MD08G1089500.v1.1

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009606
Physical Location & Seq
Reverse (-)
7405993 .. 7409547
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1089500.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGGAGGGTTTTCGCGGTTTCCCGGAAACGGCGTCGCTTTGGGCTCCGGAATTTTTGTTGCCGGAATGTGTGCCCGGAAGCTCATCGTCGCAGAACCACATTGGGGGAGAATCCCATTCTGATCAAACCCTGGAGGCTGCCTTTTCTCGCCTAAATGTGTCAGCTTTCAGCCGCCAGCAGCAGCGGATTTCCGGCATTGACTGCGGCAATTACAGTAATGGGCCGGCGCTGAACTACCCATCAAGCGATCACGGATTTAGAGGCGGAAATTCGATGCCTTTTTGCAGCGGCGAGTGGTTGCAGAGTCGTCTGAACTCACAGAACGGCGGGGGTGGGGATTTAGGGAATTTTGGTCTTGAAAATTGCTGGGTGGGAAATGGCTCGAATGGCTTTGTTCCCAATCAAGATTGCTGGGTGCATTCTGACATTGCTGGGTTTCAGGAACCTCTGGATCGCCTGTCGTTGGCTGATTTGCGCGGAAATATCGCTGCTTTGGCCAAGCAGCAGCACGGCTGTCGATTTCTGCAGAAAACAATGGAGGATGCACCGAACAGGGATGTGATTGATTTAATATTCTTGGAGGTTATGGAAAATGTGGTCGTTTTGATGCTCGACCCTTACGGGAACTATGTCGTTCAGAAGCTTGTGGAGGTCTGCACCGAAGAGCAGAGGACCCGTATGCTTTCGAAGTTGATATTGGAGAGTCATACGTCACTGGTTTGTATTGCCCTCGACACTCGCGGAACTCGTAGTGTACAGAAATTGTTGCAGTACGTTACTAACCAAGAGCAAGTGTCGTTGATTATGGGAGCTCTGAGCCCTGCTGCCGCTGTGTTGAGTAAGAACAACAATGGCCAACATGTGATCGAACAGTGCTTAGAGAATTTTTCTGAGGAAGACAACAGGGGTCTTCTGCTTGTGGTGGCTATTAATTGTTCCACAATTGGGAAGGACAAAAGTGGCTGTTGTGTGCTACAAAAGTGCATTGAACACTCTAGTGGTGAAAATAGAGAACGCCTGGTTGCTGCTATCATAGCGCAAGCAACAGTACTTGCAGTAGATCGCTACGGAAACTACGTGGTGCAACATTTACTAGGTTTGAGGATCCCGCAAATCACACAAAATCTTTTGAGACAACTTCAAGGCAGTTATATTTCTATATCATTGAATAAGTTTGGTAGCTGCGTTGTGGAGAAATGCTTGTCCGAGTCAAGCGAAGAACAATCTTCACAGATTATATTTGAGTTGGTCACCAACCCAAATGTGTCAATGCTTCTTGTTCACCAGTATGGGAACTTTGTCATCCAGACAGCACTCGAGGTTTCTAAGGGTATCTACCATCAAGCTCTACTAAATCTGGTTAATTTATACTCCGAATTTCTGCGTGGCAATTCTTATGGAAGAAAAGTACTTGCTCGGCTTGACAGAGCTTCTTCCGCCTACGTTGCAAAGTCACCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

488

Amino Acids

53.5

Weight (kDa)

5.62

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 161 - 195 1.4e-06 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 163 - 468 9.2e-12 NOP9-like PUF repeat domain
PUF PF00806 198 - 232 2.2e-11 Pumilio-family RNA binding repeat
PUF PF00806 311 - 345 1e-11 Pumilio-family RNA binding repeat
PUF PF00806 385 - 416 1.8e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 15, 477, 741
AccIII TCCGGA 1 cut(s) 46
AclWI GGATC 3 cut(s) 459, 1099, 1112
AcoI YGGCCR 2 cut(s) 495, 853
AcsI RAATTY 5 cut(s) 50, 268, 346, 883, 1376
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 4 cut(s) 756, 773, 1050, 1410
AfiI CCNNNNNNNGG 4 cut(s) 28, 103, 463, 945
AflIII ACRYGT 1 cut(s) 859
AgsI TTSAA 4 cut(s) 359, 989, 1142, 1168
AjnI CCWGG 2 cut(s) 129, 1017
AjuI GAANNNNNNNTTGG 2 cut(s) 680, 712
AleI CACNNNNGTG 1 cut(s) 996
AluBI AGCT 7 cut(s) 81, 164, 643, 812, 1182, 1346, 1430
AluI AGCT 7 cut(s) 81, 164, 643, 812, 1182, 1346, 1430
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 1 cut(s) 1126
AlwI GGATC 3 cut(s) 459, 1099, 1112
Ama87I CYCGRG 1 cut(s) 1316
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 3 cut(s) 221, 495, 853
ApoI RAATTY 5 cut(s) 50, 268, 346, 883, 1376
AseI ATTAAT 1 cut(s) 930
AspLEI GCGC 3 cut(s) 229, 477, 1039
AspS9I GGNCC 2 cut(s) 221, 672
AsuC2I CCSGG 2 cut(s) 23, 75
AsuHPI GGTGA 4 cut(s) 1013, 1243, 1274, 1446
AsuII TTCGAA 1 cut(s) 686
AvaI CYCGRG 1 cut(s) 1316
AvaII GGWCC 1 cut(s) 672
BaeGI GKGCMC 1 cut(s) 75
BalI TGGCCA 2 cut(s) 497, 855
BamHI GGATCC 1 cut(s) 1104
BanII GRGCYC 3 cut(s) 46, 814, 821
BbsI GAAGAC 2 cut(s) 902, 903
Bbv12I GWGCWC 1 cut(s) 814
BccI CCATC 2 cut(s) 247, 1347
BceAI ACGGC 3 cut(s) 45, 340, 526
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciT130I CCWGG 2 cut(s) 131, 1019
BclI TGATCA 1 cut(s) 121
BcnI CCSGG 2 cut(s) 23, 75
BcoDI GTCTC 1 cut(s) 1126
BfaI CTAG 2 cut(s) 996, 1094
BfmI CTRYAG 1 cut(s) 524
BfoI RGCGCY 1 cut(s) 230
BmcAI AGTACT 2 cut(s) 1050, 1410
Bme1390I CCNGG 4 cut(s) 23, 75, 131, 1019
Bme18I GGWCC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 1316
BmgT120I GGNCC 2 cut(s) 221, 672
BmiI GGNNCC 4 cut(s) 45, 444, 674, 1106
BmrFI CCNGG 4 cut(s) 23, 75, 131, 1019
BmsI GCATC 3 cut(s) 264, 532, 597
BpiI GAAGAC 2 cut(s) 902, 903
BpmI CTGGAG 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 686
BpuMI CCSGG 2 cut(s) 23, 75
BsaAI YACGTR 1 cut(s) 1078
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 129
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 103, 463, 945
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 2 cut(s) 720, 1285
Bse3DI GCAATG 1 cut(s) 426
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 2 cut(s) 131, 1019
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 3 cut(s) 547, 562, 1302
BseLI CCNNNNNNNGG 4 cut(s) 28, 103, 463, 945
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 2 cut(s) 806, 882
BseNI ACTGG 2 cut(s) 720, 1285
BseSI GKGCMC 1 cut(s) 75
BseYI CCCAGC 3 cut(s) 366, 411, 431
BsgI GTGCAG 1 cut(s) 640
Bsh1236I CGCG 3 cut(s) 15, 477, 741
BshFI GGCC 3 cut(s) 223, 497, 855
BsiHKAI GWGCWC 1 cut(s) 814
BsiHKCI CYCGRG 1 cut(s) 1316
BsiSI CCGG 6 cut(s) 23, 47, 62, 75, 192, 224
BslI CCNNNNNNNGG 4 cut(s) 28, 103, 463, 945
BsmAI GTCTC 1 cut(s) 1126
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 3 cut(s) 223, 497, 855
BsoBI CYCGRG 1 cut(s) 1316
Bsp119I TTCGAA 1 cut(s) 686
Bsp1286I GDGCHC 4 cut(s) 46, 75, 814, 821
Bsp13I TCCGGA 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 754
Bsp143I GATC 6 cut(s) 121, 247, 451, 864, 1060, 1104
BspANI GGCC 3 cut(s) 223, 497, 855
BspCNI CTCAG 2 cut(s) 807, 883
BspEI TCCGGA 1 cut(s) 46
BspFNI CGCG 3 cut(s) 15, 477, 741
BspLI GGNNCC 4 cut(s) 45, 444, 674, 1106
BspMAI CTGCAG 1 cut(s) 528
BspPI GGATC 3 cut(s) 459, 1099, 1112
BspQI GCTCTTC 1 cut(s) 657
BspT104I TTCGAA 1 cut(s) 686
BsrDI GCAATG 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 223
BsrGI TGTACA 1 cut(s) 754
BsrI ACTGG 2 cut(s) 720, 1285
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 6 cut(s) 121, 247, 451, 864, 1060, 1104
BssNI GRCGYC 1 cut(s) 32
Bst2UI CCWGG 2 cut(s) 131, 1019
Bst4CI ACNGT 3 cut(s) 215, 873, 1048
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 754
BstBAI YACGTR 1 cut(s) 1078
BstBI TTCGAA 1 cut(s) 686
BstC8I GCNNGC 3 cut(s) 176, 225, 1041
BstDEI CTNAG 4 cut(s) 815, 877, 891, 1326
BstEII GGTNACC 1 cut(s) 1249
BstF5I GGATG 3 cut(s) 547, 562, 1302
BstFNI CGCG 3 cut(s) 15, 477, 741
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 3 cut(s) 229, 477, 1039
BstKTI GATC 6 cut(s) 124, 250, 454, 867, 1063, 1107
BstMAI GTCTC 1 cut(s) 1126
BstMBI GATC 6 cut(s) 121, 247, 451, 864, 1060, 1104
BstMWI GCNNNNNNNGC 6 cut(s) 201, 387, 494, 1034, 1436, 1445
BstNI CCWGG 2 cut(s) 131, 1019
BstNSI RCATGY 1 cut(s) 863
BstPI GGTNACC 1 cut(s) 1249
BstSCI CCNGG 4 cut(s) 21, 73, 129, 1017
BstSFI CTRYAG 1 cut(s) 524
BstSLI GKGCMC 1 cut(s) 75
BstUI CGCG 3 cut(s) 15, 477, 741
BstV2I GAAGAC 2 cut(s) 902, 903
BstX2I RGATCY 1 cut(s) 1104
BstYI RGATCY 1 cut(s) 1104
BsuRI GGCC 3 cut(s) 223, 497, 855
BtsCI GGATG 3 cut(s) 547, 562, 1302
BtsIMutI CAGTG 2 cut(s) 713, 878
Cac8I GCNNGC 3 cut(s) 176, 225, 1041
CfoI GCGC 3 cut(s) 229, 477, 1039
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 2 cut(s) 221, 672
CseI GACGC 1 cut(s) 21
Csp6I GTAC 4 cut(s) 755, 772, 1049, 1409
CviAII CATG 1 cut(s) 860
CviQI GTAC 4 cut(s) 755, 772, 1049, 1409
DdeI CTNAG 4 cut(s) 815, 877, 891, 1326
DpnI GATC 6 cut(s) 123, 249, 453, 866, 1062, 1106
DpnII GATC 6 cut(s) 121, 247, 451, 864, 1060, 1104
EaeI YGGCCR 2 cut(s) 495, 853
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 2 cut(s) 279, 1426
Ecl136II GAGCTC 1 cut(s) 812
Eco24I GRGCYC 3 cut(s) 46, 814, 821
Eco47I GGWCC 1 cut(s) 672
Eco53kI GAGCTC 1 cut(s) 812
Eco88I CYCGRG 1 cut(s) 1316
Eco91I GGTNACC 1 cut(s) 1249
EcoICRI GAGCTC 1 cut(s) 812
EcoO109I RGGNCCY 1 cut(s) 672
EcoO65I GGTNACC 1 cut(s) 1249
EcoRII CCWGG 2 cut(s) 129, 1017
EcoT38I GRGCYC 3 cut(s) 46, 814, 821
FaeI CATG 1 cut(s) 863
FatI CATG 1 cut(s) 859
FauI CCCGC 2 cut(s) 320, 1116
FbaI TGATCA 1 cut(s) 121
FokI GGATG 3 cut(s) 554, 569, 1289
FriOI GRGCYC 3 cut(s) 46, 814, 821
FspBI CTAG 2 cut(s) 996, 1094
GlaI GCGC 3 cut(s) 228, 476, 1038
GsaI CCCAGC 3 cut(s) 370, 415, 435
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 3 cut(s) 223, 497, 855
HapII CCGG 6 cut(s) 23, 47, 62, 75, 192, 224
HgaI GACGC 1 cut(s) 21
HhaI GCGC 3 cut(s) 229, 477, 1039
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 1 cut(s) 863
Hin6I GCGC 3 cut(s) 227, 475, 1037
HinP1I GCGC 3 cut(s) 227, 475, 1037
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 4 cut(s) 110, 304, 703, 1208
HpaII CCGG 6 cut(s) 23, 47, 62, 75, 192, 224
HphI GGTGA 4 cut(s) 1013, 1243, 1274, 1446
Hpy166II GTNNAC 2 cut(s) 755, 1282
Hpy188I TCNGA 8 cut(s) 121, 312, 424, 639, 816, 892, 1207, 1375
Hpy188III TCNNGA 6 cut(s) 47, 356, 404, 440, 449, 1306
Hpy8I GTNNAC 2 cut(s) 755, 1282
Hpy99I CGWCG 2 cut(s) 37, 91
HpyAV CCTTC 1 cut(s) 943
HpyCH4III ACNGT 3 cut(s) 215, 873, 1048
HpyCH4IV ACGT 4 cut(s) 710, 774, 1077, 1443
HpyF10VI GCNNNNNNNGC 6 cut(s) 201, 387, 494, 1034, 1436, 1445
HpyF3I CTNAG 4 cut(s) 815, 877, 891, 1326
HpySE526I ACGT 4 cut(s) 710, 774, 1077, 1443
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 1 cut(s) 863
HspAI GCGC 3 cut(s) 227, 475, 1037
Kpn2I TCCGGA 1 cut(s) 46
KroI GCCGGC 1 cut(s) 223
KroNI GCCGGC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 6 cut(s) 121, 247, 451, 864, 1060, 1104
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 2 cut(s) 49, 809
LweI GCATC 3 cut(s) 264, 532, 597
MaeI CTAG 2 cut(s) 996, 1094
MaeII ACGT 4 cut(s) 710, 774, 1077, 1443
MaeIII GTNAC 4 cut(s) 711, 775, 1249, 1452
MalI GATC 6 cut(s) 123, 249, 453, 866, 1062, 1106
MboI GATC 6 cut(s) 121, 247, 451, 864, 1060, 1104
MboII GAAGA 7 cut(s) 674, 902, 908, 1218, 1229, 1413, 1425
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 1 cut(s) 1104
MhlI GDGCHC 4 cut(s) 46, 75, 814, 821
MlsI TGGCCA 2 cut(s) 497, 855
MluNI TGGCCA 2 cut(s) 497, 855
MlyI GAGTC 3 cut(s) 313, 712, 1217
Mox20I TGGCCA 2 cut(s) 497, 855
MroI TCCGGA 1 cut(s) 46
MroNI GCCGGC 1 cut(s) 223
MscI TGGCCA 2 cut(s) 497, 855
MseI TTAA 3 cut(s) 569, 930, 1362
MslI CAYNNNNRTG 2 cut(s) 996, 1287
Msp20I TGGCCA 2 cut(s) 497, 855
MspA1I CMGCKG 3 cut(s) 184, 288, 830
MspI CCGG 6 cut(s) 23, 47, 62, 75, 192, 224
MspR9I CCNGG 4 cut(s) 23, 75, 131, 1019
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 2 cut(s) 131, 1019
MvnI CGCG 3 cut(s) 15, 477, 741
MwoI GCNNNNNNNGC 6 cut(s) 201, 387, 494, 1034, 1436, 1445
NaeI GCCGGC 1 cut(s) 225
NciI CCSGG 2 cut(s) 23, 75
NdeII GATC 6 cut(s) 121, 247, 451, 864, 1060, 1104
NgoMIV GCCGGC 1 cut(s) 223
NlaIII CATG 1 cut(s) 863
NlaIV GGNNCC 4 cut(s) 45, 444, 674, 1106
NmeAIII GCCGAG 1 cut(s) 1396
NmuCI GTSAC 3 cut(s) 711, 1249, 1452
NspI RCATGY 1 cut(s) 863
NspV TTCGAA 1 cut(s) 686
OliI CACNNNNGTG 1 cut(s) 996
PaeR7I CTCGAG 1 cut(s) 1316
PciI ACATGT 1 cut(s) 859
PciSI GCTCTTC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 2 cut(s) 745, 1074
PctI GAATGC 1 cut(s) 418
PdiI GCCGGC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 110
PfoI TCCNGGA 1 cut(s) 21
PleI GAGTC 3 cut(s) 312, 711, 1216
PpsI GAGTC 3 cut(s) 312, 711, 1216
Ppu21I YACGTR 1 cut(s) 1078
PpuMI RGGWCCY 1 cut(s) 672
PscI ACATGT 1 cut(s) 859
PshBI ATTAAT 1 cut(s) 930
Psp124BI GAGCTC 1 cut(s) 814
Psp5II RGGWCCY 1 cut(s) 672
Psp6I CCWGG 2 cut(s) 129, 1017
PspEI GGTNACC 1 cut(s) 1249
PspFI CCCAGC 3 cut(s) 366, 411, 431
PspGI CCWGG 2 cut(s) 129, 1017
PspN4I GGNNCC 4 cut(s) 45, 444, 674, 1106
PspPI GGNCC 2 cut(s) 221, 672
PspPPI RGGWCCY 1 cut(s) 672
PspXI VCTCGAGB 1 cut(s) 1316
PstI CTGCAG 1 cut(s) 528
PsuI RGATCY 1 cut(s) 1104
RsaI GTAC 4 cut(s) 756, 773, 1050, 1410
RsaNI GTAC 4 cut(s) 755, 772, 1049, 1409
RseI CAYNNNNRTG 2 cut(s) 996, 1287
SacI GAGCTC 1 cut(s) 814
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 3 cut(s) 569, 930, 1362
Sau3AI GATC 6 cut(s) 121, 247, 451, 864, 1060, 1104
Sau96I GGNCC 2 cut(s) 221, 672
ScaI AGTACT 2 cut(s) 1050, 1410
SchI GAGTC 3 cut(s) 313, 712, 1217
ScrFI CCNGG 4 cut(s) 23, 75, 131, 1019
SduI GDGCHC 4 cut(s) 46, 75, 814, 821
SfaNI GCATC 3 cut(s) 264, 532, 597
SfcI CTRYAG 1 cut(s) 524
Sfr274I CTCGAG 1 cut(s) 1316
SfuI TTCGAA 1 cut(s) 686
SinI GGWCC 1 cut(s) 672
SlaI CTCGAG 1 cut(s) 1316
SmiMI CAYNNNNRTG 2 cut(s) 996, 1287
SmlI CTYRAG 1 cut(s) 1316
SmoI CTYRAG 1 cut(s) 1316
SspI AATATT 1 cut(s) 573
SspMI CTAG 2 cut(s) 996, 1094
SstI GAGCTC 1 cut(s) 814
StyD4I CCNGG 4 cut(s) 21, 73, 129, 1017
TaaI ACNGT 3 cut(s) 215, 873, 1048
TaiI ACGT 4 cut(s) 713, 777, 1080, 1446
TaqI TCGA 8 cut(s) 272, 383, 517, 612, 686, 732, 867, 1317
TatI WGTACW 3 cut(s) 754, 1048, 1408
TauI GCSGC 4 cut(s) 174, 207, 291, 830
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 3 cut(s) 569, 930, 1362
Tru9I TTAA 3 cut(s) 569, 930, 1362
TscAI CASTG 2 cut(s) 720, 878
TseFI GTSAC 3 cut(s) 711, 1249, 1452
Tsp45I GTSAC 3 cut(s) 711, 1249, 1452
TspGWI ACGGA 2 cut(s) 267, 1083
TspRI CASTG 2 cut(s) 720, 878
VpaK11BI GGWCC 1 cut(s) 672
VspI ATTAAT 1 cut(s) 930
XapI RAATTY 5 cut(s) 50, 268, 346, 883, 1376
XceI RCATGY 1 cut(s) 863
XhoI CTCGAG 1 cut(s) 1316
XspI CTAG 2 cut(s) 996, 1094
ZrmI AGTACT 2 cut(s) 1050, 1410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.