MD15G1074100.v1.1

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009606
Physical Location & Seq
Reverse (-)
5060887 .. 5064539
3653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1074100.v1.1.491

Sequence Viewer

Length: 1779 bp
ATGCAAACTCACACCCCTATTGAAACTCTCTCACTTCCCATTTCAGTCTCTGACTGGTCAAGCTTTTCCCCTTGTTTTTCAATTGCCCCCCAATTAAGAATCTTCCTCTCAGTCTCTCCCCCATCGCAATTTCAGAATCCCCCTATCTCTCAAAAAACCTCCGAAAGTTCGAAGCTTTTTCTCTCTCAAGACTCCAGAGTCGGAATCTCATCCCTGTTGAGGCGGAGAATGGAGGGTTTTCGGTGCTTCCCGGAAACGAAGTCGCTTTGGGCTCCGAAATTTTCGTCGCCAGAATGTGTGCCCGGAAGCTCATCGTCGCAGAACCACATTGGGGGAGAATCCCATTCTGATCAAACCCTGGAGGCTGCCTTTTCTCGCCTAAATGTGTCAGCTTTCAGCCGCCAGCAGCCGCGTATTTCTGGCATTGACGGCGGAGATCACAGTAATGGATCGGCGCTGAACTACCCATCAAGCGAACACGGATATAGCGGCGGAAATTCGTTGCCTTTTGGAGGCGGAGAGTCGTTACAGAGTGCTCTGAGCTTGCAGAGCGGCGGGGATCGGGTTTTTGGGAACCTTGGTCTTGAAAATTGCTGGGTGGACTCTGATATTACTAATGGCTCGAATGGCTTTGTTCCCAATCAAGAGTGCTGGGTGCATTCTGACATTGCTGGGTTTAGTGAAACACTGGATCGGTTGTCGTTGGCTGATTTGCGCGGAAAAATTGCTGCTTTGGCCAAGGAGCAGCACGGCTGTCGTTTTCTGCAGAAAACAATGGATGTTGCACCGAACAGGGATGTGATTGATTTGATATTCTTGGAGGTTATGGAAAATGTGGTCGTTTTGATGCTTGACCCTCACGGGAACTATGTCGTCCAGAAGCTTGTGGAGGTCTGCACTGAAGAGCAGAGGACCCGTATGCTTTCGAAGTTGATAATGGAGAGTCATACGTCACTGGTTTGTATTGCCCTGGACAATCGCGGGACTCGCACTGTACAGAAATTATTGCGGTATGTTACTAACCAAGAGCAAGTGTCGCTGATTATGGGAGCTCTGAGCCCTGGTGCCGCTGTGTTGAGTAAGAACAACAACGGCCAACATGTGATCGAATATTGCTTAGAGAATTTTTCTGAGGAAGACAACAGGGGCCTTCTATGTGTGGTGGCTATTAACTGTTCTACGATTGGGAAGGACAAAAGTGGCTGTTGTGTGCTACAAAAGTGCGTTGAGCACTCTAGTGGTGAAAATAGAGAACGCCTGGTTAATGCTGTCATAGGGAAAGCAACAGTACTTGCAGTAGATCGCTACGGAAACTACGTGGTGCAACATTTACTGGGTTTGAGGATCCCGCGAATCACACAAAATCTTTTGAGACAACTTCATGGCAGTTATATTTCTGTATCGTTGAATAAGTACGGTAGTTGCGTTGTGGAGAAATGCTTGTCTGAGTCAAGCGAAGAACAATCTTCACAGATTATATTTGAGCTGGTTACCCACCCAAATGTGTCAATGCTTCTTGTTCACCAGTATGGGAACTATGTCATCCAGACAGCACTCGAGGTTTCTAAGGGTATCTACCATGAAGCTCTACTAAATCTGGTTGGTTTATACTCCGACTTCTTGCGTGGGAATTCTTATGGAAGAAAGGTACTGGCTCGGCGTGACAGGGTTTCTTCCGCCTACATTTCAAGGTCACATTTGTCGGTAGGGCTGCAAGGGTTCGTTAATTGGAACAGTGGAAAATATGCGTGTGGATATCAGCAGCCGGGTAGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

593

Amino Acids

65.05

Weight (kDa)

6.78

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF_NOP9 PF22493 243 - 548 1.7e-12 NOP9-like PUF repeat domain
PUF PF00806 278 - 312 2.7e-11 Pumilio-family RNA binding repeat
PUF PF00806 391 - 425 5.2e-11 Pumilio-family RNA binding repeat
PUF PF00806 465 - 496 2e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 1064
AccBSI CCGCTC 1 cut(s) 552
AccII CGCG 4 cut(s) 412, 717, 981, 1351
AclWI GGATC 5 cut(s) 457, 567, 699, 1339, 1352
AcoI YGGCCR 2 cut(s) 735, 1093
AcsI RAATTY 4 cut(s) 278, 496, 1123, 1630
AcuI CTGAAG 1 cut(s) 921
AfaI GTAC 4 cut(s) 996, 1290, 1415, 1650
AfiI CCNNNNNNNGG 3 cut(s) 219, 331, 512
AflIII ACRYGT 1 cut(s) 1099
AgsI TTSAA 5 cut(s) 23, 81, 587, 1408, 1689
AjnI CCWGG 4 cut(s) 357, 969, 1060, 1257
AleI CACNNNNGTG 1 cut(s) 1236
Alw21I GWGCWC 3 cut(s) 538, 1054, 1233
Alw26I GTCTC 3 cut(s) 52, 118, 1366
AlwI GGATC 5 cut(s) 457, 567, 699, 1339, 1352
AlwNI CAGNNNCTG 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 1556
AoxI GGCC 3 cut(s) 735, 1093, 1147
ApeKI GCWGC 6 cut(s) 365, 406, 728, 745, 1711, 1762
ApoI RAATTY 4 cut(s) 278, 496, 1123, 1630
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AspLEI GCGC 2 cut(s) 457, 717
AspS9I GGNCC 2 cut(s) 912, 1147
AsuC2I CCSGG 3 cut(s) 251, 303, 1767
AsuHPI GGTGA 2 cut(s) 1253, 1514
AsuII TTCGAA 2 cut(s) 170, 926
AvaI CYCGRG 1 cut(s) 1556
AvaII GGWCC 1 cut(s) 912
BaeGI GKGCMC 1 cut(s) 303
BalI TGGCCA 1 cut(s) 737
BamHI GGATCC 1 cut(s) 1344
BanI GGYRCC 1 cut(s) 1064
BanII GRGCYC 3 cut(s) 274, 1054, 1061
BbsI GAAGAC 1 cut(s) 1143
Bbv12I GWGCWC 3 cut(s) 538, 1054, 1233
BbvI GCAGC 6 cut(s) 352, 418, 715, 757, 1698, 1774
BccI CCATC 2 cut(s) 130, 475
BceAI ACGGC 3 cut(s) 445, 766, 1108
BcgI CGANNNNNNTGC 2 cut(s) 737, 771
BciT130I CCWGG 4 cut(s) 359, 971, 1062, 1259
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 3 cut(s) 251, 303, 1767
BcoDI GTCTC 3 cut(s) 52, 118, 1366
BfaI CTAG 1 cut(s) 1236
BfmI CTRYAG 1 cut(s) 764
BfoI RGCGCY 1 cut(s) 458
BmcAI AGTACT 1 cut(s) 1290
Bme1390I CCNGG 7 cut(s) 251, 303, 359, 971, 1062, 1259, 1767
Bme18I GGWCC 1 cut(s) 912
BmeT110I CYCGRG 1 cut(s) 1556
BmgT120I GGNCC 2 cut(s) 912, 1147
BmiI GGNNCC 6 cut(s) 273, 575, 914, 1066, 1148, 1346
BmrFI CCNGG 7 cut(s) 251, 303, 359, 971, 1062, 1259, 1767
BmrI ACTGGG 1 cut(s) 1343
BmsI GCATC 1 cut(s) 837
BmuI ACTGGG 1 cut(s) 1343
BpiI GAAGAC 1 cut(s) 1143
BpmI CTGGAG 2 cut(s) 178, 380
Bpu14I TTCGAA 2 cut(s) 170, 926
BpuEI CTTGAG 1 cut(s) 171
BpuMI CCSGG 3 cut(s) 251, 303, 1767
BsaAI YACGTR 1 cut(s) 1318
BsaJI CCNNGG 5 cut(s) 357, 577, 738, 969, 1060
Bsc4I CCNNNNNNNGG 3 cut(s) 219, 331, 512
Bse1I ACTGG 6 cut(s) 59, 693, 960, 1338, 1525, 1656
Bse3DI GCAATG 1 cut(s) 666
BseBI CCWGG 4 cut(s) 359, 971, 1062, 1259
BseDI CCNNGG 5 cut(s) 357, 577, 738, 969, 1060
BseGI GGATG 4 cut(s) 209, 784, 802, 1542
BseLI CCNNNNNNNGG 3 cut(s) 219, 331, 512
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 5 cut(s) 123, 530, 1046, 1122, 1437
BseNI ACTGG 6 cut(s) 59, 693, 960, 1338, 1525, 1656
BseSI GKGCMC 1 cut(s) 303
BseXI GCAGC 6 cut(s) 352, 418, 715, 757, 1698, 1774
BseYI CCCAGC 3 cut(s) 594, 651, 671
BsgI GTGCAG 1 cut(s) 880
Bsh1236I CGCG 4 cut(s) 412, 717, 981, 1351
BshFI GGCC 3 cut(s) 737, 1095, 1149
BshNI GGYRCC 1 cut(s) 1064
BsiHKAI GWGCWC 3 cut(s) 538, 1054, 1233
BsiHKCI CYCGRG 1 cut(s) 1556
BsiSI CCGG 3 cut(s) 251, 303, 1766
BslFI GGGAC 1 cut(s) 997
BslI CCNNNNNNNGG 3 cut(s) 219, 331, 512
BsmAI GTCTC 3 cut(s) 52, 118, 1366
BsmFI GGGAC 1 cut(s) 997
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 3 cut(s) 737, 1095, 1149
BsoBI CYCGRG 1 cut(s) 1556
Bsp119I TTCGAA 2 cut(s) 170, 926
Bsp1286I GDGCHC 6 cut(s) 274, 303, 538, 1054, 1061, 1233
Bsp1407I TGTACA 1 cut(s) 994
Bsp143I GATC 8 cut(s) 349, 436, 449, 559, 691, 1104, 1300, 1344
BspANI GGCC 3 cut(s) 737, 1095, 1149
BspCNI CTCAG 5 cut(s) 122, 531, 1047, 1123, 1438
BspFNI CGCG 4 cut(s) 412, 717, 981, 1351
BspLI GGNNCC 6 cut(s) 273, 575, 914, 1066, 1148, 1346
BspMAI CTGCAG 1 cut(s) 768
BspPI GGATC 5 cut(s) 457, 567, 699, 1339, 1352
BspQI GCTCTTC 1 cut(s) 897
BspT104I TTCGAA 2 cut(s) 170, 926
BspT107I GGYRCC 1 cut(s) 1064
BsrBI CCGCTC 1 cut(s) 552
BsrDI GCAATG 1 cut(s) 666
BsrGI TGTACA 1 cut(s) 994
BsrI ACTGG 6 cut(s) 59, 693, 960, 1338, 1525, 1656
BssECI CCNNGG 5 cut(s) 357, 577, 738, 969, 1060
BssMI GATC 8 cut(s) 349, 436, 449, 559, 691, 1104, 1300, 1344
BssT1I CCWWGG 2 cut(s) 577, 738
Bst2UI CCWGG 4 cut(s) 359, 971, 1062, 1259
Bst4CI ACNGT 6 cut(s) 443, 994, 1175, 1288, 1418, 1736
Bst6I CTCTTC 1 cut(s) 897
BstAUI TGTACA 1 cut(s) 994
BstBAI YACGTR 1 cut(s) 1318
BstBI TTCGAA 2 cut(s) 170, 926
BstC8I GCNNGC 2 cut(s) 404, 545
BstDEI CTNAG 7 cut(s) 109, 539, 1055, 1117, 1131, 1446, 1566
BstEII GGTNACC 1 cut(s) 1489
BstENI CCTNNNNNAGG 1 cut(s) 510
BstF5I GGATG 4 cut(s) 209, 784, 802, 1542
BstFNI CGCG 4 cut(s) 412, 717, 981, 1351
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 2 cut(s) 457, 717
BstKTI GATC 8 cut(s) 352, 439, 452, 562, 694, 1107, 1303, 1347
BstMAI GTCTC 3 cut(s) 52, 118, 1366
BstMBI GATC 8 cut(s) 349, 436, 449, 559, 691, 1104, 1300, 1344
BstMWI GCNNNNNNNGC 6 cut(s) 429, 549, 627, 734, 987, 1036
BstNI CCWGG 4 cut(s) 359, 971, 1062, 1259
BstNSI RCATGY 1 cut(s) 1103
BstPI GGTNACC 1 cut(s) 1489
BstSCI CCNGG 7 cut(s) 249, 301, 357, 969, 1060, 1257, 1765
BstSFI CTRYAG 1 cut(s) 764
BstSLI GKGCMC 1 cut(s) 303
BstUI CGCG 4 cut(s) 412, 717, 981, 1351
BstV1I GCAGC 6 cut(s) 352, 418, 715, 757, 1698, 1774
BstV2I GAAGAC 1 cut(s) 1143
BstX2I RGATCY 1 cut(s) 1344
BstYI RGATCY 1 cut(s) 1344
BsuRI GGCC 3 cut(s) 737, 1095, 1149
BtgZI GCGATG 1 cut(s) 108
BtsCI GGATG 4 cut(s) 209, 784, 802, 1542
BtsIMutI CAGTG 5 cut(s) 686, 897, 953, 990, 1741
Cac8I GCNNGC 2 cut(s) 404, 545
CaiI CAGNNNCTG 1 cut(s) 50
CfoI GCGC 2 cut(s) 457, 717
Cfr13I GGNCC 2 cut(s) 912, 1147
Csp6I GTAC 4 cut(s) 995, 1289, 1414, 1649
CviAII CATG 3 cut(s) 1100, 1382, 1580
CviQI GTAC 4 cut(s) 995, 1289, 1414, 1649
DdeI CTNAG 7 cut(s) 109, 539, 1055, 1117, 1131, 1446, 1566
DpnI GATC 8 cut(s) 351, 438, 451, 561, 693, 1106, 1302, 1346
DpnII GATC 8 cut(s) 349, 436, 449, 559, 691, 1104, 1300, 1344
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 2 cut(s) 735, 1093
Eam1104I CTCTTC 1 cut(s) 897
EarI CTCTTC 1 cut(s) 897
EciI GGCGGA 5 cut(s) 238, 447, 507, 531, 1666
Ecl136II GAGCTC 1 cut(s) 1052
Eco130I CCWWGG 2 cut(s) 577, 738
Eco24I GRGCYC 3 cut(s) 274, 1054, 1061
Eco32I GATATC 1 cut(s) 1757
Eco47I GGWCC 1 cut(s) 912
Eco53kI GAGCTC 1 cut(s) 1052
Eco57I CTGAAG 1 cut(s) 921
Eco88I CYCGRG 1 cut(s) 1556
Eco91I GGTNACC 1 cut(s) 1489
EcoICRI GAGCTC 1 cut(s) 1052
EcoNI CCTNNNNNAGG 1 cut(s) 510
EcoO109I RGGNCCY 2 cut(s) 912, 1147
EcoO65I GGTNACC 1 cut(s) 1489
EcoRI GAATTC 1 cut(s) 1630
EcoRII CCWGG 4 cut(s) 357, 969, 1060, 1257
EcoRV GATATC 1 cut(s) 1757
EcoT14I CCWWGG 2 cut(s) 577, 738
EcoT38I GRGCYC 3 cut(s) 274, 1054, 1061
ErhI CCWWGG 2 cut(s) 577, 738
FaeI CATG 3 cut(s) 1103, 1385, 1583
FaqI GGGAC 1 cut(s) 997
FatI CATG 3 cut(s) 1099, 1381, 1579
FauI CCCGC 3 cut(s) 548, 974, 1356
FbaI TGATCA 1 cut(s) 349
FokI GGATG 4 cut(s) 196, 791, 809, 1529
FriOI GRGCYC 3 cut(s) 274, 1054, 1061
FspBI CTAG 1 cut(s) 1236
GlaI GCGC 2 cut(s) 456, 716
GsaI CCCAGC 3 cut(s) 598, 655, 675
GsuI CTGGAG 2 cut(s) 178, 380
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 3 cut(s) 737, 1095, 1149
HapII CCGG 3 cut(s) 251, 303, 1766
HhaI GCGC 2 cut(s) 457, 717
Hin1II CATG 3 cut(s) 1103, 1385, 1583
Hin6I GCGC 2 cut(s) 455, 715
HinP1I GCGC 2 cut(s) 455, 715
HindIII AAGCTT 3 cut(s) 61, 173, 881
HpaII CCGG 3 cut(s) 251, 303, 1766
HphI GGTGA 2 cut(s) 1253, 1514
Hpy166II GTNNAC 2 cut(s) 601, 1522
Hpy188III TCNNGA 6 cut(s) 188, 195, 584, 644, 877, 1546
Hpy8I GTNNAC 2 cut(s) 601, 1522
Hpy99I CGWCG 2 cut(s) 289, 319
HpyAV CCTTC 2 cut(s) 1160, 1183
HpyCH4III ACNGT 6 cut(s) 443, 994, 1175, 1288, 1418, 1736
HpyCH4IV ACGT 2 cut(s) 950, 1317
HpyCH4V TGCA 9 cut(s) 4, 547, 658, 766, 785, 897, 1295, 1324, 1714
HpyF10VI GCNNNNNNNGC 6 cut(s) 429, 549, 627, 734, 987, 1036
HpyF3I CTNAG 7 cut(s) 109, 539, 1055, 1117, 1131, 1446, 1566
HpySE526I ACGT 2 cut(s) 950, 1317
Hsp92II CATG 3 cut(s) 1103, 1385, 1583
HspAI GCGC 2 cut(s) 455, 715
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 8 cut(s) 349, 436, 449, 559, 691, 1104, 1300, 1344
LguI GCTCTTC 1 cut(s) 897
LmnI GCTCC 4 cut(s) 277, 742, 1049, 1773
Lsp1109I GCAGC 6 cut(s) 352, 418, 715, 757, 1698, 1774
LweI GCATC 1 cut(s) 837
MaeI CTAG 1 cut(s) 1236
MaeII ACGT 2 cut(s) 950, 1317
MaeIII GTNAC 6 cut(s) 525, 951, 1015, 1489, 1661, 1692
MalI GATC 8 cut(s) 351, 438, 451, 561, 693, 1106, 1302, 1346
MbiI CCGCTC 1 cut(s) 552
MboI GATC 8 cut(s) 349, 436, 449, 559, 691, 1104, 1300, 1344
MboII GAAGA 7 cut(s) 94, 914, 1148, 1458, 1469, 1653, 1665
MfeI CAATTG 1 cut(s) 81
MflI RGATCY 1 cut(s) 1344
MhlI GDGCHC 6 cut(s) 274, 303, 538, 1054, 1061, 1233
MlsI TGGCCA 1 cut(s) 737
MluNI TGGCCA 1 cut(s) 737
MlyI GAGTC 7 cut(s) 185, 207, 530, 596, 952, 979, 1457
MmeI TCCRAC 2 cut(s) 181, 1638
Mox20I TGGCCA 1 cut(s) 737
MscI TGGCCA 1 cut(s) 737
MseI TTAA 4 cut(s) 95, 1170, 1263, 1725
MslI CAYNNNNRTG 4 cut(s) 444, 1236, 1500, 1527
Msp20I TGGCCA 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 1070
MspI CCGG 3 cut(s) 251, 303, 1766
MspR9I CCNGG 7 cut(s) 251, 303, 359, 971, 1062, 1259, 1767
MunI CAATTG 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 4 cut(s) 359, 971, 1062, 1259
MvnI CGCG 4 cut(s) 412, 717, 981, 1351
MwoI GCNNNNNNNGC 6 cut(s) 429, 549, 627, 734, 987, 1036
NciI CCSGG 3 cut(s) 251, 303, 1767
NdeII GATC 8 cut(s) 349, 436, 449, 559, 691, 1104, 1300, 1344
NlaIII CATG 3 cut(s) 1103, 1385, 1583
NlaIV GGNNCC 6 cut(s) 273, 575, 914, 1066, 1148, 1346
NmeAIII GCCGAG 1 cut(s) 1636
NmuCI GTSAC 3 cut(s) 951, 1661, 1692
NspI RCATGY 1 cut(s) 1103
NspV TTCGAA 2 cut(s) 170, 926
OliI CACNNNNGTG 1 cut(s) 1236
PaeR7I CTCGAG 1 cut(s) 1556
PciI ACATGT 1 cut(s) 1099
PciSI GCTCTTC 1 cut(s) 897
PcsI WCGNNNNNNNCGW 2 cut(s) 1314, 1422
PctI GAATGC 1 cut(s) 658
PfeI GAWTC 5 cut(s) 99, 136, 204, 338, 1353
PfoI TCCNGGA 1 cut(s) 249
PleI GAGTC 7 cut(s) 185, 206, 529, 596, 951, 979, 1456
PpsI GAGTC 7 cut(s) 185, 206, 529, 596, 951, 979, 1456
Ppu21I YACGTR 1 cut(s) 1318
PpuMI RGGWCCY 1 cut(s) 912
PscI ACATGT 1 cut(s) 1099
Psp124BI GAGCTC 1 cut(s) 1054
Psp5II RGGWCCY 1 cut(s) 912
Psp6I CCWGG 4 cut(s) 357, 969, 1060, 1257
PspEI GGTNACC 1 cut(s) 1489
PspFI CCCAGC 3 cut(s) 594, 651, 671
PspGI CCWGG 4 cut(s) 357, 969, 1060, 1257
PspN4I GGNNCC 6 cut(s) 273, 575, 914, 1066, 1148, 1346
PspPI GGNCC 2 cut(s) 912, 1147
PspPPI RGGWCCY 1 cut(s) 912
PspXI VCTCGAGB 1 cut(s) 1556
PstI CTGCAG 1 cut(s) 768
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 1344
RsaI GTAC 4 cut(s) 996, 1290, 1415, 1650
RsaNI GTAC 4 cut(s) 995, 1289, 1414, 1649
RseI CAYNNNNRTG 4 cut(s) 444, 1236, 1500, 1527
SacI GAGCTC 1 cut(s) 1054
SapI GCTCTTC 1 cut(s) 897
SaqAI TTAA 4 cut(s) 95, 1170, 1263, 1725
Sau3AI GATC 8 cut(s) 349, 436, 449, 559, 691, 1104, 1300, 1344
Sau96I GGNCC 2 cut(s) 912, 1147
ScaI AGTACT 1 cut(s) 1290
SchI GAGTC 7 cut(s) 185, 207, 530, 596, 952, 979, 1457
ScrFI CCNGG 7 cut(s) 251, 303, 359, 971, 1062, 1259, 1767
SduI GDGCHC 6 cut(s) 274, 303, 538, 1054, 1061, 1233
SfaNI GCATC 1 cut(s) 837
SfcI CTRYAG 1 cut(s) 764
Sfr274I CTCGAG 1 cut(s) 1556
SfuI TTCGAA 2 cut(s) 170, 926
SinI GGWCC 1 cut(s) 912
SlaI CTCGAG 1 cut(s) 1556
SmiMI CAYNNNNRTG 4 cut(s) 444, 1236, 1500, 1527
SmlI CTYRAG 2 cut(s) 186, 1556
SmoI CTYRAG 2 cut(s) 186, 1556
SspI AATATT 1 cut(s) 1112
SspMI CTAG 1 cut(s) 1236
SstI GAGCTC 1 cut(s) 1054
StyD4I CCNGG 7 cut(s) 249, 301, 357, 969, 1060, 1257, 1765
StyI CCWWGG 2 cut(s) 577, 738
TaaI ACNGT 6 cut(s) 443, 994, 1175, 1288, 1418, 1736
TaiI ACGT 2 cut(s) 953, 1320
TaqI TCGA 5 cut(s) 170, 623, 926, 1107, 1557
TatI WGTACW 2 cut(s) 994, 1288
TauI GCSGC 5 cut(s) 402, 412, 492, 555, 1070
TfiI GAWTC 5 cut(s) 99, 136, 204, 338, 1353
Tru1I TTAA 4 cut(s) 95, 1170, 1263, 1725
Tru9I TTAA 4 cut(s) 95, 1170, 1263, 1725
TscAI CASTG 5 cut(s) 693, 904, 960, 997, 1741
TseFI GTSAC 3 cut(s) 951, 1661, 1692
TseI GCWGC 6 cut(s) 365, 406, 728, 745, 1711, 1762
Tsp45I GTSAC 3 cut(s) 951, 1661, 1692
TspDTI ATGAA 2 cut(s) 1370, 1596
TspGWI ACGGA 2 cut(s) 495, 1323
TspRI CASTG 5 cut(s) 693, 904, 960, 997, 1741
VpaK11BI GGWCC 1 cut(s) 912
XagI CCTNNNNNAGG 1 cut(s) 510
XapI RAATTY 4 cut(s) 278, 496, 1123, 1630
XceI RCATGY 1 cut(s) 1103
XhoI CTCGAG 1 cut(s) 1556
XspI CTAG 1 cut(s) 1236
ZrmI AGTACT 1 cut(s) 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.