AT4G12130

Glycine cleavage T-protein C-terminal barrel domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004121
Physical Location & Seq
Forward (+)
7263576 .. 7265879
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12130.1

Sequence Viewer

Length: 1182 bp
ATGTTTCGATTCAATTTCCGTCGTGAAATCTCCAAATATACCGGAATTTATCACCGGAAGATACACAGCGGTCTAGAAGATGCCGGTCCGATGGCGTCTCGCCTTAAATCACGTTCCGTTGTCCGGTTTAGCGGACCGGATACGGTTAAGTTTCTTCAAGGTTTGTTAACCAACGATGTACGGAGATTCGGCGAATCCTCCGGCGAGAAGAACTCGGCGGTTCCTACGCCGAACATGGCTTCCGTTACGAATCCGCCTATGTACGCAGCGTTGTTAACTCCTCAAGGAAGGTTTCTATACGATTTTTTCTTGTATAGCCCTTCGAGACCCGATGAAAAGCTTGATCGGACTGGTTCAGGACCCGGGTCGGATTCGGGTCGTGATGGGTCTGTTGAGTTGTTTGCAGATGTTGATGTTGATGTCCTCGATGAATTGCTTGAAACTCTCAAAAAATATCGCTTGAGGTCCAAAGTGGATATTGAAAACGTTGCAGAGGAGTTTTCGTGTTGGCAGCGTTACGGTAGGAATCTTACTGGATCATCATCTGTTGGTTGGGGTGGTGGTGTTGATCGTGCTGGTGAGTCTACAGCTAGTGGTAATAAGTATGGATGGCAATGGTATAAGGATCCGAGGTTGGAGTGTCTCGGTTACAGAAGTATCTTTCCGTCTGATGCAACTCCGCCTTTAGTTGAGGCAGATAAAGAAACAGATGAAAGCAATTATCTTTTATGGAGATTAGAGCATGGAGTTGCTGAAGGATCAGCTGAAATTCCTAAAGGTGAAGCAATTCCGCTTGAATATAATTTCGTTGGTCTTAATGCTATAAGCTTTGACAAAGGCTGCTATGTTGGTCAAGAGCTTATAGCTCGTACCCATCACCGAGGTGTCATCCGCAAACGCTTAATCCCATTACGGTTTATCGATAGCAATGGAAAAGAGTTAAACCAGAAAATTGCGGCTGGAGCTGAAGTAGTAGAATCAGGAACGGGCAAGAAAATGGGGACGGTTTCAACAGCTCTGGGTAGCCGGGGAATGGGTGTAATGAGGGTAGAGGAAGCCTTTAAACCATCAGCTGAATTAGCAGTAAAGGACTCAGAGGAGGTGAAAGTCGAGGCAATCAAACCAACATGGTGGCCAGCTGAATGGTTCCAACAGAATCAATCAGGAGTTGCTGCAGCGTAG

Protein Analysis

393

Amino Acids

43.53

Weight (kDa)

6.3

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-barrel_CAF17_C PF25455 298 - 379 1.7e-14 CAF17, C-terminal beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 584
AciI CCGC 8 cut(s) 69, 132, 218, 254, 680, 791, 892, 956
AclI AACGTT 1 cut(s) 486
AclWI GGATC 4 cut(s) 544, 620, 633, 766
AcoI YGGCCR 1 cut(s) 1133
AcsI RAATTY 2 cut(s) 45, 768
AcuI CTGAAG 2 cut(s) 774, 987
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 3 cut(s) 180, 263, 871
AfiI CCNNNNNNNGG 2 cut(s) 123, 1033
AgsI TTSAA 6 cut(s) 13, 158, 440, 482, 797, 1011
AleI CACNNNNGTG 1 cut(s) 882
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
Alw26I GTCTC 3 cut(s) 102, 319, 647
AlwI GGATC 4 cut(s) 544, 620, 633, 766
Ama87I CYCGRG 1 cut(s) 362
AoxI GGCC 1 cut(s) 1133
ApeKI GCWGC 5 cut(s) 266, 511, 840, 1172, 1175
ApoI RAATTY 2 cut(s) 45, 768
Asp700I GAANNNNTTC 1 cut(s) 786
AspS9I GGNCC 4 cut(s) 86, 134, 359, 465
AsuC2I CCSGG 3 cut(s) 363, 364, 1028
AsuHPI GGTGA 5 cut(s) 44, 590, 791, 869, 1114
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 4 cut(s) 86, 134, 359, 465
BaeI ACNNNNGTAYC 2 cut(s) 640, 673
BalI TGGCCA 1 cut(s) 1135
BamHI GGATCC 1 cut(s) 625
BbvI GCAGC 4 cut(s) 278, 523, 827, 1159
BccI CCATC 5 cut(s) 85, 377, 603, 882, 1075
BciVI GTATCC 1 cut(s) 133
BcnI CCSGG 3 cut(s) 363, 364, 1028
BcoDI GTCTC 3 cut(s) 102, 319, 647
BfaI CTAG 2 cut(s) 74, 591
BfmI CTRYAG 2 cut(s) 585, 1173
BfuI GTATCC 1 cut(s) 133
BisI GCNGC 6 cut(s) 267, 512, 841, 957, 1173, 1176
BlsI GCNGC 6 cut(s) 268, 513, 842, 958, 1174, 1177
Bme1390I CCNGG 3 cut(s) 363, 364, 1028
Bme18I GGWCC 4 cut(s) 86, 134, 359, 465
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 4 cut(s) 86, 134, 359, 465
BmiI GGNNCC 4 cut(s) 222, 361, 627, 1148
BmrFI CCNGG 3 cut(s) 363, 364, 1028
BmsI GCATC 2 cut(s) 70, 661
BoxI GACNNNNGTC 1 cut(s) 364
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpmI CTGGAG 1 cut(s) 981
BpuEI CTTGAG 2 cut(s) 267, 481
BpuMI CCSGG 3 cut(s) 363, 364, 1028
Bsa29I ATCGAT 1 cut(s) 921
BsaBI GATNNNNATC 1 cut(s) 541
BsaHI GRCGYC 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 4 cut(s) 362, 629, 880, 1027
BsaWI WCCGGW 4 cut(s) 41, 54, 123, 136
BsaXI ACNNNNNCTCC 4 cut(s) 488, 518, 954, 984
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 1033
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 355, 538
Bse3DI GCAATG 2 cut(s) 620, 934
Bse8I GATNNNNATC 1 cut(s) 541
BseCI ATCGAT 1 cut(s) 921
BseDI CCNNGG 4 cut(s) 362, 629, 880, 1027
BseGI GGATG 2 cut(s) 614, 888
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 2 cut(s) 123, 1033
BseMI GCAATG 2 cut(s) 620, 934
BseMII CTCAG 1 cut(s) 1107
BseNI ACTGG 2 cut(s) 355, 538
BseRI GAGGAG 3 cut(s) 270, 509, 1112
BseXI GCAGC 4 cut(s) 278, 523, 827, 1159
BshFI GGCC 1 cut(s) 1135
BshVI ATCGAT 1 cut(s) 921
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 8 cut(s) 42, 55, 84, 124, 137, 201, 363, 1027
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 2 cut(s) 123, 1033
BsmAI GTCTC 3 cut(s) 102, 319, 647
BsmBI CGTCTC 1 cut(s) 102
BsmFI GGGAC 1 cut(s) 1015
BsnI GGCC 1 cut(s) 1135
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 362
Bsp143I GATC 5 cut(s) 343, 536, 568, 625, 758
BspACI CCGC 8 cut(s) 69, 132, 218, 254, 680, 791, 892, 956
BspANI GGCC 1 cut(s) 1135
BspCNI CTCAG 1 cut(s) 1106
BspDI ATCGAT 1 cut(s) 921
BspLI GGNNCC 4 cut(s) 222, 361, 627, 1148
BspMAI CTGCAG 1 cut(s) 1177
BspPI GGATC 4 cut(s) 544, 620, 633, 766
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 620, 934
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 355, 538
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 4 cut(s) 362, 629, 880, 1027
BssMI GATC 5 cut(s) 343, 536, 568, 625, 758
BssNI GRCGYC 1 cut(s) 95
Bst4CI ACNGT 4 cut(s) 145, 521, 915, 1006
BstACI GRCGYC 1 cut(s) 95
BstC8I GCNNGC 1 cut(s) 1137
BstDEI CTNAG 1 cut(s) 1093
BstF5I GGATG 2 cut(s) 614, 888
BstKTI GATC 5 cut(s) 346, 539, 571, 628, 761
BstMAI GTCTC 3 cut(s) 102, 319, 647
BstMBI GATC 5 cut(s) 343, 536, 568, 625, 758
BstMWI GCNNNNNNNGC 2 cut(s) 962, 1079
BstPAI GACNNNNGTC 1 cut(s) 364
BstSCI CCNGG 3 cut(s) 361, 362, 1026
BstSFI CTRYAG 2 cut(s) 585, 1173
BstV1I GCAGC 4 cut(s) 278, 523, 827, 1159
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 2 cut(s) 1131, 1143
BstYI RGATCY 1 cut(s) 625
Bsu15I ATCGAT 1 cut(s) 921
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 1 cut(s) 1135
BsuTUI ATCGAT 1 cut(s) 921
BtsCI GGATG 2 cut(s) 614, 888
Cac8I GCNNGC 1 cut(s) 1137
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 4 cut(s) 86, 134, 359, 465
Cfr9I CCCGGG 1 cut(s) 362
ClaI ATCGAT 1 cut(s) 921
CpoI CGGWCCG 2 cut(s) 86, 134
CseI GACGC 1 cut(s) 84
Csp6I GTAC 3 cut(s) 179, 262, 870
CspI CGGWCCG 2 cut(s) 86, 134
CviAII CATG 3 cut(s) 235, 743, 1128
CviQI GTAC 3 cut(s) 179, 262, 870
DdeI CTNAG 1 cut(s) 1093
DpnI GATC 5 cut(s) 345, 538, 570, 627, 760
DpnII GATC 5 cut(s) 343, 536, 568, 625, 758
DraI TTTAAA 1 cut(s) 1063
EaeI YGGCCR 1 cut(s) 1133
EciI GGCGGA 2 cut(s) 243, 669
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 4 cut(s) 86, 134, 359, 465
Eco57I CTGAAG 2 cut(s) 774, 987
Eco88I CYCGRG 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 359
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 3 cut(s) 238, 746, 1131
FaqI GGGAC 1 cut(s) 1015
FatI CATG 3 cut(s) 234, 742, 1127
FblI GTMKAC 1 cut(s) 584
Fnu4HI GCNGC 6 cut(s) 267, 512, 841, 957, 1173, 1176
FokI GGATG 2 cut(s) 621, 875
Fsp4HI GCNGC 6 cut(s) 267, 512, 841, 957, 1173, 1176
FspBI CTAG 2 cut(s) 74, 591
GluI GCNGC 6 cut(s) 267, 512, 841, 957, 1173, 1176
GsuI CTGGAG 1 cut(s) 981
HaeIII GGCC 1 cut(s) 1135
HapII CCGG 8 cut(s) 42, 55, 84, 124, 137, 201, 363, 1027
HgaI GACGC 1 cut(s) 84
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 3 cut(s) 238, 746, 1131
HincII GTYRAC 2 cut(s) 168, 276
HindII GTYRAC 2 cut(s) 168, 276
HindIII AAGCTT 2 cut(s) 338, 826
HpaI GTTAAC 2 cut(s) 168, 276
HpaII CCGG 8 cut(s) 42, 55, 84, 124, 137, 201, 363, 1027
HphI GGTGA 5 cut(s) 44, 590, 791, 869, 1114
Hpy166II GTNNAC 3 cut(s) 168, 276, 585
Hpy188I TCNGA 6 cut(s) 90, 348, 370, 630, 670, 1096
Hpy188III TCNNGA 8 cut(s) 23, 74, 324, 357, 380, 854, 981, 1164
Hpy8I GTNNAC 3 cut(s) 168, 276, 585
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 3 cut(s) 282, 330, 749
HpyCH4III ACNGT 4 cut(s) 145, 521, 915, 1006
HpyCH4IV ACGT 2 cut(s) 112, 486
HpyCH4V TGCA 4 cut(s) 404, 491, 674, 1175
HpyF10VI GCNNNNNNNGC 2 cut(s) 962, 1079
HpyF3I CTNAG 1 cut(s) 1093
HpySE526I ACGT 2 cut(s) 112, 486
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 3 cut(s) 238, 746, 1131
KspAI GTTAAC 2 cut(s) 168, 276
Kzo9I GATC 5 cut(s) 343, 536, 568, 625, 758
LmnI GCTCC 1 cut(s) 962
Lsp1109I GCAGC 4 cut(s) 278, 523, 827, 1159
LweI GCATC 2 cut(s) 70, 661
MaeI CTAG 2 cut(s) 74, 591
MaeII ACGT 2 cut(s) 112, 486
MaeIII GTNAC 3 cut(s) 244, 515, 647
MalI GATC 5 cut(s) 345, 538, 570, 627, 760
MboI GATC 5 cut(s) 343, 536, 568, 625, 758
MboII GAAGA 4 cut(s) 70, 89, 146, 220
MflI RGATCY 1 cut(s) 625
MlsI TGGCCA 1 cut(s) 1135
MluCI AATT 9 cut(s) 13, 45, 431, 718, 768, 786, 802, 951, 1076
MluNI TGGCCA 1 cut(s) 1135
MlyI GAGTC 2 cut(s) 590, 1085
MmeI TCCRAC 3 cut(s) 348, 615, 1174
Mox20I TGGCCA 1 cut(s) 1135
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 1135
MseI TTAA 8 cut(s) 105, 147, 167, 275, 816, 902, 941, 1062
MslI CAYNNNNRTG 1 cut(s) 882
Msp20I TGGCCA 1 cut(s) 1135
MspA1I CMGCKG 4 cut(s) 69, 764, 1073, 1139
MspI CCGG 8 cut(s) 42, 55, 84, 124, 137, 201, 363, 1027
MspR9I CCNGG 3 cut(s) 363, 364, 1028
MwoI GCNNNNNNNGC 2 cut(s) 962, 1079
NciI CCSGG 3 cut(s) 363, 364, 1028
NdeII GATC 5 cut(s) 343, 536, 568, 625, 758
NlaIII CATG 3 cut(s) 238, 746, 1131
NlaIV GGNNCC 4 cut(s) 222, 361, 627, 1148
NmeAIII GCCGAG 1 cut(s) 194
OliI CACNNNNGTG 1 cut(s) 882
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 8 cut(s) 9, 186, 194, 250, 371, 526, 977, 1156
PkrI GCNGC 6 cut(s) 268, 513, 842, 958, 1174, 1177
PleI GAGTC 2 cut(s) 589, 1085
PpsI GAGTC 2 cut(s) 589, 1085
PpuMI RGGWCCY 1 cut(s) 359
PshAI GACNNNNGTC 1 cut(s) 364
Psp1406I AACGTT 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 359
PspN4I GGNNCC 4 cut(s) 222, 361, 627, 1148
PspPI GGNCC 4 cut(s) 86, 134, 359, 465
PspPPI RGGWCCY 1 cut(s) 359
PstI CTGCAG 1 cut(s) 1177
PsuI RGATCY 1 cut(s) 625
PvuII CAGCTG 3 cut(s) 764, 1073, 1139
RsaI GTAC 3 cut(s) 180, 263, 871
RsaNI GTAC 3 cut(s) 179, 262, 870
RseI CAYNNNNRTG 1 cut(s) 882
Rsr2I CGGWCCG 2 cut(s) 86, 134
RsrII CGGWCCG 2 cut(s) 86, 134
SaqAI TTAA 8 cut(s) 105, 147, 167, 275, 816, 902, 941, 1062
SatI GCNGC 6 cut(s) 267, 512, 841, 957, 1173, 1176
Sau3AI GATC 5 cut(s) 343, 536, 568, 625, 758
Sau96I GGNCC 4 cut(s) 86, 134, 359, 465
SchI GAGTC 2 cut(s) 590, 1085
ScrFI CCNGG 3 cut(s) 363, 364, 1028
SfaNI GCATC 2 cut(s) 70, 661
SfcI CTRYAG 2 cut(s) 585, 1173
SinI GGWCC 4 cut(s) 86, 134, 359, 465
SmaI CCCGGG 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 882
SmlI CTYRAG 2 cut(s) 282, 460
SmoI CTYRAG 2 cut(s) 282, 460
Sse9I AATT 9 cut(s) 13, 45, 431, 718, 768, 786, 802, 951, 1076
SsiI CCGC 8 cut(s) 69, 132, 218, 254, 680, 791, 892, 956
SspMI CTAG 2 cut(s) 74, 591
StyD4I CCNGG 3 cut(s) 361, 362, 1026
TaaI ACNGT 4 cut(s) 145, 521, 915, 1006
TaiI ACGT 2 cut(s) 115, 489
TaqI TCGA 5 cut(s) 7, 323, 426, 921, 1110
TasI AATT 9 cut(s) 13, 45, 431, 718, 768, 786, 802, 951, 1076
TauI GCSGC 1 cut(s) 959
TfiI GAWTC 8 cut(s) 9, 186, 194, 250, 371, 526, 977, 1156
Tru1I TTAA 8 cut(s) 105, 147, 167, 275, 816, 902, 941, 1062
Tru9I TTAA 8 cut(s) 105, 147, 167, 275, 816, 902, 941, 1062
TseI GCWGC 5 cut(s) 266, 511, 840, 1172, 1175
TspDTI ATGAA 3 cut(s) 348, 444, 726
TspGWI ACGGA 5 cut(s) 8, 106, 196, 232, 654
TspMI CCCGGG 1 cut(s) 362
VpaK11BI GGWCC 4 cut(s) 86, 134, 359, 465
XapI RAATTY 2 cut(s) 45, 768
XbaI TCTAGA 1 cut(s) 73
XmaI CCCGGG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 2 cut(s) 74, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.