AT4G12230

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012477
Physical Location & Seq
Forward (+)
7284474 .. 7287578
3105 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12230.1

Sequence Viewer

Length: 1179 bp
ATGAAAGGCGTCTCTTCGACGCCCGGAGATTACGTCTACTTTAAATCTCAGGTTCCTCTTCACAAGATTCCCATTGGGACAAAGCAATGGCGTTACTATGACTTTGGTCCAAAGACTGTTCCTCCGCTCATATGTATTCCTGGAATTGCAGGAACTGCAGATGTTTACTATAAACAGATTATGGCACTCTCTATGAAGGGTTATCGGGTAATCTCAGTTGATATTCCACGGGTTTGGAATTATCATGAGTGGATTCAAGCATTTGAGAAGTTCTTGGACACCATTGATGTTCATCACGTGCATCTCTACGGTACTTCCCTTGGAGGCTTCTTAGCACAACTCTTTGCTCATCACCGTCCAAGGCGGGTTAAATCATTAGTTCTATCAAATACATATTTGGATACTCGTACTTTTGCTACTGCAATGCCATGGGCTCCTTTTGTCAGTTGGACCCCTTCTTTTTTGCTTAAACGATACGTCTTAACAGGAATTCGTGACGGACCTCATGAACCCTTTATAGCTGACTCTGTCGACTTTGCTGTTTCTCAGGTGGAGACATTGTCGAAAGATGATTTGGCTTCCCGGTTAACTTTAACTGTGGATGCTGCTTCCGTGGGATCTCTACTACTCTCTGATTCTTCAGTTACTATAATGGATACAAATGATTACTGCGCAATACCACAACATCTTAAAGATGAACTAACAGAGAGGTATCCTGAAGCAAGGAGAGCTTACTTAAAGAGTGGAGGAGATTTCCCATTCCTATCACGGCCCGACGAAGTCAATTTGCATCTACAGCTACACTTAAGGCGGGTAGGAGTAGAACCACGACCCGAAGTGGTTAAAAGCATTCCAAAAGGCGGCGCAGATGGCACAGACGGTAGCAGCCAGAGCAAGAAGAAGAGTGATGAGGAGAAGGACGACCGTAACAACAATATGCCTCAAGACTCTGGAAGCTCATCTTCAGATCAATCACCGACATTTCCAGAAAGTTCCGGAAGCTCCAACGATCCGCCTTTACCCACAGACACCATTCAGTTACTGAGTTCATCATTGAACAACCTCATATTAGTTCAACTCGCTGGTGAAGTTTATCAGTCTTGTGTGGTTTTCACATTGTGTTACTGTACATTGGTTTTGGTTCACGGTGGTGGTTTTATCTCCAGACAATTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

392

Amino Acids

43.55

Weight (kDa)

6.31

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 41 - 161 1.7e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 43 - 261 4.3e-09 Alpha/beta hydrolase family
Hydrolase_4 PF12146 79 - 161 1.3e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12230
fragaria_vesca FvH4_5g00640 FvH4_5g00640 FvH4_5g00640
malus_domestica MD04G1079900.v1.1 MD06G1074900.v1.1
prunus_persica Prupe.5G079200_v2.0.a1
pyrus_communis pycom04g07200 pycom06g05350
rosa_chinensis RchiOBHm_Chr7g0201621
rosa_laevigata RLG00000003661
rosa_multiflora Rmu_sc0004811.1_g000001
rosa_roxburghii Rroxscaffold_3G00255190
rosa_rugosa Rorug07G0065200
rosa_samantha Rh7AG191900 Rh7BG194100 Rh7CG203100 Rh7DG197100
rosa_wichuraiana Rw7G016830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 673
AccBSI CCGCTC 1 cut(s) 127
AccI GTMKAC 2 cut(s) 36, 531
AccIII TCCGGA 1 cut(s) 995
AciI CCGC 5 cut(s) 125, 364, 811, 861, 1013
AclWI GGATC 2 cut(s) 625, 1004
AcsI RAATTY 1 cut(s) 489
AcuI CTGAAG 3 cut(s) 624, 738, 948
AcvI CACGTG 1 cut(s) 298
AcyI GRCGYC 2 cut(s) 9, 20
AdeI CACNNNGTG 1 cut(s) 1119
AfaI GTAC 3 cut(s) 313, 409, 1129
AfiI CCNNNNNNNGG 1 cut(s) 860
AflII CTTAAG 1 cut(s) 805
AgsI TTSAA 3 cut(s) 257, 1057, 1076
AjnI CCWGG 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AleI CACNNNNGTG 1 cut(s) 1149
AluBI AGCT 5 cut(s) 521, 731, 799, 957, 1002
AluI AGCT 5 cut(s) 521, 731, 799, 957, 1002
Alw26I GTCTC 2 cut(s) 16, 548
AlwI GGATC 2 cut(s) 625, 1004
AlwNI CAGNNNCTG 2 cut(s) 155, 1042
Aor13HI TCCGGA 1 cut(s) 995
AoxI GGCC 1 cut(s) 770
ApeKI GCWGC 2 cut(s) 605, 885
ApoI RAATTY 1 cut(s) 489
AspLEI GCGC 2 cut(s) 674, 866
AspS9I GGNCC 4 cut(s) 107, 450, 500, 771
AsuC2I CCSGG 2 cut(s) 24, 583
AsuHPI GGTGA 3 cut(s) 344, 966, 1097
AvaII GGWCC 3 cut(s) 107, 450, 500
BanII GRGCYC 1 cut(s) 436
BbrPI CACGTG 1 cut(s) 298
BbvI GCAGC 2 cut(s) 592, 897
BccI CCATC 1 cut(s) 863
BceAI ACGGC 1 cut(s) 785
BciT130I CCWGG 1 cut(s) 141
BciVI GTATCC 3 cut(s) 394, 649, 723
BcnI CCSGG 2 cut(s) 24, 583
BcoDI GTCTC 2 cut(s) 16, 548
BfmI CTRYAG 2 cut(s) 156, 794
BfrI CTTAAG 1 cut(s) 805
BfuI GTATCC 3 cut(s) 394, 649, 723
BisI GCNGC 3 cut(s) 606, 862, 886
BlsI GCNGC 3 cut(s) 607, 863, 887
Bme1390I CCNGG 3 cut(s) 24, 141, 583
Bme18I GGWCC 3 cut(s) 107, 450, 500
BmgT120I GGNCC 4 cut(s) 107, 450, 500, 771
BmiI GGNNCC 3 cut(s) 54, 435, 452
BmrFI CCNGG 3 cut(s) 24, 141, 583
BmsI GCATC 3 cut(s) 310, 592, 799
BoxI GACNNNNGTC 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 1147
BpuEI CTTGAG 1 cut(s) 927
BpuMI CCSGG 2 cut(s) 24, 583
BsaAI YACGTR 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 291
BsaHI GRCGYC 2 cut(s) 9, 20
BsaJI CCNNGG 5 cut(s) 227, 319, 359, 428, 612
BsaWI WCCGGW 1 cut(s) 995
BsaXI ACNNNNNCTCC 6 cut(s) 18, 48, 106, 136, 545, 575
Bsc4I CCNNNNNNNGG 1 cut(s) 860
Bse3DI GCAATG 2 cut(s) 92, 429
Bse8I GATNNNNATC 1 cut(s) 291
BseAI TCCGGA 1 cut(s) 995
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 5 cut(s) 227, 319, 359, 428, 612
BseGI GGATG 1 cut(s) 607
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 860
BseMI GCAATG 2 cut(s) 92, 429
BseMII CTCAG 4 cut(s) 62, 228, 560, 1034
BseRI GAGGAG 2 cut(s) 762, 926
BseXI GCAGC 2 cut(s) 592, 897
Bsh1285I CGRYCG 1 cut(s) 925
BshFI GGCC 1 cut(s) 772
BsiEI CGRYCG 1 cut(s) 925
BsiSI CCGG 3 cut(s) 24, 583, 996
BslFI GGGAC 1 cut(s) 91
BslI CCNNNNNNNGG 1 cut(s) 860
BsmAI GTCTC 2 cut(s) 16, 548
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 849
BsnI GGCC 1 cut(s) 772
Bsp1286I GDGCHC 1 cut(s) 436
Bsp13I TCCGGA 1 cut(s) 995
Bsp1407I TGTACA 1 cut(s) 1127
Bsp143I GATC 3 cut(s) 617, 967, 1009
Bsp19I CCATGG 1 cut(s) 428
BspACI CCGC 5 cut(s) 125, 364, 811, 861, 1013
BspANI GGCC 1 cut(s) 772
BspCNI CTCAG 4 cut(s) 61, 227, 559, 1035
BspEI TCCGGA 1 cut(s) 995
BspHI TCATGA 2 cut(s) 244, 505
BspLI GGNNCC 3 cut(s) 54, 435, 452
BspMAI CTGCAG 1 cut(s) 160
BspPI GGATC 2 cut(s) 625, 1004
BspTI CTTAAG 1 cut(s) 805
BsrBI CCGCTC 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 92, 429
BsrGI TGTACA 1 cut(s) 1127
BssECI CCNNGG 5 cut(s) 227, 319, 359, 428, 612
BssMI GATC 3 cut(s) 617, 967, 1009
BssNI GRCGYC 2 cut(s) 9, 20
BssT1I CCWWGG 3 cut(s) 319, 359, 428
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 8 cut(s) 118, 311, 356, 598, 881, 926, 1127, 1148
Bst6I CTCTTC 3 cut(s) 19, 63, 896
BstACI GRCGYC 2 cut(s) 9, 20
BstAFI CTTAAG 1 cut(s) 805
BstAPI GCANNNNNTGC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 1127
BstBAI YACGTR 1 cut(s) 298
BstDEI CTNAG 5 cut(s) 48, 214, 331, 546, 1043
BstDSI CCRYGG 3 cut(s) 227, 428, 612
BstF5I GGATG 1 cut(s) 607
BstHHI GCGC 2 cut(s) 674, 866
BstKTI GATC 3 cut(s) 620, 970, 1012
BstMAI GTCTC 2 cut(s) 16, 548
BstMBI GATC 3 cut(s) 617, 967, 1009
BstMCI CGRYCG 1 cut(s) 925
BstMWI GCNNNNNNNGC 5 cut(s) 155, 728, 796, 870, 891
BstNI CCWGG 1 cut(s) 141
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 3 cut(s) 22, 139, 581
BstSFI CTRYAG 2 cut(s) 156, 794
BstV1I GCAGC 2 cut(s) 592, 897
BstX2I RGATCY 1 cut(s) 617
BstXI CCANNNNNNTGG 2 cut(s) 234, 1171
BstYI RGATCY 1 cut(s) 617
BsuI GTATCC 3 cut(s) 394, 649, 723
BsuRI GGCC 1 cut(s) 772
BtgI CCRYGG 3 cut(s) 227, 428, 612
BtsCI GGATG 1 cut(s) 607
CaiI CAGNNNCTG 2 cut(s) 155, 1042
CciI TCATGA 2 cut(s) 244, 505
CfoI GCGC 2 cut(s) 674, 866
Cfr13I GGNCC 4 cut(s) 107, 450, 500, 771
CseI GACGC 1 cut(s) 28
Csp6I GTAC 3 cut(s) 312, 408, 1128
CviAII CATG 3 cut(s) 245, 429, 506
CviQI GTAC 3 cut(s) 312, 408, 1128
DdeI CTNAG 5 cut(s) 48, 214, 331, 546, 1043
DpnI GATC 3 cut(s) 619, 969, 1011
DpnII GATC 3 cut(s) 617, 967, 1009
DraI TTTAAA 1 cut(s) 43
DraIII CACNNNGTG 1 cut(s) 1119
Eam1104I CTCTTC 3 cut(s) 19, 63, 896
EarI CTCTTC 3 cut(s) 19, 63, 896
EciI GGCGGA 1 cut(s) 1002
Eco130I CCWWGG 3 cut(s) 319, 359, 428
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 3 cut(s) 107, 450, 500
Eco57I CTGAAG 3 cut(s) 624, 738, 948
Eco72I CACGTG 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 3 cut(s) 319, 359, 428
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 3 cut(s) 319, 359, 428
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 3 cut(s) 248, 432, 509
FalI AAGNNNNNCTT 4 cut(s) 715, 747, 946, 978
FaqI GGGAC 1 cut(s) 91
FatI CATG 3 cut(s) 244, 428, 505
FauI CCCGC 2 cut(s) 357, 804
FauNDI CATATG 1 cut(s) 131
FblI GTMKAC 2 cut(s) 36, 531
Fnu4HI GCNGC 3 cut(s) 606, 862, 886
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 3 cut(s) 606, 862, 886
FspI TGCGCA 1 cut(s) 673
GlaI GCGC 2 cut(s) 673, 865
GluI GCNGC 3 cut(s) 606, 862, 886
GsuI CTGGAG 1 cut(s) 1147
HaeIII GGCC 1 cut(s) 772
HapII CCGG 3 cut(s) 24, 583, 996
HgaI GACGC 1 cut(s) 28
HhaI GCGC 2 cut(s) 674, 866
Hin1I GRCGYC 2 cut(s) 9, 20
Hin1II CATG 3 cut(s) 248, 432, 509
Hin6I GCGC 2 cut(s) 672, 864
HinP1I GCGC 2 cut(s) 672, 864
HincII GTYRAC 2 cut(s) 532, 588
HindII GTYRAC 2 cut(s) 532, 588
HinfI GANTC 5 cut(s) 67, 253, 524, 635, 947
HpaI GTTAAC 1 cut(s) 588
HpaII CCGG 3 cut(s) 24, 583, 996
HphI GGTGA 3 cut(s) 344, 966, 1097
Hpy166II GTNNAC 5 cut(s) 37, 166, 532, 588, 1144
Hpy188I TCNGA 2 cut(s) 634, 967
Hpy188III TCNNGA 9 cut(s) 245, 494, 506, 716, 944, 951, 986, 996, 1164
Hpy8I GTNNAC 5 cut(s) 37, 166, 532, 588, 1144
Hpy99I CGWCG 2 cut(s) 22, 779
HpyAV CCTTC 3 cut(s) 190, 465, 910
HpyCH4III ACNGT 8 cut(s) 118, 311, 356, 598, 881, 926, 1127, 1148
HpyCH4IV ACGT 3 cut(s) 33, 297, 477
HpyCH4V TGCA 5 cut(s) 149, 158, 301, 422, 790
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 728, 796, 870, 891
HpyF3I CTNAG 5 cut(s) 48, 214, 331, 546, 1043
HpySE526I ACGT 3 cut(s) 33, 297, 477
Hsp92I GRCGYC 2 cut(s) 9, 20
Hsp92II CATG 3 cut(s) 248, 432, 509
HspAI GCGC 2 cut(s) 672, 864
Kpn2I TCCGGA 1 cut(s) 995
KspAI GTTAAC 1 cut(s) 588
Kzo9I GATC 3 cut(s) 617, 967, 1009
LmnI GCTCC 2 cut(s) 439, 1007
Lsp1109I GCAGC 2 cut(s) 592, 897
LweI GCATC 3 cut(s) 310, 592, 799
MaeII ACGT 3 cut(s) 33, 297, 477
MaeIII GTNAC 6 cut(s) 92, 494, 643, 926, 1038, 1121
MalI GATC 3 cut(s) 619, 969, 1011
MbiI CCGCTC 1 cut(s) 127
MboI GATC 3 cut(s) 617, 967, 1009
MboII GAAGA 6 cut(s) 6, 50, 630, 910, 913, 954
MfeI CAATTG 1 cut(s) 1169
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 5 cut(s) 144, 238, 489, 784, 1169
MlyI GAGTC 2 cut(s) 518, 941
MmeI TCCRAC 2 cut(s) 428, 1029
MnlI CCTC 9 cut(s) 66, 132, 317, 513, 702, 740, 904, 951, 1073
MroI TCCGGA 1 cut(s) 995
MslI CAYNNNNRTG 1 cut(s) 1149
MspCI CTTAAG 1 cut(s) 805
MspI CCGG 3 cut(s) 24, 583, 996
MspR9I CCNGG 3 cut(s) 24, 141, 583
MunI CAATTG 1 cut(s) 1169
Mva1269I GAATGC 1 cut(s) 849
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 5 cut(s) 155, 728, 796, 870, 891
NciI CCSGG 2 cut(s) 24, 583
NcoI CCATGG 1 cut(s) 428
NdeI CATATG 1 cut(s) 131
NdeII GATC 3 cut(s) 617, 967, 1009
NlaIII CATG 3 cut(s) 248, 432, 509
NlaIV GGNNCC 3 cut(s) 54, 435, 452
NmuCI GTSAC 1 cut(s) 494
NsbI TGCGCA 1 cut(s) 673
OliI CACNNNNGTG 1 cut(s) 1149
PagI TCATGA 2 cut(s) 244, 505
PctI GAATGC 1 cut(s) 849
PfeI GAWTC 3 cut(s) 67, 253, 635
PflFI GACNNNGTC 3 cut(s) 527, 559, 779
PfoI TCCNGGA 1 cut(s) 139
PkrI GCNGC 3 cut(s) 607, 863, 887
PleI GAGTC 2 cut(s) 518, 941
PmaCI CACGTG 1 cut(s) 298
PmlI CACGTG 1 cut(s) 298
PpsI GAGTC 2 cut(s) 518, 941
Ppu21I YACGTR 1 cut(s) 298
PshAI GACNNNNGTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 139
PspCI CACGTG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 54, 435, 452
PspPI GGNCC 4 cut(s) 107, 450, 500, 771
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 2 cut(s) 155, 1042
PsuI RGATCY 1 cut(s) 617
PsyI GACNNNGTC 3 cut(s) 527, 559, 779
RsaI GTAC 3 cut(s) 313, 409, 1129
RsaNI GTAC 3 cut(s) 312, 408, 1128
RseI CAYNNNNRTG 1 cut(s) 1149
SalI GTCGAC 1 cut(s) 530
SatI GCNGC 3 cut(s) 606, 862, 886
Sau3AI GATC 3 cut(s) 617, 967, 1009
Sau96I GGNCC 4 cut(s) 107, 450, 500, 771
SchI GAGTC 2 cut(s) 518, 941
ScrFI CCNGG 3 cut(s) 24, 141, 583
SduI GDGCHC 1 cut(s) 436
SfaNI GCATC 3 cut(s) 310, 592, 799
SfcI CTRYAG 2 cut(s) 156, 794
SinI GGWCC 3 cut(s) 107, 450, 500
SmiMI CAYNNNNRTG 1 cut(s) 1149
SmlI CTYRAG 2 cut(s) 805, 942
SmoI CTYRAG 2 cut(s) 805, 942
Sse9I AATT 5 cut(s) 144, 238, 489, 784, 1169
SsiI CCGC 5 cut(s) 125, 364, 811, 861, 1013
StyD4I CCNGG 3 cut(s) 22, 139, 581
StyI CCWWGG 3 cut(s) 319, 359, 428
TaaI ACNGT 8 cut(s) 118, 311, 356, 598, 881, 926, 1127, 1148
TaiI ACGT 3 cut(s) 36, 300, 480
TaqI TCGA 3 cut(s) 17, 531, 563
TasI AATT 5 cut(s) 144, 238, 489, 784, 1169
TatI WGTACW 1 cut(s) 1127
TauI GCSGC 1 cut(s) 864
TfiI GAWTC 3 cut(s) 67, 253, 635
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 2 cut(s) 605, 885
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 6 cut(s) 17, 209, 281, 522, 711, 1038
TspGWI ACGGA 2 cut(s) 513, 601
Tth111I GACNNNGTC 3 cut(s) 527, 559, 779
Vha464I CTTAAG 1 cut(s) 805
VpaK11BI GGWCC 3 cut(s) 107, 450, 500
XapI RAATTY 1 cut(s) 489
XmiI GTMKAC 2 cut(s) 36, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.