Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0009606

Physical Location & Seq

Genomic Coordinates Reverse (-)

25083139 .. 25086844

Gene Structure 3706 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g33440.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGGAGGGTTTCGGCTACTTCACCGATACGACGTCGCAGACCCCTTCTTCTTATTCCGATCAATCCCTAGAATCCGCTTTCTCTCGCCTCAACGTCTCCTCCTTCAGCCAGCCGCCGTTTTCCGGCGTCGGTTACAGCTCCGGTCCGGCGCCGAAATACCCATCACGCCGCAATGAGCCCACTTTTCTTGATGATGGGGTTCTTGGTCGTTTGGGTCTAGAAGACTGCTGGGTTGGTGGCTCTGGTACTGATCATGGCTTTGGTGGGTTTGGTTCGAATTCAAATTCGAATTTACACTCTGACATTGCCACAAATTGGTCCCAAGAACCACTGAACAGCTTGTCTGTGGCGGATTTGCGAGGAAAGATTGCGTCTTTGGCGAAGGATCAATACGGGTGTCGGTTCTTTCAGAAAGCCATGGATGATGAGCCCAAGGAGTTTACAGATATGATATTTGCAGAGGTGGTGGAGAATGTGGGGCATTTGATGCTCGACCCGTATGGAAATTATGTGGTGCAGAAGCTTGTGGAGGTCTGCAGTGAGGAGCAGAGGACCCAGATTCTGTTGAGGTTGACCAAGAATCCGTTTCTGTTCATCACTCTCTGCCTTCATAGCCGTGGGACTCGGTCTGTTCAGAAACTGATGAAGAAGATCAGTACCCGAGATCAAATTTCTTTGTTTGTGAGTGCACTAAGCTGTGGTGTGGTTGACCTCAGTATGAACATAAATGGTCAACACATTATAGAATACTGCCTGAAGAACTTCGCAGACAAACACAAAAGGTGTCTTCTGAACGTGATGATTGACAACTGTATTAGCATTGCAAAAGATAAGGTCGGTTGTTGCGTGCTACAACAGTGTATCGATTACTGTTATGGAGAAACAAGAGGACGTTTGATTGCTGCTATAATTGGAGAAGCATTAAGCCTAGCACAAGATCGTTATGGAAACTATGTGCTGCAACATTTACTTGGGTTAAAGACACCTCAAGTCACATCATCTCTTCTGAGACAGTTTCAAGGGAATTATATGTTTCTCTCCTGCGGCAAATATGGTAGTAATGTTGTGGAGAAGTGCTTGTTAGAGTCAGAAGAGGAACAGTCTGCCGATATTATTAGAGAGTTGTGTATAAGTCCGAACCTTTCAATGCTTCTTATGCATCCTTTTGGAAACTATGTCTTCCAGACCGCACTGTCAGTTTCTAAGGGTCTCGCCCATGCTTTTCTCTTAAATCTGGTTCAACAATACACTCCTGCTTTGCTCAGCAATCAATATGGAAAAAGGGTGCTTGCTTGGCGTGAAAGGAGCTTGCGACGTATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUF

Protein Analysis

441

Amino Acids

49.07

Weight (kDa)

6.75

Isoelectric Point (pI)

39.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	123 - 155	6.7e-08	Pumilio-family RNA binding repeat
PUF	PF00806	158 - 189	3.3e-11	Pumilio-family RNA binding repeat
PUF	PF00806	270 - 304	7e-11	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08840
fragaria_vesca	FvH4_2g33440 FvH4_2g33440 FvH4_2g33440
malus_domestica	MD08G1089500.v1.1 MD15G1074100.v1.1
prunus_persica	Prupe.1G426900_v2.0.a1 Prupe.1G426900_v2.0.a1 Prupe.1G426900_v2.0.a1
pyrus_communis	pycom08g07280 pycom15g06930
rosa_chinensis	RchiOBHm_Chr6g0309091
rosa_laevigata	RLG00000010590
rosa_multiflora	Rmu_sc0012331.1_g000001
rosa_roxburghii	Rroxscaffold_1G00037870 Rroxscaffold_2G00124690
rosa_rugosa	Rorug06G0369700
rosa_samantha	Rh6AG482400 Rh6BG491400 Rh6CG496200 Rh6DG483100
rosa_wichuraiana	Rw6G042000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1192
AatII	GACGTC	1 cut(s)	35
AccB1I	GGYRCC	1 cut(s)	148
AccB7I	CCANNNNNTGG	1 cut(s)	315
AciI	CCGC	6 cut(s)	75, 113, 169, 350, 1044, 1188
AclWI	GGATC	1 cut(s)	393
AcsI	RAATTY	4 cut(s)	277, 283, 289, 669
AcuI	CTGAAG	2 cut(s)	88, 776
AcyI	GRCGYC	3 cut(s)	32, 126, 149
AfaI	GTAC	2 cut(s)	247, 658
AfiI	CCNNNNNNNGG	2 cut(s)	122, 315
AgsI	TTSAA	4 cut(s)	282, 1019, 1146, 1241
AluBI	AGCT	5 cut(s)	138, 339, 523, 696, 1308
AluI	AGCT	5 cut(s)	138, 339, 523, 696, 1308
Alw21I	GWGCWC	1 cut(s)	691
Alw26I	GTCTC	3 cut(s)	100, 1003, 1214
Alw44I	GTGCAC	1 cut(s)	687
AlwI	GGATC	1 cut(s)	393
AlwNI	CAGNNNCTG	2 cut(s)	562, 640
Ama87I	CYCGRG	1 cut(s)	660
ApaLI	GTGCAC	1 cut(s)	687
ApeKI	GCWGC	2 cut(s)	902, 958
ApoI	RAATTY	4 cut(s)	277, 283, 289, 669
Asp700I	GAANNNNTTC	1 cut(s)	761
AspLEI	GCGC	1 cut(s)	151
AspS9I	GGNCC	3 cut(s)	143, 318, 552
AsuHPI	GGTGA	1 cut(s)	13
AsuII	TTCGAA	2 cut(s)	275, 287
AvaI	CYCGRG	1 cut(s)	660
AvaII	GGWCC	3 cut(s)	143, 318, 552
BaeGI	GKGCMC	1 cut(s)	691
BanI	GGYRCC	1 cut(s)	148
BanII	GRGCYC	2 cut(s)	180, 432
BarI	GAAGNNNNNNTAC	2 cut(s)	374, 406
BbsI	GAAGAC	3 cut(s)	228, 779, 1171
Bbv12I	GWGCWC	1 cut(s)	691
BbvI	GCAGC	2 cut(s)	889, 945
BccI	CCATC	2 cut(s)	169, 188
BceAI	ACGGC	2 cut(s)	100, 600
BclI	TGATCA	1 cut(s)	250
BcoDI	GTCTC	3 cut(s)	100, 1003, 1214
BfaI	CTAG	3 cut(s)	68, 218, 929
BfmI	CTRYAG	1 cut(s)	535
BfoI	RGCGCY	1 cut(s)	152
BisI	GCNGC	5 cut(s)	113, 169, 903, 959, 1045
BlpI	GCTNAGC	1 cut(s)	1262
BlsI	GCNGC	5 cut(s)	114, 170, 904, 960, 1046
Bme18I	GGWCC	3 cut(s)	143, 318, 552
BmeT110I	CYCGRG	1 cut(s)	660
BmgT120I	GGNCC	3 cut(s)	143, 318, 552
BmiI	GGNNCC	3 cut(s)	150, 320, 554
BmsI	GCATC	2 cut(s)	477, 1168
BpiI	GAAGAC	3 cut(s)	228, 779, 1171
Bpu1102I	GCTNAGC	1 cut(s)	1262
Bpu14I	TTCGAA	2 cut(s)	275, 287
BpuEI	CTTGAG	1 cut(s)	972
Bsa29I	ATCGAT	1 cut(s)	864
BsaHI	GRCGYC	3 cut(s)	32, 126, 149
BsaI	GGTCTC	1 cut(s)	1214
BsaJI	CCNNGG	3 cut(s)	417, 432, 616
BsaWI	WCCGGW	1 cut(s)	140
BsaXI	ACNNNNNCTCC	2 cut(s)	83, 113
Bsc4I	CCNNNNNNNGG	2 cut(s)	122, 315
Bse3DI	GCAATG	3 cut(s)	178, 303, 819
BseCI	ATCGAT	1 cut(s)	864
BseDI	CCNNGG	3 cut(s)	417, 432, 616
BseGI	GGATG	2 cut(s)	427, 1159
BseLI	CCNNNNNNNGG	2 cut(s)	122, 315
BseMI	GCAATG	3 cut(s)	178, 303, 819
BseMII	CTCAG	3 cut(s)	727, 998, 1276
BseRI	GAGGAG	2 cut(s)	88, 557
BseSI	GKGCMC	1 cut(s)	691
BseXI	GCAGC	2 cut(s)	889, 945
BseYI	CCCAGC	1 cut(s)	228
BsgI	GTGCAG	1 cut(s)	536
BshNI	GGYRCC	1 cut(s)	148
BshVI	ATCGAT	1 cut(s)	864
BsiHKAI	GWGCWC	1 cut(s)	691
BsiHKCI	CYCGRG	1 cut(s)	660
BsiSI	CCGG	3 cut(s)	123, 141, 146
BslFI	GGGAC	2 cut(s)	304, 634
BslI	CCNNNNNNNGG	2 cut(s)	122, 315
BsmAI	GTCTC	3 cut(s)	100, 1003, 1214
BsmBI	CGTCTC	1 cut(s)	100
BsmFI	GGGAC	2 cut(s)	304, 634
Bso31I	GGTCTC	1 cut(s)	1214
BsoBI	CYCGRG	1 cut(s)	660
Bsp119I	TTCGAA	2 cut(s)	275, 287
Bsp1286I	GDGCHC	3 cut(s)	180, 432, 691
Bsp143I	GATC	6 cut(s)	58, 250, 385, 651, 664, 937
Bsp1720I	GCTNAGC	1 cut(s)	1262
Bsp19I	CCATGG	1 cut(s)	417
BspACI	CCGC	6 cut(s)	75, 113, 169, 350, 1044, 1188
BspCNI	CTCAG	3 cut(s)	726, 999, 1275
BspDI	ATCGAT	1 cut(s)	864
BspLI	GGNNCC	3 cut(s)	150, 320, 554
BspMAI	CTGCAG	1 cut(s)	539
BspPI	GGATC	1 cut(s)	393
BspT104I	TTCGAA	2 cut(s)	275, 287
BspT107I	GGYRCC	1 cut(s)	148
BspTNI	GGTCTC	1 cut(s)	1214
BsrDI	GCAATG	3 cut(s)	178, 303, 819
BssECI	CCNNGG	3 cut(s)	417, 432, 616
BssMI	GATC	6 cut(s)	58, 250, 385, 651, 664, 937
BssNI	GRCGYC	3 cut(s)	32, 126, 149
BssT1I	CCWWGG	2 cut(s)	417, 432
Bst4CI	ACNGT	6 cut(s)	812, 858, 872, 1014, 1101, 1194
Bst6I	CTCTTC	2 cut(s)	1008, 1086
BstACI	GRCGYC	3 cut(s)	32, 126, 149
BstAPI	GCANNNNNTGC	1 cut(s)	487
BstBI	TTCGAA	2 cut(s)	275, 287
BstC8I	GCNNGC	4 cut(s)	110, 848, 1290, 1310
BstDEI	CTNAG	5 cut(s)	692, 713, 1007, 1203, 1262
BstDSI	CCRYGG	2 cut(s)	417, 616
BstF5I	GGATG	2 cut(s)	427, 1159
BstH2I	RGCGCY	1 cut(s)	152
BstHHI	GCGC	1 cut(s)	151
BstKTI	GATC	6 cut(s)	61, 253, 388, 654, 667, 940
BstMAI	GTCTC	3 cut(s)	100, 1003, 1214
BstMBI	GATC	6 cut(s)	58, 250, 385, 651, 664, 937
BstMWI	GCNNNNNNNGC	5 cut(s)	377, 487, 612, 1156, 1294
BstSFI	CTRYAG	1 cut(s)	535
BstSLI	GKGCMC	1 cut(s)	691
BstV1I	GCAGC	2 cut(s)	889, 945
BstV2I	GAAGAC	3 cut(s)	228, 779, 1171
Bsu15I	ATCGAT	1 cut(s)	864
BsuTUI	ATCGAT	1 cut(s)	864
BtgI	CCRYGG	2 cut(s)	417, 616
BtsCI	GGATG	2 cut(s)	427, 1159
BtsI	GCAGTG	1 cut(s)	544
BtsIMutI	CAGTG	4 cut(s)	329, 544, 863, 1190
Cac8I	GCNNGC	4 cut(s)	110, 848, 1290, 1310
CaiI	CAGNNNCTG	2 cut(s)	562, 640
CfoI	GCGC	1 cut(s)	151
Cfr13I	GGNCC	3 cut(s)	143, 318, 552
ClaI	ATCGAT	1 cut(s)	864
CpoI	CGGWCCG	1 cut(s)	143
CseI	GACGC	2 cut(s)	115, 360
Csp6I	GTAC	2 cut(s)	246, 657
CspCI	CAANNNNNGTGG	2 cut(s)	169, 204
CspI	CGGWCCG	1 cut(s)	143
CviAII	CATG	3 cut(s)	254, 418, 1217
CviQI	GTAC	2 cut(s)	246, 657
DdeI	CTNAG	5 cut(s)	692, 713, 1007, 1203, 1262
DinI	GGCGCC	1 cut(s)	150
DpnI	GATC	6 cut(s)	60, 252, 387, 653, 666, 939
DpnII	GATC	6 cut(s)	58, 250, 385, 651, 664, 937
DrdI	GACNNNNNNGTC	1 cut(s)	1192
DseDI	GACNNNNNNGTC	1 cut(s)	1192
Eam1104I	CTCTTC	2 cut(s)	1008, 1086
EarI	CTCTTC	2 cut(s)	1008, 1086
EciI	GGCGGA	1 cut(s)	365
Eco130I	CCWWGG	2 cut(s)	417, 432
Eco24I	GRGCYC	2 cut(s)	180, 432
Eco31I	GGTCTC	1 cut(s)	1214
Eco47I	GGWCC	3 cut(s)	143, 318, 552
Eco57I	CTGAAG	2 cut(s)	88, 776
Eco88I	CYCGRG	1 cut(s)	660
EcoO109I	RGGNCCY	1 cut(s)	552
EcoRI	GAATTC	1 cut(s)	277
EcoT14I	CCWWGG	2 cut(s)	417, 432
EcoT22I	ATGCAT	1 cut(s)	1161
EcoT38I	GRGCYC	2 cut(s)	180, 432
EgeI	GGCGCC	1 cut(s)	150
EheI	GGCGCC	1 cut(s)	150
ErhI	CCWWGG	2 cut(s)	417, 432
Esp3I	CGTCTC	1 cut(s)	100
FaeI	CATG	3 cut(s)	257, 421, 1220
FaqI	GGGAC	2 cut(s)	304, 634
FatI	CATG	3 cut(s)	253, 417, 1216
FbaI	TGATCA	1 cut(s)	250
Fnu4HI	GCNGC	5 cut(s)	113, 169, 903, 959, 1045
FokI	GGATG	2 cut(s)	434, 1146
FriOI	GRGCYC	2 cut(s)	180, 432
Fsp4HI	GCNGC	5 cut(s)	113, 169, 903, 959, 1045
FspBI	CTAG	3 cut(s)	68, 218, 929
GlaI	GCGC	1 cut(s)	150
GluI	GCNGC	5 cut(s)	113, 169, 903, 959, 1045
GsaI	CCCAGC	1 cut(s)	232
HaeII	RGCGCY	1 cut(s)	152
HapII	CCGG	3 cut(s)	123, 141, 146
HgaI	GACGC	2 cut(s)	115, 360
HhaI	GCGC	1 cut(s)	151
Hin1I	GRCGYC	3 cut(s)	32, 126, 149
Hin1II	CATG	3 cut(s)	257, 421, 1220
Hin6I	GCGC	1 cut(s)	149
HinP1I	GCGC	1 cut(s)	149
HincII	GTYRAC	3 cut(s)	573, 709, 734
HindII	GTYRAC	3 cut(s)	573, 709, 734
HindIII	AAGCTT	1 cut(s)	521
HinfI	GANTC	5 cut(s)	71, 559, 580, 622, 1085
HpaII	CCGG	3 cut(s)	123, 141, 146
HphI	GGTGA	1 cut(s)	13
Hpy166II	GTNNAC	5 cut(s)	441, 573, 689, 709, 734
Hpy188I	TCNGA	8 cut(s)	58, 301, 411, 636, 792, 1008, 1090, 1137
Hpy188III	TCNNGA	3 cut(s)	188, 218, 1183
Hpy8I	GTNNAC	5 cut(s)	441, 573, 689, 709, 734
Hpy99I	CGWCG	4 cut(s)	34, 37, 131, 1317
HpyAV	CCTTC	4 cut(s)	54, 112, 376, 617
HpyCH4III	ACNGT	6 cut(s)	812, 858, 872, 1014, 1101, 1194
HpyCH4IV	ACGT	5 cut(s)	32, 93, 795, 892, 1315
HpyCH4V	TGCA	7 cut(s)	458, 517, 537, 689, 824, 961, 1159
HpyF10VI	GCNNNNNNNGC	5 cut(s)	377, 487, 612, 1156, 1294
HpyF3I	CTNAG	5 cut(s)	692, 713, 1007, 1203, 1262
HpySE526I	ACGT	5 cut(s)	32, 93, 795, 892, 1315
Hsp92I	GRCGYC	3 cut(s)	32, 126, 149
Hsp92II	CATG	3 cut(s)	257, 421, 1220
HspAI	GCGC	1 cut(s)	149
KasI	GGCGCC	1 cut(s)	148
Ksp22I	TGATCA	1 cut(s)	250
Kzo9I	GATC	6 cut(s)	58, 250, 385, 651, 664, 937
LmnI	GCTCC	3 cut(s)	143, 544, 1305
Lsp1109I	GCAGC	2 cut(s)	889, 945
LweI	GCATC	2 cut(s)	477, 1168
MaeI	CTAG	3 cut(s)	68, 218, 929
MaeII	ACGT	5 cut(s)	32, 93, 795, 892, 1315
MaeIII	GTNAC	2 cut(s)	131, 991
MalI	GATC	6 cut(s)	60, 252, 387, 653, 666, 939
MboI	GATC	6 cut(s)	58, 250, 385, 651, 664, 937
MboII	GAAGA	9 cut(s)	39, 233, 658, 661, 769, 779, 995, 1103, 1171
MhlI	GDGCHC	3 cut(s)	180, 432, 691
MluCI	AATT	8 cut(s)	277, 283, 289, 313, 505, 669, 909, 1024
Mly113I	GGCGCC	1 cut(s)	149
MlyI	GAGTC	2 cut(s)	616, 1094
Mph1103I	ATGCAT	1 cut(s)	1161
MroXI	GAANNNNTTC	1 cut(s)	761
MseI	TTAA	3 cut(s)	923, 977, 1229
MslI	CAYNNNNRTG	1 cut(s)	615
MspI	CCGG	3 cut(s)	123, 141, 146
MwoI	GCNNNNNNNGC	5 cut(s)	377, 487, 612, 1156, 1294
NarI	GGCGCC	1 cut(s)	149
NcoI	CCATGG	1 cut(s)	417
NdeII	GATC	6 cut(s)	58, 250, 385, 651, 664, 937
NlaIII	CATG	3 cut(s)	257, 421, 1220
NlaIV	GGNNCC	3 cut(s)	150, 320, 554
NmuCI	GTSAC	1 cut(s)	991
NsiI	ATGCAT	1 cut(s)	1161
NspV	TTCGAA	2 cut(s)	275, 287
PcsI	WCGNNNNNNNCGW	1 cut(s)	843
PdmI	GAANNNNTTC	1 cut(s)	761
PfeI	GAWTC	3 cut(s)	71, 559, 580
PflFI	GACNNNGTC	1 cut(s)	625
PflMI	CCANNNNNTGG	1 cut(s)	315
PkrI	GCNGC	5 cut(s)	114, 170, 904, 960, 1046
PleI	GAGTC	2 cut(s)	616, 1093
PluTI	GGCGCC	1 cut(s)	152
PpsI	GAGTC	2 cut(s)	616, 1093
PpuMI	RGGWCCY	1 cut(s)	552
Psp5II	RGGWCCY	1 cut(s)	552
PspFI	CCCAGC	1 cut(s)	228
PspN4I	GGNNCC	3 cut(s)	150, 320, 554
PspPI	GGNCC	3 cut(s)	143, 318, 552
PspPPI	RGGWCCY	1 cut(s)	552
PstI	CTGCAG	1 cut(s)	539
PstNI	CAGNNNCTG	2 cut(s)	562, 640
PsyI	GACNNNGTC	1 cut(s)	625
RsaI	GTAC	2 cut(s)	247, 658
RsaNI	GTAC	2 cut(s)	246, 657
RseI	CAYNNNNRTG	1 cut(s)	615
Rsr2I	CGGWCCG	1 cut(s)	143
RsrII	CGGWCCG	1 cut(s)	143
SaqAI	TTAA	3 cut(s)	923, 977, 1229
SatI	GCNGC	5 cut(s)	113, 169, 903, 959, 1045
Sau3AI	GATC	6 cut(s)	58, 250, 385, 651, 664, 937
Sau96I	GGNCC	3 cut(s)	143, 318, 552
SchI	GAGTC	2 cut(s)	616, 1094
SduI	GDGCHC	3 cut(s)	180, 432, 691
SfaNI	GCATC	2 cut(s)	477, 1168
SfcI	CTRYAG	1 cut(s)	535
SfoI	GGCGCC	1 cut(s)	150
SfuI	TTCGAA	2 cut(s)	275, 287
SinI	GGWCC	3 cut(s)	143, 318, 552
SmiMI	CAYNNNNRTG	1 cut(s)	615
SmlI	CTYRAG	1 cut(s)	987
SmoI	CTYRAG	1 cut(s)	987
Sse9I	AATT	8 cut(s)	277, 283, 289, 313, 505, 669, 909, 1024
SsiI	CCGC	6 cut(s)	75, 113, 169, 350, 1044, 1188
SspDI	GGCGCC	1 cut(s)	148
SspMI	CTAG	3 cut(s)	68, 218, 929
StyI	CCWWGG	2 cut(s)	417, 432
TaaI	ACNGT	6 cut(s)	812, 858, 872, 1014, 1101, 1194
TaiI	ACGT	5 cut(s)	35, 96, 798, 895, 1318
TaqI	TCGA	4 cut(s)	275, 287, 492, 864
TaqII	GACCGA	1 cut(s)	615
TasI	AATT	8 cut(s)	277, 283, 289, 313, 505, 669, 909, 1024
TauI	GCSGC	3 cut(s)	115, 171, 1047
TfiI	GAWTC	3 cut(s)	71, 559, 580
Tru1I	TTAA	3 cut(s)	923, 977, 1229
Tru9I	TTAA	3 cut(s)	923, 977, 1229
TscAI	CASTG	4 cut(s)	336, 544, 863, 1197
TseFI	GTSAC	1 cut(s)	991
TseI	GCWGC	2 cut(s)	902, 958
Tsp45I	GTSAC	1 cut(s)	991
TspDTI	ATGAA	4 cut(s)	583, 599, 659, 734
TspGWI	ACGGA	1 cut(s)	573
TspRI	CASTG	4 cut(s)	336, 544, 863, 1197
Tth111I	GACNNNGTC	1 cut(s)	625
Van91I	CCANNNNNTGG	1 cut(s)	315
VneI	GTGCAC	1 cut(s)	687
VpaK11BI	GGWCC	3 cut(s)	143, 318, 552
XapI	RAATTY	4 cut(s)	277, 283, 289, 669
XbaI	TCTAGA	1 cut(s)	217
XmnI	GAANNNNTTC	1 cut(s)	761
XspI	CTAG	3 cut(s)	68, 218, 929
ZraI	GACGTC	1 cut(s)	33
Zsp2I	ATGCAT	1 cut(s)	1161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.