Rh6DG483100

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009606
Physical Location & Seq
Forward (+)
64628719 .. 64632452
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG483100.1

Sequence Viewer

Length: 1314 bp
ATGCGAATTGAGACATTTAGAGAAAGAAGAACGCTGCGTCGCTTTTTCTCGCATGTAAATGTTTCCATAAATTGCATGCAAACTCAGACTCCCTCTCTCTCTCTCTCTCTCTTCCTCATCAGTCTCATCAGTCTTAAGCTTTTCTTCCCTGCCACACTCAACCAATACCAAAATTCCCAAAAAATCAAAACCCCCCTCATATATATATATAACTCCTCATTAAACCCAGAAACCAAACCCAACCAAACCTCAGAAACCCTTTCACACTATAATCCCCCTACTCGATCAGAAGCCAGTTTTCTCTTCGTTTCAGAAACAATGGAGGGTTTCCGATACTTTACCGATACGACGTCGCAGAACCCTATTCCCTATGATCAATCCCTAGAAGCTGCTTTCTCTCGCCTGAATGTCTCGTCCTTCAGCCAGCCGCCGCCGTTTTCCGGCGCCGATTACAGTTCTGGATCGGCGCCTAAATACCCATCACGCCGCAATGAACTCATGCCGGGTGGTTTTGATGGTGGGGTTTTCGGTCATTTGGGTCTTGAAGATTGCTGGGTTGGCTCAGATACTGATCATGGCTTAGGTGGGTTTGGTTCTAGTTTACATAAACCATCTGATTGGTCCCAAGAACCACTCAACAGCTTATCTGTGGCTGATTTGCGTGGAAAGATTGCTTCTTTGTCCATGGACCAATACGGGTGTCGGTTCTTTCAGAGAGCTATGGAGGATGAGCCGAAAGAGTTCACTGATATGATATTTGAAGAGGTTGTAGACCACGTGGCCCATTTGATCCTGGACCCTTATGGCAACTATGTTGTTCAGAAGCTTGTGGAGGTTTGTAGTGAGGAGCAGAGGACACAGATTCTGTTAAGGTGGACCAAGAATCCGTTTCATTTTATTACTCTTTGCCTTCATAGCCGTGGGACTCGCTCTGTTCTGAAACTGTTGAAGAAAATCACTACCCGAGAGCAAATCTCTGTGTTTGTGAGTGCTCTAAGCTGTGGTGCTGTTGACCTGAGTATGAACATCAATGGTCAACATATCATGGAATATTGCTTGAAGAACTTTTCGGATGAACACAAAAGGTGTCTTCTGAATGTGGTGATTGACAACTGTATTGGAATTGCGAAAGATAAGGTTGGTTGTTGCGTGCTACAGCAGTGTGTTGATTACTGTTATGGAGAAACAAAAGGACGTCTAATTGCTGCGATAATAGAAGAAGCATTAATCCTGGCGCAGGATCGTTATGGGTTTGTTCCTATGAAACTATATCAATACTTCTCTTGCAGTTTGATCATGACCTTTACTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

437

Amino Acids

49.53

Weight (kDa)

6.27

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 224 - 256 5.2e-08 Pumilio-family RNA binding repeat
PUF PF00806 259 - 289 1.6e-10 Pumilio-family RNA binding repeat
PUF PF00806 371 - 405 1.4e-09 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 353, 1198
AccB1I GGYRCC 2 cut(s) 443, 466
AccI GTMKAC 1 cut(s) 771
AciI CCGC 3 cut(s) 428, 431, 487
AclWI GGATC 3 cut(s) 469, 784, 1248
AcsI RAATTY 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 403
AcvI CACGTG 1 cut(s) 778
AcyI GRCGYC 4 cut(s) 350, 444, 467, 1195
AfiI CCNNNNNNNGG 2 cut(s) 440, 1237
AflII CTTAAG 1 cut(s) 134
AgsI TTSAA 4 cut(s) 545, 761, 949, 1060
AjnI CCWGG 2 cut(s) 792, 1230
AluBI AGCT 6 cut(s) 139, 389, 642, 719, 826, 999
AluI AGCT 6 cut(s) 139, 389, 642, 719, 826, 999
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 3 cut(s) 5, 128, 415
AlwI GGATC 3 cut(s) 469, 784, 1248
AlwNI CAGNNNCTG 2 cut(s) 569, 865
Ama87I CYCGRG 1 cut(s) 963
AoxI GGCC 1 cut(s) 780
ApeKI GCWGC 3 cut(s) 34, 389, 1205
ApoI RAATTY 1 cut(s) 172
AseI ATTAAT 1 cut(s) 1226
Asp700I GAANNNNTTC 1 cut(s) 740
AspLEI GCGC 3 cut(s) 446, 469, 1237
AspS9I GGNCC 5 cut(s) 621, 688, 781, 796, 876
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 1 cut(s) 1114
AvaI CYCGRG 1 cut(s) 963
AvaII GGWCC 4 cut(s) 621, 688, 796, 876
BanI GGYRCC 2 cut(s) 443, 466
BbrPI CACGTG 1 cut(s) 778
BbsI GAAGAC 1 cut(s) 1082
Bbv12I GWGCWC 1 cut(s) 994
BbvI GCAGC 3 cut(s) 21, 376, 1192
BccI CCATC 3 cut(s) 487, 509, 619
BceAI ACGGC 2 cut(s) 418, 903
BciT130I CCWGG 2 cut(s) 794, 1232
BclI TGATCA 3 cut(s) 373, 571, 1293
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 3 cut(s) 5, 128, 415
BfaI CTAG 2 cut(s) 383, 597
BfmI CTRYAG 1 cut(s) 1154
BfoI RGCGCY 2 cut(s) 447, 470
BfrI CTTAAG 1 cut(s) 134
BisI GCNGC 6 cut(s) 35, 390, 428, 431, 487, 1206
BlsI GCNGC 6 cut(s) 36, 391, 429, 432, 488, 1207
Bme1390I CCNGG 3 cut(s) 504, 794, 1232
Bme18I GGWCC 4 cut(s) 621, 688, 796, 876
BmeT110I CYCGRG 1 cut(s) 963
BmgT120I GGNCC 5 cut(s) 621, 688, 781, 796, 876
BmiI GGNNCC 4 cut(s) 445, 468, 623, 798
BmrFI CCNGG 3 cut(s) 504, 794, 1232
BpiI GAAGAC 1 cut(s) 1082
BplI GAGNNNNNCTC 2 cut(s) 959, 991
Bpu10I CCTNAGC 1 cut(s) 580
BpuMI CCSGG 1 cut(s) 504
BsaAI YACGTR 1 cut(s) 778
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 4 cut(s) 350, 444, 467, 1195
BsaJI CCNNGG 2 cut(s) 684, 919
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 1237
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 496
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 2 cut(s) 794, 1232
BseDI CCNNGG 2 cut(s) 684, 919
BseGI GGATG 2 cut(s) 733, 1078
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 2 cut(s) 440, 1237
BseMI GCAATG 1 cut(s) 496
BseMII CTCAG 4 cut(s) 98, 264, 576, 1007
BseNI ACTGG 1 cut(s) 294
BseRI GAGGAG 2 cut(s) 205, 860
BseXI GCAGC 3 cut(s) 21, 376, 1192
BseYI CCCAGC 1 cut(s) 552
BshFI GGCC 1 cut(s) 782
BshNI GGYRCC 2 cut(s) 443, 466
BsiHKAI GWGCWC 1 cut(s) 994
BsiHKCI CYCGRG 1 cut(s) 963
BsiSI CCGG 2 cut(s) 441, 503
BslFI GGGAC 2 cut(s) 607, 937
BslI CCNNNNNNNGG 2 cut(s) 440, 1237
BsmAI GTCTC 3 cut(s) 5, 128, 415
BsmFI GGGAC 2 cut(s) 607, 937
BsnI GGCC 1 cut(s) 782
BsoBI CYCGRG 1 cut(s) 963
Bsp1286I GDGCHC 1 cut(s) 994
Bsp143I GATC 7 cut(s) 284, 373, 461, 571, 789, 1240, 1293
Bsp19I CCATGG 1 cut(s) 684
BspACI CCGC 3 cut(s) 428, 431, 487
BspANI GGCC 1 cut(s) 782
BspCNI CTCAG 4 cut(s) 97, 263, 575, 1008
BspHI TCATGA 1 cut(s) 1296
BspLI GGNNCC 4 cut(s) 445, 468, 623, 798
BspPI GGATC 3 cut(s) 469, 784, 1248
BspT107I GGYRCC 2 cut(s) 443, 466
BspTI CTTAAG 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 496
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 2 cut(s) 684, 919
BssMI GATC 7 cut(s) 284, 373, 461, 571, 789, 1240, 1293
BssNI GRCGYC 4 cut(s) 350, 444, 467, 1195
BssT1I CCWWGG 1 cut(s) 684
Bst2UI CCWGG 2 cut(s) 794, 1232
Bst4CI ACNGT 4 cut(s) 455, 945, 1115, 1175
Bst6I CTCTTC 3 cut(s) 116, 308, 756
BstACI GRCGYC 4 cut(s) 350, 444, 467, 1195
BstAFI CTTAAG 1 cut(s) 134
BstBAI YACGTR 1 cut(s) 778
BstC8I GCNNGC 3 cut(s) 77, 425, 1151
BstDEI CTNAG 6 cut(s) 84, 250, 562, 580, 995, 1016
BstDSI CCRYGG 2 cut(s) 684, 919
BstENI CCTNNNNNAGG 1 cut(s) 1235
BstF5I GGATG 2 cut(s) 733, 1078
BstH2I RGCGCY 2 cut(s) 447, 470
BstHHI GCGC 3 cut(s) 446, 469, 1237
BstKTI GATC 7 cut(s) 287, 376, 464, 574, 792, 1243, 1296
BstMAI GTCTC 3 cut(s) 5, 128, 415
BstMBI GATC 7 cut(s) 284, 373, 461, 571, 789, 1240, 1293
BstMWI GCNNNNNNNGC 2 cut(s) 558, 915
BstNI CCWGG 2 cut(s) 794, 1232
BstNSI RCATGY 2 cut(s) 56, 79
BstSCI CCNGG 3 cut(s) 502, 792, 1230
BstSFI CTRYAG 1 cut(s) 1154
BstV1I GCAGC 3 cut(s) 21, 376, 1192
BstV2I GAAGAC 1 cut(s) 1082
BstXI CCANNNNNNTGG 1 cut(s) 618
BsuRI GGCC 1 cut(s) 782
BtgI CCRYGG 2 cut(s) 684, 919
BtsCI GGATG 2 cut(s) 733, 1078
BtsI GCAGTG 1 cut(s) 1166
BtsIMutI CAGTG 2 cut(s) 744, 1166
Cac8I GCNNGC 3 cut(s) 77, 425, 1151
CaiI CAGNNNCTG 2 cut(s) 569, 865
CciI TCATGA 1 cut(s) 1296
CfoI GCGC 3 cut(s) 446, 469, 1237
Cfr13I GGNCC 5 cut(s) 621, 688, 781, 796, 876
CseI GACGC 1 cut(s) 26
CviAII CATG 7 cut(s) 53, 76, 499, 575, 685, 1045, 1297
DdeI CTNAG 6 cut(s) 84, 250, 562, 580, 995, 1016
DinI GGCGCC 2 cut(s) 445, 468
DpnI GATC 7 cut(s) 286, 375, 463, 573, 791, 1242, 1295
DpnII GATC 7 cut(s) 284, 373, 461, 571, 789, 1240, 1293
Eam1104I CTCTTC 3 cut(s) 116, 308, 756
EarI CTCTTC 3 cut(s) 116, 308, 756
Eco130I CCWWGG 1 cut(s) 684
Eco47I GGWCC 4 cut(s) 621, 688, 796, 876
Eco57I CTGAAG 1 cut(s) 403
Eco72I CACGTG 1 cut(s) 778
Eco88I CYCGRG 1 cut(s) 963
EcoNI CCTNNNNNAGG 1 cut(s) 1235
EcoRII CCWGG 2 cut(s) 792, 1230
EcoT14I CCWWGG 1 cut(s) 684
EgeI GGCGCC 2 cut(s) 445, 468
EheI GGCGCC 2 cut(s) 445, 468
ErhI CCWWGG 1 cut(s) 684
FaeI CATG 7 cut(s) 56, 79, 502, 578, 688, 1048, 1300
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 2 cut(s) 607, 937
FatI CATG 7 cut(s) 52, 75, 498, 574, 684, 1044, 1296
FbaI TGATCA 3 cut(s) 373, 571, 1293
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 6 cut(s) 35, 390, 428, 431, 487, 1206
FokI GGATG 2 cut(s) 740, 1085
Fsp4HI GCNGC 6 cut(s) 35, 390, 428, 431, 487, 1206
FspBI CTAG 2 cut(s) 383, 597
GlaI GCGC 3 cut(s) 445, 468, 1236
GluI GCNGC 6 cut(s) 35, 390, 428, 431, 487, 1206
GsaI CCCAGC 1 cut(s) 556
HaeII RGCGCY 2 cut(s) 447, 470
HaeIII GGCC 1 cut(s) 782
HapII CCGG 2 cut(s) 441, 503
HgaI GACGC 1 cut(s) 26
HhaI GCGC 3 cut(s) 446, 469, 1237
Hin1I GRCGYC 4 cut(s) 350, 444, 467, 1195
Hin1II CATG 7 cut(s) 56, 79, 502, 578, 688, 1048, 1300
Hin6I GCGC 3 cut(s) 444, 467, 1235
HinP1I GCGC 3 cut(s) 444, 467, 1235
HincII GTYRAC 2 cut(s) 1012, 1037
HindII GTYRAC 2 cut(s) 1012, 1037
HindIII AAGCTT 2 cut(s) 137, 824
HinfI GANTC 4 cut(s) 88, 862, 883, 925
HpaII CCGG 2 cut(s) 441, 503
HphI GGTGA 1 cut(s) 1114
Hpy166II GTNNAC 6 cut(s) 602, 744, 772, 876, 1012, 1037
Hpy188III TCNNGA 3 cut(s) 459, 542, 1297
Hpy8I GTNNAC 6 cut(s) 602, 744, 772, 876, 1012, 1037
Hpy99I CGWCG 3 cut(s) 42, 352, 355
HpyAV CCTTC 2 cut(s) 427, 920
HpyCH4III ACNGT 4 cut(s) 455, 945, 1115, 1175
HpyCH4IV ACGT 3 cut(s) 350, 777, 1195
HpyCH4V TGCA 3 cut(s) 75, 79, 1287
HpyF10VI GCNNNNNNNGC 2 cut(s) 558, 915
HpyF3I CTNAG 6 cut(s) 84, 250, 562, 580, 995, 1016
HpySE526I ACGT 3 cut(s) 350, 777, 1195
Hsp92I GRCGYC 4 cut(s) 350, 444, 467, 1195
Hsp92II CATG 7 cut(s) 56, 79, 502, 578, 688, 1048, 1300
HspAI GCGC 3 cut(s) 444, 467, 1235
KasI GGCGCC 2 cut(s) 443, 466
Ksp22I TGATCA 3 cut(s) 373, 571, 1293
Kzo9I GATC 7 cut(s) 284, 373, 461, 571, 789, 1240, 1293
LmnI GCTCC 1 cut(s) 847
Lsp1109I GCAGC 3 cut(s) 21, 376, 1192
MaeI CTAG 2 cut(s) 383, 597
MaeII ACGT 3 cut(s) 350, 777, 1195
MalI GATC 7 cut(s) 286, 375, 463, 573, 791, 1242, 1295
MboI GATC 7 cut(s) 284, 373, 461, 571, 789, 1240, 1293
MhlI GDGCHC 1 cut(s) 994
MluCI AATT 5 cut(s) 6, 70, 172, 1122, 1200
Mly113I GGCGCC 2 cut(s) 444, 467
MlyI GAGTC 2 cut(s) 82, 919
MroXI GAANNNNTTC 1 cut(s) 740
MseI TTAA 4 cut(s) 135, 221, 869, 1226
MslI CAYNNNNRTG 3 cut(s) 57, 749, 918
MspCI CTTAAG 1 cut(s) 134
MspI CCGG 2 cut(s) 441, 503
MspR9I CCNGG 3 cut(s) 504, 794, 1232
MvaI CCWGG 2 cut(s) 794, 1232
MwoI GCNNNNNNNGC 2 cut(s) 558, 915
NarI GGCGCC 2 cut(s) 444, 467
NciI CCSGG 1 cut(s) 504
NcoI CCATGG 1 cut(s) 684
NdeII GATC 7 cut(s) 284, 373, 461, 571, 789, 1240, 1293
NlaIII CATG 7 cut(s) 56, 79, 502, 578, 688, 1048, 1300
NlaIV GGNNCC 4 cut(s) 445, 468, 623, 798
NspI RCATGY 2 cut(s) 56, 79
PaeI GCATGC 1 cut(s) 79
PagI TCATGA 1 cut(s) 1296
PdmI GAANNNNTTC 1 cut(s) 740
PfeI GAWTC 2 cut(s) 862, 883
PfoI TCCNGGA 1 cut(s) 792
PkrI GCNGC 6 cut(s) 36, 391, 429, 432, 488, 1207
PleI GAGTC 2 cut(s) 82, 919
PluTI GGCGCC 2 cut(s) 447, 470
PmaCI CACGTG 1 cut(s) 778
PmlI CACGTG 1 cut(s) 778
PpsI GAGTC 2 cut(s) 82, 919
Ppu21I YACGTR 1 cut(s) 778
PshBI ATTAAT 1 cut(s) 1226
Psp6I CCWGG 2 cut(s) 792, 1230
PspCI CACGTG 1 cut(s) 778
PspFI CCCAGC 1 cut(s) 552
PspGI CCWGG 2 cut(s) 792, 1230
PspN4I GGNNCC 4 cut(s) 445, 468, 623, 798
PspPI GGNCC 5 cut(s) 621, 688, 781, 796, 876
PstNI CAGNNNCTG 2 cut(s) 569, 865
RseI CAYNNNNRTG 3 cut(s) 57, 749, 918
SaqAI TTAA 4 cut(s) 135, 221, 869, 1226
SatI GCNGC 6 cut(s) 35, 390, 428, 431, 487, 1206
Sau3AI GATC 7 cut(s) 284, 373, 461, 571, 789, 1240, 1293
Sau96I GGNCC 5 cut(s) 621, 688, 781, 796, 876
SchI GAGTC 2 cut(s) 82, 919
ScrFI CCNGG 3 cut(s) 504, 794, 1232
SduI GDGCHC 1 cut(s) 994
SfcI CTRYAG 1 cut(s) 1154
SfoI GGCGCC 2 cut(s) 445, 468
SinI GGWCC 4 cut(s) 621, 688, 796, 876
SmiMI CAYNNNNRTG 3 cut(s) 57, 749, 918
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
SphI GCATGC 1 cut(s) 79
Sse9I AATT 5 cut(s) 6, 70, 172, 1122, 1200
SsiI CCGC 3 cut(s) 428, 431, 487
SspDI GGCGCC 2 cut(s) 443, 466
SspI AATATT 1 cut(s) 1052
SspMI CTAG 2 cut(s) 383, 597
StyD4I CCNGG 3 cut(s) 502, 792, 1230
StyI CCWWGG 1 cut(s) 684
TaaI ACNGT 4 cut(s) 455, 945, 1115, 1175
TaiI ACGT 3 cut(s) 353, 780, 1198
TaqI TCGA 1 cut(s) 283
TaqII GACCGA 1 cut(s) 518
TasI AATT 5 cut(s) 6, 70, 172, 1122, 1200
TauI GCSGC 3 cut(s) 430, 433, 489
TfiI GAWTC 2 cut(s) 862, 883
Tru1I TTAA 4 cut(s) 135, 221, 869, 1226
Tru9I TTAA 4 cut(s) 135, 221, 869, 1226
TscAI CASTG 2 cut(s) 751, 1166
TseI GCWGC 3 cut(s) 34, 389, 1205
TspDTI ATGAA 6 cut(s) 507, 881, 902, 1037, 1089, 1277
TspGWI ACGGA 1 cut(s) 876
TspRI CASTG 2 cut(s) 751, 1166
Vha464I CTTAAG 1 cut(s) 134
VpaK11BI GGWCC 4 cut(s) 621, 688, 796, 876
VspI ATTAAT 1 cut(s) 1226
XagI CCTNNNNNAGG 1 cut(s) 1235
XapI RAATTY 1 cut(s) 172
XceI RCATGY 2 cut(s) 56, 79
XmiI GTMKAC 1 cut(s) 771
XmnI GAANNNNTTC 1 cut(s) 740
XspI CTAG 2 cut(s) 383, 597
ZraI GACGTC 2 cut(s) 351, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.