MD10G1036200.v1.1

Dehydrogenase reductase SDR family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013243
Physical Location & Seq
Reverse (-)
4745650 .. 4755040
9391 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1036200.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGGATGGGTTGGAGAGGGAGACTCCAACGACTGTAAGTCTTAGACTTGAACTTCAGTTATCCATTGGAGCTCTTGCAATGTTTCTTCTCAAGGCATGGAGACAAACAGCTTTTGGAGTTTATGGGTACCTGAATTTCACCAAGTCTGGTTTCATAGAACATTCAAAGACATTCAAGTCCGAGGATACGCAAGCTCGGATAGAAGGGAAAAATTGCATTGTAACAGGAGCTAATTCAGGCATTGGTTATGCAACTGCCGAGGGACTTGCATCACGTGGCGCAACTGTCTATATGGTTTGTCGGAATAAGGAGAGGGGTGAAACTGCACAGTCTAAAATCCAAACTGACACAGGAAACCAAAATGTTCATTTGGAGGTCTGTGATCTATCATCTGTTAGTGATATCAAGTCATTTGCGTCCAAGTTTTCCACAAAGAACGTGCCAGTTCACGTATTGGTGAACAATGCTGGTTTGCTTGAGCATAATAGAGTTACCACATCAGAGGGGTTTGAGTTGAACTTTGCTGTGAATGTATTAGGCACCTATGCCATGACGGAATTGATGTTGCCATTGCTGGAGAAAGCTACACCTGATGCCCGGGTTATTACTGTTTCCTCTGGTGGAATGTACACTACACCATTGACCAAGGATTTACAGTTTAGTGATGGGAACTTTAATGGCGTGGAACAATATGGTCGAAACAAGAGAGTTCAGGTGGCGTTAACAGAGAAATGGGCTGATATAAACAAAAACAAGGGGATTGGATTCTACTCAATGCACCCAGGTTGGGCTGAGACACCGGGGGTTGCCAAGAGTTTGCCAAGTTTCTCTAAAGCGCTATCGGGAAAGCTTAGAACGAACGAGCAAGGTGCAGACACCGTAATTTGGTTGGCTTTACAGCCTAAAGAAAAACTTGTATCGGGAGCATTTTATTTCGATAGAGCAGAAGCACCTAAACACCTTTTGTTTGCAGCAACAAGCCGGTCTCATACTGAAATCGACTCCATTTATGACACTCTAAGTAGCTTATCTAGGACCTCCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

349

Amino Acids

38.03

Weight (kDa)

8.28

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 70 - 270 2.5e-25 short chain dehydrogenase
KR PF08659 73 - 210 5.2e-09 KR domain
Epimerase PF01370 73 - 249 9.6e-09 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 76 - 280 1.8e-19 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09750
fragaria_vesca FvH4_2g12970
malus_domestica MD10G1036200.v1.1
prunus_persica Prupe.8G041900_v2.0.a1 Prupe.8G041900_v2.0.a1 Prupe.8G041900_v2.0.a1
pyrus_communis pycom10g02460
rosa_chinensis RchiOBHm_Chr6g0274101
rosa_laevigata RLG00000013578
rosa_multiflora Rmu_ssc0000413.1_g000003
rosa_roxburghii Rroxscaffold_7G00194650
rosa_rugosa Rorug06G0078500 Rorug06G0078500
rosa_samantha Rh6AG194500 Rh6BG198600 Rh6CG198400
rosa_wichuraiana Rw0G007930 Rw6G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 175
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 2 cut(s) 126, 539
AciI CCGC 1 cut(s) 1041
AcsI RAATTY 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 38
AcvI CACGTG 1 cut(s) 275
AfaI GTAC 2 cut(s) 128, 629
AfeI AGCGCT 1 cut(s) 837
AfiI CCNNNNNNNGG 2 cut(s) 787, 885
AgsI TTSAA 4 cut(s) 50, 165, 175, 517
AhdI GACNNNNNGTC 1 cut(s) 36
AjnI CCWGG 1 cut(s) 781
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AluBI AGCT 7 cut(s) 71, 110, 194, 230, 584, 850, 1026
AluI AGCT 7 cut(s) 71, 110, 194, 230, 584, 850, 1026
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 4 cut(s) 14, 94, 788, 990
Ama87I CYCGRG 1 cut(s) 597
Aor51HI AGCGCT 1 cut(s) 837
ApeKI GCWGC 1 cut(s) 971
ApoI RAATTY 1 cut(s) 133
Asp718I GGTACC 1 cut(s) 126
AspLEI GCGC 2 cut(s) 281, 838
AspS9I GGNCC 1 cut(s) 1035
AsuC2I CCSGG 3 cut(s) 598, 599, 801
AsuHPI GGTGA 3 cut(s) 130, 329, 469
AvaI CYCGRG 1 cut(s) 597
AvaII GGWCC 1 cut(s) 1035
BanI GGYRCC 2 cut(s) 126, 539
BanII GRGCYC 1 cut(s) 73
BbrPI CACGTG 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 983
BccI CCATC 1 cut(s) 659
BcgI CGANNNNNNTGC 4 cut(s) 248, 282, 851, 885
BciT130I CCWGG 1 cut(s) 783
BciVI GTATCC 1 cut(s) 178
BcnI CCSGG 3 cut(s) 598, 599, 801
BcoDI GTCTC 4 cut(s) 14, 94, 788, 990
BfaI CTAG 1 cut(s) 1032
BfoI RGCGCY 1 cut(s) 839
BfuI GTATCC 1 cut(s) 178
BisI GCNGC 1 cut(s) 972
BlsI GCNGC 1 cut(s) 973
Bme1390I CCNGG 4 cut(s) 598, 599, 783, 801
Bme18I GGWCC 1 cut(s) 1035
BmeRI GACNNNNNGTC 1 cut(s) 36
BmeT110I CYCGRG 1 cut(s) 597
BmgT120I GGNCC 1 cut(s) 1035
BmiI GGNNCC 2 cut(s) 128, 541
BmrFI CCNGG 4 cut(s) 598, 599, 783, 801
BmsI GCATC 2 cut(s) 278, 583
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 596
BpuEI CTTGAG 2 cut(s) 74, 497
BpuMI CCSGG 3 cut(s) 598, 599, 801
BsaAI YACGTR 2 cut(s) 275, 451
BsaI GGTCTC 1 cut(s) 990
BsaJI CCNNGG 6 cut(s) 180, 258, 597, 645, 781, 800
Bsc4I CCNNNNNNNGG 2 cut(s) 787, 885
Bse118I RCCGGY 1 cut(s) 981
Bse1I ACTGG 1 cut(s) 443
Bse3DI GCAATG 2 cut(s) 84, 569
BseBI CCWGG 1 cut(s) 783
BseDI CCNNGG 6 cut(s) 180, 258, 597, 645, 781, 800
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 787, 885
BseMI GCAATG 2 cut(s) 84, 569
BseMII CTCAG 1 cut(s) 783
BseNI ACTGG 1 cut(s) 443
BseXI GCAGC 1 cut(s) 983
BsgI GTGCAG 2 cut(s) 309, 891
BshNI GGYRCC 2 cut(s) 126, 539
BsiHKAI GWGCWC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 597
BsiSI CCGG 3 cut(s) 598, 800, 982
BslFI GGGAC 1 cut(s) 276
BslI CCNNNNNNNGG 2 cut(s) 787, 885
BsmAI GTCTC 4 cut(s) 14, 94, 788, 990
BsmFI GGGAC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 990
BsoBI CYCGRG 1 cut(s) 597
Bsp1286I GDGCHC 1 cut(s) 73
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 1 cut(s) 382
BspACI CCGC 1 cut(s) 1041
BspCNI CTCAG 1 cut(s) 784
BspLI GGNNCC 2 cut(s) 128, 541
BspT107I GGYRCC 2 cut(s) 126, 539
BspTNI GGTCTC 1 cut(s) 990
BsrDI GCAATG 2 cut(s) 84, 569
BsrFI RCCGGY 1 cut(s) 981
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 1 cut(s) 443
BssAI RCCGGY 1 cut(s) 981
BssECI CCNNGG 6 cut(s) 180, 258, 597, 645, 781, 800
BssMI GATC 1 cut(s) 382
BssT1I CCWWGG 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 6 cut(s) 34, 286, 330, 610, 657, 880
BstAUI TGTACA 1 cut(s) 627
BstBAI YACGTR 2 cut(s) 275, 451
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 4 cut(s) 41, 792, 851, 1019
BstF5I GGATG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 839
BstHHI GCGC 2 cut(s) 281, 838
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 4 cut(s) 14, 94, 788, 990
BstMBI GATC 1 cut(s) 382
BstNI CCWGG 1 cut(s) 783
BstSCI CCNGG 4 cut(s) 596, 597, 781, 799
BstV1I GCAGC 1 cut(s) 983
BsuI GTATCC 1 cut(s) 178
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 1 cut(s) 192
CfoI GCGC 2 cut(s) 281, 838
Cfr10I RCCGGY 1 cut(s) 981
Cfr13I GGNCC 1 cut(s) 1035
Cfr9I CCCGGG 1 cut(s) 597
CseI GACGC 1 cut(s) 405
Csp6I GTAC 2 cut(s) 127, 628
CviAII CATG 2 cut(s) 96, 550
CviQI GTAC 2 cut(s) 127, 628
DdeI CTNAG 4 cut(s) 41, 792, 851, 1019
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
DrdI GACNNNNNNGTC 1 cut(s) 175
DriI GACNNNNNGTC 1 cut(s) 36
DseDI GACNNNNNNGTC 1 cut(s) 175
Eam1105I GACNNNNNGTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 645
Eco24I GRGCYC 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 990
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 1 cut(s) 1035
Eco47III AGCGCT 1 cut(s) 837
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 38
Eco72I CACGTG 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 1035
EcoRII CCWGG 1 cut(s) 781
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 645
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 645
FaeI CATG 2 cut(s) 99, 553
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FaqI GGGAC 1 cut(s) 276
FatI CATG 2 cut(s) 95, 549
Fnu4HI GCNGC 1 cut(s) 972
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 1 cut(s) 972
FspBI CTAG 1 cut(s) 1032
GlaI GCGC 2 cut(s) 280, 837
GluI GCNGC 1 cut(s) 972
GsuI CTGGAG 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 839
HapII CCGG 3 cut(s) 598, 800, 982
HgaI GACGC 1 cut(s) 405
HhaI GCGC 2 cut(s) 281, 838
Hin1II CATG 2 cut(s) 99, 553
Hin6I GCGC 2 cut(s) 279, 836
HinP1I GCGC 2 cut(s) 279, 836
HincII GTYRAC 1 cut(s) 723
HindII GTYRAC 1 cut(s) 723
HindIII AAGCTT 1 cut(s) 848
HinfI GANTC 3 cut(s) 22, 765, 1001
HpaI GTTAAC 1 cut(s) 723
HpaII CCGG 3 cut(s) 598, 800, 982
HphI GGTGA 3 cut(s) 130, 329, 469
Hpy166II GTNNAC 4 cut(s) 448, 460, 630, 723
Hpy188I TCNGA 4 cut(s) 181, 198, 303, 502
Hpy188III TCNNGA 2 cut(s) 843, 921
Hpy8I GTNNAC 4 cut(s) 448, 460, 630, 723
HpyAV CCTTC 1 cut(s) 197
HpyCH4III ACNGT 6 cut(s) 34, 286, 330, 610, 657, 880
HpyCH4IV ACGT 3 cut(s) 274, 438, 450
HpyCH4V TGCA 8 cut(s) 77, 216, 251, 269, 326, 778, 872, 971
HpyF3I CTNAG 4 cut(s) 41, 792, 851, 1019
HpySE526I ACGT 3 cut(s) 274, 438, 450
Hsp92II CATG 2 cut(s) 99, 553
HspAI GCGC 2 cut(s) 279, 836
KpnI GGTACC 1 cut(s) 130
KspAI GTTAAC 1 cut(s) 723
Kzo9I GATC 1 cut(s) 382
LmnI GCTCC 3 cut(s) 68, 227, 923
Lsp1109I GCAGC 1 cut(s) 983
LweI GCATC 2 cut(s) 278, 583
MaeI CTAG 1 cut(s) 1032
MaeII ACGT 3 cut(s) 274, 438, 450
MaeIII GTNAC 2 cut(s) 220, 490
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MboII GAAGA 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 5 cut(s) 133, 211, 232, 557, 882
MlyI GAGTC 2 cut(s) 16, 995
MmeI TCCRAC 2 cut(s) 50, 281
MnlI CCTC 7 cut(s) 9, 175, 253, 306, 367, 496, 625
MseI TTAA 2 cut(s) 675, 722
MspI CCGG 3 cut(s) 598, 800, 982
MspR9I CCNGG 4 cut(s) 598, 599, 783, 801
MvaI CCWGG 1 cut(s) 783
NciI CCSGG 3 cut(s) 598, 599, 801
NdeII GATC 1 cut(s) 382
NlaIII CATG 2 cut(s) 99, 553
NlaIV GGNNCC 2 cut(s) 128, 541
NmeAIII GCCGAG 1 cut(s) 283
PfeI GAWTC 1 cut(s) 765
PkrI GCNGC 1 cut(s) 973
PleI GAGTC 2 cut(s) 16, 995
PmaCI CACGTG 1 cut(s) 275
PmlI CACGTG 1 cut(s) 275
PpsI GAGTC 2 cut(s) 16, 995
Ppu21I YACGTR 2 cut(s) 275, 451
PpuMI RGGWCCY 1 cut(s) 1035
Psp124BI GAGCTC 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 1035
Psp6I CCWGG 1 cut(s) 781
PspCI CACGTG 1 cut(s) 275
PspGI CCWGG 1 cut(s) 781
PspN4I GGNNCC 2 cut(s) 128, 541
PspPI GGNCC 1 cut(s) 1035
PspPPI RGGWCCY 1 cut(s) 1035
RsaI GTAC 2 cut(s) 128, 629
RsaNI GTAC 2 cut(s) 127, 628
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 2 cut(s) 675, 722
SatI GCNGC 1 cut(s) 972
Sau3AI GATC 1 cut(s) 382
Sau96I GGNCC 1 cut(s) 1035
SchI GAGTC 2 cut(s) 16, 995
ScrFI CCNGG 4 cut(s) 598, 599, 783, 801
SduI GDGCHC 1 cut(s) 73
SfaNI GCATC 2 cut(s) 278, 583
SinI GGWCC 1 cut(s) 1035
SmaI CCCGGG 1 cut(s) 599
SmlI CTYRAG 2 cut(s) 89, 476
SmoI CTYRAG 2 cut(s) 89, 476
Sse9I AATT 5 cut(s) 133, 211, 232, 557, 882
SsiI CCGC 1 cut(s) 1041
SspMI CTAG 1 cut(s) 1032
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 4 cut(s) 596, 597, 781, 799
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 6 cut(s) 34, 286, 330, 610, 657, 880
TaiI ACGT 3 cut(s) 277, 441, 453
TaqI TCGA 3 cut(s) 697, 936, 999
TasI AATT 5 cut(s) 133, 211, 232, 557, 882
TatI WGTACW 1 cut(s) 627
TfiI GAWTC 1 cut(s) 765
Tru1I TTAA 2 cut(s) 675, 722
Tru9I TTAA 2 cut(s) 675, 722
TseI GCWGC 1 cut(s) 971
TspDTI ATGAA 2 cut(s) 142, 356
TspGWI ACGGA 1 cut(s) 569
TspMI CCCGGG 1 cut(s) 597
VpaK11BI GGWCC 1 cut(s) 1035
XapI RAATTY 1 cut(s) 133
XmaI CCCGGG 1 cut(s) 597
XspI CTAG 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.