pycom04g07160

transferase CAF17 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004121
Physical Location & Seq
Forward (+)
7526785 .. 7528836
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g07160.1

Sequence Viewer

Length: 1191 bp
ATGCACCGTCTCAAACTCCCCCTGCAGTTCCCCAAATCCATTTCCTGCAGTTACAGAGCCCTCCACGACAAAACCCAGTTGGACAGCGCTGTGTCGCTGGCCTCCGTCCTCAAGTCGAGGGCGGTGGTCCGGTTCCGGGGTCCGGACACGGTCAAATTCCTTCAGGGTCTGCTGACCAACGACGTACGGAAGTTCGGAGAACCCATGGATGAGAAGACCTCCACTTTGGCCCCCCCCAATTTGTCCTGCGTGTCCGAACGGCCCATGTACGCCGCGCTGCTGACGCCCCAAGGGAGGTTCTTGTACGATTTCTTCTTGTACACTCCGCCCAGGCCCGATACCAAGCTCGATAGGACCGGGTCGGCGCCCGGCCCAGACCCAGATGAGGGGGTGGAGGTGTTCGCCGACGTGGATGTTTCCGTCTTGGATGAGCTATTGAGTACCTTGAAGAAATACCGTTTGAGGGCTAAGGTTGATATCGAGAATATGGCACAAGAGTTACAATGCTGGCAGCGTTATGCCGGAAACGTCTCTGAAAAATCCTCCTCTGTGGAAGAACCAGAGGCTGCTGGTGTTGATCGTTCAGGCATGTCATCTGCACGCGGGAATGCTCTTGGATGGCAATGGTTTAAGGATCCCAGATTGGATTGCCTTGGTTTCAGAGGGATCTTCCCGTCAGATACAATACCACCTTTGGTTGAAGCTGGCAAAGAAACAGATGAACAGAATTACCGTCTATGGAGAATAGAAAATGGAGTTGCCGAAGGCTCAACAGAGATCCCAAAAGGTGAGGCAATTCCTCTTGAGTATAACCTGGTGGGTCTAAATGCAATTAGCTTTGACAAGGGGTGTTATGTGGGCCAAGAGCTTATAGCTCGTACACACCATCGAGGGGTCATTCGCAAACGCTTGCTTCCGTTAAGATTTCTCGAGGAGAGTGGGGAAGAAGCAAAGCAGAACGTTGCTCCTGGCTCCGAAGTGATTGTTAGTGCCACGGGAAAGAAAGTCGGTACTGTCACAACTCAACTCGGGTGTCGTGGGCTGGGTGTCTTGCGTTTGGACGAAGCCTTCAAGAGATCAGGTTCATTGACCATACAAGGACAGAAAGATACAAAGGTTGAAGCTATTAAACCAGATTGGTGGCCTGCTGAATGGCTTCAAGAGCATCAACATCAAAGTGCAGTTGCTTAG

Protein Analysis

397

Amino Acids

43.98

Weight (kDa)

6.37

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-barrel_CAF17_C PF25455 301 - 382 7.9e-19 CAF17, C-terminal beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 364
AccII CGCG 2 cut(s) 275, 603
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 4 cut(s) 122, 273, 326, 603
AclI AACGTT 1 cut(s) 960
AclWI GGATC 4 cut(s) 629, 642, 674, 772
AcsI RAATTY 1 cut(s) 155
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 2 cut(s) 284, 365
AfaI GTAC 7 cut(s) 186, 269, 305, 320, 442, 880, 1012
AfeI AGCGCT 1 cut(s) 88
AfiI CCNNNNNNNGG 7 cut(s) 136, 142, 294, 385, 386, 463, 892
AgsI TTSAA 5 cut(s) 448, 701, 1072, 1121, 1160
AjiI CACGTC 1 cut(s) 409
AjnI CCWGG 3 cut(s) 329, 813, 967
AluBI AGCT 7 cut(s) 346, 433, 704, 837, 868, 875, 1124
AluI AGCT 7 cut(s) 346, 433, 704, 837, 868, 875, 1124
Alw26I GTCTC 2 cut(s) 14, 535
AlwI GGATC 4 cut(s) 629, 642, 674, 772
AlwNI CAGNNNCTG 2 cut(s) 169, 566
Ama87I CYCGRG 2 cut(s) 929, 1028
Aor13HI TCCGGA 1 cut(s) 142
Aor51HI AGCGCT 1 cut(s) 88
AoxI GGCC 7 cut(s) 99, 228, 260, 332, 370, 859, 1142
ApeKI GCWGC 3 cut(s) 277, 511, 566
ApoI RAATTY 1 cut(s) 155
ArsI GACNNNNNNTTYG 2 cut(s) 208, 240
Asp700I GAANNNNTTC 1 cut(s) 1155
AspLEI GCGC 3 cut(s) 89, 277, 367
AspS9I GGNCC 8 cut(s) 127, 140, 229, 261, 333, 354, 371, 859
AsuC2I CCSGG 3 cut(s) 137, 358, 369
AsuHPI GGTGA 1 cut(s) 800
AvaI CYCGRG 2 cut(s) 929, 1028
AvaII GGWCC 3 cut(s) 127, 140, 354
BaeI ACNNNNGTAYC 2 cut(s) 1101, 1134
BamHI GGATCC 1 cut(s) 634
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbsI GAAGAC 1 cut(s) 221
BbvI GCAGC 3 cut(s) 264, 523, 553
BccI CCATC 2 cut(s) 612, 894
BceAI ACGGC 1 cut(s) 275
BciT130I CCWGG 3 cut(s) 331, 815, 969
BcnI CCSGG 3 cut(s) 137, 358, 369
BcoDI GTCTC 2 cut(s) 14, 535
BfmI CTRYAG 2 cut(s) 23, 46
BfoI RGCGCY 2 cut(s) 90, 368
BisI GCNGC 4 cut(s) 273, 278, 512, 567
BlsI GCNGC 4 cut(s) 274, 279, 513, 568
Bme1390I CCNGG 6 cut(s) 137, 331, 358, 369, 815, 969
Bme18I GGWCC 3 cut(s) 127, 140, 354
BmeT110I CYCGRG 2 cut(s) 929, 1028
BmgBI CACGTC 1 cut(s) 409
BmgT120I GGNCC 8 cut(s) 127, 140, 229, 261, 333, 354, 371, 859
BmiI GGNNCC 6 cut(s) 134, 141, 231, 366, 636, 973
BmrFI CCNGG 6 cut(s) 137, 331, 358, 369, 815, 969
BmrI ACTGGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 1174
BmuI ACTGGG 1 cut(s) 70
BpiI GAAGAC 1 cut(s) 221
BplI GAGNNNNNCTC 2 cut(s) 203, 235
Bpu10I CCTNAGC 1 cut(s) 468
BpuEI CTTGAG 2 cut(s) 95, 824
BpuMI CCSGG 3 cut(s) 137, 358, 369
BsaHI GRCGYC 2 cut(s) 284, 365
BsaJI CCNNGG 6 cut(s) 136, 204, 289, 329, 652, 993
BsaWI WCCGGW 2 cut(s) 129, 142
Bsc4I CCNNNNNNNGG 7 cut(s) 136, 142, 294, 385, 386, 463, 892
Bse1I ACTGG 1 cut(s) 76
Bse3DI GCAATG 1 cut(s) 629
BseAI TCCGGA 1 cut(s) 142
BseBI CCWGG 3 cut(s) 331, 815, 969
BseDI CCNNGG 6 cut(s) 136, 204, 289, 329, 652, 993
BseGI GGATG 4 cut(s) 214, 418, 433, 623
BseLI CCNNNNNNNGG 7 cut(s) 136, 142, 294, 385, 386, 463, 892
BseMI GCAATG 1 cut(s) 629
BseNI ACTGG 1 cut(s) 76
BseRI GAGGAG 2 cut(s) 535, 947
BseXI GCAGC 3 cut(s) 264, 523, 553
BseYI CCCAGC 1 cut(s) 1042
BsgI GTGCAG 1 cut(s) 582
Bsh1236I CGCG 2 cut(s) 275, 603
BshFI GGCC 7 cut(s) 101, 230, 262, 334, 372, 861, 1144
BshNI GGYRCC 1 cut(s) 364
BsiHKCI CYCGRG 2 cut(s) 929, 1028
BsiSI CCGG 6 cut(s) 130, 136, 143, 357, 369, 522
BsiWI CGTACG 1 cut(s) 184
BslI CCNNNNNNNGG 7 cut(s) 136, 142, 294, 385, 386, 463, 892
BsmAI GTCTC 2 cut(s) 14, 535
BsmBI CGTCTC 2 cut(s) 14, 535
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 7 cut(s) 101, 230, 262, 334, 372, 861, 1144
BsoBI CYCGRG 2 cut(s) 929, 1028
Bsp1286I GDGCHC 1 cut(s) 61
Bsp13I TCCGGA 1 cut(s) 142
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 5 cut(s) 577, 634, 666, 777, 1076
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 4 cut(s) 122, 273, 326, 603
BspANI GGCC 7 cut(s) 101, 230, 262, 334, 372, 861, 1144
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 2 cut(s) 275, 603
BspLI GGNNCC 6 cut(s) 134, 141, 231, 366, 636, 973
BspMAI CTGCAG 2 cut(s) 27, 50
BspPI GGATC 4 cut(s) 629, 642, 674, 772
BspT107I GGYRCC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 629
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 6 cut(s) 136, 204, 289, 329, 652, 993
BssMI GATC 5 cut(s) 577, 634, 666, 777, 1076
BssNI GRCGYC 2 cut(s) 284, 365
BssT1I CCWWGG 3 cut(s) 204, 289, 652
Bst2UI CCWGG 3 cut(s) 331, 815, 969
Bst4CI ACNGT 5 cut(s) 8, 151, 458, 734, 1015
BstACI GRCGYC 2 cut(s) 284, 365
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 6 cut(s) 99, 509, 601, 706, 911, 1146
BstDEI CTNAG 2 cut(s) 468, 1188
BstDSI CCRYGG 2 cut(s) 204, 993
BstF5I GGATG 4 cut(s) 214, 418, 433, 623
BstFNI CGCG 2 cut(s) 275, 603
BstH2I RGCGCY 2 cut(s) 90, 368
BstHHI GCGC 3 cut(s) 89, 277, 367
BstKTI GATC 5 cut(s) 580, 637, 669, 780, 1079
BstMAI GTCTC 2 cut(s) 14, 535
BstMBI GATC 5 cut(s) 577, 634, 666, 777, 1076
BstMWI GCNNNNNNNGC 2 cut(s) 283, 1162
BstNI CCWGG 3 cut(s) 331, 815, 969
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 6 cut(s) 135, 329, 356, 367, 813, 967
BstSFI CTRYAG 2 cut(s) 23, 46
BstUI CGCG 2 cut(s) 275, 603
BstV1I GCAGC 3 cut(s) 264, 523, 553
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 3 cut(s) 634, 666, 777
BstXI CCANNNNNNTGG 1 cut(s) 1140
BstYI RGATCY 3 cut(s) 634, 666, 777
BsuRI GGCC 7 cut(s) 101, 230, 262, 334, 372, 861, 1144
BtgI CCRYGG 2 cut(s) 204, 993
BtrI CACGTC 1 cut(s) 409
BtsCI GGATG 4 cut(s) 214, 418, 433, 623
Cac8I GCNNGC 6 cut(s) 99, 509, 601, 706, 911, 1146
CaiI CAGNNNCTG 2 cut(s) 169, 566
CfoI GCGC 3 cut(s) 89, 277, 367
Cfr13I GGNCC 8 cut(s) 127, 140, 229, 261, 333, 354, 371, 859
CseI GACGC 1 cut(s) 292
CsiI ACCWGGT 1 cut(s) 813
Csp6I GTAC 7 cut(s) 185, 268, 304, 319, 441, 879, 1011
CviAII CATG 3 cut(s) 205, 265, 589
CviQI GTAC 7 cut(s) 185, 268, 304, 319, 441, 879, 1011
DdeI CTNAG 2 cut(s) 468, 1188
DinI GGCGCC 1 cut(s) 366
DpnI GATC 5 cut(s) 579, 636, 668, 779, 1078
DpnII GATC 5 cut(s) 577, 634, 666, 777, 1076
EciI GGCGGA 1 cut(s) 315
Eco130I CCWWGG 3 cut(s) 204, 289, 652
Eco24I GRGCYC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 3 cut(s) 127, 140, 354
Eco47III AGCGCT 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 146
Eco88I CYCGRG 2 cut(s) 929, 1028
EcoRII CCWGG 3 cut(s) 329, 813, 967
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 3 cut(s) 204, 289, 652
EcoT38I GRGCYC 1 cut(s) 61
EgeI GGCGCC 1 cut(s) 366
EheI GGCGCC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 204, 289, 652
Esp3I CGTCTC 2 cut(s) 14, 535
FaeI CATG 3 cut(s) 208, 268, 592
FatI CATG 3 cut(s) 204, 264, 588
FauI CCCGC 1 cut(s) 596
Fnu4HI GCNGC 4 cut(s) 273, 278, 512, 567
FokI GGATG 4 cut(s) 221, 425, 440, 630
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 4 cut(s) 273, 278, 512, 567
GlaI GCGC 3 cut(s) 88, 276, 366
GluI GCNGC 4 cut(s) 273, 278, 512, 567
GsaI CCCAGC 1 cut(s) 1046
HaeII RGCGCY 2 cut(s) 90, 368
HaeIII GGCC 7 cut(s) 101, 230, 262, 334, 372, 861, 1144
HapII CCGG 6 cut(s) 130, 136, 143, 357, 369, 522
HgaI GACGC 1 cut(s) 292
HhaI GCGC 3 cut(s) 89, 277, 367
Hin1I GRCGYC 2 cut(s) 284, 365
Hin1II CATG 3 cut(s) 208, 268, 592
Hin6I GCGC 3 cut(s) 87, 275, 365
HinP1I GCGC 3 cut(s) 87, 275, 365
HpaII CCGG 6 cut(s) 130, 136, 143, 357, 369, 522
HphI GGTGA 1 cut(s) 800
Hpy166II GTNNAC 2 cut(s) 321, 881
Hpy188I TCNGA 6 cut(s) 197, 256, 535, 662, 679, 976
Hpy188III TCNNGA 6 cut(s) 143, 481, 803, 929, 1072, 1160
Hpy8I GTNNAC 2 cut(s) 321, 881
Hpy99I CGWCG 2 cut(s) 185, 410
HpyAV CCTTC 3 cut(s) 170, 758, 1078
HpyCH4III ACNGT 5 cut(s) 8, 151, 458, 734, 1015
HpyCH4IV ACGT 4 cut(s) 183, 408, 528, 960
HpyCH4V TGCA 6 cut(s) 4, 25, 48, 599, 830, 1181
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 1162
HpyF3I CTNAG 2 cut(s) 468, 1188
HpySE526I ACGT 4 cut(s) 183, 408, 528, 960
Hsp92I GRCGYC 2 cut(s) 284, 365
Hsp92II CATG 3 cut(s) 208, 268, 592
HspAI GCGC 3 cut(s) 87, 275, 365
KasI GGCGCC 1 cut(s) 364
Kpn2I TCCGGA 1 cut(s) 142
Kzo9I GATC 5 cut(s) 577, 634, 666, 777, 1076
LmnI GCTCC 2 cut(s) 970, 977
Lsp1109I GCAGC 3 cut(s) 264, 523, 553
LweI GCATC 1 cut(s) 1174
MabI ACCWGGT 1 cut(s) 813
MaeII ACGT 4 cut(s) 183, 408, 528, 960
MaeIII GTNAC 3 cut(s) 50, 498, 1015
MalI GATC 5 cut(s) 579, 636, 668, 779, 1078
MboI GATC 5 cut(s) 577, 634, 666, 777, 1076
MboII GAAGA 6 cut(s) 226, 304, 460, 566, 661, 956
MflI RGATCY 3 cut(s) 634, 666, 777
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 5 cut(s) 155, 238, 727, 795, 831
Mly113I GGCGCC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 60
MroI TCCGGA 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 1155
MseI TTAA 3 cut(s) 630, 920, 1128
MslI CAYNNNNRTG 1 cut(s) 1176
MspI CCGG 6 cut(s) 130, 136, 143, 357, 369, 522
MspR9I CCNGG 6 cut(s) 137, 331, 358, 369, 815, 969
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 3 cut(s) 331, 815, 969
MvnI CGCG 2 cut(s) 275, 603
MwoI GCNNNNNNNGC 2 cut(s) 283, 1162
NarI GGCGCC 1 cut(s) 365
NciI CCSGG 3 cut(s) 137, 358, 369
NcoI CCATGG 1 cut(s) 204
NdeII GATC 5 cut(s) 577, 634, 666, 777, 1076
NlaIII CATG 3 cut(s) 208, 268, 592
NlaIV GGNNCC 6 cut(s) 134, 141, 231, 366, 636, 973
NmuCI GTSAC 1 cut(s) 1015
NspI RCATGY 1 cut(s) 592
PaeR7I CTCGAG 1 cut(s) 929
PctI GAATGC 1 cut(s) 613
PdmI GAANNNNTTC 1 cut(s) 1155
Pfl23II CGTACG 1 cut(s) 184
PflFI GACNNNGTC 2 cut(s) 149, 358
PkrI GCNGC 4 cut(s) 274, 279, 513, 568
PluTI GGCGCC 1 cut(s) 368
Psp1406I AACGTT 1 cut(s) 960
Psp6I CCWGG 3 cut(s) 329, 813, 967
PspFI CCCAGC 1 cut(s) 1042
PspGI CCWGG 3 cut(s) 329, 813, 967
PspLI CGTACG 1 cut(s) 184
PspN4I GGNNCC 6 cut(s) 134, 141, 231, 366, 636, 973
PspPI GGNCC 8 cut(s) 127, 140, 229, 261, 333, 354, 371, 859
PstI CTGCAG 2 cut(s) 27, 50
PstNI CAGNNNCTG 2 cut(s) 169, 566
PsuI RGATCY 3 cut(s) 634, 666, 777
PsyI GACNNNGTC 2 cut(s) 149, 358
RsaI GTAC 7 cut(s) 186, 269, 305, 320, 442, 880, 1012
RsaNI GTAC 7 cut(s) 185, 268, 304, 319, 441, 879, 1011
RseI CAYNNNNRTG 1 cut(s) 1176
SaqAI TTAA 3 cut(s) 630, 920, 1128
SatI GCNGC 4 cut(s) 273, 278, 512, 567
Sau3AI GATC 5 cut(s) 577, 634, 666, 777, 1076
Sau96I GGNCC 8 cut(s) 127, 140, 229, 261, 333, 354, 371, 859
ScrFI CCNGG 6 cut(s) 137, 331, 358, 369, 815, 969
SduI GDGCHC 1 cut(s) 61
SexAI ACCWGGT 1 cut(s) 813
SfaNI GCATC 1 cut(s) 1174
SfcI CTRYAG 2 cut(s) 23, 46
SfoI GGCGCC 1 cut(s) 366
Sfr274I CTCGAG 1 cut(s) 929
SinI GGWCC 3 cut(s) 127, 140, 354
SlaI CTCGAG 1 cut(s) 929
SmiMI CAYNNNNRTG 1 cut(s) 1176
SmlI CTYRAG 3 cut(s) 110, 803, 929
SmoI CTYRAG 3 cut(s) 110, 803, 929
Sse9I AATT 5 cut(s) 155, 238, 727, 795, 831
SsiI CCGC 4 cut(s) 122, 273, 326, 603
SspDI GGCGCC 1 cut(s) 364
StyD4I CCNGG 6 cut(s) 135, 329, 356, 367, 813, 967
StyI CCWWGG 3 cut(s) 204, 289, 652
TaaI ACNGT 5 cut(s) 8, 151, 458, 734, 1015
TaiI ACGT 4 cut(s) 186, 411, 531, 963
TaqI TCGA 5 cut(s) 116, 348, 480, 889, 930
TasI AATT 5 cut(s) 155, 238, 727, 795, 831
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 275
Tru1I TTAA 3 cut(s) 630, 920, 1128
Tru9I TTAA 3 cut(s) 630, 920, 1128
TseFI GTSAC 1 cut(s) 1015
TseI GCWGC 3 cut(s) 277, 511, 566
Tsp45I GTSAC 1 cut(s) 1015
TspDTI ATGAA 2 cut(s) 735, 1074
TspGWI ACGGA 4 cut(s) 94, 202, 409, 906
Tth111I GACNNNGTC 2 cut(s) 149, 358
VpaK11BI GGWCC 3 cut(s) 127, 140, 354
XapI RAATTY 1 cut(s) 155
XceI RCATGY 1 cut(s) 592
XhoI CTCGAG 1 cut(s) 929
XmnI GAANNNNTTC 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.