pycom06g05340

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002702
Physical Location & Seq
Forward (+)
7739667 .. 7739900
234 bp
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UTR
Exon/CDS
Intron
pycom06g05340.1

Sequence Viewer

Length: 234 bp
ATGGCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTCCGCGACGCCTTCAAGGTTCTCGACAAGGACTCCACCGGGTTCGTCTCCGTCTCGGAGCTCCGGCACATCCTCACCAGCATCGGCGAGAAATTGGAGCCCTCCGAATTTGACGAGTGGATCCGAGAGGTGGATGTCGGGTCCGATGGGACGATCAAATACGAGGACTTCATTGCCAGAATGGTGGCCAAGTGA

Protein Analysis

78

Amino Acids

8.89

Weight (kDa)

5.08

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 12 - 74 6.1e-17 EF-hand domain pair
EF-hand_6 PF13405 13 - 42 1.6e-08 EF-hand domain
EF-hand_1 PF00036 14 - 40 1.8e-06 EF hand domain
EF-hand_9 PF14658 17 - 74 1.2e-06 EF-hand domain
EF-hand_8 PF13833 27 - 75 1e-05 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 45
AciI CCGC 1 cut(s) 43
AclWI GGATC 2 cut(s) 154, 167
AcoI YGGCCR 2 cut(s) 3, 225
AcsI RAATTY 1 cut(s) 146
AcyI GRCGYC 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 1 cut(s) 55
AluBI AGCT 2 cut(s) 40, 100
AluI AGCT 2 cut(s) 40, 100
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 91, 97
AlwI GGATC 2 cut(s) 154, 167
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 2 cut(s) 3, 225
ApoI RAATTY 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 180
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 1 cut(s) 180
BalI TGGCCA 2 cut(s) 5, 227
BamHI GGATCC 1 cut(s) 159
BanII GRGCYC 3 cut(s) 27, 102, 141
Bbv12I GWGCWC 1 cut(s) 102
BccI CCATC 1 cut(s) 179
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 91, 97
Bme1390I CCNGG 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 180
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 180
BmiI GGNNCC 3 cut(s) 138, 161, 181
BmrFI CCNGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 129
BpuMI CCSGG 1 cut(s) 79
BsaHI GRCGYC 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 210
BseGI GGATG 2 cut(s) 108, 178
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 45
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 2 cut(s) 5, 227
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 2 cut(s) 78, 103
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 2 cut(s) 91, 97
BsmBI CGTCTC 2 cut(s) 91, 97
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 2 cut(s) 5, 227
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 3 cut(s) 27, 102, 141
Bsp143I GATC 3 cut(s) 31, 159, 192
BspACI CCGC 1 cut(s) 43
BspANI GGCC 2 cut(s) 5, 227
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 3 cut(s) 138, 161, 181
BspPI GGATC 2 cut(s) 154, 167
BsrDI GCAATG 1 cut(s) 210
BssMI GATC 3 cut(s) 31, 159, 192
BssNI GRCGYC 1 cut(s) 48
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 38
BstF5I GGATG 2 cut(s) 108, 178
BstFNI CGCG 1 cut(s) 45
BstKTI GATC 3 cut(s) 34, 162, 195
BstMAI GTCTC 2 cut(s) 91, 97
BstMBI GATC 3 cut(s) 31, 159, 192
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 16, 42
BstSCI CCNGG 1 cut(s) 77
BstUI CGCG 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 159
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 2 cut(s) 5, 227
BtsCI GGATG 2 cut(s) 108, 178
Cac8I GCNNGC 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 180
CseI GACGC 1 cut(s) 56
CviAII CATG 1 cut(s) 13
CviJI RGCY 7 cut(s) 5, 19, 25, 40, 100, 139, 227
CviKI_1 RGCY 7 cut(s) 5, 19, 25, 40, 100, 139, 227
DpnI GATC 3 cut(s) 33, 161, 194
DpnII GATC 3 cut(s) 31, 159, 192
EaeI YGGCCR 2 cut(s) 3, 225
Ecl136II GAGCTC 1 cut(s) 100
Eco24I GRGCYC 3 cut(s) 27, 102, 141
Eco47I GGWCC 1 cut(s) 180
Eco53kI GAGCTC 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 100
EcoT38I GRGCYC 3 cut(s) 27, 102, 141
Esp3I CGTCTC 2 cut(s) 91, 97
FaeI CATG 1 cut(s) 16
FaiI YATR 1 cut(s) 14
FaqI GGGAC 1 cut(s) 202
FatI CATG 1 cut(s) 12
FokI GGATG 2 cut(s) 95, 185
FriOI GRGCYC 3 cut(s) 27, 102, 141
HaeIII GGCC 2 cut(s) 5, 227
HapII CCGG 2 cut(s) 78, 103
HgaI GACGC 1 cut(s) 56
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 1 cut(s) 16
HinfI GANTC 1 cut(s) 71
HpaII CCGG 2 cut(s) 78, 103
HphI GGTGA 1 cut(s) 106
Hpy188I TCNGA 4 cut(s) 97, 145, 164, 184
Hpy188III TCNNGA 1 cut(s) 62
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 37, 61
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 42
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 1 cut(s) 16
Kzo9I GATC 3 cut(s) 31, 159, 192
LmnI GCTCC 4 cut(s) 45, 97, 105, 136
LpnPI CCDG 5 cut(s) 50, 91, 116, 130, 229
LweI GCATC 1 cut(s) 129
MalI GATC 3 cut(s) 33, 161, 194
MboI GATC 3 cut(s) 31, 159, 192
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 3 cut(s) 27, 102, 141
MlsI TGGCCA 2 cut(s) 5, 227
MluCI AATT 2 cut(s) 131, 146
MluNI TGGCCA 2 cut(s) 5, 227
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 4 cut(s) 122, 151, 160, 196
Mox20I TGGCCA 2 cut(s) 5, 227
MscI TGGCCA 2 cut(s) 5, 227
Msp20I TGGCCA 2 cut(s) 5, 227
MspI CCGG 2 cut(s) 78, 103
MspR9I CCNGG 1 cut(s) 79
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 16, 42
NciI CCSGG 1 cut(s) 79
NdeII GATC 3 cut(s) 31, 159, 192
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 3 cut(s) 138, 161, 181
Ple19I CGATCG 1 cut(s) 34
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 102
PspN4I GGNNCC 3 cut(s) 138, 161, 181
PspPI GGNCC 1 cut(s) 180
PsuI RGATCY 1 cut(s) 159
PvuI CGATCG 1 cut(s) 34
SacI GAGCTC 1 cut(s) 102
Sau3AI GATC 3 cut(s) 31, 159, 192
Sau96I GGNCC 1 cut(s) 180
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 79
SduI GDGCHC 3 cut(s) 27, 102, 141
SetI ASST 4 cut(s) 42, 60, 102, 171
SfaNI GCATC 1 cut(s) 129
SinI GGWCC 1 cut(s) 180
Sse9I AATT 2 cut(s) 131, 146
SsiI CCGC 1 cut(s) 43
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 77
TaqI TCGA 2 cut(s) 30, 63
TasI AATT 2 cut(s) 131, 146
TspDTI ATGAA 2 cut(s) 29, 199
TspGWI ACGGA 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 180
XapI RAATTY 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.