Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002702

Physical Location & Seq

Genomic Coordinates Forward (+)

16991449 .. 16992021

Gene Structure 573 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1151100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 573 bp

ATGCCTAATGGGCCCTCTCGCCCTATTTTTTTTCACTGTCTGAAGTCACACATTTACACCAACACAAGCGCAGAAACACCATCCAGAGAGGAGAGAGACCCGAAAACCCGAATCGCGAACCCGGAAAAAATGGGCAAAGATCTGAGCGATGACCAAGTATCGTCGATGAAGGAGGCGTTCACGCTCTTCGACACCGACAACGACGGCAAGATTGCTCCGTCGGAGCTCGGGATCCTAATGCGATCGCTCGGCGACAACCCGACCCAGGCCCAACTCAAATCCATAGTCGTCGAGGAGAAGCTCACCGCCCCCTTCGACTTCCCTCGCTTCCTCGACCTTATGGCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTCCGCGACGCCTTCAAGGTCCTCGACAAGGACTCAACCGGCTTCGTGTCCGTGTCGGAGCTCCGGCACATCCTCACCAGCATCGGCGAGAAATTGGAGCCGTCCGAGTTCGACGAGTGGATCCAGGAGGTGGATGTCGGGTCTGATGGCAAGATCAAATACGAGGACTTCATTGCCAGAACGGTGGCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko03010 ko04626 map03010 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_6 EF-hand_7

Protein Analysis

191

Amino Acids

21.52

Weight (kDa)

5.09

Isoelectric Point (pI)

40.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	55 - 96	3.6e-06	EF-hand domain pair
EF-hand_6	PF13405	56 - 84	8e-07	EF-hand domain
EF-hand_1	PF00036	56 - 83	4.8e-06	EF hand domain
EF-hand_7	PF13499	125 - 185	8.8e-16	EF-hand domain pair
EF-hand_6	PF13405	126 - 155	8.4e-08	EF-hand domain
EF-hand_1	PF00036	127 - 153	9.1e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002702)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12310 AT1G62820
fragaria_vesca	FvH4_5g00650 FvH4_7g20700
malus_domestica	MD03G1151100.v1.1 MD04G1079800.v1.1 MD06G1074600.v1.1 MD07G1265800.v1.1 MD11G1152700.v1.1 MD17G1283300.v1.1
prunus_persica	Prupe.5G079300_v2.0.a1
pyrus_communis	pycom06g05340
rosa_chinensis	RchiOBHm_Chr1g0364931 RchiOBHm_Chr3g0455101 RchiOBHm_Chr5g0045151 RchiOBHm_Chr6g0299731 RchiOBHm_Chr7g0201611
rosa_laevigata	RLG00000027458
rosa_multiflora	Rmu_sc0002502.1_g000006 Rmu_sc0002895.1_g000017 Rmu_sc0006410.1_g000028 Rmu_sc0007033.1_g000001 Rmu_sc0011159.1_g000005 Rmu_ssc0000112.1_g000026 Rmu_ssc0000304.1_g000001
rosa_roxburghii	Rroxscaffold_3G00255200 Rroxscaffold_4G00292060
rosa_rugosa	Rorug01G0318600 Rorug07G0065200
rosa_samantha	Rh1BG288500 Rh1CG305200 Rh1DG320100 Rh2CG069200 Rh7AG191700 Rh7BG194000 Rh7DG197000
rosa_wichuraiana	Rw1G029000 Rw5G028070 Rw7G016820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	508
AccII	CGCG	2 cut(s)	116, 384
AciI	CCGC	2 cut(s)	306, 382
AclWI	GGATC	4 cut(s)	226, 239, 493, 506
AcoI	YGGCCR	2 cut(s)	342, 564
AcuI	CTGAAG	1 cut(s)	62
AcyI	GRCGYC	1 cut(s)	387
AfiI	CCNNNNNNNGG	3 cut(s)	265, 406, 508
AgsI	TTSAA	1 cut(s)	394
AjnI	CCWGG	2 cut(s)	264, 501
AluBI	AGCT	4 cut(s)	226, 301, 379, 439
AluI	AGCT	4 cut(s)	226, 301, 379, 439
Alw21I	GWGCWC	2 cut(s)	228, 441
Alw26I	GTCTC	1 cut(s)	90
AlwI	GGATC	4 cut(s)	226, 239, 493, 506
Ama87I	CYCGRG	2 cut(s)	227, 359
AoxI	GGCC	4 cut(s)	11, 267, 342, 564
ApaI	GGGCCC	1 cut(s)	15
ArsI	GACNNNNNNTTYG	2 cut(s)	270, 302
AsiSI	GCGATCGC	1 cut(s)	245
AspLEI	GCGC	1 cut(s)	71
AspS9I	GGNCC	4 cut(s)	11, 12, 268, 397
AsuC2I	CCSGG	1 cut(s)	122
AsuHPI	GGTGA	2 cut(s)	295, 445
AvaI	CYCGRG	2 cut(s)	227, 359
AvaII	GGWCC	1 cut(s)	397
BaeGI	GKGCMC	1 cut(s)	15
BalI	TGGCCA	2 cut(s)	344, 566
BamHI	GGATCC	2 cut(s)	231, 498
BanII	GRGCYC	4 cut(s)	15, 228, 366, 441
Bbv12I	GWGCWC	2 cut(s)	228, 441
BccI	CCATC	2 cut(s)	88, 518
BceAI	ACGGC	2 cut(s)	220, 463
BciT130I	CCWGG	2 cut(s)	266, 503
BcnI	CCSGG	1 cut(s)	122
BcoDI	GTCTC	1 cut(s)	90
BglI	GCCNNNNNGGC	1 cut(s)	10
BglII	AGATCT	1 cut(s)	139
Bme1390I	CCNGG	3 cut(s)	122, 266, 503
Bme18I	GGWCC	1 cut(s)	397
BmeT110I	CYCGRG	2 cut(s)	227, 359
BmgT120I	GGNCC	4 cut(s)	11, 12, 268, 397
BmiI	GGNNCC	4 cut(s)	13, 233, 477, 500
BmrFI	CCNGG	3 cut(s)	122, 266, 503
BmsI	GCATC	1 cut(s)	468
BpuMI	CCSGG	1 cut(s)	122
BsaHI	GRCGYC	1 cut(s)	387
BsaI	GGTCTC	1 cut(s)	90
BsaJI	CCNNGG	1 cut(s)	264
Bsc4I	CCNNNNNNNGG	3 cut(s)	265, 406, 508
Bse118I	RCCGGY	1 cut(s)	416
Bse3DI	GCAATG	1 cut(s)	549
BseBI	CCWGG	2 cut(s)	266, 503
BseDI	CCNNGG	1 cut(s)	264
BseGI	GGATG	3 cut(s)	80, 447, 517
BseLI	CCNNNNNNNGG	3 cut(s)	265, 406, 508
BseMI	GCAATG	1 cut(s)	549
BseMII	CTCAG	1 cut(s)	134
BseRI	GAGGAG	2 cut(s)	104, 308
BseSI	GKGCMC	1 cut(s)	15
Bsh1236I	CGCG	2 cut(s)	116, 384
Bsh1285I	CGRYCG	2 cut(s)	245, 373
BshFI	GGCC	4 cut(s)	13, 269, 344, 566
BsiEI	CGRYCG	2 cut(s)	245, 373
BsiHKAI	GWGCWC	2 cut(s)	228, 441
BsiHKCI	CYCGRG	2 cut(s)	227, 359
BsiSI	CCGG	3 cut(s)	122, 417, 442
BslI	CCNNNNNNNGG	3 cut(s)	265, 406, 508
BsmAI	GTCTC	1 cut(s)	90
BsnI	GGCC	4 cut(s)	13, 269, 344, 566
Bso31I	GGTCTC	1 cut(s)	90
BsoBI	CYCGRG	2 cut(s)	227, 359
Bsp120I	GGGCCC	1 cut(s)	11
Bsp1286I	GDGCHC	4 cut(s)	15, 228, 366, 441
Bsp143I	GATC	6 cut(s)	139, 231, 242, 370, 498, 531
Bsp68I	TCGCGA	1 cut(s)	116
BspACI	CCGC	2 cut(s)	306, 382
BspANI	GGCC	4 cut(s)	13, 269, 344, 566
BspCNI	CTCAG	1 cut(s)	135
BspFNI	CGCG	2 cut(s)	116, 384
BspLI	GGNNCC	4 cut(s)	13, 233, 477, 500
BspPI	GGATC	4 cut(s)	226, 239, 493, 506
BspQI	GCTCTTC	1 cut(s)	191
BspTNI	GGTCTC	1 cut(s)	90
BsrDI	GCAATG	1 cut(s)	549
BsrFI	RCCGGY	1 cut(s)	416
BssAI	RCCGGY	1 cut(s)	416
BssECI	CCNNGG	1 cut(s)	264
BssMI	GATC	6 cut(s)	139, 231, 242, 370, 498, 531
BssNI	GRCGYC	1 cut(s)	387
Bst2UI	CCWGG	2 cut(s)	266, 503
Bst4CI	ACNGT	2 cut(s)	38, 562
Bst6I	CTCTTC	1 cut(s)	191
BstACI	GRCGYC	1 cut(s)	387
BstC8I	GCNNGC	1 cut(s)	377
BstDEI	CTNAG	1 cut(s)	143
BstENI	CCTNNNNNAGG	1 cut(s)	404
BstF5I	GGATG	3 cut(s)	80, 447, 517
BstFNI	CGCG	2 cut(s)	116, 384
BstHHI	GCGC	1 cut(s)	71
BstKTI	GATC	6 cut(s)	142, 234, 245, 373, 501, 534
BstMAI	GTCTC	1 cut(s)	90
BstMBI	GATC	6 cut(s)	139, 231, 242, 370, 498, 531
BstMCI	CGRYCG	2 cut(s)	245, 373
BstMWI	GCNNNNNNNGC	3 cut(s)	10, 355, 381
BstNI	CCWGG	2 cut(s)	266, 503
BstSCI	CCNGG	3 cut(s)	120, 264, 501
BstSLI	GKGCMC	1 cut(s)	15
BstUI	CGCG	2 cut(s)	116, 384
BstX2I	RGATCY	3 cut(s)	139, 231, 498
BstXI	CCANNNNNNTGG	1 cut(s)	562
BstYI	RGATCY	3 cut(s)	139, 231, 498
BsuRI	GGCC	4 cut(s)	13, 269, 344, 566
BtgZI	GCGATG	1 cut(s)	162
BtsCI	GGATG	3 cut(s)	80, 447, 517
BtsIMutI	CAGTG	1 cut(s)	34
BtuMI	TCGCGA	1 cut(s)	116
Cac8I	GCNNGC	1 cut(s)	377
CfoI	GCGC	1 cut(s)	71
Cfr10I	RCCGGY	1 cut(s)	416
Cfr13I	GGNCC	4 cut(s)	11, 12, 268, 397
CseI	GACGC	1 cut(s)	395
CviAII	CATG	1 cut(s)	352
DdeI	CTNAG	1 cut(s)	143
DpnI	GATC	6 cut(s)	141, 233, 244, 372, 500, 533
DpnII	GATC	6 cut(s)	139, 231, 242, 370, 498, 531
EaeI	YGGCCR	2 cut(s)	342, 564
Eam1104I	CTCTTC	1 cut(s)	191
EarI	CTCTTC	1 cut(s)	191
Ecl136II	GAGCTC	2 cut(s)	226, 439
Eco24I	GRGCYC	4 cut(s)	15, 228, 366, 441
Eco31I	GGTCTC	1 cut(s)	90
Eco47I	GGWCC	1 cut(s)	397
Eco53kI	GAGCTC	2 cut(s)	226, 439
Eco57I	CTGAAG	1 cut(s)	62
Eco88I	CYCGRG	2 cut(s)	227, 359
EcoICRI	GAGCTC	2 cut(s)	226, 439
EcoNI	CCTNNNNNAGG	1 cut(s)	404
EcoO109I	RGGNCCY	2 cut(s)	12, 397
EcoRII	CCWGG	2 cut(s)	264, 501
EcoT38I	GRGCYC	4 cut(s)	15, 228, 366, 441
FaeI	CATG	1 cut(s)	355
FaiI	YATR	3 cut(s)	284, 341, 353
FatI	CATG	1 cut(s)	351
FokI	GGATG	3 cut(s)	67, 434, 524
FriOI	GRGCYC	4 cut(s)	15, 228, 366, 441
GlaI	GCGC	1 cut(s)	70
HaeIII	GGCC	4 cut(s)	13, 269, 344, 566
HapII	CCGG	3 cut(s)	122, 417, 442
HgaI	GACGC	1 cut(s)	395
HhaI	GCGC	1 cut(s)	71
Hin1I	GRCGYC	1 cut(s)	387
Hin1II	CATG	1 cut(s)	355
Hin6I	GCGC	1 cut(s)	69
HinP1I	GCGC	1 cut(s)	69
HinfI	GANTC	2 cut(s)	111, 410
HpaII	CCGG	3 cut(s)	122, 417, 442
HphI	GGTGA	2 cut(s)	295, 445
Hpy166II	GTNNAC	1 cut(s)	180
Hpy188I	TCNGA	6 cut(s)	42, 144, 223, 436, 484, 523
Hpy188III	TCNNGA	3 cut(s)	84, 115, 229
Hpy8I	GTNNAC	1 cut(s)	180
Hpy99I	CGWCG	6 cut(s)	166, 206, 223, 293, 389, 494
HpyAV	CCTTC	4 cut(s)	163, 322, 376, 400
HpyCH4III	ACNGT	2 cut(s)	38, 562
HpyF10VI	GCNNNNNNNGC	3 cut(s)	10, 355, 381
HpyF3I	CTNAG	1 cut(s)	143
Hsp92I	GRCGYC	1 cut(s)	387
Hsp92II	CATG	1 cut(s)	355
HspAI	GCGC	1 cut(s)	69
Kzo9I	GATC	6 cut(s)	139, 231, 242, 370, 498, 531
LguI	GCTCTTC	1 cut(s)	191
LmnI	GCTCC	6 cut(s)	220, 223, 384, 436, 444, 475
LweI	GCATC	1 cut(s)	468
MaeIII	GTNAC	1 cut(s)	45
MalI	GATC	6 cut(s)	141, 233, 244, 372, 500, 533
MboI	GATC	6 cut(s)	139, 231, 242, 370, 498, 531
MboII	GAAGA	1 cut(s)	178
MflI	RGATCY	3 cut(s)	139, 231, 498
MhlI	GDGCHC	4 cut(s)	15, 228, 366, 441
MlsI	TGGCCA	2 cut(s)	344, 566
MluCI	AATT	1 cut(s)	470
MluNI	TGGCCA	2 cut(s)	344, 566
MlyI	GAGTC	1 cut(s)	404
MmeI	TCCRAC	2 cut(s)	201, 414
Mox20I	TGGCCA	2 cut(s)	344, 566
MscI	TGGCCA	2 cut(s)	344, 566
Msp20I	TGGCCA	2 cut(s)	344, 566
MspI	CCGG	3 cut(s)	122, 417, 442
MspR9I	CCNGG	3 cut(s)	122, 266, 503
MvaI	CCWGG	2 cut(s)	266, 503
MvnI	CGCG	2 cut(s)	116, 384
MwoI	GCNNNNNNNGC	3 cut(s)	10, 355, 381
NciI	CCSGG	1 cut(s)	122
NdeII	GATC	6 cut(s)	139, 231, 242, 370, 498, 531
NlaIII	CATG	1 cut(s)	355
NlaIV	GGNNCC	4 cut(s)	13, 233, 477, 500
NmeAIII	GCCGAG	1 cut(s)	228
NmuCI	GTSAC	1 cut(s)	45
NruI	TCGCGA	1 cut(s)	116
PciSI	GCTCTTC	1 cut(s)	191
PfeI	GAWTC	1 cut(s)	111
PflMI	CCANNNNNTGG	1 cut(s)	508
PfoI	TCCNGGA	1 cut(s)	501
Ple19I	CGATCG	2 cut(s)	245, 373
PleI	GAGTC	1 cut(s)	404
PpsI	GAGTC	1 cut(s)	404
PpuMI	RGGWCCY	1 cut(s)	397
Psp124BI	GAGCTC	2 cut(s)	228, 441
Psp5II	RGGWCCY	1 cut(s)	397
Psp6I	CCWGG	2 cut(s)	264, 501
PspGI	CCWGG	2 cut(s)	264, 501
PspN4I	GGNNCC	4 cut(s)	13, 233, 477, 500
PspOMI	GGGCCC	1 cut(s)	11
PspPI	GGNCC	4 cut(s)	11, 12, 268, 397
PspPPI	RGGWCCY	1 cut(s)	397
PsuI	RGATCY	3 cut(s)	139, 231, 498
PvuI	CGATCG	2 cut(s)	245, 373
RgaI	GCGATCGC	1 cut(s)	245
RruI	TCGCGA	1 cut(s)	116
SacI	GAGCTC	2 cut(s)	228, 441
SapI	GCTCTTC	1 cut(s)	191
Sau3AI	GATC	6 cut(s)	139, 231, 242, 370, 498, 531
Sau96I	GGNCC	4 cut(s)	11, 12, 268, 397
SchI	GAGTC	1 cut(s)	404
ScrFI	CCNGG	3 cut(s)	122, 266, 503
SduI	GDGCHC	4 cut(s)	15, 228, 366, 441
SetI	ASST	7 cut(s)	228, 303, 339, 381, 399, 441, 510
SfaAI	GCGATCGC	1 cut(s)	245
SfaNI	GCATC	1 cut(s)	468
SgfI	GCGATCGC	1 cut(s)	245
SinI	GGWCC	1 cut(s)	397
Sse9I	AATT	1 cut(s)	470
SsiI	CCGC	2 cut(s)	306, 382
SstI	GAGCTC	2 cut(s)	228, 441
StyD4I	CCNGG	3 cut(s)	120, 264, 501
TaaI	ACNGT	2 cut(s)	38, 562
TaqI	TCGA	8 cut(s)	164, 189, 291, 315, 333, 369, 402, 489
TasI	AATT	1 cut(s)	470
TfiI	GAWTC	1 cut(s)	111
TscAI	CASTG	1 cut(s)	41
TseFI	GTSAC	1 cut(s)	45
Tsp45I	GTSAC	1 cut(s)	45
TspDTI	ATGAA	3 cut(s)	182, 368, 538
TspGWI	ACGGA	2 cut(s)	207, 418
TspRI	CASTG	1 cut(s)	41
Van91I	CCANNNNNTGG	1 cut(s)	508
VpaK11BI	GGWCC	1 cut(s)	397
XagI	CCTNNNNNAGG	1 cut(s)	404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.