Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002702

Physical Location & Seq

Genomic Coordinates Forward (+)

10973032 .. 10973475

Gene Structure 444 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1079800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 444 bp

ATGGGCAAAGATCTGAGCGATGACCAAGTATCGTCGATGAAGGAGGCGTTCACGCTCTTCGACACCGACAACGACGGCAAGATTGCTCCGTCGGAGCTCGGGATCCTAATGCGATCGCTCGGCGGCAACCCGACCCAGGCCCAACTCAAATCCATAGTCGCCGAGGAGAAGATCACCGCCCCCTTCGACTTCCCTCGCTTCCTCGACCTCATGGCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTCCGCGACGCCTTCAAGGTCCTCGACAAGGACTCAACCGGCTTCGTGTCCGTGTCGGAGCTCCGGCACATCCTCACCAGCATCGGCGAGAAATTGGAGCCGTCCGAGTTCGACGAGTGGATCCGGGAGGTGGATGTCGGGTCCGATGGCAAGATCAAATACGAGGACTTCATTGCCAGAATGGTGGCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko03010 ko04626 map03010 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_6 EF-hand_7

Protein Analysis

148

Amino Acids

16.56

Weight (kDa)

4.8

Isoelectric Point (pI)

33.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	12 - 54	2.5e-06	EF-hand domain pair
EF-hand_1	PF00036	13 - 40	3.2e-06	EF hand domain
EF-hand_6	PF13405	13 - 41	5.2e-07	EF-hand domain
EF-hand_7	PF13499	82 - 144	1.7e-16	EF-hand domain pair
EF-hand_6	PF13405	83 - 112	5.5e-08	EF-hand domain
EF-hand_1	PF00036	84 - 110	6.1e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002702)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12310 AT1G62820
fragaria_vesca	FvH4_5g00650 FvH4_7g20700
malus_domestica	MD03G1151100.v1.1 MD04G1079800.v1.1 MD06G1074600.v1.1 MD07G1265800.v1.1 MD11G1152700.v1.1 MD17G1283300.v1.1
prunus_persica	Prupe.5G079300_v2.0.a1
pyrus_communis	pycom06g05340
rosa_chinensis	RchiOBHm_Chr1g0364931 RchiOBHm_Chr3g0455101 RchiOBHm_Chr5g0045151 RchiOBHm_Chr6g0299731 RchiOBHm_Chr7g0201611
rosa_laevigata	RLG00000027458
rosa_multiflora	Rmu_sc0002502.1_g000006 Rmu_sc0002895.1_g000017 Rmu_sc0006410.1_g000028 Rmu_sc0007033.1_g000001 Rmu_sc0011159.1_g000005 Rmu_ssc0000112.1_g000026 Rmu_ssc0000304.1_g000001
rosa_roxburghii	Rroxscaffold_3G00255200 Rroxscaffold_4G00292060
rosa_rugosa	Rorug01G0318600 Rorug07G0065200
rosa_samantha	Rh1BG288500 Rh1CG305200 Rh1DG320100 Rh2CG069200 Rh7AG191700 Rh7BG194000 Rh7DG197000
rosa_wichuraiana	Rw1G029000 Rw5G028070 Rw7G016820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	255
AciI	CCGC	3 cut(s)	123, 177, 253
AclWI	GGATC	4 cut(s)	97, 110, 364, 377
AcoI	YGGCCR	2 cut(s)	213, 435
AcyI	GRCGYC	1 cut(s)	258
AfiI	CCNNNNNNNGG	3 cut(s)	136, 277, 379
AgsI	TTSAA	1 cut(s)	265
AjnI	CCWGG	1 cut(s)	135
AluBI	AGCT	3 cut(s)	97, 250, 310
AluI	AGCT	3 cut(s)	97, 250, 310
Alw21I	GWGCWC	2 cut(s)	99, 312
AlwI	GGATC	4 cut(s)	97, 110, 364, 377
Ama87I	CYCGRG	2 cut(s)	98, 230
AoxI	GGCC	3 cut(s)	138, 213, 435
ArsI	GACNNNNNNTTYG	2 cut(s)	141, 173
AsiSI	GCGATCGC	1 cut(s)	116
AspS9I	GGNCC	3 cut(s)	139, 268, 390
AsuC2I	CCSGG	1 cut(s)	374
AsuHPI	GGTGA	2 cut(s)	166, 316
AvaI	CYCGRG	2 cut(s)	98, 230
AvaII	GGWCC	2 cut(s)	268, 390
BalI	TGGCCA	2 cut(s)	215, 437
BamHI	GGATCC	2 cut(s)	102, 369
BanII	GRGCYC	3 cut(s)	99, 237, 312
Bbv12I	GWGCWC	2 cut(s)	99, 312
BccI	CCATC	1 cut(s)	389
BceAI	ACGGC	2 cut(s)	91, 334
BciT130I	CCWGG	1 cut(s)	137
BcnI	CCSGG	1 cut(s)	374
BglII	AGATCT	1 cut(s)	10
BisI	GCNGC	1 cut(s)	124
BlsI	GCNGC	1 cut(s)	125
Bme1390I	CCNGG	2 cut(s)	137, 374
Bme18I	GGWCC	2 cut(s)	268, 390
BmeT110I	CYCGRG	2 cut(s)	98, 230
BmgT120I	GGNCC	3 cut(s)	139, 268, 390
BmiI	GGNNCC	4 cut(s)	104, 348, 371, 391
BmrFI	CCNGG	2 cut(s)	137, 374
BmsI	GCATC	1 cut(s)	339
BpuMI	CCSGG	1 cut(s)	374
BsaHI	GRCGYC	1 cut(s)	258
BsaJI	CCNNGG	2 cut(s)	135, 162
Bsc4I	CCNNNNNNNGG	3 cut(s)	136, 277, 379
Bse118I	RCCGGY	1 cut(s)	287
Bse3DI	GCAATG	1 cut(s)	420
BseBI	CCWGG	1 cut(s)	137
BseDI	CCNNGG	2 cut(s)	135, 162
BseGI	GGATG	2 cut(s)	318, 388
BseLI	CCNNNNNNNGG	3 cut(s)	136, 277, 379
BseMI	GCAATG	1 cut(s)	420
BseMII	CTCAG	1 cut(s)	5
BseRI	GAGGAG	1 cut(s)	179
Bsh1236I	CGCG	1 cut(s)	255
Bsh1285I	CGRYCG	2 cut(s)	116, 244
BshFI	GGCC	3 cut(s)	140, 215, 437
BsiEI	CGRYCG	2 cut(s)	116, 244
BsiHKAI	GWGCWC	2 cut(s)	99, 312
BsiHKCI	CYCGRG	2 cut(s)	98, 230
BsiSI	CCGG	3 cut(s)	288, 313, 373
BslI	CCNNNNNNNGG	3 cut(s)	136, 277, 379
BsnI	GGCC	3 cut(s)	140, 215, 437
BsoBI	CYCGRG	2 cut(s)	98, 230
Bsp1286I	GDGCHC	3 cut(s)	99, 237, 312
Bsp143I	GATC	7 cut(s)	10, 102, 113, 171, 241, 369, 402
BspACI	CCGC	3 cut(s)	123, 177, 253
BspANI	GGCC	3 cut(s)	140, 215, 437
BspCNI	CTCAG	1 cut(s)	6
BspFNI	CGCG	1 cut(s)	255
BspLI	GGNNCC	4 cut(s)	104, 348, 371, 391
BspPI	GGATC	4 cut(s)	97, 110, 364, 377
BspQI	GCTCTTC	1 cut(s)	62
BsrDI	GCAATG	1 cut(s)	420
BsrFI	RCCGGY	1 cut(s)	287
BssAI	RCCGGY	1 cut(s)	287
BssECI	CCNNGG	2 cut(s)	135, 162
BssMI	GATC	7 cut(s)	10, 102, 113, 171, 241, 369, 402
BssNI	GRCGYC	1 cut(s)	258
Bst2UI	CCWGG	1 cut(s)	137
Bst6I	CTCTTC	1 cut(s)	62
BstACI	GRCGYC	1 cut(s)	258
BstC8I	GCNNGC	1 cut(s)	248
BstDEI	CTNAG	1 cut(s)	14
BstENI	CCTNNNNNAGG	1 cut(s)	275
BstF5I	GGATG	2 cut(s)	318, 388
BstFNI	CGCG	1 cut(s)	255
BstKTI	GATC	7 cut(s)	13, 105, 116, 174, 244, 372, 405
BstMBI	GATC	7 cut(s)	10, 102, 113, 171, 241, 369, 402
BstMCI	CGRYCG	2 cut(s)	116, 244
BstMWI	GCNNNNNNNGC	2 cut(s)	226, 252
BstNI	CCWGG	1 cut(s)	137
BstSCI	CCNGG	2 cut(s)	135, 372
BstUI	CGCG	1 cut(s)	255
BstX2I	RGATCY	3 cut(s)	10, 102, 369
BstXI	CCANNNNNNTGG	1 cut(s)	433
BstYI	RGATCY	3 cut(s)	10, 102, 369
BsuRI	GGCC	3 cut(s)	140, 215, 437
BtgZI	GCGATG	1 cut(s)	33
BtsCI	GGATG	2 cut(s)	318, 388
Cac8I	GCNNGC	1 cut(s)	248
Cfr10I	RCCGGY	1 cut(s)	287
Cfr13I	GGNCC	3 cut(s)	139, 268, 390
CseI	GACGC	1 cut(s)	266
CviAII	CATG	2 cut(s)	211, 223
DdeI	CTNAG	1 cut(s)	14
DpnI	GATC	7 cut(s)	12, 104, 115, 173, 243, 371, 404
DpnII	GATC	7 cut(s)	10, 102, 113, 171, 241, 369, 402
EaeI	YGGCCR	2 cut(s)	213, 435
Eam1104I	CTCTTC	1 cut(s)	62
EarI	CTCTTC	1 cut(s)	62
Ecl136II	GAGCTC	2 cut(s)	97, 310
Eco24I	GRGCYC	3 cut(s)	99, 237, 312
Eco47I	GGWCC	2 cut(s)	268, 390
Eco53kI	GAGCTC	2 cut(s)	97, 310
Eco88I	CYCGRG	2 cut(s)	98, 230
EcoICRI	GAGCTC	2 cut(s)	97, 310
EcoNI	CCTNNNNNAGG	1 cut(s)	275
EcoO109I	RGGNCCY	1 cut(s)	268
EcoRII	CCWGG	1 cut(s)	135
EcoT38I	GRGCYC	3 cut(s)	99, 237, 312
FaeI	CATG	2 cut(s)	214, 226
FaiI	YATR	3 cut(s)	155, 212, 224
FatI	CATG	2 cut(s)	210, 222
Fnu4HI	GCNGC	1 cut(s)	124
FokI	GGATG	2 cut(s)	305, 395
FriOI	GRGCYC	3 cut(s)	99, 237, 312
Fsp4HI	GCNGC	1 cut(s)	124
GluI	GCNGC	1 cut(s)	124
HaeIII	GGCC	3 cut(s)	140, 215, 437
HapII	CCGG	3 cut(s)	288, 313, 373
HgaI	GACGC	1 cut(s)	266
Hin1I	GRCGYC	1 cut(s)	258
Hin1II	CATG	2 cut(s)	214, 226
HinfI	GANTC	1 cut(s)	281
HpaII	CCGG	3 cut(s)	288, 313, 373
HphI	GGTGA	2 cut(s)	166, 316
Hpy166II	GTNNAC	1 cut(s)	51
Hpy188I	TCNGA	5 cut(s)	15, 94, 307, 355, 394
Hpy188III	TCNNGA	1 cut(s)	100
Hpy8I	GTNNAC	1 cut(s)	51
Hpy99I	CGWCG	5 cut(s)	37, 77, 94, 260, 365
HpyAV	CCTTC	4 cut(s)	34, 193, 247, 271
HpyF10VI	GCNNNNNNNGC	2 cut(s)	226, 252
HpyF3I	CTNAG	1 cut(s)	14
Hsp92I	GRCGYC	1 cut(s)	258
Hsp92II	CATG	2 cut(s)	214, 226
Kzo9I	GATC	7 cut(s)	10, 102, 113, 171, 241, 369, 402
LguI	GCTCTTC	1 cut(s)	62
LmnI	GCTCC	6 cut(s)	91, 94, 255, 307, 315, 346
LpnPI	CCDG	8 cut(s)	122, 149, 260, 301, 326, 340, 386, 439
LweI	GCATC	1 cut(s)	339
MalI	GATC	7 cut(s)	12, 104, 115, 173, 243, 371, 404
MboI	GATC	7 cut(s)	10, 102, 113, 171, 241, 369, 402
MboII	GAAGA	2 cut(s)	49, 181
MflI	RGATCY	3 cut(s)	10, 102, 369
MhlI	GDGCHC	3 cut(s)	99, 237, 312
MlsI	TGGCCA	2 cut(s)	215, 437
MluCI	AATT	1 cut(s)	341
MluNI	TGGCCA	2 cut(s)	215, 437
MlyI	GAGTC	1 cut(s)	275
MmeI	TCCRAC	2 cut(s)	72, 285
MnlI	CCTC	9 cut(s)	37, 157, 204, 212, 218, 281, 332, 370, 406
Mox20I	TGGCCA	2 cut(s)	215, 437
MscI	TGGCCA	2 cut(s)	215, 437
Msp20I	TGGCCA	2 cut(s)	215, 437
MspI	CCGG	3 cut(s)	288, 313, 373
MspR9I	CCNGG	2 cut(s)	137, 374
MvaI	CCWGG	1 cut(s)	137
MvnI	CGCG	1 cut(s)	255
MwoI	GCNNNNNNNGC	2 cut(s)	226, 252
NciI	CCSGG	1 cut(s)	374
NdeII	GATC	7 cut(s)	10, 102, 113, 171, 241, 369, 402
NlaIII	CATG	2 cut(s)	214, 226
NlaIV	GGNNCC	4 cut(s)	104, 348, 371, 391
NmeAIII	GCCGAG	2 cut(s)	99, 187
PciSI	GCTCTTC	1 cut(s)	62
PfoI	TCCNGGA	1 cut(s)	372
PkrI	GCNGC	1 cut(s)	125
Ple19I	CGATCG	2 cut(s)	116, 244
PleI	GAGTC	1 cut(s)	275
PpsI	GAGTC	1 cut(s)	275
PpuMI	RGGWCCY	1 cut(s)	268
Psp124BI	GAGCTC	2 cut(s)	99, 312
Psp5II	RGGWCCY	1 cut(s)	268
Psp6I	CCWGG	1 cut(s)	135
PspGI	CCWGG	1 cut(s)	135
PspN4I	GGNNCC	4 cut(s)	104, 348, 371, 391
PspPI	GGNCC	3 cut(s)	139, 268, 390
PspPPI	RGGWCCY	1 cut(s)	268
PsuI	RGATCY	3 cut(s)	10, 102, 369
PvuI	CGATCG	2 cut(s)	116, 244
RgaI	GCGATCGC	1 cut(s)	116
SacI	GAGCTC	2 cut(s)	99, 312
SapI	GCTCTTC	1 cut(s)	62
SatI	GCNGC	1 cut(s)	124
Sau3AI	GATC	7 cut(s)	10, 102, 113, 171, 241, 369, 402
Sau96I	GGNCC	3 cut(s)	139, 268, 390
SchI	GAGTC	1 cut(s)	275
ScrFI	CCNGG	2 cut(s)	137, 374
SduI	GDGCHC	3 cut(s)	99, 237, 312
SetI	ASST	6 cut(s)	99, 210, 252, 270, 312, 381
SfaAI	GCGATCGC	1 cut(s)	116
SfaNI	GCATC	1 cut(s)	339
SgfI	GCGATCGC	1 cut(s)	116
SinI	GGWCC	2 cut(s)	268, 390
Sse9I	AATT	1 cut(s)	341
SsiI	CCGC	3 cut(s)	123, 177, 253
SstI	GAGCTC	2 cut(s)	99, 312
StyD4I	CCNGG	2 cut(s)	135, 372
TaqI	TCGA	7 cut(s)	35, 60, 186, 204, 240, 273, 360
TasI	AATT	1 cut(s)	341
TauI	GCSGC	1 cut(s)	126
TspDTI	ATGAA	3 cut(s)	53, 239, 409
TspGWI	ACGGA	2 cut(s)	78, 289
VpaK11BI	GGWCC	2 cut(s)	268, 390
XagI	CCTNNNNNAGG	1 cut(s)	275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.