MD06G1074600.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002702
Physical Location & Seq
Forward (+)
18630173 .. 18630616
444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1074600.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGGCAAGGATTTGAGCGATGACCAAGTATCGTCGATGAAGGAGGCGTTCATGCTCTTCGATACCGACAACGATGGCAAGATTGCTCCGTCCGAGCTAGGGATCCTAATGCGATCGCTCGGCGGCAACCCGACCCAGGCCCAACTCAAATCCATAGTCGCCGAGGAGAAGCTCACCGCCCCCTTCAACTTCCCTCGCTTCCTCGAACTCATGGCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTCCGTGACTCCTTCAAGGTTCTCGACAAGGACTCCACCGGGTTCGTCTCCGTCTCGGAGCTCCGGCACATCCTCACCAACATCGGCGAGAAATTGGAGCCCTCCGAATTTGACGAGTGGATCCGAGAGGTGGATGTCGGGTCCGATGGGAAGATCAAATACGAGGACTTCATTGCCAGAATGGTGGCCAAGTGA

Protein Analysis

148

Amino Acids

16.64

Weight (kDa)

4.9

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 12 - 54 2.3e-06 EF-hand domain pair
EF-hand_1 PF00036 13 - 40 1.7e-06 EF hand domain
EF-hand_6 PF13405 13 - 41 3.2e-07 EF-hand domain
EF-hand_7 PF13499 82 - 144 6e-16 EF-hand domain pair
EF-hand_6 PF13405 83 - 112 7.8e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 123, 177
AclWI GGATC 4 cut(s) 97, 110, 364, 377
AcoI YGGCCR 2 cut(s) 213, 435
AcsI RAATTY 1 cut(s) 356
AfiI CCNNNNNNNGG 3 cut(s) 99, 136, 379
AgsI TTSAA 2 cut(s) 187, 265
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 4 cut(s) 97, 172, 250, 310
AluI AGCT 4 cut(s) 97, 172, 250, 310
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 2 cut(s) 301, 307
AlwI GGATC 4 cut(s) 97, 110, 364, 377
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 3 cut(s) 138, 213, 435
ApoI RAATTY 1 cut(s) 356
ArsI GACNNNNNNTTYG 2 cut(s) 141, 173
AsiSI GCGATCGC 1 cut(s) 116
AspS9I GGNCC 2 cut(s) 139, 390
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 2 cut(s) 166, 316
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 1 cut(s) 390
BalI TGGCCA 2 cut(s) 215, 437
BamHI GGATCC 2 cut(s) 102, 369
BanII GRGCYC 3 cut(s) 237, 312, 351
BarI GAAGNNNNNNTAC 2 cut(s) 392, 424
Bbv12I GWGCWC 1 cut(s) 312
BccI CCATC 2 cut(s) 68, 389
BciT130I CCWGG 1 cut(s) 137
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 301, 307
BfaI CTAG 1 cut(s) 98
BisI GCNGC 1 cut(s) 124
BlsI GCNGC 1 cut(s) 125
Bme1390I CCNGG 2 cut(s) 137, 289
Bme18I GGWCC 1 cut(s) 390
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 2 cut(s) 139, 390
BmiI GGNNCC 4 cut(s) 104, 348, 371, 391
BmrFI CCNGG 2 cut(s) 137, 289
BpuMI CCSGG 1 cut(s) 289
BsaJI CCNNGG 2 cut(s) 135, 162
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 136, 379
Bse3DI GCAATG 1 cut(s) 420
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 135, 162
BseGI GGATG 2 cut(s) 318, 388
BseLI CCNNNNNNNGG 3 cut(s) 99, 136, 379
BseMI GCAATG 1 cut(s) 420
BseRI GAGGAG 1 cut(s) 179
Bsh1285I CGRYCG 2 cut(s) 116, 244
BshFI GGCC 3 cut(s) 140, 215, 437
BsiEI CGRYCG 2 cut(s) 116, 244
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 2 cut(s) 288, 313
BslI CCNNNNNNNGG 3 cut(s) 99, 136, 379
BsmAI GTCTC 2 cut(s) 301, 307
BsmBI CGTCTC 2 cut(s) 301, 307
BsnI GGCC 3 cut(s) 140, 215, 437
BsoBI CYCGRG 1 cut(s) 230
Bsp1286I GDGCHC 3 cut(s) 237, 312, 351
Bsp143I GATC 5 cut(s) 102, 113, 241, 369, 402
BspACI CCGC 2 cut(s) 123, 177
BspANI GGCC 3 cut(s) 140, 215, 437
BspLI GGNNCC 4 cut(s) 104, 348, 371, 391
BspPI GGATC 4 cut(s) 97, 110, 364, 377
BspQI GCTCTTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 420
BssECI CCNNGG 2 cut(s) 135, 162
BssMI GATC 5 cut(s) 102, 113, 241, 369, 402
Bst2UI CCWGG 1 cut(s) 137
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 248
BstF5I GGATG 2 cut(s) 318, 388
BstKTI GATC 5 cut(s) 105, 116, 244, 372, 405
BstMAI GTCTC 2 cut(s) 301, 307
BstMBI GATC 5 cut(s) 102, 113, 241, 369, 402
BstMCI CGRYCG 2 cut(s) 116, 244
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 2 cut(s) 135, 287
BstX2I RGATCY 2 cut(s) 102, 369
BstXI CCANNNNNNTGG 1 cut(s) 433
BstYI RGATCY 2 cut(s) 102, 369
BsuRI GGCC 3 cut(s) 140, 215, 437
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 2 cut(s) 318, 388
Cac8I GCNNGC 1 cut(s) 248
Cfr13I GGNCC 2 cut(s) 139, 390
CviAII CATG 3 cut(s) 52, 211, 223
DpnI GATC 5 cut(s) 104, 115, 243, 371, 404
DpnII GATC 5 cut(s) 102, 113, 241, 369, 402
EaeI YGGCCR 2 cut(s) 213, 435
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 310
Eco24I GRGCYC 3 cut(s) 237, 312, 351
Eco47I GGWCC 1 cut(s) 390
Eco53kI GAGCTC 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 135
EcoT38I GRGCYC 3 cut(s) 237, 312, 351
Esp3I CGTCTC 2 cut(s) 301, 307
FaeI CATG 3 cut(s) 55, 214, 226
FaiI YATR 4 cut(s) 53, 155, 212, 224
FatI CATG 3 cut(s) 51, 210, 222
Fnu4HI GCNGC 1 cut(s) 124
FokI GGATG 2 cut(s) 305, 395
FriOI GRGCYC 3 cut(s) 237, 312, 351
Fsp4HI GCNGC 1 cut(s) 124
FspBI CTAG 1 cut(s) 98
GluI GCNGC 1 cut(s) 124
HaeIII GGCC 3 cut(s) 140, 215, 437
HapII CCGG 2 cut(s) 288, 313
Hin1II CATG 3 cut(s) 55, 214, 226
HinfI GANTC 2 cut(s) 257, 281
HpaII CCGG 2 cut(s) 288, 313
HphI GGTGA 2 cut(s) 166, 316
Hpy188I TCNGA 5 cut(s) 94, 307, 355, 374, 394
Hpy188III TCNNGA 1 cut(s) 272
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 4 cut(s) 34, 193, 247, 271
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
Hsp92II CATG 3 cut(s) 55, 214, 226
Kzo9I GATC 5 cut(s) 102, 113, 241, 369, 402
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 5 cut(s) 91, 255, 307, 315, 346
LpnPI CCDG 6 cut(s) 122, 149, 260, 301, 326, 439
MaeI CTAG 1 cut(s) 98
MaeIII GTNAC 1 cut(s) 254
MalI GATC 5 cut(s) 104, 115, 243, 371, 404
MboI GATC 5 cut(s) 102, 113, 241, 369, 402
MboII GAAGA 2 cut(s) 49, 412
MflI RGATCY 2 cut(s) 102, 369
MhlI GDGCHC 3 cut(s) 237, 312, 351
MlsI TGGCCA 2 cut(s) 215, 437
MluCI AATT 2 cut(s) 341, 356
MluNI TGGCCA 2 cut(s) 215, 437
MlyI GAGTC 2 cut(s) 251, 275
MnlI CCTC 8 cut(s) 37, 157, 204, 212, 332, 361, 370, 406
Mox20I TGGCCA 2 cut(s) 215, 437
MscI TGGCCA 2 cut(s) 215, 437
Msp20I TGGCCA 2 cut(s) 215, 437
MspI CCGG 2 cut(s) 288, 313
MspR9I CCNGG 2 cut(s) 137, 289
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 226
NciI CCSGG 1 cut(s) 289
NdeII GATC 5 cut(s) 102, 113, 241, 369, 402
NlaIII CATG 3 cut(s) 55, 214, 226
NlaIV GGNNCC 4 cut(s) 104, 348, 371, 391
NmeAIII GCCGAG 2 cut(s) 99, 187
NmuCI GTSAC 1 cut(s) 254
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 250
PkrI GCNGC 1 cut(s) 125
Ple19I CGATCG 2 cut(s) 116, 244
PleI GAGTC 2 cut(s) 251, 275
PpsI GAGTC 2 cut(s) 251, 275
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 4 cut(s) 104, 348, 371, 391
PspPI GGNCC 2 cut(s) 139, 390
PsuI RGATCY 2 cut(s) 102, 369
PvuI CGATCG 2 cut(s) 116, 244
RgaI GCGATCGC 1 cut(s) 116
SacI GAGCTC 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 1 cut(s) 124
Sau3AI GATC 5 cut(s) 102, 113, 241, 369, 402
Sau96I GGNCC 2 cut(s) 139, 390
SchI GAGTC 2 cut(s) 251, 275
ScrFI CCNGG 2 cut(s) 137, 289
SduI GDGCHC 3 cut(s) 237, 312, 351
SetI ASST 6 cut(s) 99, 174, 252, 270, 312, 381
SfaAI GCGATCGC 1 cut(s) 116
SgfI GCGATCGC 1 cut(s) 116
SinI GGWCC 1 cut(s) 390
Sse9I AATT 2 cut(s) 341, 356
SsiI CCGC 2 cut(s) 123, 177
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 2 cut(s) 135, 287
TaqI TCGA 5 cut(s) 35, 60, 204, 240, 273
TasI AATT 2 cut(s) 341, 356
TauI GCSGC 1 cut(s) 126
TseFI GTSAC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 4 cut(s) 40, 53, 239, 409
TspGWI ACGGA 3 cut(s) 78, 242, 289
VpaK11BI GGWCC 1 cut(s) 390
XapI RAATTY 1 cut(s) 356
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.