RchiOBHm_Chr3g0455101

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002702
Physical Location & Seq
Reverse (-)
4963217 .. 4963465
249 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42207

Sequence Viewer

Length: 249 bp
ATGGGGATGGACATGAAGCCTAAGCCCTTCAATCGCCAGCTCTGCGATGCCTTCAAGGTCCTCGACAAGGACGCCACCGACTTCGTCTCCGTCTCGGAGCTCCGGCACATCCTCACCAGCATCGGCGAGAAATTGGAGGTCTTCCCTAATAATAACTTTGGGGCCGACGTCGTTGTTGGTGGCGCTAGAGACGAGTTTGTCGGTAGAAATGCGCCAGTGTCGTTGTTGGATGGCTTCTTGGGTGCCTAA

Protein Analysis

82

Amino Acids

8.93

Weight (kDa)

4.9

Isoelectric Point (pI)

25.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 14 - 42 8.2e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AatII GACGTC 1 cut(s) 171
AccB1I GGYRCC 1 cut(s) 242
AcyI GRCGYC 2 cut(s) 72, 168
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 2 cut(s) 31, 55
AluBI AGCT 2 cut(s) 40, 100
AluI AGCT 2 cut(s) 40, 100
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 3 cut(s) 91, 97, 183
AoxI GGCC 1 cut(s) 162
AspLEI GCGC 2 cut(s) 185, 214
AspS9I GGNCC 2 cut(s) 58, 162
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 1 cut(s) 58
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 102
BccI CCATC 1 cut(s) 224
BcoDI GTCTC 3 cut(s) 91, 97, 183
BfaI CTAG 1 cut(s) 186
BfoI RGCGCY 1 cut(s) 186
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 58, 162
BmiI GGNNCC 2 cut(s) 163, 244
BmsI GCATC 2 cut(s) 37, 129
BpiI GAAGAC 1 cut(s) 133
Bpu10I CCTNAGC 1 cut(s) 21
BsaHI GRCGYC 2 cut(s) 72, 168
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 215
BseGI GGATG 3 cut(s) 12, 108, 235
BseLI CCNNNNNNNGG 1 cut(s) 67
BseNI ACTGG 1 cut(s) 215
BshFI GGCC 1 cut(s) 164
BshNI GGYRCC 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 103
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 3 cut(s) 91, 97, 183
BsmBI CGTCTC 3 cut(s) 91, 97, 183
BsnI GGCC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 102
BspANI GGCC 1 cut(s) 164
BspLI GGNNCC 2 cut(s) 163, 244
BspT107I GGYRCC 1 cut(s) 242
BsrI ACTGG 1 cut(s) 215
BssNI GRCGYC 2 cut(s) 72, 168
BstACI GRCGYC 2 cut(s) 72, 168
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 1 cut(s) 21
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 3 cut(s) 12, 108, 235
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 2 cut(s) 185, 214
BstMAI GTCTC 3 cut(s) 91, 97, 183
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstV2I GAAGAC 1 cut(s) 133
BsuRI GGCC 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 3 cut(s) 12, 108, 235
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 38
CfoI GCGC 2 cut(s) 185, 214
Cfr13I GGNCC 2 cut(s) 58, 162
CseI GACGC 1 cut(s) 80
CviAII CATG 1 cut(s) 13
CviJI RGCY 6 cut(s) 19, 25, 40, 100, 164, 234
CviKI_1 RGCY 6 cut(s) 19, 25, 40, 100, 164, 234
DdeI CTNAG 1 cut(s) 21
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
Ecl136II GAGCTC 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 58
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 58
EcoT38I GRGCYC 1 cut(s) 102
Esp3I CGTCTC 3 cut(s) 91, 97, 183
FaeI CATG 1 cut(s) 16
FaiI YATR 1 cut(s) 14
FatI CATG 1 cut(s) 12
FokI GGATG 3 cut(s) 19, 95, 242
FriOI GRGCYC 1 cut(s) 102
FspBI CTAG 1 cut(s) 186
GlaI GCGC 2 cut(s) 184, 213
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 1 cut(s) 164
HapII CCGG 1 cut(s) 103
HgaI GACGC 1 cut(s) 80
HhaI GCGC 2 cut(s) 185, 214
Hin1I GRCGYC 2 cut(s) 72, 168
Hin1II CATG 1 cut(s) 16
Hin6I GCGC 2 cut(s) 183, 212
HinP1I GCGC 2 cut(s) 183, 212
HpaII CCGG 1 cut(s) 103
HphI GGTGA 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 97
Hpy99I CGWCG 2 cut(s) 170, 173
HpyAV CCTTC 2 cut(s) 37, 61
HpyCH4IV ACGT 1 cut(s) 168
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpyF3I CTNAG 1 cut(s) 21
HpySE526I ACGT 1 cut(s) 168
Hsp92I GRCGYC 2 cut(s) 72, 168
Hsp92II CATG 1 cut(s) 16
HspAI GCGC 2 cut(s) 183, 212
LmnI GCTCC 2 cut(s) 97, 105
LpnPI CCDG 4 cut(s) 50, 116, 130, 228
LweI GCATC 2 cut(s) 37, 129
MaeI CTAG 1 cut(s) 186
MaeII ACGT 1 cut(s) 168
MboII GAAGA 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 3 cut(s) 71, 122, 130
MspI CCGG 1 cut(s) 103
MwoI GCNNNNNNNGC 1 cut(s) 42
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 2 cut(s) 163, 244
PflFI GACNNNGTC 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 163, 244
PspPI GGNCC 2 cut(s) 58, 162
PspPPI RGGWCCY 1 cut(s) 58
PsyI GACNNNGTC 1 cut(s) 83
SacI GAGCTC 1 cut(s) 102
Sau96I GGNCC 2 cut(s) 58, 162
SduI GDGCHC 1 cut(s) 102
SetI ASST 5 cut(s) 42, 60, 102, 141, 171
SfaNI GCATC 2 cut(s) 37, 129
SinI GGWCC 1 cut(s) 58
Sse9I AATT 1 cut(s) 131
SspMI CTAG 1 cut(s) 186
SstI GAGCTC 1 cut(s) 102
TaiI ACGT 1 cut(s) 171
TaqI TCGA 1 cut(s) 63
TasI AATT 1 cut(s) 131
TscAI CASTG 1 cut(s) 222
TspDTI ATGAA 1 cut(s) 29
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 222
Tth111I GACNNNGTC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 58
XagI CCTNNNNNAGG 1 cut(s) 65
XspI CTAG 1 cut(s) 186
ZraI GACGTC 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.