Rh6AG482400

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009606
Physical Location & Seq
Forward (+)
66263807 .. 66267430
3624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG482400.1

Sequence Viewer

Length: 1410 bp
ATGGAGGGTTTCCGATACTTTACCGATACGACGTCGCAGAACCCTATTCCCCATGATCAATCCCTAGAAGCTGCTTTCTCTCGCCTGAATGTCTCATCCTTCAACCAGCCGCCGCCGTTTTCCGGCGCCGATTACAGTTCTGGGTCGGCGCCGAAATACCCATCACGCCGCAATGAACTCATGCCGGGTGGTTTTGATGGTGGGGATTTCGGTCATTTGGGTCTTGAAGATTGCTGGGTTGGCTCAGATACTGATCATGGCTTAGGTGGGTTTGGTTCTAGTTTACATAAACCATCTGATTGGTCCCAAGAACCACTCAACAGCTTATCTGTGGCTGATTTGCGTGGAAAGATTGCTTCTTTGTCCATGGACCAATACGGGTGTCGGTTCTTTCAGAGAGCTATGGAGGATGAGCCGAAAGAGTTCACTGATATGATATTTGAAGAGGTTGTAGACCATGTGGCCCATTTGATTCTGGACCCTTATGGCAACTATGTTGTTCAGAAGCTTGTGGAGGTTTGTAGTGAGGAGCAGAGGACACAGATTCTGTTAAGGTGGACCAAGAATCCGTTCCATTTTATTACTCTTTGCCTTCATAGCCGTGGGACTCGCTCTGTTCTGAAACTGTTGAAGAAAATCACTACCCGAGAGCAAATCTCTGTCTTTGTGAGTGCTCTAAGCTGTGGTGCTGTTGACCTGAGTATGAACATCAATGGTCAACATATCATGGAATATTGCTTGAAGAACTTTTCGGATGAACACAAAAGGTGTCTTCTGAATGTGGTGATCGACAACTGTATTGGAATTGCAAAAGATAAGGTTGGTTGTTGCGTGCTACAGCAGTGTGTTGATTACTGTTATGGAGAAACAAAAGGACGTCTAATTGCTGCGATAATAGAAGATGCATTAATCCTGGCGCAGGATCGTTATGGGTTTGTTCCTATGAAACTATATCAATACTTCTCTTGCAGTTTGATCATGACCTTTACTCTGATGATTTTAATTCAATGTGATATTTTCTTTTCATTCCTTAGCAACTATGTGCTGCAACATTTACTCGGGTTGAGGGCACCACAAGTCACATCAAATCTTCTGAGACAGTTTCAAGGGAATTATATGTTTCTCTCCTGCAGCAAGTATGGTAGTAATGTTGTGGAGAAGTGCTTGTTAGAGTCGGGAGAAGAGCAGTCTGCAGAAATTATTAGAGAGTTGTGTAGAAGCCCAAATCTTTCAATGCTTCTTATGCATCCTTTCGGAAATTATGTCTTCCAGACGGCATTGTCAGTTTCCAAGGGTCTCACCCATAAGTTTCTGCTGAGTCTGGTTCAACATTACACCCCAGCTCTGCTCAGCAATCAATATGGAAAAAGGGTGCTTGCTTGGCGTGAAAGGAACTTGCGACGTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

469

Amino Acids

53.12

Weight (kDa)

6.4

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 118 - 150 5.7e-08 Pumilio-family RNA binding repeat
PUF PF00806 153 - 183 1.8e-10 Pumilio-family RNA binding repeat
PUF PF00806 265 - 299 1.5e-09 Pumilio-family RNA binding repeat
PUF PF00806 375 - 405 2.4e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1279
AatII GACGTC 2 cut(s) 35, 880
AccB1I GGYRCC 3 cut(s) 125, 148, 1069
AccI GTMKAC 1 cut(s) 453
AciI CCGC 3 cut(s) 110, 113, 169
AclWI GGATC 1 cut(s) 930
AcyI GRCGYC 4 cut(s) 32, 126, 149, 877
AfiI CCNNNNNNNGG 2 cut(s) 122, 919
AgsI TTSAA 9 cut(s) 103, 227, 443, 631, 742, 1007, 1106, 1233, 1328
AjnI CCWGG 1 cut(s) 912
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AluBI AGCT 6 cut(s) 71, 324, 401, 508, 681, 1343
AluI AGCT 6 cut(s) 71, 324, 401, 508, 681, 1343
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 3 cut(s) 97, 1090, 1301
AlwI GGATC 1 cut(s) 930
AlwNI CAGNNNCTG 2 cut(s) 251, 547
Ama87I CYCGRG 2 cut(s) 645, 1058
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 4 cut(s) 71, 887, 1045, 1131
AseI ATTAAT 1 cut(s) 908
Asp700I GAANNNNTTC 2 cut(s) 422, 569
AspLEI GCGC 3 cut(s) 128, 151, 919
AspS9I GGNCC 5 cut(s) 303, 370, 463, 478, 558
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 796, 1291
AvaI CYCGRG 2 cut(s) 645, 1058
AvaII GGWCC 4 cut(s) 303, 370, 478, 558
BaeGI GKGCMC 1 cut(s) 1072
BanI GGYRCC 3 cut(s) 125, 148, 1069
BbsI GAAGAC 2 cut(s) 764, 1258
Bbv12I GWGCWC 1 cut(s) 676
BbvI GCAGC 4 cut(s) 58, 874, 1032, 1143
BccI CCATC 3 cut(s) 169, 191, 301
BceAI ACGGC 3 cut(s) 100, 585, 1290
BciT130I CCWGG 1 cut(s) 914
BclI TGATCA 3 cut(s) 55, 253, 975
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 3 cut(s) 97, 1090, 1301
BfaI CTAG 2 cut(s) 65, 279
BfmI CTRYAG 3 cut(s) 836, 1129, 1191
BfoI RGCGCY 2 cut(s) 129, 152
BisI GCNGC 7 cut(s) 72, 110, 113, 169, 888, 1046, 1132
BlpI GCTNAGC 1 cut(s) 1349
BlsI GCNGC 7 cut(s) 73, 111, 114, 170, 889, 1047, 1133
Bme1390I CCNGG 2 cut(s) 186, 914
Bme18I GGWCC 4 cut(s) 303, 370, 478, 558
BmeT110I CYCGRG 2 cut(s) 645, 1058
BmgT120I GGNCC 5 cut(s) 303, 370, 463, 478, 558
BmiI GGNNCC 5 cut(s) 127, 150, 305, 480, 1071
BmrFI CCNGG 2 cut(s) 186, 914
BmsI GCATC 2 cut(s) 892, 1255
BpiI GAAGAC 2 cut(s) 764, 1258
BplI GAGNNNNNCTC 2 cut(s) 641, 673
Bpu10I CCTNAGC 2 cut(s) 262, 1031
Bpu1102I GCTNAGC 1 cut(s) 1349
BpuMI CCSGG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 4 cut(s) 32, 126, 149, 877
BsaI GGTCTC 1 cut(s) 1301
BsaJI CCNNGG 3 cut(s) 366, 601, 1290
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 919
Bse3DI GCAATG 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 1 cut(s) 914
BseDI CCNNGG 3 cut(s) 366, 601, 1290
BseGI GGATG 4 cut(s) 95, 415, 760, 1246
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 122, 919
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 5 cut(s) 258, 689, 1085, 1307, 1363
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 1072
BseXI GCAGC 4 cut(s) 58, 874, 1032, 1143
BseYI CCCAGC 2 cut(s) 234, 1339
BshFI GGCC 1 cut(s) 464
BshNI GGYRCC 3 cut(s) 125, 148, 1069
BsiHKAI GWGCWC 1 cut(s) 676
BsiHKCI CYCGRG 2 cut(s) 645, 1058
BsiSI CCGG 2 cut(s) 123, 185
BslFI GGGAC 2 cut(s) 289, 619
BslI CCNNNNNNNGG 2 cut(s) 122, 919
BsmAI GTCTC 3 cut(s) 97, 1090, 1301
BsmFI GGGAC 2 cut(s) 289, 619
BsnI GGCC 1 cut(s) 464
Bso31I GGTCTC 1 cut(s) 1301
BsoBI CYCGRG 2 cut(s) 645, 1058
Bsp1286I GDGCHC 2 cut(s) 676, 1072
Bsp143I GATC 5 cut(s) 55, 253, 786, 922, 975
Bsp1720I GCTNAGC 1 cut(s) 1349
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 3 cut(s) 110, 113, 169
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 5 cut(s) 257, 690, 1086, 1308, 1362
BspHI TCATGA 1 cut(s) 978
BspLI GGNNCC 5 cut(s) 127, 150, 305, 480, 1071
BspMAI CTGCAG 2 cut(s) 1133, 1195
BspPI GGATC 1 cut(s) 930
BspQI GCTCTTC 1 cut(s) 1176
BspT107I GGYRCC 3 cut(s) 125, 148, 1069
BspTNI GGTCTC 1 cut(s) 1301
BsrDI GCAATG 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 366, 601, 1290
BssMI GATC 5 cut(s) 55, 253, 786, 922, 975
BssNI GRCGYC 4 cut(s) 32, 126, 149, 877
BssT1I CCWWGG 2 cut(s) 366, 1290
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 5 cut(s) 137, 627, 797, 857, 1101
Bst6I CTCTTC 2 cut(s) 438, 1176
BstACI GRCGYC 4 cut(s) 32, 126, 149, 877
BstC8I GCNNGC 2 cut(s) 833, 1377
BstDEI CTNAG 8 cut(s) 244, 262, 677, 698, 1031, 1094, 1316, 1349
BstDSI CCRYGG 2 cut(s) 366, 601
BstENI CCTNNNNNAGG 1 cut(s) 917
BstF5I GGATG 4 cut(s) 95, 415, 760, 1246
BstH2I RGCGCY 2 cut(s) 129, 152
BstHHI GCGC 3 cut(s) 128, 151, 919
BstKTI GATC 5 cut(s) 58, 256, 789, 925, 978
BstMAI GTCTC 3 cut(s) 97, 1090, 1301
BstMBI GATC 5 cut(s) 55, 253, 786, 922, 975
BstMWI GCNNNNNNNGC 4 cut(s) 240, 597, 1243, 1381
BstNI CCWGG 1 cut(s) 914
BstSCI CCNGG 2 cut(s) 184, 912
BstSFI CTRYAG 3 cut(s) 836, 1129, 1191
BstSLI GKGCMC 1 cut(s) 1072
BstV1I GCAGC 4 cut(s) 58, 874, 1032, 1143
BstV2I GAAGAC 2 cut(s) 764, 1258
BstXI CCANNNNNNTGG 1 cut(s) 300
BsuRI GGCC 1 cut(s) 464
BtgI CCRYGG 2 cut(s) 366, 601
BtsCI GGATG 4 cut(s) 95, 415, 760, 1246
BtsI GCAGTG 1 cut(s) 848
BtsIMutI CAGTG 2 cut(s) 426, 848
Cac8I GCNNGC 2 cut(s) 833, 1377
CaiI CAGNNNCTG 2 cut(s) 251, 547
CciI TCATGA 1 cut(s) 978
CfoI GCGC 3 cut(s) 128, 151, 919
Cfr13I GGNCC 5 cut(s) 303, 370, 463, 478, 558
CviAII CATG 7 cut(s) 53, 181, 257, 367, 458, 727, 979
DdeI CTNAG 8 cut(s) 244, 262, 677, 698, 1031, 1094, 1316, 1349
DinI GGCGCC 2 cut(s) 127, 150
DpnI GATC 5 cut(s) 57, 255, 788, 924, 977
DpnII GATC 5 cut(s) 55, 253, 786, 922, 975
DrdI GACNNNNNNGTC 1 cut(s) 1279
DseDI GACNNNNNNGTC 1 cut(s) 1279
Eam1104I CTCTTC 2 cut(s) 438, 1176
EarI CTCTTC 2 cut(s) 438, 1176
Eco130I CCWWGG 2 cut(s) 366, 1290
Eco31I GGTCTC 1 cut(s) 1301
Eco47I GGWCC 4 cut(s) 303, 370, 478, 558
Eco88I CYCGRG 2 cut(s) 645, 1058
EcoNI CCTNNNNNAGG 1 cut(s) 917
EcoRII CCWGG 1 cut(s) 912
EcoT14I CCWWGG 2 cut(s) 366, 1290
EcoT22I ATGCAT 2 cut(s) 907, 1248
EgeI GGCGCC 2 cut(s) 127, 150
EheI GGCGCC 2 cut(s) 127, 150
ErhI CCWWGG 2 cut(s) 366, 1290
FaeI CATG 7 cut(s) 56, 184, 260, 370, 461, 730, 982
FaqI GGGAC 2 cut(s) 289, 619
FatI CATG 7 cut(s) 52, 180, 256, 366, 457, 726, 978
FbaI TGATCA 3 cut(s) 55, 253, 975
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 7 cut(s) 72, 110, 113, 169, 888, 1046, 1132
FokI GGATG 4 cut(s) 82, 422, 767, 1233
Fsp4HI GCNGC 7 cut(s) 72, 110, 113, 169, 888, 1046, 1132
FspBI CTAG 2 cut(s) 65, 279
GlaI GCGC 3 cut(s) 127, 150, 918
GluI GCNGC 7 cut(s) 72, 110, 113, 169, 888, 1046, 1132
GsaI CCCAGC 2 cut(s) 238, 1343
HaeII RGCGCY 2 cut(s) 129, 152
HaeIII GGCC 1 cut(s) 464
HapII CCGG 2 cut(s) 123, 185
HhaI GCGC 3 cut(s) 128, 151, 919
Hin1I GRCGYC 4 cut(s) 32, 126, 149, 877
Hin1II CATG 7 cut(s) 56, 184, 260, 370, 461, 730, 982
Hin6I GCGC 3 cut(s) 126, 149, 917
HinP1I GCGC 3 cut(s) 126, 149, 917
HincII GTYRAC 2 cut(s) 694, 719
HindII GTYRAC 2 cut(s) 694, 719
HindIII AAGCTT 1 cut(s) 506
HinfI GANTC 6 cut(s) 472, 544, 565, 607, 1172, 1318
HpaII CCGG 2 cut(s) 123, 185
HphI GGTGA 2 cut(s) 796, 1291
Hpy166II GTNNAC 6 cut(s) 284, 426, 454, 558, 694, 719
Hpy188III TCNNGA 5 cut(s) 224, 476, 979, 1176, 1270
Hpy8I GTNNAC 6 cut(s) 284, 426, 454, 558, 694, 719
Hpy99I CGWCG 3 cut(s) 34, 37, 1404
HpyAV CCTTC 2 cut(s) 109, 602
HpyCH4III ACNGT 5 cut(s) 137, 627, 797, 857, 1101
HpyCH4IV ACGT 3 cut(s) 32, 877, 1402
HpyCH4V TGCA 7 cut(s) 809, 905, 969, 1048, 1131, 1193, 1246
HpyF10VI GCNNNNNNNGC 4 cut(s) 240, 597, 1243, 1381
HpyF3I CTNAG 8 cut(s) 244, 262, 677, 698, 1031, 1094, 1316, 1349
HpySE526I ACGT 3 cut(s) 32, 877, 1402
Hsp92I GRCGYC 4 cut(s) 32, 126, 149, 877
Hsp92II CATG 7 cut(s) 56, 184, 260, 370, 461, 730, 982
HspAI GCGC 3 cut(s) 126, 149, 917
KasI GGCGCC 2 cut(s) 125, 148
Ksp22I TGATCA 3 cut(s) 55, 253, 975
Kzo9I GATC 5 cut(s) 55, 253, 786, 922, 975
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 4 cut(s) 58, 874, 1032, 1143
LweI GCATC 2 cut(s) 892, 1255
MaeI CTAG 2 cut(s) 65, 279
MaeII ACGT 3 cut(s) 32, 877, 1402
MaeIII GTNAC 1 cut(s) 1078
MalI GATC 5 cut(s) 57, 255, 788, 924, 977
MboI GATC 5 cut(s) 55, 253, 786, 922, 975
MboII GAAGA 9 cut(s) 239, 455, 643, 754, 764, 911, 1082, 1193, 1258
MhlI GDGCHC 2 cut(s) 676, 1072
MluCI AATT 6 cut(s) 804, 882, 1002, 1111, 1197, 1258
Mly113I GGCGCC 2 cut(s) 126, 149
MlyI GAGTC 3 cut(s) 601, 1181, 1327
MnlI CCTC 6 cut(s) 400, 439, 508, 520, 528, 1059
Mph1103I ATGCAT 2 cut(s) 907, 1248
MroXI GAANNNNTTC 2 cut(s) 422, 569
MseI TTAA 3 cut(s) 551, 908, 1001
MslI CAYNNNNRTG 2 cut(s) 431, 600
MspI CCGG 2 cut(s) 123, 185
MspR9I CCNGG 2 cut(s) 186, 914
MvaI CCWGG 1 cut(s) 914
MwoI GCNNNNNNNGC 4 cut(s) 240, 597, 1243, 1381
NarI GGCGCC 2 cut(s) 126, 149
NciI CCSGG 1 cut(s) 186
NcoI CCATGG 1 cut(s) 366
NdeII GATC 5 cut(s) 55, 253, 786, 922, 975
NlaIII CATG 7 cut(s) 56, 184, 260, 370, 461, 730, 982
NlaIV GGNNCC 5 cut(s) 127, 150, 305, 480, 1071
NmuCI GTSAC 1 cut(s) 1078
NsiI ATGCAT 2 cut(s) 907, 1248
PagI TCATGA 1 cut(s) 978
PciSI GCTCTTC 1 cut(s) 1176
PdmI GAANNNNTTC 2 cut(s) 422, 569
PfeI GAWTC 3 cut(s) 472, 544, 565
PkrI GCNGC 7 cut(s) 73, 111, 114, 170, 889, 1047, 1133
PleI GAGTC 3 cut(s) 601, 1180, 1326
PluTI GGCGCC 2 cut(s) 129, 152
PpsI GAGTC 3 cut(s) 601, 1180, 1326
PshBI ATTAAT 1 cut(s) 908
Psp6I CCWGG 1 cut(s) 912
PspFI CCCAGC 2 cut(s) 234, 1339
PspGI CCWGG 1 cut(s) 912
PspN4I GGNNCC 5 cut(s) 127, 150, 305, 480, 1071
PspPI GGNCC 5 cut(s) 303, 370, 463, 478, 558
PstI CTGCAG 2 cut(s) 1133, 1195
PstNI CAGNNNCTG 2 cut(s) 251, 547
RseI CAYNNNNRTG 2 cut(s) 431, 600
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 3 cut(s) 551, 908, 1001
SatI GCNGC 7 cut(s) 72, 110, 113, 169, 888, 1046, 1132
Sau3AI GATC 5 cut(s) 55, 253, 786, 922, 975
Sau96I GGNCC 5 cut(s) 303, 370, 463, 478, 558
SchI GAGTC 3 cut(s) 601, 1181, 1327
ScrFI CCNGG 2 cut(s) 186, 914
SduI GDGCHC 2 cut(s) 676, 1072
SfaNI GCATC 2 cut(s) 892, 1255
SfcI CTRYAG 3 cut(s) 836, 1129, 1191
SfoI GGCGCC 2 cut(s) 127, 150
SinI GGWCC 4 cut(s) 303, 370, 478, 558
SmiMI CAYNNNNRTG 2 cut(s) 431, 600
Sse9I AATT 6 cut(s) 804, 882, 1002, 1111, 1197, 1258
SsiI CCGC 3 cut(s) 110, 113, 169
SspDI GGCGCC 2 cut(s) 125, 148
SspI AATATT 1 cut(s) 734
SspMI CTAG 2 cut(s) 65, 279
StyD4I CCNGG 2 cut(s) 184, 912
StyI CCWWGG 2 cut(s) 366, 1290
TaaI ACNGT 5 cut(s) 137, 627, 797, 857, 1101
TaiI ACGT 3 cut(s) 35, 880, 1405
TaqI TCGA 1 cut(s) 789
TaqII GACCGA 1 cut(s) 200
TasI AATT 6 cut(s) 804, 882, 1002, 1111, 1197, 1258
TauI GCSGC 3 cut(s) 112, 115, 171
TfiI GAWTC 3 cut(s) 472, 544, 565
Tru1I TTAA 3 cut(s) 551, 908, 1001
Tru9I TTAA 3 cut(s) 551, 908, 1001
TscAI CASTG 2 cut(s) 433, 848
TseFI GTSAC 1 cut(s) 1078
TseI GCWGC 4 cut(s) 71, 887, 1045, 1131
Tsp45I GTSAC 1 cut(s) 1078
TspDTI ATGAA 6 cut(s) 189, 584, 719, 771, 959, 1014
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 2 cut(s) 433, 848
VpaK11BI GGWCC 4 cut(s) 303, 370, 478, 558
VspI ATTAAT 1 cut(s) 908
XagI CCTNNNNNAGG 1 cut(s) 917
XmiI GTMKAC 1 cut(s) 453
XmnI GAANNNNTTC 2 cut(s) 422, 569
XspI CTAG 2 cut(s) 65, 279
ZraI GACGTC 2 cut(s) 33, 878
Zsp2I ATGCAT 2 cut(s) 907, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.