Rorug07G0065200

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002702
Physical Location & Seq
Reverse (-)
4936392 .. 4949565
13174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0065200.1

Sequence Viewer

Length: 3660 bp
ATGTTGACCAAGTTCGAGACGAAGAGTAATAGAGTGAAGGGACTGAGCTTCCACACTAAGAGGCCGTGGATCCTCGCGAGTCTGCACAGCGGTGTGATCCAGCTATGGGACTACCGTATGGGGACTCTCATTGATCGATTCGATGAGCACGACGGCCCTGTTCGCGGTGTTCATTTCCACAAATCTCAGCCTCTCTTTGTGTCTGGAGGGGACGATTACAAGATTAAAGTTTGGAACTACAAGATGCATAGATGTCTTTTTACTCTTCTCGGGCATCTAGATTATATCCGAACCGTGCAATTTCACCATGAGTACCCATGGATTGTGAGTGCCAGTGATGACCAGACTATTCGTATATGGAACTGGCAGTCGCGCACTTGTATTTCAGTTTTGACAGGCCACAATCATTATGTTATGTGTGCCTCATTCCATCCTAAAGAAGACCTTGTCGTATCTGCCTCCTTGGATCAGACTGTCCGTGTTTGGGATATTGGTTCTCTGAAAAAGAAGTCTGTGTCCCCTGCGGATGACATACTGCGACTAAGTCAGATGAACACAGATCTTTTTGGTGGTGTTGATGCTGTTGTTAAGTATGTCTTGGAAGGGCATGACCGAGGTGTCAATTGGGCTTCATTCCACCCCAACCTGCCCCTCATTGTCTCAGGAGCAGACGATCGCCAAGTTAAATTGTGGCGGATGAATGATACTAAGGCTTGGGAAGTGGACACGTTGAGAGGTCACATGAATAATGTTTCATGTGTTATGTTTCATGCTAAGCAGGATATAATTGTATCCAACTCAGAGGACAAAAGTATCCGTGTCTGGGATGTGACAAAGCGAACTGGAATTCAAACTTTCCGACGAGAGCATGATCGCTTTTGGATTCTTTCATCTCACCCTGAAATGAATCTGTTAGCAGCAGGTCATGACAGTGGGATGATCGTCTTTAAGTTAGAGAGGGAAAGACCGGCCTTTGCCGTCAGTGGTGATTCTCTGTTCTATGCTAAAGACCGCTTTTTGAAGTTCTACGAGTTTTCAACCCAGAGAGATACACAAGTTATTCCAATTCGACGACCTGGTTCTATCACTTTAAATCAAAGTCCAAGGACCCTTTCTTACAGTCCTTCAGAAAATGCAGTTCTTGTTTGCTCAGACTTGGACGGGGGATCCTATGAGTTGTATTTCATACCCAGAGACAGCATTACTAGGGGTGACAGTGCGCAAGATGCTAAGAGAGGTGTTGGTGGTTCAGCTGTGTTTGTAGCACGGAATAGGTTTGCTGTGCTTGAGAAAAGCACAAACTCTGTCCTGGTAAAGAATTTGAGGAATGAGGTTGTTAAAAAGACTCCCCTTCCTTTTTCCGCTGATGCAATATTCTACGCTGGAACAGGTAATTTGCTGTGTAGGTCAGAGGACAAAGTGTTTATATTTGATCTCCAGCAGAGGACTGTTCTTGGTGAACTTCAAACCCCCTTCGTCAAGTATGTTGTTTGGTCTAATGACATGGAAAGTATTGCCTTGCTCAGCAAACATGCCATAATTATTGCTAGCAAGAGGCTTGTGCACCAGTGCACTCTTCATGAGACAATCCGTGTAAAGAGTGGTGGTTGGGATGACAACGGTGTTTTCATTTATTCAACTCTGAATCACATTAAATATTGTCTACCTAATGGAGATAGTGGAATAATCAGAACGCTTGATGTTCCTATCTACATTACGAAAGTTTCTGCAAATACTATCTTTTGCTTGGATCGGGATGGGAAAAATAAGGCCATAGTTATTGATGCCACAGAATACATCTTCAAGTTATCCCTTTTGAAGAAGAAATATGACAATGTTATGAGCATGATAAGGAACTCACAGCTCTGTGGGCAGGCGATGATAGCTTATCTGCAACAGAAGGGGTTCCCCGAAGTTGCCCTCCATTTTGTGAAAGATGAAAGAACTCGATTCAATCTGGCATTAGAGAGTGGGAATATTCAAATTGCGGTTGAATCTGCCACAGCAATTGATGAAAAAGATTACTGGTATAGGTTGGGTGTGGAGGCTCTTCGACAGGGCAATGCAGGTATTGTAGAGTTTGCCTACCAGAGGACCAAAAATTTTGAAAGGCTGTCTTTCCTTTATCTCATAAATGGTAATACGCAGAAACTGTCCAAGATGCTGAAAATTGCTGAGGTCAAGAATGATGTCATGGGTCAGTTTCACAATGCACTGTATCTGGGGAATGTTGAGGAGCGCATTAAGATATTGGAGAATGTTGGTCACTTGCCCCTTGCTTATATTACAGCAAAAATTCATGGGCTGCATGATGTTGCCGAAAGGCTAGCAGCTGAGTTGGGGGATAATCTTCCAACTTTGCCCGAGGGTAAAGCACCACCAACTCTTTTGACTCCCCCAACACCAATCATGTGTGGTAGTGATTGGCCACTTCTCAGAGTAATGAGAGGTATATTTGAAGGTGGGTTGGATAATATCGGCAGGGGAGCTGTGGATGAAGAGGATGAGGCTGTTGATGGTGACTGGGGTGAGGAGCTGGACATGGTTGATGTTGATGGGTTACAAAATGGAGTTGTCCTTCCAGGTTTGGAAGGTGAGGAAGTGCCTGGAGAAGATGATGGAGAGGGAGGATGGGACCTTGAAGATTTGGAGCTCCCCCCTGAAGCTGACACACCTAGGACTTCTGTTAATACTCATTCATCAGTTTTTGTTGCTCCAACTACTGGCATGCCTGTAAGCCAGATTTGGATCCAGAGATCATCTCTTGCCGCTGAGCATGCCGCTGCAGGCCATTTTGATACTGCGATGCGGTTGCTGAACAGGCAACTTGGGATTAAAAACTTCACTCCTTTGAGGCAATTGTTCCTCGATCTTCATTCTGGAAGCCACAGCTATCTACGGGCATTTTCGTCTGCTCCGGTGATATCATTAGCAGTTGAGCGTGGGTGGAATGAAACTACCACGCCAAATGTCAGAAACCCACCAGCACTTGTGTTCAATTTTTCTCAGTTGGAAGAGAAACTCAAAGCTGGTTACAAGGCTACAACTGCTGGGAAGTTCACTGAAGCTCTCAGGCTCTTTCTTAGCATTCTTCATACAATTCCTTTGATTGTTGTTGATTCAAGGAGAGAAGTTGATGAAGTTAAGGAGTTAATTGTAATAGTCAAGGAATATGTTCTGGGCTTGCAAATGGAGCTGAAGAGAAGGGAAATGAAAGATAATCCAGTACGTCAGCAGGAGCTTGCAGCTTATTTCACACACTGCAATCTTCAATTGCCTCACTTAAGGCTTGCTTTGCTTAATGCAATGACAGTTTGCTACAAGGCAAAGAACCTTGCCACTGCAGCTAACTTTGCCAGACGGCTATTGGAGACGAACCCCACCGTTGAGAGTCAGGCAAAGTTACCCAGGCAAGTGCTGCAGGCGGCAGAGAAGAATATGACTGATGCTTCTCAACTGAACTATGACTTCAGAAATCCATTTGTGACTTGTGGGGCAACATATGTACCAATTTACCGAGGACAGAAGGACGTCTCTTGCCCTTACTGTAGCTCACGGTTTGTGCCAAGCCAAGAAGGGAAGCTCTGTACTGTTTGTGATCTTGCAGTGGTTGGTGCAGATGCTTCGGGGTTGCTATGTTCTCCATCTCAGATTCGATAA

Protein Analysis

1219

Amino Acids

137.09

Weight (kDa)

6.77

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 2 - 79 3e-09 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 3 - 90 2.5e-10 WDR5 beta-propeller domain
WDR55 PF24796 3 - 168 6.2e-13 WDR55
Beta-prop_WDR3_1st PF25173 4 - 164 2.4e-32 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 4 - 162 9.8e-16 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 6 - 127 1.5e-17 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 13 - 265 3.9e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 15 - 82 8e-06 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 25 - 122 2e-07 G protein beta WD-40 repeat protein
WD40 PF00400 42 - 79 5e-06 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 48 - 168 6.4e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 49 - 166 7.6e-17 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 50 - 166 1.2e-30 WDR5 beta-propeller domain
WD40_Prp19 PF24814 60 - 168 4.7e-21 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 63 - 167 1.6e-08 TEP-1 second beta-propeller
Beta-prop_SCAP PF24017 66 - 166 7.4e-06 SCAP Beta-propeller
WD40 PF00400 84 - 121 3e-08 WD domain, G-beta repeat
EIF3I PF24805 88 - 165 4.1e-06 EIF3I
Beta-prop_WDR3_2nd PF25172 88 - 163 6e-09 WDR3 second beta-propeller domain
Beta-prop_EIPR1 PF23609 91 - 162 2.8e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 96 - 169 4.4e-13 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 108 - 169 7.7e-13 THOC3 beta-propeller domain
WD40 PF00400 126 - 163 1.1e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 183 - 304 2e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 193 - 317 2e-19 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 194 - 314 3.5e-18 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 195 - 307 8.9e-22 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 197 - 290 1.3e-07 TEP-1 second beta-propeller
WD40 PF00400 198 - 231 9.9e-07 WD domain, G-beta repeat
WD40_Gbeta PF25391 198 - 288 4.5e-06 G protein beta WD-40 repeat protein
EIF3I PF24805 199 - 287 5.5e-07 EIF3I
Beta-prop_WDR3_2nd PF25172 201 - 316 1.5e-09 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 202 - 303 3e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 205 - 316 1.3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 206 - 316 3.3e-15 THOC3 beta-propeller domain
WDR55 PF24796 207 - 315 4.2e-10 WDR55
WD40_WDHD1_1st PF24817 211 - 286 4.4e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 212 - 317 2.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 223 - 317 1.2e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 241 - 276 2e-06 WD domain, G-beta repeat
B-prop_COPA_B_2nd PF04053 340 - 584 2.4e-68 COPA/B second beta-propeller
TPR_COPA_B PF23953 613 - 770 6.1e-19 COPA/B TPR domain
COPI_C PF06957 816 - 1219 5.5e-169 Coatomer (COPI) alpha subunit C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
AatII GACGTC 1 cut(s) 3534
Acc16I TGCGCA 1 cut(s) 1221
Acc36I ACCTGC 3 cut(s) 654, 911, 2057
AccB7I CCANNNNNTGG 1 cut(s) 2723
AccI GTMKAC 1 cut(s) 1663
AccII CGCG 3 cut(s) 77, 165, 373
AclWI GGATC 9 cut(s) 64, 77, 91, 474, 1161, 1174, 1758, 2743, 2756
AcoI YGGCCR 1 cut(s) 2426
AcsI RAATTY 4 cut(s) 846, 1318, 2101, 2295
AcuI CTGAAG 5 cut(s) 1110, 2682, 3084, 3218, 3454
AcyI GRCGYC 1 cut(s) 3531
AfaI GTAC 4 cut(s) 314, 3228, 3507, 3589
AfiI CCNNNNNNNGG 6 cut(s) 106, 164, 484, 823, 2091, 2723
AflII CTTAAG 1 cut(s) 3283
AflIII ACRYGT 1 cut(s) 726
AjnI CCWGG 5 cut(s) 1075, 1308, 2581, 2605, 3407
AjuI GAANNNNNNNTTGG 2 cut(s) 1096, 1128
AleI CACNNNNGTG 1 cut(s) 90
Alw21I GWGCWC 4 cut(s) 150, 1566, 1574, 2655
Alw26I GTCTC 6 cut(s) 11, 664, 1188, 1577, 3365, 3538
Alw44I GTGCAC 2 cut(s) 1562, 1570
AlwI GGATC 9 cut(s) 64, 77, 91, 474, 1161, 1174, 1758, 2743, 2756
AlwNI CAGNNNCTG 1 cut(s) 2152
Ama87I CYCGRG 2 cut(s) 269, 2363
AoxI GGCC 7 cut(s) 62, 154, 397, 969, 1770, 2426, 2788
ApaLI GTGCAC 2 cut(s) 1562, 1570
ApeKI GCWGC 7 cut(s) 917, 2305, 2330, 2783, 3245, 3344, 3418
ApoI RAATTY 4 cut(s) 846, 1318, 2101, 2295
Asp700I GAANNNNTTC 2 cut(s) 1904, 3174
AspA2I CCTAGG 1 cut(s) 2675
AspLEI GCGC 3 cut(s) 375, 1222, 2241
AspS9I GGNCC 4 cut(s) 155, 1107, 2094, 2635
AsuHPI GGTGA 9 cut(s) 296, 887, 998, 1223, 1469, 2531, 2540, 2606, 2932
AsuNHI GCTAGC 2 cut(s) 1547, 2326
AvaI CYCGRG 2 cut(s) 269, 2363
AvaII GGWCC 3 cut(s) 1107, 2094, 2635
AvrII CCTAGG 1 cut(s) 2675
BaeGI GKGCMC 2 cut(s) 1566, 1574
BaeI ACNNNNGTAYC 6 cut(s) 796, 829, 1041, 1074, 3489, 3522
BalI TGGCCA 1 cut(s) 2428
BamHI GGATCC 3 cut(s) 69, 1166, 2748
BanII GRGCYC 1 cut(s) 2655
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 4 cut(s) 150, 1566, 1574, 2655
BbvCI CCTCAGC 1 cut(s) 2175
BbvI GCAGC 7 cut(s) 929, 2292, 2342, 2770, 3257, 3356, 3405
BccI CCATC 7 cut(s) 438, 1751, 2510, 2549, 2612, 2625, 3652
BceAI ACGGC 4 cut(s) 49, 169, 962, 3377
BcgI CGANNNNNNTGC 4 cut(s) 2794, 2828, 3606, 3640
BciT130I CCWGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
BciVI GTATCC 2 cut(s) 802, 824
BcoDI GTCTC 6 cut(s) 11, 664, 1188, 1577, 3365, 3538
BfaI CTAG 5 cut(s) 278, 1206, 1548, 2327, 2676
BfmI CTRYAG 4 cut(s) 2784, 3342, 3419, 3547
BfrI CTTAAG 1 cut(s) 3283
BfuAI ACCTGC 3 cut(s) 654, 911, 2057
BfuI GTATCC 2 cut(s) 802, 824
BglII AGATCT 1 cut(s) 559
BlnI CCTAGG 1 cut(s) 2675
BlpI GCTNAGC 3 cut(s) 774, 1523, 2772
Bme1390I CCNGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
Bme18I GGWCC 3 cut(s) 1107, 2094, 2635
BmeT110I CYCGRG 2 cut(s) 269, 2363
BmgT120I GGNCC 4 cut(s) 155, 1107, 2094, 2635
BmiI GGNNCC 6 cut(s) 71, 1109, 1168, 1907, 2636, 2750
BmrFI CCNGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
BmrI ACTGGG 1 cut(s) 2533
BmtI GCTAGC 2 cut(s) 1551, 2330
BmuI ACTGGG 1 cut(s) 2533
BpiI GAAGAC 1 cut(s) 447
BplI GAGNNNNNCTC 2 cut(s) 2746, 2778
BpmI CTGGAG 3 cut(s) 225, 1421, 2628
Bpu10I CCTNAGC 1 cut(s) 2175
Bpu1102I GCTNAGC 3 cut(s) 774, 1523, 2772
BpuEI CTTGAG 1 cut(s) 1307
Bsa29I ATCGAT 1 cut(s) 136
BsaBI GATNNNNATC 1 cut(s) 2747
BsaHI GRCGYC 1 cut(s) 3531
BsaJI CCNNGG 9 cut(s) 65, 317, 462, 613, 1103, 2364, 2675, 3407, 3517
BsaWI WCCGGW 1 cut(s) 2917
Bsc4I CCNNNNNNNGG 6 cut(s) 106, 164, 484, 823, 2091, 2723
Bse118I RCCGGY 1 cut(s) 967
Bse1I ACTGG 8 cut(s) 333, 368, 847, 1567, 2030, 2528, 2728, 3224
Bse3DI GCAATG 2 cut(s) 2068, 3312
Bse8I GATNNNNATC 1 cut(s) 2747
BseBI CCWGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
BseCI ATCGAT 1 cut(s) 136
BseDI CCNNGG 9 cut(s) 65, 317, 462, 613, 1103, 2364, 2675, 3407, 3517
BseJI GATNNNNATC 1 cut(s) 2747
BseLI CCNNNNNNNGG 6 cut(s) 106, 164, 484, 823, 2091, 2723
BseMI GCAATG 2 cut(s) 2068, 3312
BseNI ACTGG 8 cut(s) 333, 368, 847, 1567, 2030, 2528, 2728, 3224
BseRI GAGGAG 2 cut(s) 2249, 2546
BseSI GKGCMC 2 cut(s) 1566, 1574
BseXI GCAGC 7 cut(s) 929, 2292, 2342, 2770, 3257, 3356, 3405
BseYI CCCAGC 1 cut(s) 3050
BsgI GTGCAG 2 cut(s) 68, 3636
Bsh1236I CGCG 3 cut(s) 77, 165, 373
Bsh1285I CGRYCG 1 cut(s) 676
BshFI GGCC 7 cut(s) 64, 156, 399, 971, 1772, 2428, 2790
BshVI ATCGAT 1 cut(s) 136
BsiEI CGRYCG 1 cut(s) 676
BsiHKAI GWGCWC 4 cut(s) 150, 1566, 1574, 2655
BsiHKCI CYCGRG 2 cut(s) 269, 2363
BsiSI CCGG 2 cut(s) 968, 2918
BslFI GGGAC 6 cut(s) 54, 122, 136, 224, 502, 2648
BslI CCNNNNNNNGG 6 cut(s) 106, 164, 484, 823, 2091, 2723
BsmAI GTCTC 6 cut(s) 11, 664, 1188, 1577, 3365, 3538
BsmBI CGTCTC 3 cut(s) 11, 3365, 3538
BsmFI GGGAC 6 cut(s) 54, 122, 136, 224, 502, 2648
BsmI GAATGC 1 cut(s) 3087
BsnI GGCC 7 cut(s) 64, 156, 399, 971, 1772, 2428, 2790
BsoBI CYCGRG 2 cut(s) 269, 2363
Bsp1286I GDGCHC 4 cut(s) 150, 1566, 1574, 2655
Bsp1720I GCTNAGC 3 cut(s) 774, 1523, 2772
Bsp19I CCATGG 1 cut(s) 317
Bsp68I TCGCGA 1 cut(s) 77
BspANI GGCC 7 cut(s) 64, 156, 399, 971, 1772, 2428, 2790
BspDI ATCGAT 1 cut(s) 136
BspFNI CGCG 3 cut(s) 77, 165, 373
BspHI TCATGA 2 cut(s) 925, 1579
BspLI GGNNCC 6 cut(s) 71, 1109, 1168, 1907, 2636, 2750
BspMAI CTGCAG 3 cut(s) 2788, 3346, 3423
BspMI ACCTGC 3 cut(s) 654, 911, 2057
BspOI GCTAGC 2 cut(s) 1551, 2330
BspPI GGATC 9 cut(s) 64, 77, 91, 474, 1161, 1174, 1758, 2743, 2756
BspQI GCTCTTC 1 cut(s) 2055
BspTI CTTAAG 1 cut(s) 3283
BsrDI GCAATG 2 cut(s) 2068, 3312
BsrFI RCCGGY 1 cut(s) 967
BsrI ACTGG 8 cut(s) 333, 368, 847, 1567, 2030, 2528, 2728, 3224
BssAI RCCGGY 1 cut(s) 967
BssECI CCNNGG 9 cut(s) 65, 317, 462, 613, 1103, 2364, 2675, 3407, 3517
BssNI GRCGYC 1 cut(s) 3531
BssT1I CCWWGG 4 cut(s) 317, 462, 1103, 2675
Bst2UI CCWGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
Bst6I CTCTTC 7 cut(s) 17, 270, 1581, 2055, 2493, 3009, 3194
BstACI GRCGYC 1 cut(s) 3531
BstAFI CTTAAG 1 cut(s) 3283
BstAPI GCANNNNNTGC 1 cut(s) 3418
BstDSI CCRYGG 2 cut(s) 65, 317
BstENI CCTNNNNNAGG 1 cut(s) 2089
BstFNI CGCG 3 cut(s) 77, 165, 373
BstHHI GCGC 3 cut(s) 375, 1222, 2241
BstMAI GTCTC 6 cut(s) 11, 664, 1188, 1577, 3365, 3538
BstMCI CGRYCG 1 cut(s) 676
BstNI CCWGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
BstNSI RCATGY 3 cut(s) 1535, 2731, 2780
BstSCI CCNGG 5 cut(s) 1075, 1308, 2581, 2605, 3407
BstSFI CTRYAG 4 cut(s) 2784, 3342, 3419, 3547
BstSLI GKGCMC 2 cut(s) 1566, 1574
BstUI CGCG 3 cut(s) 77, 165, 373
BstV1I GCAGC 7 cut(s) 929, 2292, 2342, 2770, 3257, 3356, 3405
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 4 cut(s) 69, 559, 1166, 2748
BstYI RGATCY 4 cut(s) 69, 559, 1166, 2748
Bsu15I ATCGAT 1 cut(s) 136
BsuI GTATCC 2 cut(s) 802, 824
BsuRI GGCC 7 cut(s) 64, 156, 399, 971, 1772, 2428, 2790
BsuTUI ATCGAT 1 cut(s) 136
BtgI CCRYGG 2 cut(s) 65, 317
BtgZI GCGATG 2 cut(s) 1892, 2819
BtsI GCAGTG 3 cut(s) 3259, 3339, 3612
BtuMI TCGCGA 1 cut(s) 77
BveI ACCTGC 3 cut(s) 654, 911, 2057
CaiI CAGNNNCTG 1 cut(s) 2152
CciI TCATGA 2 cut(s) 925, 1579
CfoI GCGC 3 cut(s) 375, 1222, 2241
Cfr10I RCCGGY 1 cut(s) 967
Cfr13I GGNCC 4 cut(s) 155, 1107, 2094, 2635
ClaI ATCGAT 1 cut(s) 136
CsiI ACCWGGT 1 cut(s) 1075
Csp6I GTAC 4 cut(s) 313, 3227, 3506, 3588
CspCI CAANNNNNGTGG 2 cut(s) 1990, 2025
CviQI GTAC 4 cut(s) 313, 3227, 3506, 3588
DraI TTTAAA 1 cut(s) 1092
DrdI GACNNNNNNGTC 1 cut(s) 617
DseDI GACNNNNNNGTC 1 cut(s) 617
EaeI YGGCCR 1 cut(s) 2426
Eam1104I CTCTTC 7 cut(s) 17, 270, 1581, 2055, 2493, 3009, 3194
EarI CTCTTC 7 cut(s) 17, 270, 1581, 2055, 2493, 3009, 3194
EciI GGCGGA 1 cut(s) 709
Ecl136II GAGCTC 1 cut(s) 2653
Eco130I CCWWGG 4 cut(s) 317, 462, 1103, 2675
Eco24I GRGCYC 1 cut(s) 2655
Eco32I GATATC 1 cut(s) 2925
Eco47I GGWCC 3 cut(s) 1107, 2094, 2635
Eco53kI GAGCTC 1 cut(s) 2653
Eco57I CTGAAG 5 cut(s) 1110, 2682, 3084, 3218, 3454
Eco88I CYCGRG 2 cut(s) 269, 2363
EcoICRI GAGCTC 1 cut(s) 2653
EcoNI CCTNNNNNAGG 1 cut(s) 2089
EcoO109I RGGNCCY 2 cut(s) 1107, 2635
EcoRI GAATTC 1 cut(s) 846
EcoRII CCWGG 5 cut(s) 1075, 1308, 2581, 2605, 3407
EcoRV GATATC 1 cut(s) 2925
EcoT14I CCWWGG 4 cut(s) 317, 462, 1103, 2675
EcoT22I ATGCAT 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 2655
ErhI CCWWGG 4 cut(s) 317, 462, 1103, 2675
Esp3I CGTCTC 3 cut(s) 11, 3365, 3538
FalI AAGNNNNNCTT 8 cut(s) 429, 461, 581, 613, 2101, 2133, 3277, 3309
FaqI GGGAC 6 cut(s) 54, 122, 136, 224, 502, 2648
FauNDI CATATG 1 cut(s) 3502
FblI GTMKAC 1 cut(s) 1663
FriOI GRGCYC 1 cut(s) 2655
FspBI CTAG 5 cut(s) 278, 1206, 1548, 2327, 2676
FspI TGCGCA 1 cut(s) 1221
GlaI GCGC 3 cut(s) 374, 1221, 2240
GsaI CCCAGC 1 cut(s) 3054
GsuI CTGGAG 3 cut(s) 225, 1421, 2628
HaeIII GGCC 7 cut(s) 64, 156, 399, 971, 1772, 2428, 2790
HapII CCGG 2 cut(s) 968, 2918
HhaI GCGC 3 cut(s) 375, 1222, 2241
Hin1I GRCGYC 1 cut(s) 3531
Hin6I GCGC 3 cut(s) 373, 1220, 2239
HinP1I GCGC 3 cut(s) 373, 1220, 2239
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HpaII CCGG 2 cut(s) 968, 2918
HphI GGTGA 9 cut(s) 296, 887, 998, 1223, 1469, 2531, 2540, 2606, 2932
Hpy166II GTNNAC 7 cut(s) 6, 724, 1460, 1564, 1572, 1664, 3060
Hpy8I GTNNAC 7 cut(s) 6, 724, 1460, 1564, 1572, 1664, 3060
Hpy99I CGWCG 3 cut(s) 155, 864, 1074
HpyCH4IV ACGT 3 cut(s) 728, 3229, 3531
HpySE526I ACGT 3 cut(s) 728, 3229, 3531
Hsp92I GRCGYC 1 cut(s) 3531
HspAI GCGC 3 cut(s) 373, 1220, 2239
LguI GCTCTTC 1 cut(s) 2055
Lsp1109I GCAGC 7 cut(s) 929, 2292, 2342, 2770, 3257, 3356, 3405
MabI ACCWGGT 1 cut(s) 1075
MaeI CTAG 5 cut(s) 278, 1206, 1548, 2327, 2676
MaeII ACGT 3 cut(s) 728, 3229, 3531
MaeIII GTNAC 9 cut(s) 737, 829, 1211, 2264, 2519, 2559, 3032, 3402, 3484
MfeI CAATTG 4 cut(s) 622, 2007, 2858, 3272
MflI RGATCY 4 cut(s) 69, 559, 1166, 2748
MhlI GDGCHC 4 cut(s) 150, 1566, 1574, 2655
MlsI TGGCCA 1 cut(s) 2428
MluNI TGGCCA 1 cut(s) 2428
MlyI GAGTC 5 cut(s) 88, 118, 1339, 2386, 3400
MmeI TCCRAC 6 cut(s) 819, 883, 2378, 2448, 2741, 2991
Mox20I TGGCCA 1 cut(s) 2428
Mph1103I ATGCAT 1 cut(s) 249
MroXI GAANNNNTTC 2 cut(s) 1904, 3174
MscI TGGCCA 1 cut(s) 2428
MslI CAYNNNNRTG 2 cut(s) 90, 930
Msp20I TGGCCA 1 cut(s) 2428
MspA1I CMGCKG 6 cut(s) 90, 1253, 1364, 2333, 2771, 2783
MspCI CTTAAG 1 cut(s) 3283
MspI CCGG 2 cut(s) 968, 2918
MspR9I CCNGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
MunI CAATTG 4 cut(s) 622, 2007, 2858, 3272
Mva1269I GAATGC 1 cut(s) 3087
MvaI CCWGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
MvnI CGCG 3 cut(s) 77, 165, 373
NcoI CCATGG 1 cut(s) 317
NdeI CATATG 1 cut(s) 3502
NheI GCTAGC 2 cut(s) 1547, 2326
NlaIV GGNNCC 6 cut(s) 71, 1109, 1168, 1907, 2636, 2750
NmuCI GTSAC 6 cut(s) 737, 829, 1211, 2264, 2519, 3484
NruI TCGCGA 1 cut(s) 77
NsbI TGCGCA 1 cut(s) 1221
NsiI ATGCAT 1 cut(s) 249
NspI RCATGY 3 cut(s) 1535, 2731, 2780
OliI CACNNNNGTG 1 cut(s) 90
PaeI GCATGC 2 cut(s) 2731, 2780
PagI TCATGA 2 cut(s) 925, 1579
PciSI GCTCTTC 1 cut(s) 2055
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 1 cut(s) 3087
PdmI GAANNNNTTC 2 cut(s) 1904, 3174
PfeI GAWTC 9 cut(s) 138, 883, 907, 989, 1645, 1950, 1994, 3119, 3652
PflFI GACNNNGTC 2 cut(s) 446, 543
PflMI CCANNNNNTGG 1 cut(s) 2723
Ple19I CGATCG 1 cut(s) 676
PleI GAGTC 5 cut(s) 87, 118, 1339, 2386, 3399
PpsI GAGTC 5 cut(s) 87, 118, 1339, 2386, 3399
PpuMI RGGWCCY 2 cut(s) 1107, 2635
Psp124BI GAGCTC 1 cut(s) 2655
Psp5II RGGWCCY 2 cut(s) 1107, 2635
Psp6I CCWGG 5 cut(s) 1075, 1308, 2581, 2605, 3407
PspFI CCCAGC 1 cut(s) 3050
PspGI CCWGG 5 cut(s) 1075, 1308, 2581, 2605, 3407
PspN4I GGNNCC 6 cut(s) 71, 1109, 1168, 1907, 2636, 2750
PspPI GGNCC 4 cut(s) 155, 1107, 2094, 2635
PspPPI RGGWCCY 2 cut(s) 1107, 2635
PstI CTGCAG 3 cut(s) 2788, 3346, 3423
PstNI CAGNNNCTG 1 cut(s) 2152
PsuI RGATCY 4 cut(s) 69, 559, 1166, 2748
PsyI GACNNNGTC 2 cut(s) 446, 543
PvuI CGATCG 1 cut(s) 676
PvuII CAGCTG 2 cut(s) 1253, 2333
RruI TCGCGA 1 cut(s) 77
RsaI GTAC 4 cut(s) 314, 3228, 3507, 3589
RsaNI GTAC 4 cut(s) 313, 3227, 3506, 3588
RseI CAYNNNNRTG 2 cut(s) 90, 930
SacI GAGCTC 1 cut(s) 2655
SapI GCTCTTC 1 cut(s) 2055
Sau96I GGNCC 4 cut(s) 155, 1107, 2094, 2635
SchI GAGTC 5 cut(s) 88, 118, 1339, 2386, 3400
ScrFI CCNGG 5 cut(s) 1077, 1310, 2583, 2607, 3409
SduI GDGCHC 4 cut(s) 150, 1566, 1574, 2655
SexAI ACCWGGT 1 cut(s) 1075
SfcI CTRYAG 4 cut(s) 2784, 3342, 3419, 3547
SinI GGWCC 3 cut(s) 1107, 2094, 2635
SmiMI CAYNNNNRTG 2 cut(s) 90, 930
SmlI CTYRAG 2 cut(s) 1286, 3283
SmoI CTYRAG 2 cut(s) 1286, 3283
SphI GCATGC 2 cut(s) 2731, 2780
SspI AATATT 3 cut(s) 1374, 1658, 1978
SspMI CTAG 5 cut(s) 278, 1206, 1548, 2327, 2676
SstI GAGCTC 1 cut(s) 2655
StyD4I CCNGG 5 cut(s) 1075, 1308, 2581, 2605, 3407
StyI CCWWGG 4 cut(s) 317, 462, 1103, 2675
TaiI ACGT 3 cut(s) 731, 3232, 3534
TaqI TCGA 8 cut(s) 15, 136, 141, 1069, 1948, 2053, 2868, 3655
TaqII GACCGA 1 cut(s) 627
TatI WGTACW 1 cut(s) 3587
TauI GCSGC 3 cut(s) 2771, 2783, 3428
TfiI GAWTC 9 cut(s) 138, 883, 907, 989, 1645, 1950, 1994, 3119, 3652
TseFI GTSAC 6 cut(s) 737, 829, 1211, 2264, 2519, 3484
TseI GCWGC 7 cut(s) 917, 2305, 2330, 2783, 3245, 3344, 3418
Tsp45I GTSAC 6 cut(s) 737, 829, 1211, 2264, 2519, 3484
TspGWI ACGGA 4 cut(s) 467, 806, 1282, 1580
Tth111I GACNNNGTC 2 cut(s) 446, 543
Van91I CCANNNNNTGG 1 cut(s) 2723
Vha464I CTTAAG 1 cut(s) 3283
VneI GTGCAC 2 cut(s) 1562, 1570
VpaK11BI GGWCC 3 cut(s) 1107, 2094, 2635
XagI CCTNNNNNAGG 1 cut(s) 2089
XapI RAATTY 4 cut(s) 846, 1318, 2101, 2295
XbaI TCTAGA 1 cut(s) 277
XceI RCATGY 3 cut(s) 1535, 2731, 2780
XcmI CCANNNNNNNNNTGG 1 cut(s) 3364
XmaJI CCTAGG 1 cut(s) 2675
XmiI GTMKAC 1 cut(s) 1663
XmnI GAANNNNTTC 2 cut(s) 1904, 3174
XspI CTAG 5 cut(s) 278, 1206, 1548, 2327, 2676
ZraI GACGTC 1 cut(s) 3532
Zsp2I ATGCAT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.