AT4G09750

dehydrogenase reductase SDR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013243
Physical Location & Seq
Forward (+)
6146623 .. 6148814
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G09750.1

Sequence Viewer

Length: 969 bp
ATGTTTCTCTTAAAGACATGGAGATCAACCGCTTTTGGGGTTTATGGCTACATGAATTTCACCAAGAGTGGTTTCTTAGATCATTCAAAGAAGTTTAAACCTGAAGATATGCAGTTACAAATTGAAGGAAAGAATTGTGTAGTTACTGGAGCTAATTCAGGTATTGGTTTTGCTGCTGCAGAGGGTCTTGCTTCACGTGGAGCAACTGTTTACATGGTGTGCCGGAACAAGGAAAGAGGACAAGAGGCTCTTTCTAAGATTCAAACCTCAACAGGAAACCAAAATGTGTACTTGGAAGTATGTGATTTGTCTTCCGTTAATGAAATCAAATCATTTGCTTCGAGTTTCGCTTCCAAGGATGTTCCGGTTCATGTGCTGGTTAATAATGCTGGTTTGCTTGAGAACAAACGTACTACTACACCTGAAGGATTCGAGTTAAGTTTTGCGGTGAATGTACTTGGGACATACACAATGACAGAGTTGATGCTTCCATTGTTGGAGAAAGCAACACCAGACGCCAAAGTCATTACAGTTGCCTCTGGTGGAATGTACACTTCGCCGCTTACTACAGATCTTCAGTTTAGTGGTGAAAAATTTGATGGAGTGGAACAATACGCACGGAACAAAAGAATCCAGGTGGCTCTGACAGAAAAATGGGCTGATAAGTACAAAAATAAAGGCATAGGGTTCTACTCAATGCACCCTGGCTGGGCTGAGACACCAGGTGTTGCCAAGAGTTTACCGAGTTTCAGTGAATCGTTCGCGGGTAAGCTTAGAACAAGCGAACAAGGAGCCGATACAATCGTTTGGTTAGCACTGCAGCCAAAAGAGAAGCTTGTCTCTGGTGCATTCTATTTCGATAGAGCTGAAGCACCAAAACATCTAAAGCTTGCAGGTACAAGCAAGTCTCATGACCTTATTGACTCAGTCATTGATTCTGTGCATTCCATGGCAGCTCTTAATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

322

Amino Acids

35.16

Weight (kDa)

8.25

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 44 - 245 1.7e-26 short chain dehydrogenase
KR PF08659 47 - 184 3.4e-07 KR domain
Epimerase PF01370 47 - 228 3.4e-07 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 50 - 271 3.4e-21 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09750
fragaria_vesca FvH4_2g12970
malus_domestica MD10G1036200.v1.1
prunus_persica Prupe.8G041900_v2.0.a1 Prupe.8G041900_v2.0.a1 Prupe.8G041900_v2.0.a1
pyrus_communis pycom10g02460
rosa_chinensis RchiOBHm_Chr6g0274101
rosa_laevigata RLG00000013578
rosa_multiflora Rmu_ssc0000413.1_g000003
rosa_roxburghii Rroxscaffold_7G00194650
rosa_rugosa Rorug06G0078500 Rorug06G0078500
rosa_samantha Rh6AG194500 Rh6BG198600 Rh6CG198400
rosa_wichuraiana Rw0G007930 Rw6G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 884
AccII CGCG 1 cut(s) 764
AciI CCGC 4 cut(s) 30, 446, 560, 764
AcsI RAATTY 2 cut(s) 55, 593
AcuI CTGAAG 4 cut(s) 123, 444, 560, 888
AcvI CACGTG 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 516
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 6 cut(s) 290, 412, 456, 551, 668, 898
AfiI CCNNNNNNNGG 3 cut(s) 36, 229, 709
AgsI TTSAA 3 cut(s) 87, 125, 263
AjnI CCWGG 3 cut(s) 633, 703, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 6 cut(s) 152, 772, 835, 866, 889, 956
AluI AGCT 6 cut(s) 152, 772, 835, 866, 889, 956
Alw26I GTCTC 3 cut(s) 710, 844, 912
ApeKI GCWGC 4 cut(s) 173, 176, 820, 953
ApoI RAATTY 2 cut(s) 55, 593
AsuHPI GGTGA 3 cut(s) 52, 460, 599
BaeI ACNNNNGTAYC 2 cut(s) 789, 822
BbrPI CACGTG 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 4 cut(s) 160, 163, 832, 965
BccI CCATC 1 cut(s) 593
BciT130I CCWGG 3 cut(s) 635, 705, 723
BcoDI GTCTC 3 cut(s) 710, 844, 912
BfmI CTRYAG 3 cut(s) 177, 567, 818
BfuAI ACCTGC 1 cut(s) 884
BglII AGATCT 1 cut(s) 571
BisI GCNGC 5 cut(s) 174, 177, 560, 821, 954
BlsI GCNGC 5 cut(s) 175, 178, 561, 822, 955
Bme1390I CCNGG 3 cut(s) 635, 705, 723
BmiI GGNNCC 1 cut(s) 793
BmrFI CCNGG 3 cut(s) 635, 705, 723
BmsI GCATC 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 419
BsaAI YACGTR 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 516
BsaJI CCNNGG 3 cut(s) 354, 703, 948
BsaWI WCCGGW 1 cut(s) 364
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 229, 709
Bse1I ACTGG 1 cut(s) 151
BseBI CCWGG 3 cut(s) 635, 705, 723
BseDI CCNNGG 3 cut(s) 354, 703, 948
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 3 cut(s) 36, 229, 709
BseMII CTCAG 2 cut(s) 705, 939
BseNI ACTGG 1 cut(s) 151
BseXI GCAGC 4 cut(s) 160, 163, 832, 965
BseYI CCCAGC 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 764
BsiSI CCGG 2 cut(s) 223, 365
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 3 cut(s) 36, 229, 709
BsmAI GTCTC 3 cut(s) 710, 844, 912
BsmFI GGGAC 1 cut(s) 475
BsmI GAATGC 2 cut(s) 848, 943
Bsp1407I TGTACA 1 cut(s) 549
Bsp143I GATC 3 cut(s) 23, 79, 571
Bsp19I CCATGG 1 cut(s) 948
BspACI CCGC 4 cut(s) 30, 446, 560, 764
BspCNI CTCAG 2 cut(s) 706, 938
BspFNI CGCG 1 cut(s) 764
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 1 cut(s) 793
BspMAI CTGCAG 2 cut(s) 181, 822
BspMI ACCTGC 1 cut(s) 884
BsrGI TGTACA 1 cut(s) 549
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 3 cut(s) 354, 703, 948
BssMI GATC 3 cut(s) 23, 79, 571
BssNI GRCGYC 1 cut(s) 516
BssT1I CCWWGG 2 cut(s) 354, 948
Bst2UI CCWGG 3 cut(s) 635, 705, 723
Bst4CI ACNGT 2 cut(s) 208, 532
BstACI GRCGYC 1 cut(s) 516
BstAUI TGTACA 1 cut(s) 549
BstBAI YACGTR 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 891
BstDEI CTNAG 5 cut(s) 76, 255, 714, 773, 925
BstDSI CCRYGG 1 cut(s) 948
BstF5I GGATG 1 cut(s) 364
BstFNI CGCG 1 cut(s) 764
BstKTI GATC 3 cut(s) 26, 82, 574
BstMAI GTCTC 3 cut(s) 710, 844, 912
BstMBI GATC 3 cut(s) 23, 79, 571
BstNI CCWGG 3 cut(s) 635, 705, 723
BstSCI CCNGG 3 cut(s) 633, 703, 721
BstSFI CTRYAG 3 cut(s) 177, 567, 818
BstUI CGCG 1 cut(s) 764
BstV1I GCAGC 4 cut(s) 160, 163, 832, 965
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 571
BstYI RGATCY 1 cut(s) 571
BtgI CCRYGG 1 cut(s) 948
BtsCI GGATG 1 cut(s) 364
BtsI GCAGTG 1 cut(s) 815
BtsIMutI CAGTG 2 cut(s) 757, 815
BveI ACCTGC 1 cut(s) 884
Cac8I GCNNGC 1 cut(s) 891
CciI TCATGA 1 cut(s) 910
CseI GACGC 1 cut(s) 524
CsiI ACCWGGT 1 cut(s) 721
Csp6I GTAC 6 cut(s) 289, 411, 455, 550, 667, 897
CviAII CATG 6 cut(s) 18, 52, 214, 371, 911, 949
CviQI GTAC 6 cut(s) 289, 411, 455, 550, 667, 897
DdeI CTNAG 5 cut(s) 76, 255, 714, 773, 925
DpnI GATC 3 cut(s) 25, 81, 573
DpnII GATC 3 cut(s) 23, 79, 571
DraI TTTAAA 1 cut(s) 97
DraIII CACNNNGTG 1 cut(s) 725
DrdI GACNNNNNNGTC 1 cut(s) 521
DseDI GACNNNNNNGTC 1 cut(s) 521
Eco130I CCWWGG 2 cut(s) 354, 948
Eco57I CTGAAG 4 cut(s) 123, 444, 560, 888
Eco72I CACGTG 1 cut(s) 197
EcoRII CCWGG 3 cut(s) 633, 703, 721
EcoT14I CCWWGG 2 cut(s) 354, 948
ErhI CCWWGG 2 cut(s) 354, 948
FaeI CATG 6 cut(s) 21, 55, 217, 374, 914, 952
FalI AAGNNNNNCTT 4 cut(s) 234, 266, 819, 851
FaqI GGGAC 1 cut(s) 475
FatI CATG 6 cut(s) 17, 51, 213, 370, 910, 948
FauI CCCGC 1 cut(s) 757
Fnu4HI GCNGC 5 cut(s) 174, 177, 560, 821, 954
FokI GGATG 1 cut(s) 371
Fsp4HI GCNGC 5 cut(s) 174, 177, 560, 821, 954
GluI GCNGC 5 cut(s) 174, 177, 560, 821, 954
GsaI CCCAGC 1 cut(s) 712
GsuI CTGGAG 1 cut(s) 168
HapII CCGG 2 cut(s) 223, 365
HgaI GACGC 1 cut(s) 524
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 6 cut(s) 21, 55, 217, 374, 914, 952
HindIII AAGCTT 3 cut(s) 770, 833, 887
HinfI GANTC 6 cut(s) 259, 429, 630, 755, 923, 935
HpaII CCGG 2 cut(s) 223, 365
HphI GGTGA 3 cut(s) 52, 460, 599
Hpy166II GTNNAC 4 cut(s) 211, 289, 552, 740
Hpy188I TCNGA 1 cut(s) 645
Hpy188III TCNNGA 1 cut(s) 911
Hpy8I GTNNAC 4 cut(s) 211, 289, 552, 740
HpyAV CCTTC 2 cut(s) 119, 419
HpyCH4III ACNGT 2 cut(s) 208, 532
HpyCH4IV ACGT 2 cut(s) 196, 409
HpyCH4V TGCA 7 cut(s) 112, 179, 700, 820, 848, 893, 943
HpyF3I CTNAG 5 cut(s) 76, 255, 714, 773, 925
HpySE526I ACGT 2 cut(s) 196, 409
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 6 cut(s) 21, 55, 217, 374, 914, 952
Kzo9I GATC 3 cut(s) 23, 79, 571
LmnI GCTCC 3 cut(s) 149, 200, 791
Lsp1109I GCAGC 4 cut(s) 160, 163, 832, 965
LweI GCATC 1 cut(s) 474
MabI ACCWGGT 1 cut(s) 721
MaeII ACGT 2 cut(s) 196, 409
MaeIII GTNAC 2 cut(s) 114, 142
MalI GATC 3 cut(s) 25, 81, 573
MboI GATC 3 cut(s) 23, 79, 571
MboII GAAGA 3 cut(s) 116, 303, 566
MflI RGATCY 1 cut(s) 571
MluCI AATT 5 cut(s) 55, 120, 133, 154, 593
MlyI GAGTC 1 cut(s) 917
MmeI TCCRAC 1 cut(s) 477
MnlI CCTC 5 cut(s) 175, 230, 238, 277, 547
MseI TTAA 7 cut(s) 11, 96, 318, 381, 437, 960, 967
MspI CCGG 2 cut(s) 223, 365
MspR9I CCNGG 3 cut(s) 635, 705, 723
MssI GTTTAAAC 1 cut(s) 97
Mva1269I GAATGC 2 cut(s) 848, 943
MvaI CCWGG 3 cut(s) 635, 705, 723
MvnI CGCG 1 cut(s) 764
NcoI CCATGG 1 cut(s) 948
NdeII GATC 3 cut(s) 23, 79, 571
NlaIII CATG 6 cut(s) 21, 55, 217, 374, 914, 952
NlaIV GGNNCC 1 cut(s) 793
PagI TCATGA 1 cut(s) 910
PctI GAATGC 2 cut(s) 848, 943
PfeI GAWTC 5 cut(s) 259, 429, 630, 755, 935
PflFI GACNNNGTC 1 cut(s) 926
PkrI GCNGC 5 cut(s) 175, 178, 561, 822, 955
PleI GAGTC 1 cut(s) 917
PmaCI CACGTG 1 cut(s) 197
PmeI GTTTAAAC 1 cut(s) 97
PmlI CACGTG 1 cut(s) 197
PpsI GAGTC 1 cut(s) 917
Ppu21I YACGTR 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 633, 703, 721
PspCI CACGTG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 708
PspGI CCWGG 3 cut(s) 633, 703, 721
PspN4I GGNNCC 1 cut(s) 793
PstI CTGCAG 2 cut(s) 181, 822
PsuI RGATCY 1 cut(s) 571
PsyI GACNNNGTC 1 cut(s) 926
RsaI GTAC 6 cut(s) 290, 412, 456, 551, 668, 898
RsaNI GTAC 6 cut(s) 289, 411, 455, 550, 667, 897
SaqAI TTAA 7 cut(s) 11, 96, 318, 381, 437, 960, 967
SatI GCNGC 5 cut(s) 174, 177, 560, 821, 954
Sau3AI GATC 3 cut(s) 23, 79, 571
SchI GAGTC 1 cut(s) 917
ScrFI CCNGG 3 cut(s) 635, 705, 723
SexAI ACCWGGT 1 cut(s) 721
SfaNI GCATC 1 cut(s) 474
SfcI CTRYAG 3 cut(s) 177, 567, 818
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 5 cut(s) 55, 120, 133, 154, 593
SsiI CCGC 4 cut(s) 30, 446, 560, 764
StyD4I CCNGG 3 cut(s) 633, 703, 721
StyI CCWWGG 2 cut(s) 354, 948
TaaI ACNGT 2 cut(s) 208, 532
TaiI ACGT 2 cut(s) 199, 412
TaqI TCGA 3 cut(s) 341, 432, 858
TasI AATT 5 cut(s) 55, 120, 133, 154, 593
TatI WGTACW 4 cut(s) 288, 454, 549, 666
TauI GCSGC 1 cut(s) 562
TfiI GAWTC 5 cut(s) 259, 429, 630, 755, 935
Tru1I TTAA 7 cut(s) 11, 96, 318, 381, 437, 960, 967
Tru9I TTAA 7 cut(s) 11, 96, 318, 381, 437, 960, 967
TscAI CASTG 2 cut(s) 757, 822
TseI GCWGC 4 cut(s) 173, 176, 820, 953
TspDTI ATGAA 3 cut(s) 68, 336, 359
TspGWI ACGGA 2 cut(s) 304, 634
TspRI CASTG 2 cut(s) 757, 822
Tth111I GACNNNGTC 1 cut(s) 926
XapI RAATTY 2 cut(s) 55, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.