MD04G1079900.v1.1

Maspardin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012477
Physical Location & Seq
Reverse (-)
10974874 .. 10980533
5660 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1079900.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGAAAGGCGTCTACTCGGCGCCCGGAGATTACATCTACTTCAAGTCCCAGGTCCCTCTTCACAAGATCCCTATTGGCACAAAGCAGTGGCGATACTATGATTTTGGCCCAAAAGTGGTACCTCCTCTTATTTGTCTTCCTGGAACAGCAGGGACAGCCGATGTCTGCTACAAGCAGATCATGTCATTGTCCATGAAGGGTTATCGGGTGATTTCTGTTGATATTCCACGTGTATGGAATCATCATGAGTGGATTCTAGCATTCGAGAAATTCTTGGATGCTATTGATGTTCATCACATACATATCTATGGTACATCCCTTGGGGGCTTCTTGGCTCAACTTTTTGCTCAGCATCGTCCAAGACGAGTTCGATCATTGATACTATCAAACACATTTTTGGACACGCGCAGCTTCTCTGCAGCAATGCCTTGGGCTCCCGTCATTAGTTGGACCCCTTCTTTTTTGTTGAAAAGATATGTCTTGACCGGAATACATGATGGCCCCCATGAGCCATTTATAGCAGATTCAGTGGACTTCGTTGTTTCTCAGGTTGAGACGCTCTCAAGAGAGGATCTGGCCTCCAGGTTGACTTTAACAACTGATGCTGCTTCAGTTGGACCTCTGTTGCTTACCGATTCATTCATCACTATAATGGATACAAATGACTACAGTGGGATTCCTCAACAACTCAAAGATGAAGTTGGTGAAAGGTATCCTGACGCAAGGCGAGCACAGTTGAAGAGTGGGGGAGATTTTCCATTCCTGTCCCGACCGGATGAAGTCAACTTACATCTTCAGCTGCATCTAAGACGTGTTGGTGTGGAACCTCGACCTGACTTGGTTCCAGGTTTTTCAAAGGGAGGTGGTGGTAGTTCTAGCAAAGAGAATGAGAAAAAAGACGAGAAAAAAGATCCAGAAGATCCACCCAAGGATGATAGGGGAAACCCGGATAATCCATTTGCAGAAACTCGGCTACCTCCTGCTCCAGAAAGTTCAGAATCTCGTAGCTCAGACAACCAGCTTGTCAGCAACGCAAAATTTTGCCACGTCGGTAATGAAGACATGATGGTTTCCTTGCCTTCTGGATTCTTGAATAACCAACATGCTATAGCCACTGAATTCGTTATGCGACTTAGTTGGGAAATATTCGCTCTTCATCTGCATTCATATCATGTGGTATCGTTGTACATAAGTCTGAACTGTAATCGGAAGCTCAGGCCTCTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

410

Amino Acids

45.96

Weight (kDa)

6.6

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 41 - 160 6.8e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 43 - 261 1.4e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12230
fragaria_vesca FvH4_5g00640 FvH4_5g00640 FvH4_5g00640
malus_domestica MD04G1079900.v1.1 MD06G1074900.v1.1
prunus_persica Prupe.5G079200_v2.0.a1
pyrus_communis pycom04g07200 pycom06g05350
rosa_chinensis RchiOBHm_Chr7g0201621
rosa_laevigata RLG00000003661
rosa_multiflora Rmu_sc0004811.1_g000001
rosa_roxburghii Rroxscaffold_3G00255190
rosa_rugosa Rorug07G0065200
rosa_samantha Rh7AG191900 Rh7BG194100 Rh7CG203100 Rh7DG197100
rosa_wichuraiana Rw7G016830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 118
AccB1I GGYRCC 2 cut(s) 19, 118
AccI GTMKAC 1 cut(s) 12
AccII CGCG 1 cut(s) 406
AclWI GGATC 4 cut(s) 61, 579, 905, 914
AcsI RAATTY 3 cut(s) 269, 1037, 1118
AcuI CTGAAG 2 cut(s) 594, 779
AcvI CACGTG 1 cut(s) 230
AcyI GRCGYC 2 cut(s) 9, 20
AfaI GTAC 3 cut(s) 120, 313, 1187
AfiI CCNNNNNNNGG 1 cut(s) 115
AflIII ACRYGT 2 cut(s) 229, 811
AgsI TTSAA 5 cut(s) 43, 469, 739, 855, 1093
AjiI CACGTC 2 cut(s) 812, 1048
AjnI CCWGG 4 cut(s) 48, 139, 581, 844
AluBI AGCT 5 cut(s) 411, 799, 1008, 1021, 1213
AluI AGCT 5 cut(s) 411, 799, 1008, 1021, 1213
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 4 cut(s) 61, 579, 905, 914
AoxI GGCC 4 cut(s) 106, 499, 576, 1217
ApeKI GCWGC 4 cut(s) 408, 419, 605, 799
ApoI RAATTY 3 cut(s) 269, 1037, 1118
Asp718I GGTACC 1 cut(s) 118
AspLEI GCGC 2 cut(s) 22, 408
AspS9I GGNCC 5 cut(s) 52, 107, 450, 500, 617
AsuC2I CCSGG 2 cut(s) 24, 947
AsuHPI GGTGA 2 cut(s) 220, 716
AvaII GGWCC 3 cut(s) 52, 450, 617
BanI GGYRCC 2 cut(s) 19, 118
BanII GRGCYC 1 cut(s) 436
BarI GAAGNNNNNNTAC 2 cut(s) 771, 803
BbrPI CACGTG 1 cut(s) 230
BbsI GAAGAC 2 cut(s) 128, 1065
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 4 cut(s) 420, 431, 592, 786
BccI CCATC 2 cut(s) 491, 1060
BciT130I CCWGG 4 cut(s) 50, 141, 583, 846
BciVI GTATCC 2 cut(s) 649, 723
BcnI CCSGG 2 cut(s) 24, 947
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 2 cut(s) 257, 876
BfmI CTRYAG 3 cut(s) 417, 667, 1107
BfoI RGCGCY 1 cut(s) 23
BfuI GTATCC 2 cut(s) 649, 723
BisI GCNGC 4 cut(s) 409, 420, 606, 800
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 4 cut(s) 410, 421, 607, 801
Bme1390I CCNGG 6 cut(s) 24, 50, 141, 583, 846, 947
Bme18I GGWCC 3 cut(s) 52, 450, 617
BmgBI CACGTC 2 cut(s) 812, 1048
BmgT120I GGNCC 5 cut(s) 52, 107, 450, 500, 617
BmiI GGNNCC 8 cut(s) 21, 54, 120, 435, 452, 502, 825, 843
BmrFI CCNGG 6 cut(s) 24, 50, 141, 583, 846, 947
BmsI GCATC 4 cut(s) 268, 361, 592, 811
BpiI GAAGAC 2 cut(s) 128, 1065
BplI GAGNNNNNCTC 2 cut(s) 545, 577
BpmI CTGGAG 2 cut(s) 565, 969
Bpu10I CCTNAGC 1 cut(s) 1214
Bpu1102I GCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 547
BpuMI CCSGG 2 cut(s) 24, 947
BsaAI YACGTR 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 291
BsaHI GRCGYC 2 cut(s) 9, 20
BsaJI CCNNGG 4 cut(s) 48, 319, 428, 927
BsaWI WCCGGW 2 cut(s) 485, 772
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 4 cut(s) 50, 141, 583, 846
BseDI CCNNGG 4 cut(s) 48, 319, 428, 927
BseGI GGATG 4 cut(s) 283, 314, 781, 937
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 4 cut(s) 362, 560, 1023, 1228
BseRI GAGGAG 1 cut(s) 114
BseXI GCAGC 4 cut(s) 420, 431, 592, 786
Bsh1236I CGCG 1 cut(s) 406
Bsh1285I CGRYCG 1 cut(s) 773
BshFI GGCC 4 cut(s) 108, 501, 578, 1219
BshNI GGYRCC 2 cut(s) 19, 118
BsiEI CGRYCG 1 cut(s) 773
BsiHKAI GWGCWC 1 cut(s) 733
BsiSI CCGG 4 cut(s) 24, 486, 773, 947
BslFI GGGAC 4 cut(s) 31, 38, 166, 751
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 548
BsmBI CGTCTC 1 cut(s) 548
BsmFI GGGAC 4 cut(s) 31, 38, 166, 751
BsmI GAATGC 2 cut(s) 260, 1162
BsnI GGCC 4 cut(s) 108, 501, 578, 1219
Bsp1286I GDGCHC 2 cut(s) 436, 733
Bsp1407I TGTACA 1 cut(s) 1185
Bsp143I GATC 6 cut(s) 66, 177, 371, 571, 910, 919
Bsp1720I GCTNAGC 1 cut(s) 348
BspANI GGCC 4 cut(s) 108, 501, 578, 1219
BspCNI CTCAG 4 cut(s) 361, 559, 1022, 1227
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 8 cut(s) 21, 54, 120, 435, 452, 502, 825, 843
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 4 cut(s) 61, 579, 905, 914
BspQI GCTCTTC 1 cut(s) 1158
BspT107I GGYRCC 2 cut(s) 19, 118
BsrDI GCAATG 1 cut(s) 429
BsrGI TGTACA 1 cut(s) 1185
BssECI CCNNGG 4 cut(s) 48, 319, 428, 927
BssMI GATC 6 cut(s) 66, 177, 371, 571, 910, 919
BssNI GRCGYC 2 cut(s) 9, 20
BssT1I CCWWGG 3 cut(s) 319, 428, 927
Bst2UI CCWGG 4 cut(s) 50, 141, 583, 846
Bst4CI ACNGT 3 cut(s) 671, 735, 1202
Bst6I CTCTTC 3 cut(s) 63, 734, 1158
BstACI GRCGYC 2 cut(s) 9, 20
BstAUI TGTACA 1 cut(s) 1185
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 729
BstDEI CTNAG 6 cut(s) 348, 546, 806, 1009, 1133, 1214
BstF5I GGATG 4 cut(s) 283, 314, 781, 937
BstFNI CGCG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 2 cut(s) 22, 408
BstKTI GATC 6 cut(s) 69, 180, 374, 574, 913, 922
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 6 cut(s) 66, 177, 371, 571, 910, 919
BstMCI CGRYCG 1 cut(s) 773
BstMWI GCNNNNNNNGC 2 cut(s) 155, 728
BstNI CCWGG 4 cut(s) 50, 141, 583, 846
BstNSI RCATGY 1 cut(s) 1106
BstSCI CCNGG 6 cut(s) 22, 48, 139, 581, 844, 945
BstSFI CTRYAG 3 cut(s) 417, 667, 1107
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 4 cut(s) 420, 431, 592, 786
BstV2I GAAGAC 2 cut(s) 128, 1065
BstX2I RGATCY 4 cut(s) 66, 571, 910, 919
BstXI CCANNNNNNTGG 1 cut(s) 234
BstYI RGATCY 4 cut(s) 66, 571, 910, 919
BsuI GTATCC 2 cut(s) 649, 723
BsuRI GGCC 4 cut(s) 108, 501, 578, 1219
BtrI CACGTC 2 cut(s) 812, 1048
BtsCI GGATG 4 cut(s) 283, 314, 781, 937
BtsI GCAGTG 1 cut(s) 92
BtsIMutI CAGTG 4 cut(s) 92, 534, 676, 1113
Cac8I GCNNGC 1 cut(s) 729
CciI TCATGA 1 cut(s) 244
CfoI GCGC 2 cut(s) 22, 408
Cfr13I GGNCC 5 cut(s) 52, 107, 450, 500, 617
CseI GACGC 2 cut(s) 565, 728
Csp6I GTAC 3 cut(s) 119, 312, 1186
CviAII CATG 8 cut(s) 181, 193, 245, 494, 506, 1063, 1103, 1172
CviQI GTAC 3 cut(s) 119, 312, 1186
DdeI CTNAG 6 cut(s) 348, 546, 806, 1009, 1133, 1214
DinI GGCGCC 1 cut(s) 21
DpnI GATC 6 cut(s) 68, 179, 373, 573, 912, 921
DpnII GATC 6 cut(s) 66, 177, 371, 571, 910, 919
Eam1104I CTCTTC 3 cut(s) 63, 734, 1158
EarI CTCTTC 3 cut(s) 63, 734, 1158
Eco130I CCWWGG 3 cut(s) 319, 428, 927
Eco147I AGGCCT 1 cut(s) 1219
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 3 cut(s) 52, 450, 617
Eco57I CTGAAG 2 cut(s) 594, 779
Eco72I CACGTG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 1118
EcoRII CCWGG 4 cut(s) 48, 139, 581, 844
EcoT14I CCWWGG 3 cut(s) 319, 428, 927
EcoT38I GRGCYC 1 cut(s) 436
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 3 cut(s) 319, 428, 927
Esp3I CGTCTC 1 cut(s) 548
FaeI CATG 8 cut(s) 184, 196, 248, 497, 509, 1066, 1106, 1175
FaqI GGGAC 4 cut(s) 31, 38, 166, 751
FatI CATG 8 cut(s) 180, 192, 244, 493, 505, 1062, 1102, 1171
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 4 cut(s) 409, 420, 606, 800
FokI GGATG 4 cut(s) 290, 301, 788, 944
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 4 cut(s) 409, 420, 606, 800
FspBI CTAG 2 cut(s) 257, 876
GlaI GCGC 2 cut(s) 21, 407
GluI GCNGC 4 cut(s) 409, 420, 606, 800
GsuI CTGGAG 2 cut(s) 565, 969
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 4 cut(s) 108, 501, 578, 1219
HapII CCGG 4 cut(s) 24, 486, 773, 947
HgaI GACGC 2 cut(s) 565, 728
HhaI GCGC 2 cut(s) 22, 408
Hin1I GRCGYC 2 cut(s) 9, 20
Hin1II CATG 8 cut(s) 184, 196, 248, 497, 509, 1066, 1106, 1175
Hin6I GCGC 2 cut(s) 20, 406
HinP1I GCGC 2 cut(s) 20, 406
HincII GTYRAC 2 cut(s) 588, 784
HindII GTYRAC 2 cut(s) 588, 784
HinfI GANTC 7 cut(s) 238, 253, 524, 635, 676, 998, 1086
HpaII CCGG 4 cut(s) 24, 486, 773, 947
HphI GGTGA 2 cut(s) 220, 716
Hpy166II GTNNAC 4 cut(s) 13, 532, 588, 784
Hpy188I TCNGA 4 cut(s) 997, 1012, 1197, 1209
Hpy8I GTNNAC 4 cut(s) 13, 532, 588, 784
Hpy99I CGWCG 1 cut(s) 1052
HpyAV CCTTC 3 cut(s) 190, 465, 1089
HpyCH4III ACNGT 3 cut(s) 671, 735, 1202
HpyCH4IV ACGT 3 cut(s) 229, 811, 1047
HpyCH4V TGCA 4 cut(s) 419, 802, 962, 1162
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 728
HpyF3I CTNAG 6 cut(s) 348, 546, 806, 1009, 1133, 1214
HpySE526I ACGT 3 cut(s) 229, 811, 1047
Hsp92I GRCGYC 2 cut(s) 9, 20
Hsp92II CATG 8 cut(s) 184, 196, 248, 497, 509, 1066, 1106, 1175
HspAI GCGC 2 cut(s) 20, 406
KasI GGCGCC 1 cut(s) 19
KpnI GGTACC 1 cut(s) 122
Kzo9I GATC 6 cut(s) 66, 177, 371, 571, 910, 919
LguI GCTCTTC 1 cut(s) 1158
LmnI GCTCC 2 cut(s) 439, 988
Lsp1109I GCAGC 4 cut(s) 420, 431, 592, 786
LweI GCATC 4 cut(s) 268, 361, 592, 811
MaeI CTAG 2 cut(s) 257, 876
MaeII ACGT 3 cut(s) 229, 811, 1047
MalI GATC 6 cut(s) 68, 179, 373, 573, 912, 921
MboI GATC 6 cut(s) 66, 177, 371, 571, 910, 919
MboII GAAGA 7 cut(s) 50, 128, 751, 785, 929, 1070, 1145
MflI RGATCY 4 cut(s) 66, 571, 910, 919
MhlI GDGCHC 2 cut(s) 436, 733
MluCI AATT 3 cut(s) 269, 1037, 1118
Mly113I GGCGCC 1 cut(s) 20
MmeI TCCRAC 2 cut(s) 428, 595
MseI TTAA 1 cut(s) 593
MslI CAYNNNNRTG 3 cut(s) 232, 306, 650
MspA1I CMGCKG 1 cut(s) 799
MspI CCGG 4 cut(s) 24, 486, 773, 947
MspR9I CCNGG 6 cut(s) 24, 50, 141, 583, 846, 947
Mva1269I GAATGC 2 cut(s) 260, 1162
MvaI CCWGG 4 cut(s) 50, 141, 583, 846
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 2 cut(s) 155, 728
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 2 cut(s) 24, 947
NdeII GATC 6 cut(s) 66, 177, 371, 571, 910, 919
NlaIII CATG 8 cut(s) 184, 196, 248, 497, 509, 1066, 1106, 1175
NlaIV GGNNCC 8 cut(s) 21, 54, 120, 435, 452, 502, 825, 843
NmeAIII GCCGAG 1 cut(s) 949
NspI RCATGY 1 cut(s) 1106
PagI TCATGA 1 cut(s) 244
PceI AGGCCT 1 cut(s) 1219
PciSI GCTCTTC 1 cut(s) 1158
PcsI WCGNNNNNNNCGW 1 cut(s) 361
PctI GAATGC 2 cut(s) 260, 1162
PfeI GAWTC 7 cut(s) 238, 253, 524, 635, 676, 998, 1086
PfoI TCCNGGA 1 cut(s) 139
PkrI GCNGC 4 cut(s) 410, 421, 607, 801
PluTI GGCGCC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 230
PmlI CACGTG 1 cut(s) 230
Ppu21I YACGTR 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 4 cut(s) 48, 139, 581, 844
PspCI CACGTG 1 cut(s) 230
PspGI CCWGG 4 cut(s) 48, 139, 581, 844
PspN4I GGNNCC 8 cut(s) 21, 54, 120, 435, 452, 502, 825, 843
PspPI GGNCC 5 cut(s) 52, 107, 450, 500, 617
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 4 cut(s) 66, 571, 910, 919
PvuII CAGCTG 1 cut(s) 799
RsaI GTAC 3 cut(s) 120, 313, 1187
RsaNI GTAC 3 cut(s) 119, 312, 1186
RseI CAYNNNNRTG 3 cut(s) 232, 306, 650
SapI GCTCTTC 1 cut(s) 1158
SaqAI TTAA 1 cut(s) 593
SatI GCNGC 4 cut(s) 409, 420, 606, 800
Sau3AI GATC 6 cut(s) 66, 177, 371, 571, 910, 919
Sau96I GGNCC 5 cut(s) 52, 107, 450, 500, 617
ScrFI CCNGG 6 cut(s) 24, 50, 141, 583, 846, 947
SduI GDGCHC 2 cut(s) 436, 733
SfaNI GCATC 4 cut(s) 268, 361, 592, 811
SfcI CTRYAG 3 cut(s) 417, 667, 1107
SfoI GGCGCC 1 cut(s) 21
SinI GGWCC 3 cut(s) 52, 450, 617
SmiMI CAYNNNNRTG 3 cut(s) 232, 306, 650
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 3 cut(s) 269, 1037, 1118
SseBI AGGCCT 1 cut(s) 1219
SspDI GGCGCC 1 cut(s) 19
SspI AATATT 1 cut(s) 1146
SspMI CTAG 2 cut(s) 257, 876
StuI AGGCCT 1 cut(s) 1219
StyD4I CCNGG 6 cut(s) 22, 48, 139, 581, 844, 945
StyI CCWWGG 3 cut(s) 319, 428, 927
TaaI ACNGT 3 cut(s) 671, 735, 1202
TaiI ACGT 3 cut(s) 232, 814, 1050
TaqI TCGA 3 cut(s) 264, 370, 829
TasI AATT 3 cut(s) 269, 1037, 1118
TatI WGTACW 1 cut(s) 1185
TfiI GAWTC 7 cut(s) 238, 253, 524, 635, 676, 998, 1086
Tru1I TTAA 1 cut(s) 593
Tru9I TTAA 1 cut(s) 593
TscAI CASTG 4 cut(s) 92, 534, 676, 1120
TseI GCWGC 4 cut(s) 408, 419, 605, 799
TspRI CASTG 4 cut(s) 92, 534, 676, 1120
VpaK11BI GGWCC 3 cut(s) 52, 450, 617
XapI RAATTY 3 cut(s) 269, 1037, 1118
XceI RCATGY 1 cut(s) 1106
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 2 cut(s) 257, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.