MD06G1074900.v1.1

Maspardin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012477
Physical Location & Seq
Reverse (-)
18656426 .. 18659393
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1074900.v1.1.491

Sequence Viewer

Length: 567 bp
ATGTATGACAAGGAGAGGTTTTGGGGGCGTAGAAAACCAGCAATGAATGGCGTCTACTCGCCGCCCGGAGATTACATCTACTTCAAGTCCCAGGTCCCTCTTCACAAGATCCCAATTGGCACGAAGCAGTGGCGATACTATGATTTTGGCCCGAAAGCAGTACCTCCTCTTATTTGTCTTCCTGGAACAGAAGGGATAGCTGATGTCTGCTACAAGCAGATCATGTCATTGTCCATGAAGGGTTATCGGGTGATTTCTGTTGATATTCCACGTGTATGGAATCGTCACGAGTGGATTCTAGCATTTGAGAAATTCTTGGATGCTATTGATGTCCATCACATACATATCTATGGTACAGCCCTTGGGGGCTTCTTGGCTCAACTTTTTGCTCAGCATCGTCCAACAAGAGTTCGGTCATTGATACTATCAAACACATTTTTAGACACGCGTAACTTCTCTGCAGCAATGCCATGGGCTCCCGTGTATGTTTGTCATATTTGTACTTTACACATTGATAAAGCTACAGGCATGCTGTTAAGTTGTGCCAAATCAAAAATTAGGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

189

Amino Acids

21.68

Weight (kDa)

9.55

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 55 - 147 4e-10 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12230
fragaria_vesca FvH4_5g00640 FvH4_5g00640 FvH4_5g00640
malus_domestica MD04G1079900.v1.1 MD06G1074900.v1.1
prunus_persica Prupe.5G079200_v2.0.a1
pyrus_communis pycom04g07200 pycom06g05350
rosa_chinensis RchiOBHm_Chr7g0201621
rosa_laevigata RLG00000003661
rosa_multiflora Rmu_sc0004811.1_g000001
rosa_roxburghii Rroxscaffold_3G00255190
rosa_rugosa Rorug07G0065200
rosa_samantha Rh7AG191900 Rh7BG194100 Rh7CG203100 Rh7DG197100
rosa_wichuraiana Rw7G016830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 448
AciI CCGC 1 cut(s) 62
AclWI GGATC 1 cut(s) 103
AcsI RAATTY 1 cut(s) 311
AcvI CACGTG 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 3 cut(s) 162, 355, 502
AflIII ACRYGT 2 cut(s) 271, 446
AgsI TTSAA 1 cut(s) 85
AjnI CCWGG 2 cut(s) 90, 181
AluBI AGCT 2 cut(s) 200, 521
AluI AGCT 2 cut(s) 200, 521
AlwI GGATC 1 cut(s) 103
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 1 cut(s) 311
AspS9I GGNCC 2 cut(s) 94, 149
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 478
BauI CACGAG 1 cut(s) 287
BbrPI CACGTG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 473
BccI CCATC 1 cut(s) 342
BciT130I CCWGG 2 cut(s) 92, 183
BcnI CCSGG 1 cut(s) 66
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 2 cut(s) 459, 522
BisI GCNGC 2 cut(s) 62, 462
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 2 cut(s) 63, 463
Bme1390I CCNGG 3 cut(s) 66, 92, 183
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 94, 149
BmiI GGNNCC 2 cut(s) 96, 477
BmrFI CCNGG 3 cut(s) 66, 92, 183
BmsI GCATC 2 cut(s) 310, 403
BpiI GAAGAC 1 cut(s) 170
Bpu1102I GCTNAGC 1 cut(s) 390
BpuMI CCSGG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 3 cut(s) 90, 361, 470
Bse3DI GCAATG 2 cut(s) 48, 471
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 2 cut(s) 92, 183
BseDI CCNNGG 3 cut(s) 90, 361, 470
BseGI GGATG 1 cut(s) 325
BseJI GATNNNNATC 1 cut(s) 333
BseMI GCAATG 2 cut(s) 48, 471
BseMII CTCAG 1 cut(s) 404
BseRI GAGGAG 1 cut(s) 156
BseXI GCAGC 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 1 cut(s) 150
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 2 cut(s) 73, 80
BsmFI GGGAC 2 cut(s) 73, 80
BsnI GGCC 1 cut(s) 150
Bsp1286I GDGCHC 1 cut(s) 478
Bsp143I GATC 2 cut(s) 108, 219
Bsp1720I GCTNAGC 1 cut(s) 390
Bsp19I CCATGG 1 cut(s) 470
BspACI CCGC 1 cut(s) 62
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 1 cut(s) 448
BspLI GGNNCC 2 cut(s) 96, 477
BspMAI CTGCAG 1 cut(s) 463
BspPI GGATC 1 cut(s) 103
BsrDI GCAATG 2 cut(s) 48, 471
BssECI CCNNGG 3 cut(s) 90, 361, 470
BssMI GATC 2 cut(s) 108, 219
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 287
BssT1I CCWWGG 2 cut(s) 361, 470
Bst2BI CACGAG 1 cut(s) 287
Bst2UI CCWGG 2 cut(s) 92, 183
Bst6I CTCTTC 1 cut(s) 105
BstACI GRCGYC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 1 cut(s) 470
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 1 cut(s) 448
BstKTI GATC 2 cut(s) 111, 222
BstMBI GATC 2 cut(s) 108, 219
BstNI CCWGG 2 cut(s) 92, 183
BstNSI RCATGY 1 cut(s) 532
BstSCI CCNGG 3 cut(s) 64, 90, 181
BstSFI CTRYAG 2 cut(s) 459, 522
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 1 cut(s) 473
BstV2I GAAGAC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 108
BstXI CCANNNNNNTGG 1 cut(s) 276
BstYI RGATCY 1 cut(s) 108
BsuRI GGCC 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 470
BtsCI GGATG 1 cut(s) 325
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 1 cut(s) 530
Cfr13I GGNCC 2 cut(s) 94, 149
CseI GACGC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 161, 354, 501
CviAII CATG 4 cut(s) 223, 235, 471, 529
CviJI RGCY 7 cut(s) 150, 200, 359, 369, 377, 476, 521
CviKI_1 RGCY 7 cut(s) 150, 200, 359, 369, 377, 476, 521
CviQI GTAC 3 cut(s) 161, 354, 501
DdeI CTNAG 1 cut(s) 390
DpnI GATC 2 cut(s) 110, 221
DpnII GATC 2 cut(s) 108, 219
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 361, 470
Eco24I GRGCYC 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 94
Eco72I CACGTG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 94
EcoRII CCWGG 2 cut(s) 90, 181
EcoT14I CCWWGG 2 cut(s) 361, 470
EcoT38I GRGCYC 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 361, 470
FaeI CATG 4 cut(s) 226, 238, 474, 532
FaqI GGGAC 2 cut(s) 73, 80
FatI CATG 4 cut(s) 222, 234, 470, 528
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 62, 462
FokI GGATG 1 cut(s) 332
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 2 cut(s) 62, 462
FspBI CTAG 1 cut(s) 299
GluI GCNGC 2 cut(s) 62, 462
HaeIII GGCC 1 cut(s) 150
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 4 cut(s) 226, 238, 474, 532
HinfI GANTC 2 cut(s) 280, 295
HpaII CCGG 1 cut(s) 66
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 55
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 55
HpyAV CCTTC 2 cut(s) 185, 232
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 1 cut(s) 461
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 1 cut(s) 271
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 4 cut(s) 226, 238, 474, 532
Kzo9I GATC 2 cut(s) 108, 219
LmnI GCTCC 1 cut(s) 481
LpnPI CCDG 7 cut(s) 51, 77, 79, 104, 168, 195, 510
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 2 cut(s) 310, 403
MaeI CTAG 1 cut(s) 299
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 284, 449
MalI GATC 2 cut(s) 110, 221
MboI GATC 2 cut(s) 108, 219
MboII GAAGA 2 cut(s) 92, 170
MfeI CAATTG 1 cut(s) 114
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 1 cut(s) 478
MluCI AATT 3 cut(s) 114, 311, 555
MluI ACGCGT 1 cut(s) 446
MmeI TCCRAC 1 cut(s) 425
MnlI CCTC 4 cut(s) 9, 108, 174, 177
MseI TTAA 1 cut(s) 536
MslI CAYNNNNRTG 2 cut(s) 274, 348
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 3 cut(s) 66, 92, 183
MunI CAATTG 1 cut(s) 114
MvaI CCWGG 2 cut(s) 92, 183
MvnI CGCG 1 cut(s) 448
NciI CCSGG 1 cut(s) 66
NcoI CCATGG 1 cut(s) 470
NdeII GATC 2 cut(s) 108, 219
NlaIII CATG 4 cut(s) 226, 238, 474, 532
NlaIV GGNNCC 2 cut(s) 96, 477
NmuCI GTSAC 1 cut(s) 284
NspI RCATGY 1 cut(s) 532
PaeI GCATGC 1 cut(s) 532
PfeI GAWTC 2 cut(s) 280, 295
PfoI TCCNGGA 1 cut(s) 181
PkrI GCNGC 2 cut(s) 63, 463
PmaCI CACGTG 1 cut(s) 272
PmlI CACGTG 1 cut(s) 272
Ppu21I YACGTR 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 94
Psp5II RGGWCCY 1 cut(s) 94
Psp6I CCWGG 2 cut(s) 90, 181
PspCI CACGTG 1 cut(s) 272
PspGI CCWGG 2 cut(s) 90, 181
PspN4I GGNNCC 2 cut(s) 96, 477
PspPI GGNCC 2 cut(s) 94, 149
PspPPI RGGWCCY 1 cut(s) 94
PstI CTGCAG 1 cut(s) 463
PsuI RGATCY 1 cut(s) 108
RsaI GTAC 3 cut(s) 162, 355, 502
RsaNI GTAC 3 cut(s) 161, 354, 501
RseI CAYNNNNRTG 2 cut(s) 274, 348
SaqAI TTAA 1 cut(s) 536
SatI GCNGC 2 cut(s) 62, 462
Sau3AI GATC 2 cut(s) 108, 219
Sau96I GGNCC 2 cut(s) 94, 149
ScrFI CCNGG 3 cut(s) 66, 92, 183
SduI GDGCHC 1 cut(s) 478
SetI ASST 7 cut(s) 20, 96, 166, 202, 274, 523, 563
SfaNI GCATC 2 cut(s) 310, 403
SfcI CTRYAG 2 cut(s) 459, 522
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 274, 348
SphI GCATGC 1 cut(s) 532
Sse9I AATT 3 cut(s) 114, 311, 555
SsiI CCGC 1 cut(s) 62
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 3 cut(s) 64, 90, 181
StyI CCWWGG 2 cut(s) 361, 470
TaiI ACGT 1 cut(s) 274
TaqII GACCGA 1 cut(s) 402
TasI AATT 3 cut(s) 114, 311, 555
TatI WGTACW 1 cut(s) 500
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 2 cut(s) 280, 295
Tru1I TTAA 1 cut(s) 536
Tru9I TTAA 1 cut(s) 536
TscAI CASTG 1 cut(s) 134
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 2 cut(s) 59, 251
TspRI CASTG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 94
XapI RAATTY 1 cut(s) 311
XceI RCATGY 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.