Rw6G042000

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009606
Physical Location & Seq
Forward (+)
65629137 .. 65632079
2943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G042000.1

Sequence Viewer

Length: 1308 bp
ATGGAGGGTTTCCGATACTTTACCGATACGACGTCGCAGAACCCTATTCCCTATGATCAATCCCTAGAAGCTGCTTTCTCTCGCCTGAATGTCTCATCCTTCAGCCAGCCGCCGCCGCTTTCCTGCGCCGATAACAGTTCTGGGTCGGCGCCGAAATACCCATCACGCCGCAATGAACTCATGCCGGGTGGTTTTGATGGTGGGGTTTTTGGTCATTTGGGTCTTGAAGATTGCTGGGTTGGCTCAGATACTGATCATGGCTTAGGTGGGTTTGGTTCTAGTTTACATAAACCATCTGATTGGTCCCAAGAACCACTCAACAGCTTATCTGTGGCTGATTTGCGTGGAAAGATTGCTTCTTTGTCCATGGACCAATACGGGTGTCGCTTCTTTCAGAGAGCTATGGAGGATGAGCCGAAAGAGTTCACTGATATGATATTTGAAGAGGTTGTAGACCATGTGGCCCATTTGATTCTGGACCCTTATGGCAACTATGTTGTTCAGAAGCTTGTGGAGGTTTGTAGTGAGGAGCAGAGGACACAGATTCTGTTAATGTGGACCAAGAATCCGTTCCATTTTATTACTCTTTGCCTTCATAGCCGTGGGACTGGCTCTGTTCTGAAACTGTTGAAGAAAATCACTACCCGAGAGCAAATCTCTGTGTTTGTGAGTGCTCTAAGCTGTGGTGCTGTTGACCTGAGTATGAACATCAATGGTCAACATATCATGGAATATTGCTTGAAGAACTTTTCGGATGAACACAAAAGGTGTCTTCTGAATGTGGTGATCGACAACTGTATTGGAATTGCAAAAGATAAGGTTGGTTGTTGCGTGCTACAGCAGTGTGTTGATTACTGTTATGGAGAAACAAAAGGACGTCTAATTGCTGCGATAATAGAAGACGCATTAATCCTGGCGCAGGATCGTTATGGCAACTATGTGCTGCAACATTTACTCGGGTTGAGGGCACCACAAGTCACATCAAATCTTCTGAGACAGTTTCAAGGGAATTATATGTTTCTCTCCTGCAGCAAGTATGGTAGTAATGTTGTGGAGAAGTGCTTGTTAGAGTCGGGAGAAGAGCAGTCTGCAGAAATTATTAGAGAGTTGTGTAGAAGCCCAAATCTTTCAATGCTTCTTATGCATCCTTTCGGAAATTATGTCTTCCAGACGGCATTGTCAGTTTCCAAGGGTCTCACCCATAAGTTTCTGCTGAGTCTGGTTCAACATTACACCCCAGCTCTGCTCAGCAATCAATATGGAAAAAGGGTGCTTGCTTGGCGTGAAAGGAACTTGCGACGTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

435

Amino Acids

48.86

Weight (kDa)

6.24

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 118 - 150 5.2e-08 Pumilio-family RNA binding repeat
PUF PF00806 153 - 183 1.6e-10 Pumilio-family RNA binding repeat
PUF PF00806 265 - 299 1.4e-09 Pumilio-family RNA binding repeat
PUF PF00806 341 - 371 2.2e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1177
AatII GACGTC 2 cut(s) 35, 880
AccB1I GGYRCC 2 cut(s) 148, 967
AccI GTMKAC 1 cut(s) 453
AciI CCGC 4 cut(s) 110, 113, 116, 169
AclWI GGATC 1 cut(s) 930
AcuI CTGAAG 1 cut(s) 85
AcyI GRCGYC 3 cut(s) 32, 149, 877
AfiI CCNNNNNNNGG 1 cut(s) 919
AgsI TTSAA 7 cut(s) 227, 443, 631, 742, 1004, 1131, 1226
AjnI CCWGG 1 cut(s) 912
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AluBI AGCT 6 cut(s) 71, 324, 401, 508, 681, 1241
AluI AGCT 6 cut(s) 71, 324, 401, 508, 681, 1241
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 3 cut(s) 97, 988, 1199
AlwI GGATC 1 cut(s) 930
AlwNI CAGNNNCTG 2 cut(s) 251, 547
Ama87I CYCGRG 2 cut(s) 645, 956
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 4 cut(s) 71, 887, 943, 1029
AseI ATTAAT 1 cut(s) 908
Asp700I GAANNNNTTC 2 cut(s) 422, 569
AspLEI GCGC 3 cut(s) 128, 151, 919
AspS9I GGNCC 5 cut(s) 303, 370, 463, 478, 558
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 796, 1189
AvaI CYCGRG 2 cut(s) 645, 956
AvaII GGWCC 4 cut(s) 303, 370, 478, 558
BaeGI GKGCMC 1 cut(s) 970
BanI GGYRCC 2 cut(s) 148, 967
BbsI GAAGAC 3 cut(s) 764, 906, 1156
Bbv12I GWGCWC 1 cut(s) 676
BbvI GCAGC 4 cut(s) 58, 874, 930, 1041
BccI CCATC 3 cut(s) 169, 191, 301
BceAI ACGGC 2 cut(s) 585, 1188
BciT130I CCWGG 1 cut(s) 914
BclI TGATCA 2 cut(s) 55, 253
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 3 cut(s) 97, 988, 1199
BfaI CTAG 2 cut(s) 65, 279
BfmI CTRYAG 3 cut(s) 836, 1027, 1089
BfoI RGCGCY 1 cut(s) 152
BisI GCNGC 8 cut(s) 72, 110, 113, 116, 169, 888, 944, 1030
BlpI GCTNAGC 1 cut(s) 1247
BlsI GCNGC 8 cut(s) 73, 111, 114, 117, 170, 889, 945, 1031
Bme1390I CCNGG 2 cut(s) 186, 914
Bme18I GGWCC 4 cut(s) 303, 370, 478, 558
BmeT110I CYCGRG 2 cut(s) 645, 956
BmgT120I GGNCC 5 cut(s) 303, 370, 463, 478, 558
BmiI GGNNCC 4 cut(s) 150, 305, 480, 969
BmrFI CCNGG 2 cut(s) 186, 914
BmsI GCATC 1 cut(s) 1153
BpiI GAAGAC 3 cut(s) 764, 906, 1156
BplI GAGNNNNNCTC 2 cut(s) 641, 673
Bpu10I CCTNAGC 1 cut(s) 262
Bpu1102I GCTNAGC 1 cut(s) 1247
BpuMI CCSGG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 3 cut(s) 32, 149, 877
BsaI GGTCTC 1 cut(s) 1199
BsaJI CCNNGG 3 cut(s) 366, 601, 1188
Bsc4I CCNNNNNNNGG 1 cut(s) 919
Bse1I ACTGG 1 cut(s) 613
Bse3DI GCAATG 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 1 cut(s) 914
BseDI CCNNGG 3 cut(s) 366, 601, 1188
BseGI GGATG 4 cut(s) 95, 415, 760, 1144
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 919
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 5 cut(s) 258, 689, 983, 1205, 1261
BseNI ACTGG 1 cut(s) 613
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 1 cut(s) 970
BseXI GCAGC 4 cut(s) 58, 874, 930, 1041
BseYI CCCAGC 2 cut(s) 234, 1237
BshFI GGCC 1 cut(s) 464
BshNI GGYRCC 2 cut(s) 148, 967
BsiHKAI GWGCWC 1 cut(s) 676
BsiHKCI CYCGRG 2 cut(s) 645, 956
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 2 cut(s) 289, 619
BslI CCNNNNNNNGG 1 cut(s) 919
BsmAI GTCTC 3 cut(s) 97, 988, 1199
BsmFI GGGAC 2 cut(s) 289, 619
BsnI GGCC 1 cut(s) 464
Bso31I GGTCTC 1 cut(s) 1199
BsoBI CYCGRG 2 cut(s) 645, 956
Bsp1286I GDGCHC 2 cut(s) 676, 970
Bsp143I GATC 4 cut(s) 55, 253, 786, 922
Bsp1720I GCTNAGC 1 cut(s) 1247
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 4 cut(s) 110, 113, 116, 169
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 5 cut(s) 257, 690, 984, 1206, 1260
BspLI GGNNCC 4 cut(s) 150, 305, 480, 969
BspMAI CTGCAG 2 cut(s) 1031, 1093
BspPI GGATC 1 cut(s) 930
BspQI GCTCTTC 1 cut(s) 1074
BspT107I GGYRCC 2 cut(s) 148, 967
BspTNI GGTCTC 1 cut(s) 1199
BsrDI GCAATG 1 cut(s) 178
BsrI ACTGG 1 cut(s) 613
BssECI CCNNGG 3 cut(s) 366, 601, 1188
BssMI GATC 4 cut(s) 55, 253, 786, 922
BssNI GRCGYC 3 cut(s) 32, 149, 877
BssT1I CCWWGG 2 cut(s) 366, 1188
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 5 cut(s) 137, 627, 797, 857, 999
Bst6I CTCTTC 2 cut(s) 438, 1074
BstACI GRCGYC 3 cut(s) 32, 149, 877
BstC8I GCNNGC 3 cut(s) 107, 833, 1275
BstDEI CTNAG 7 cut(s) 244, 262, 677, 698, 992, 1214, 1247
BstDSI CCRYGG 2 cut(s) 366, 601
BstENI CCTNNNNNAGG 1 cut(s) 917
BstF5I GGATG 4 cut(s) 95, 415, 760, 1144
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 3 cut(s) 128, 151, 919
BstKTI GATC 4 cut(s) 58, 256, 789, 925
BstMAI GTCTC 3 cut(s) 97, 988, 1199
BstMBI GATC 4 cut(s) 55, 253, 786, 922
BstMWI GCNNNNNNNGC 5 cut(s) 115, 240, 597, 1141, 1279
BstNI CCWGG 1 cut(s) 914
BstSCI CCNGG 2 cut(s) 184, 912
BstSFI CTRYAG 3 cut(s) 836, 1027, 1089
BstSLI GKGCMC 1 cut(s) 970
BstV1I GCAGC 4 cut(s) 58, 874, 930, 1041
BstV2I GAAGAC 3 cut(s) 764, 906, 1156
BstXI CCANNNNNNTGG 1 cut(s) 300
BsuRI GGCC 1 cut(s) 464
BtgI CCRYGG 2 cut(s) 366, 601
BtsCI GGATG 4 cut(s) 95, 415, 760, 1144
BtsI GCAGTG 1 cut(s) 848
BtsIMutI CAGTG 2 cut(s) 426, 848
Cac8I GCNNGC 3 cut(s) 107, 833, 1275
CaiI CAGNNNCTG 2 cut(s) 251, 547
CfoI GCGC 3 cut(s) 128, 151, 919
Cfr13I GGNCC 5 cut(s) 303, 370, 463, 478, 558
CseI GACGC 1 cut(s) 911
CviAII CATG 5 cut(s) 181, 257, 367, 458, 727
DdeI CTNAG 7 cut(s) 244, 262, 677, 698, 992, 1214, 1247
DinI GGCGCC 1 cut(s) 150
DpnI GATC 4 cut(s) 57, 255, 788, 924
DpnII GATC 4 cut(s) 55, 253, 786, 922
DrdI GACNNNNNNGTC 1 cut(s) 1177
DseDI GACNNNNNNGTC 1 cut(s) 1177
Eam1104I CTCTTC 2 cut(s) 438, 1074
EarI CTCTTC 2 cut(s) 438, 1074
Eco130I CCWWGG 2 cut(s) 366, 1188
Eco31I GGTCTC 1 cut(s) 1199
Eco47I GGWCC 4 cut(s) 303, 370, 478, 558
Eco57I CTGAAG 1 cut(s) 85
Eco88I CYCGRG 2 cut(s) 645, 956
EcoNI CCTNNNNNAGG 1 cut(s) 917
EcoRII CCWGG 1 cut(s) 912
EcoT14I CCWWGG 2 cut(s) 366, 1188
EcoT22I ATGCAT 1 cut(s) 1146
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 366, 1188
FaeI CATG 5 cut(s) 184, 260, 370, 461, 730
FaqI GGGAC 2 cut(s) 289, 619
FatI CATG 5 cut(s) 180, 256, 366, 457, 726
FbaI TGATCA 2 cut(s) 55, 253
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 8 cut(s) 72, 110, 113, 116, 169, 888, 944, 1030
FokI GGATG 4 cut(s) 82, 422, 767, 1131
Fsp4HI GCNGC 8 cut(s) 72, 110, 113, 116, 169, 888, 944, 1030
FspBI CTAG 2 cut(s) 65, 279
GlaI GCGC 3 cut(s) 127, 150, 918
GluI GCNGC 8 cut(s) 72, 110, 113, 116, 169, 888, 944, 1030
GsaI CCCAGC 2 cut(s) 238, 1241
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 1 cut(s) 464
HapII CCGG 1 cut(s) 185
HgaI GACGC 1 cut(s) 911
HhaI GCGC 3 cut(s) 128, 151, 919
Hin1I GRCGYC 3 cut(s) 32, 149, 877
Hin1II CATG 5 cut(s) 184, 260, 370, 461, 730
Hin6I GCGC 3 cut(s) 126, 149, 917
HinP1I GCGC 3 cut(s) 126, 149, 917
HincII GTYRAC 2 cut(s) 694, 719
HindII GTYRAC 2 cut(s) 694, 719
HindIII AAGCTT 1 cut(s) 506
HinfI GANTC 5 cut(s) 472, 544, 565, 1070, 1216
HpaII CCGG 1 cut(s) 185
HphI GGTGA 2 cut(s) 796, 1189
Hpy166II GTNNAC 6 cut(s) 284, 426, 454, 558, 694, 719
Hpy188III TCNNGA 4 cut(s) 224, 476, 1074, 1168
Hpy8I GTNNAC 6 cut(s) 284, 426, 454, 558, 694, 719
Hpy99I CGWCG 3 cut(s) 34, 37, 1302
HpyAV CCTTC 2 cut(s) 109, 602
HpyCH4III ACNGT 5 cut(s) 137, 627, 797, 857, 999
HpyCH4IV ACGT 3 cut(s) 32, 877, 1300
HpyCH4V TGCA 5 cut(s) 809, 946, 1029, 1091, 1144
HpyF10VI GCNNNNNNNGC 5 cut(s) 115, 240, 597, 1141, 1279
HpyF3I CTNAG 7 cut(s) 244, 262, 677, 698, 992, 1214, 1247
HpySE526I ACGT 3 cut(s) 32, 877, 1300
Hsp92I GRCGYC 3 cut(s) 32, 149, 877
Hsp92II CATG 5 cut(s) 184, 260, 370, 461, 730
HspAI GCGC 3 cut(s) 126, 149, 917
KasI GGCGCC 1 cut(s) 148
Ksp22I TGATCA 2 cut(s) 55, 253
Kzo9I GATC 4 cut(s) 55, 253, 786, 922
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 4 cut(s) 58, 874, 930, 1041
LweI GCATC 1 cut(s) 1153
MaeI CTAG 2 cut(s) 65, 279
MaeII ACGT 3 cut(s) 32, 877, 1300
MaeIII GTNAC 1 cut(s) 976
MalI GATC 4 cut(s) 57, 255, 788, 924
MboI GATC 4 cut(s) 55, 253, 786, 922
MboII GAAGA 9 cut(s) 239, 455, 643, 754, 764, 911, 980, 1091, 1156
MhlI GDGCHC 2 cut(s) 676, 970
MluCI AATT 5 cut(s) 804, 882, 1009, 1095, 1156
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 2 cut(s) 1079, 1225
MnlI CCTC 6 cut(s) 400, 439, 508, 520, 528, 957
Mph1103I ATGCAT 1 cut(s) 1146
MroXI GAANNNNTTC 2 cut(s) 422, 569
MseI TTAA 2 cut(s) 551, 908
MslI CAYNNNNRTG 2 cut(s) 431, 600
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 2 cut(s) 186, 914
MvaI CCWGG 1 cut(s) 914
MwoI GCNNNNNNNGC 5 cut(s) 115, 240, 597, 1141, 1279
NarI GGCGCC 1 cut(s) 149
NciI CCSGG 1 cut(s) 186
NcoI CCATGG 1 cut(s) 366
NdeII GATC 4 cut(s) 55, 253, 786, 922
NlaIII CATG 5 cut(s) 184, 260, 370, 461, 730
NlaIV GGNNCC 4 cut(s) 150, 305, 480, 969
NmuCI GTSAC 1 cut(s) 976
NsiI ATGCAT 1 cut(s) 1146
PciSI GCTCTTC 1 cut(s) 1074
PdmI GAANNNNTTC 2 cut(s) 422, 569
PfeI GAWTC 3 cut(s) 472, 544, 565
PkrI GCNGC 8 cut(s) 73, 111, 114, 117, 170, 889, 945, 1031
PleI GAGTC 2 cut(s) 1078, 1224
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 2 cut(s) 1078, 1224
PshBI ATTAAT 1 cut(s) 908
Psp6I CCWGG 1 cut(s) 912
PspFI CCCAGC 2 cut(s) 234, 1237
PspGI CCWGG 1 cut(s) 912
PspN4I GGNNCC 4 cut(s) 150, 305, 480, 969
PspPI GGNCC 5 cut(s) 303, 370, 463, 478, 558
PstI CTGCAG 2 cut(s) 1031, 1093
PstNI CAGNNNCTG 2 cut(s) 251, 547
RseI CAYNNNNRTG 2 cut(s) 431, 600
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 2 cut(s) 551, 908
SatI GCNGC 8 cut(s) 72, 110, 113, 116, 169, 888, 944, 1030
Sau3AI GATC 4 cut(s) 55, 253, 786, 922
Sau96I GGNCC 5 cut(s) 303, 370, 463, 478, 558
SchI GAGTC 2 cut(s) 1079, 1225
ScrFI CCNGG 2 cut(s) 186, 914
SduI GDGCHC 2 cut(s) 676, 970
SfaNI GCATC 1 cut(s) 1153
SfcI CTRYAG 3 cut(s) 836, 1027, 1089
SfoI GGCGCC 1 cut(s) 150
SinI GGWCC 4 cut(s) 303, 370, 478, 558
SmiMI CAYNNNNRTG 2 cut(s) 431, 600
Sse9I AATT 5 cut(s) 804, 882, 1009, 1095, 1156
SsiI CCGC 4 cut(s) 110, 113, 116, 169
SspDI GGCGCC 1 cut(s) 148
SspI AATATT 1 cut(s) 734
SspMI CTAG 2 cut(s) 65, 279
StyD4I CCNGG 2 cut(s) 184, 912
StyI CCWWGG 2 cut(s) 366, 1188
TaaI ACNGT 5 cut(s) 137, 627, 797, 857, 999
TaiI ACGT 3 cut(s) 35, 880, 1303
TaqI TCGA 1 cut(s) 789
TasI AATT 5 cut(s) 804, 882, 1009, 1095, 1156
TauI GCSGC 4 cut(s) 112, 115, 118, 171
TfiI GAWTC 3 cut(s) 472, 544, 565
Tru1I TTAA 2 cut(s) 551, 908
Tru9I TTAA 2 cut(s) 551, 908
TscAI CASTG 2 cut(s) 433, 848
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 4 cut(s) 71, 887, 943, 1029
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 4 cut(s) 189, 584, 719, 771
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 2 cut(s) 433, 848
VpaK11BI GGWCC 4 cut(s) 303, 370, 478, 558
VspI ATTAAT 1 cut(s) 908
XagI CCTNNNNNAGG 1 cut(s) 917
XmiI GTMKAC 1 cut(s) 453
XmnI GAANNNNTTC 2 cut(s) 422, 569
XspI CTAG 2 cut(s) 65, 279
ZraI GACGTC 2 cut(s) 33, 878
Zsp2I ATGCAT 1 cut(s) 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.