367, 618, 703, 754, 862, 1463, 1539
LmnI GCTCC 4 cut(s) 299, 541, 1033, 1249
Lsp1109I GCAGC 2 cut(s) 1153, 1214
MaeI CTAG 7 cut(s) 255, 315, 486, 578, 599, 651, 701
MaeII ACGT 2 cut(s) 161, 1536
MaeIII GTNAC 4 cut(s) 113, 275, 872, 1021
MalI GATC 8 cut(s) 54, 369, 620, 705, 756, 864, 1465, 1541
MboI GATC 8 cut(s) 52, 367, 618, 703, 754, 862, 1463, 1539
MflI RGATCY 5 cut(s) 367, 618, 703, 862, 1463
MhlI GDGCHC 2 cut(s) 296, 1552
MluCI AATT 8 cut(s) 238, 799, 982, 1091, 1117, 1127, 1387, 1531
MlyI GAGTC 3 cut(s) 438, 689, 1618
MmeI TCCRAC 3 cut(s) 513, 519, 1333
MseI TTAA 9 cut(s) 63, 138, 167, 246, 516, 743, 837, 1050, 1094
MspI CCGG 4 cut(s) 111, 264, 562, 1067
MspR9I CCNGG 3 cut(s) 21, 954, 1068
MvaI CCWGG 2 cut(s) 21, 954
NciI CCSGG 1 cut(s) 1068
NcoI CCATGG 2 cut(s) 639, 1204
NdeII GATC 8 cut(s) 52, 367, 618, 703, 754, 862, 1463, 1539
NlaIV GGNNCC 1 cut(s) 864
NmuCI GTSAC 2 cut(s) 113, 1021
NspI RCATGY 2 cut(s) 508, 532
NspV TTCGAA 2 cut(s) 145, 360
PagI TCATGA 1 cut(s) 607
PciI ACATGT 2 cut(s) 504, 528
PfeI GAWTC 4 cut(s) 357, 398, 928, 1654
PkrI GCNGC 2 cut(s) 1168, 1229
PleI GAGTC 3 cut(s) 438, 689, 1618
PpsI GAGTC 3 cut(s) 438, 689, 1618
Ppu21I YACGTR 1 cut(s) 1537
PscI ACATGT 2 cut(s) 504, 528
PshBI ATTAAT 1 cut(s) 837
Psp124BI GAGCTC 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 19, 952
PspEI GGTNACC 1 cut(s) 113
PspGI CCWGG 2 cut(s) 19, 952
PspN4I GGNNCC 1 cut(s) 864
PsuI RGATCY 5 cut(s) 367, 618, 703, 862, 1463
RsaI GTAC 5 cut(s) 121, 740, 1065, 1238, 1368
RsaNI GTAC 5 cut(s) 120, 739, 1064, 1237, 1367
SacI GAGCTC 1 cut(s) 296
SaqAI TTAA 9 cut(s) 63, 138, 167, 246, 516, 743, 837, 1050, 1094
SatI GCNGC 2 cut(s) 1167, 1228
Sau3AI GATC 8 cut(s) 52, 367, 618, 703, 754, 862, 1463, 1539
SchI GAGTC 3 cut(s) 438, 689, 1618
ScrFI CCNGG 3 cut(s) 21, 954, 1068
SduI GDGCHC 2 cut(s) 296, 1552
SfuI TTCGAA 2 cut(s) 145, 360
SmlI CTYRAG 1 cut(s) 1302
SmoI CTYRAG 1 cut(s) 1302
Sse9I AATT 8 cut(s) 238, 799, 982, 1091, 1117, 1127, 1387, 1531
SsiI CCGC 1 cut(s) 173
SspMI CTAG 7 cut(s) 255, 315, 486, 578, 599, 651, 701
SstI GAGCTC 1 cut(s) 296
StyD4I CCNGG 3 cut(s) 19, 952, 1066
StyI CCWWGG 4 cut(s) 280, 639, 1204, 1563
TaaI ACNGT 3 cut(s) 119, 1021, 1371
TaiI ACGT 2 cut(s) 164, 1539
TaqI TCGA 5 cut(s) 145, 360, 769, 1556, 1576
TasI AATT 8 cut(s) 238, 799, 982, 1091, 1117, 1127, 1387, 1531
TatI WGTACW 2 cut(s) 738, 1366
TfiI GAWTC 4 cut(s) 357, 398, 928, 1654
Tru1I TTAA 9 cut(s) 63, 138, 167, 246, 516, 743, 837, 1050, 1094
Tru9I TTAA 9 cut(s) 63, 138, 167, 246, 516, 743, 837, 1050, 1094
TscAI CASTG 1 cut(s) 1550
TseFI GTSAC 2 cut(s) 113, 1021
TseI GCWGC 2 cut(s) 1166, 1227
Tsp45I GTSAC 2 cut(s) 113, 1021
TspGWI ACGGA 2 cut(s) 855, 875
TspRI CASTG 1 cut(s) 1550
VspI ATTAAT 1 cut(s) 837
XapI RAATTY 2 cut(s) 799, 1127
XbaI TCTAGA 1 cut(s) 650
XceI RCATGY 2 cut(s) 508, 532
XmiI GTMKAC 1 cut(s) 789
XspI CTAG 7 cut(s) 255, 315, 486, 578, 599, 651, 701
ZraI GACGTC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.