Rw5G028070

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002702
Physical Location & Seq
Reverse (-)
42123120 .. 42123506
387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028070.1

Sequence Viewer

Length: 387 bp
ATGGAACCGTGGAAGGGTTACTATCATCCGACCCATGCCCAACTTAAATCCATAGTCGCCGAGGAGAAGCTGACCGCCCCCTTCAACTTCAAACGCTTCCTGGAGCTGATGGGGATGCACATGAAGCCTGAGCCCTTCAATCGCCAGCTCCGCGACGCCTTCAAGGTCCTTGACAAGGACGCCACCGACTTCGTCTCCTTCTCGGAGCTCCGGTACATCCTTACCAGCATTGGCGAGAAATTGGAGGTCTTCCCTAATAACATTGAGGCCGGAGTCGTTGTTGGTGGCGCTGGAGACGAGTTTCCAGTAGAAATGCGCTGGCGTCGTTGTTGGATAGTGTCACACCCTAACCCGAAAATCTGCAATTTTTTTTTCTTTCCAGAGTAA

Protein Analysis

128

Amino Acids

15.0

Weight (kDa)

6.43

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 49 - 78 5.4e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 153
AciI CCGC 2 cut(s) 75, 151
AcyI GRCGYC 3 cut(s) 156, 180, 322
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 2 cut(s) 14, 175
AgsI TTSAA 4 cut(s) 85, 91, 139, 163
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 4 cut(s) 70, 106, 148, 208
AluI AGCT 4 cut(s) 70, 106, 148, 208
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 2 cut(s) 199, 288
AoxI GGCC 1 cut(s) 267
AspLEI GCGC 2 cut(s) 290, 318
AspS9I GGNCC 1 cut(s) 166
AvaII GGWCC 1 cut(s) 166
BanII GRGCYC 2 cut(s) 135, 210
BbsI GAAGAC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 210
BccI CCATC 1 cut(s) 103
BciT130I CCWGG 1 cut(s) 101
BcoDI GTCTC 2 cut(s) 199, 288
BfoI RGCGCY 1 cut(s) 291
Bme1390I CCNGG 1 cut(s) 101
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 241
BpmI CTGGAG 2 cut(s) 122, 312
Bpu10I CCTNAGC 1 cut(s) 129
BsaHI GRCGYC 3 cut(s) 156, 180, 322
BsaJI CCNNGG 2 cut(s) 8, 60
BsaWI WCCGGW 1 cut(s) 210
BsaXI ACNNNNNCTCC 6 cut(s) 179, 197, 209, 227, 264, 294
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 175
Bse1I ACTGG 1 cut(s) 305
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 2 cut(s) 8, 60
BseGI GGATG 3 cut(s) 25, 120, 216
BseLI CCNNNNNNNGG 2 cut(s) 14, 175
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 2 cut(s) 211, 270
BslI CCNNNNNNNGG 2 cut(s) 14, 175
BsmAI GTCTC 2 cut(s) 199, 288
BsmBI CGTCTC 2 cut(s) 199, 288
BsnI GGCC 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 135, 210
BspACI CCGC 2 cut(s) 75, 151
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 121
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 6
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 2 cut(s) 8, 60
BssNI GRCGYC 3 cut(s) 156, 180, 322
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 1 cut(s) 9
BstACI GRCGYC 3 cut(s) 156, 180, 322
BstC8I GCNNGC 2 cut(s) 146, 320
BstDEI CTNAG 1 cut(s) 129
BstDSI CCRYGG 1 cut(s) 8
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 3 cut(s) 25, 120, 216
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 2 cut(s) 290, 318
BstMAI GTCTC 2 cut(s) 199, 288
BstMWI GCNNNNNNNGC 2 cut(s) 124, 150
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 99
BstUI CGCG 1 cut(s) 153
BstV2I GAAGAC 1 cut(s) 241
BsuRI GGCC 1 cut(s) 269
BtgI CCRYGG 1 cut(s) 8
BtsCI GGATG 3 cut(s) 25, 120, 216
Cac8I GCNNGC 2 cut(s) 146, 320
CfoI GCGC 2 cut(s) 290, 318
Cfr13I GGNCC 1 cut(s) 166
CseI GACGC 3 cut(s) 164, 188, 311
Csp6I GTAC 1 cut(s) 214
CviAII CATG 2 cut(s) 35, 121
CviJI RGCY 7 cut(s) 70, 106, 127, 133, 148, 208, 269
CviKI_1 RGCY 7 cut(s) 70, 106, 127, 133, 148, 208, 269
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 2 cut(s) 135, 210
Eco47I GGWCC 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 135, 210
Esp3I CGTCTC 2 cut(s) 199, 288
FaeI CATG 2 cut(s) 38, 124
FaiI YATR 3 cut(s) 36, 53, 122
FatI CATG 2 cut(s) 34, 120
FokI GGATG 3 cut(s) 12, 127, 203
FriOI GRGCYC 2 cut(s) 135, 210
GlaI GCGC 2 cut(s) 289, 317
GsuI CTGGAG 2 cut(s) 122, 312
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 1 cut(s) 269
HapII CCGG 2 cut(s) 211, 270
HgaI GACGC 3 cut(s) 164, 188, 311
HhaI GCGC 2 cut(s) 290, 318
Hin1I GRCGYC 3 cut(s) 156, 180, 322
Hin1II CATG 2 cut(s) 38, 124
Hin6I GCGC 2 cut(s) 288, 316
HinP1I GCGC 2 cut(s) 288, 316
HinfI GANTC 1 cut(s) 273
HpaII CCGG 2 cut(s) 211, 270
Hpy188I TCNGA 2 cut(s) 30, 205
Hpy188III TCNNGA 1 cut(s) 380
Hpy99I CGWCG 2 cut(s) 158, 327
HpyAV CCTTC 5 cut(s) 7, 91, 145, 169, 208
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 118, 363
HpyF10VI GCNNNNNNNGC 2 cut(s) 124, 150
HpyF3I CTNAG 1 cut(s) 129
Hsp92I GRCGYC 3 cut(s) 156, 180, 322
Hsp92II CATG 2 cut(s) 38, 124
HspAI GCGC 2 cut(s) 288, 316
LmnI GCTCC 4 cut(s) 103, 153, 205, 213
LweI GCATC 1 cut(s) 105
MaeIII GTNAC 2 cut(s) 17, 339
MboII GAAGA 1 cut(s) 241
MhlI GDGCHC 2 cut(s) 135, 210
MluCI AATT 2 cut(s) 239, 364
MlyI GAGTC 1 cut(s) 282
MmeI TCCRAC 2 cut(s) 53, 311
MnlI CCTC 3 cut(s) 55, 238, 259
MseI TTAA 1 cut(s) 45
MspI CCGG 2 cut(s) 211, 270
MspR9I CCNGG 1 cut(s) 101
MvaI CCWGG 1 cut(s) 101
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 124, 150
NlaIII CATG 2 cut(s) 38, 124
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 339
PflFI GACNNNGTC 1 cut(s) 191
PfoI TCCNGGA 1 cut(s) 99
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 166
Psp124BI GAGCTC 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 166
PspPPI RGGWCCY 1 cut(s) 166
PsyI GACNNNGTC 1 cut(s) 191
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 45
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 2 cut(s) 135, 210
SetI ASST 6 cut(s) 72, 108, 150, 168, 210, 249
SfaNI GCATC 1 cut(s) 105
SinI GGWCC 1 cut(s) 166
Sse9I AATT 2 cut(s) 239, 364
SsiI CCGC 2 cut(s) 75, 151
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 9
TasI AATT 2 cut(s) 239, 364
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TseFI GTSAC 1 cut(s) 339
Tsp45I GTSAC 1 cut(s) 339
TspDTI ATGAA 1 cut(s) 137
Tth111I GACNNNGTC 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 166
XagI CCTNNNNNAGG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.