Rroxscaffold_3G00255190

Maspardin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012477
Physical Location & Seq
Forward (+)
49452347 .. 49458137
5791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255190.1

Sequence Viewer

Length: 1146 bp
ATGAAAGGCGTCTACTCGGCGCCCGGAGATTACATCTACTTCAAGTCCCAGGTCCCTCTTCACAAGATCCCAACCTATGTGACATGGGTCTGCGTCAAGTTTGTGTATTTTGATGTGAAGGGTTTTAATTCTGTCGAATTGTGGGGTTTTGAAATTGGCACAAAGCAGTGGCGATACTATGATTTTGGTCCGAAAGCAGTAACTCCACTTATATGTCTTCCTGGAACAGCAGGGACAGCAGATGTCTGTTACAAGCAGATCATGGCATTGTCCATGAAGGGTTACAGGGTCATTTCTGTTGATATTCCACGTGTATGGAATCATCAAGAATGGATTCAAGCATTTGAAAAGTTTTTGGATGCTATTGATGTTCATCATATACATCTCTATGGTACATCCCTTGGGGGCTTTTTGGCTCAACTTTTTGCTCAGCATCGTCCAAGACGGGTTCGATCGTTGGTACTATCAAATACCTTTCTGGACACACGCGACTTCTCTGCAGCAATGCCATGGGCTCCCATTGTTCCATACTCCGGTTCTTTATTAGTCATCAGACCCTGGGATGGCCTGGCAAGGGTTGTAGTGGTGCTCGAAGTGTGCGTTAGTTGGACCCCCTCTTTTTTGCTGAAAAGATACGTCTTATCAGGAATTCGTGATGGCCCCCACGAGCCATTTATTGCGGATTCAGTGGACTTTGTTGTTTCTCAGCTACACCTAAGACGAGTTGGTGTGGAAGCTCGACCTGACTTGGTTCCAGGCATCTCGAAGGGAGGTAGTGGTGGTGGGACCTCCAGTGAAGAGAATGAGAAAAAAGATCAGGACAAAAAAGATCCGGATGATCCATCCAAGGATGATCAGGGAAATTCAGAAAGTTCTAATGAAGAAAGTCAGATCCCACCACCCCCAGAAAGTTCAGAATCTCATAGCTTAGACGACCAGCTTGTCAATAATGCAAAGTTTTGCCCCTTGAGTAATGAAGACGTGGTCGTTTCTTTGCTTTCTGAATTCTTTAAGCAACAACACACTGTACCCCCTGAAATAGTTATGCGATTATGGGAAACGTTTGCTATTCATTTGCTTTCTTTATGTGTGGGGTCGTTGTACATTAGTTTGAACTGCAATCAGAAGCTTAGACAAGTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

381

Amino Acids

42.85

Weight (kDa)

6.01

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 69 - 162 3.4e-13 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12230
fragaria_vesca FvH4_5g00640 FvH4_5g00640 FvH4_5g00640
malus_domestica MD04G1079900.v1.1 MD06G1074900.v1.1
prunus_persica Prupe.5G079200_v2.0.a1
pyrus_communis pycom04g07200 pycom06g05350
rosa_chinensis RchiOBHm_Chr7g0201621
rosa_laevigata RLG00000003661
rosa_multiflora Rmu_sc0004811.1_g000001
rosa_roxburghii Rroxscaffold_3G00255190
rosa_rugosa Rorug07G0065200
rosa_samantha Rh7AG191900 Rh7BG194100 Rh7CG203100 Rh7DG197100
rosa_wichuraiana Rw7G016830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 941
AccB1I GGYRCC 1 cut(s) 19
AccI GTMKAC 1 cut(s) 12
AccII CGCG 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 832
AciI CCGC 1 cut(s) 680
AclI AACGTT 1 cut(s) 1061
AclWI GGATC 4 cut(s) 61, 824, 833, 886
AcsI RAATTY 3 cut(s) 648, 862, 1004
AcvI CACGTG 1 cut(s) 311
AcyI GRCGYC 2 cut(s) 9, 20
AfaI GTAC 4 cut(s) 394, 462, 1029, 1103
AfiI CCNNNNNNNGG 3 cut(s) 533, 563, 574
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 5 cut(s) 43, 152, 338, 347, 1114
AjiI CACGTC 1 cut(s) 982
AjnI CCWGG 5 cut(s) 48, 220, 557, 567, 754
AluBI AGCT 5 cut(s) 709, 737, 927, 940, 1129
AluI AGCT 5 cut(s) 709, 737, 927, 940, 1129
Alw21I GWGCWC 1 cut(s) 591
AlwI GGATC 4 cut(s) 61, 824, 833, 886
AlwNI CAGNNNCTG 1 cut(s) 558
Aor13HI TCCGGA 1 cut(s) 832
AoxI GGCC 2 cut(s) 565, 658
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 3 cut(s) 648, 862, 1004
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 5 cut(s) 52, 188, 609, 659, 786
AsuC2I CCSGG 1 cut(s) 24
AvaII GGWCC 4 cut(s) 52, 188, 609, 786
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 517
BauI CACGAG 1 cut(s) 665
BbrPI CACGTG 1 cut(s) 311
BbsI GAAGAC 2 cut(s) 209, 984
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 512
BccI CCATC 3 cut(s) 557, 650, 850
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciT130I CCWGG 5 cut(s) 50, 222, 559, 569, 756
BclI TGATCA 1 cut(s) 853
BcnI CCSGG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 498
BfoI RGCGCY 1 cut(s) 23
BisI GCNGC 1 cut(s) 501
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 6 cut(s) 24, 50, 222, 559, 569, 756
Bme18I GGWCC 4 cut(s) 52, 188, 609, 786
BmgBI CACGTC 1 cut(s) 982
BmgT120I GGNCC 5 cut(s) 52, 188, 609, 659, 786
BmiI GGNNCC 7 cut(s) 21, 54, 516, 611, 661, 753, 787
BmrFI CCNGG 6 cut(s) 24, 50, 222, 559, 569, 756
BmsI GCATC 3 cut(s) 349, 442, 768
BoxI GACNNNNGTC 1 cut(s) 86
BpiI GAAGAC 2 cut(s) 209, 984
BpmI CTGGAG 1 cut(s) 775
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 988
BpuMI CCSGG 1 cut(s) 24
BsaAI YACGTR 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 372
BsaHI GRCGYC 2 cut(s) 9, 20
BsaJI CCNNGG 6 cut(s) 48, 400, 509, 557, 558, 846
BsaWI WCCGGW 2 cut(s) 533, 832
Bsc4I CCNNNNNNNGG 3 cut(s) 533, 563, 574
Bse1I ACTGG 1 cut(s) 792
Bse3DI GCAATG 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 372
BseAI TCCGGA 1 cut(s) 832
BseBI CCWGG 5 cut(s) 50, 222, 559, 569, 756
BseDI CCNNGG 6 cut(s) 48, 400, 509, 557, 558, 846
BseGI GGATG 6 cut(s) 364, 395, 568, 841, 842, 856
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 3 cut(s) 533, 563, 574
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 443, 719
BseNI ACTGG 1 cut(s) 792
BseXI GCAGC 1 cut(s) 512
Bsh1236I CGCG 1 cut(s) 489
Bsh1285I CGRYCG 1 cut(s) 455
BshFI GGCC 2 cut(s) 567, 660
BshNI GGYRCC 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 3 cut(s) 24, 534, 833
BslFI GGGAC 4 cut(s) 31, 38, 247, 799
BslI CCNNNNNNNGG 3 cut(s) 533, 563, 574
BsmFI GGGAC 4 cut(s) 31, 38, 247, 799
BsnI GGCC 2 cut(s) 567, 660
Bsp1286I GDGCHC 2 cut(s) 517, 591
Bsp13I TCCGGA 1 cut(s) 832
Bsp1407I TGTACA 1 cut(s) 1101
Bsp143I GATC 8 cut(s) 66, 258, 452, 814, 829, 838, 853, 891
Bsp1720I GCTNAGC 1 cut(s) 429
Bsp19I CCATGG 1 cut(s) 509
BspACI CCGC 1 cut(s) 680
BspANI GGCC 2 cut(s) 567, 660
BspCNI CTCAG 2 cut(s) 442, 718
BspEI TCCGGA 1 cut(s) 832
BspFNI CGCG 1 cut(s) 489
BspLI GGNNCC 7 cut(s) 21, 54, 516, 611, 661, 753, 787
BspMAI CTGCAG 1 cut(s) 502
BspPI GGATC 4 cut(s) 61, 824, 833, 886
BspT107I GGYRCC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 510
BsrGI TGTACA 1 cut(s) 1101
BsrI ACTGG 1 cut(s) 792
BssECI CCNNGG 6 cut(s) 48, 400, 509, 557, 558, 846
BssMI GATC 8 cut(s) 66, 258, 452, 814, 829, 838, 853, 891
BssNI GRCGYC 2 cut(s) 9, 20
BssSI CACGAG 1 cut(s) 665
BssT1I CCWWGG 3 cut(s) 400, 509, 846
Bst2BI CACGAG 1 cut(s) 665
Bst2UI CCWGG 5 cut(s) 50, 222, 559, 569, 756
Bst4CI ACNGT 1 cut(s) 1027
Bst6I CTCTTC 2 cut(s) 63, 792
BstACI GRCGYC 2 cut(s) 9, 20
BstAUI TGTACA 1 cut(s) 1101
BstBAI YACGTR 1 cut(s) 311
BstDEI CTNAG 5 cut(s) 429, 705, 716, 928, 1130
BstDSI CCRYGG 1 cut(s) 509
BstF5I GGATG 6 cut(s) 364, 395, 568, 841, 842, 856
BstFNI CGCG 1 cut(s) 489
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 8 cut(s) 69, 261, 455, 817, 832, 841, 856, 894
BstMBI GATC 8 cut(s) 66, 258, 452, 814, 829, 838, 853, 891
BstMCI CGRYCG 1 cut(s) 455
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 5 cut(s) 50, 222, 559, 569, 756
BstPAI GACNNNNGTC 1 cut(s) 86
BstSCI CCNGG 6 cut(s) 22, 48, 220, 557, 567, 754
BstSFI CTRYAG 1 cut(s) 498
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 2 cut(s) 209, 984
BstX2I RGATCY 3 cut(s) 66, 829, 891
BstXI CCANNNNNNTGG 1 cut(s) 315
BstYI RGATCY 3 cut(s) 66, 829, 891
BsuRI GGCC 2 cut(s) 567, 660
BtgI CCRYGG 1 cut(s) 509
BtrI CACGTC 1 cut(s) 982
BtsCI GGATG 6 cut(s) 364, 395, 568, 841, 842, 856
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 4 cut(s) 173, 693, 799, 1023
CaiI CAGNNNCTG 1 cut(s) 558
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 5 cut(s) 52, 188, 609, 659, 786
CseI GACGC 1 cut(s) 82
Csp6I GTAC 4 cut(s) 393, 461, 1028, 1102
CviAII CATG 4 cut(s) 84, 262, 274, 510
CviQI GTAC 4 cut(s) 393, 461, 1028, 1102
DdeI CTNAG 5 cut(s) 429, 705, 716, 928, 1130
DinI GGCGCC 1 cut(s) 21
DpnI GATC 8 cut(s) 68, 260, 454, 816, 831, 840, 855, 893
DpnII GATC 8 cut(s) 66, 258, 452, 814, 829, 838, 853, 891
DrdI GACNNNNNNGTC 1 cut(s) 941
DseDI GACNNNNNNGTC 1 cut(s) 941
Eam1104I CTCTTC 2 cut(s) 63, 792
EarI CTCTTC 2 cut(s) 63, 792
Eco130I CCWWGG 3 cut(s) 400, 509, 846
Eco24I GRGCYC 1 cut(s) 517
Eco47I GGWCC 4 cut(s) 52, 188, 609, 786
Eco72I CACGTG 1 cut(s) 311
EcoO109I RGGNCCY 2 cut(s) 52, 786
EcoRI GAATTC 2 cut(s) 648, 1004
EcoRII CCWGG 5 cut(s) 48, 220, 557, 567, 754
EcoT14I CCWWGG 3 cut(s) 400, 509, 846
EcoT38I GRGCYC 1 cut(s) 517
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 3 cut(s) 400, 509, 846
FaeI CATG 4 cut(s) 87, 265, 277, 513
FaqI GGGAC 4 cut(s) 31, 38, 247, 799
FatI CATG 4 cut(s) 83, 261, 273, 509
FbaI TGATCA 1 cut(s) 853
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 6 cut(s) 371, 382, 575, 829, 848, 863
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 1 cut(s) 501
GlaI GCGC 1 cut(s) 21
GluI GCNGC 1 cut(s) 501
GsuI CTGGAG 1 cut(s) 775
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 2 cut(s) 567, 660
HapII CCGG 3 cut(s) 24, 534, 833
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 22
Hin1I GRCGYC 2 cut(s) 9, 20
Hin1II CATG 4 cut(s) 87, 265, 277, 513
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HindIII AAGCTT 1 cut(s) 1127
HinfI GANTC 4 cut(s) 319, 334, 683, 917
HpaII CCGG 3 cut(s) 24, 534, 833
Hpy166II GTNNAC 2 cut(s) 13, 691
Hpy188I TCNGA 7 cut(s) 192, 554, 868, 891, 916, 1003, 1125
Hpy188III TCNNGA 7 cut(s) 326, 479, 645, 653, 763, 818, 833
Hpy8I GTNNAC 2 cut(s) 13, 691
HpyAV CCTTC 3 cut(s) 112, 271, 760
HpyCH4III ACNGT 1 cut(s) 1027
HpyCH4IV ACGT 4 cut(s) 310, 636, 981, 1061
HpyCH4V TGCA 3 cut(s) 500, 953, 1119
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 5 cut(s) 429, 705, 716, 928, 1130
HpySE526I ACGT 4 cut(s) 310, 636, 981, 1061
Hsp92I GRCGYC 2 cut(s) 9, 20
Hsp92II CATG 4 cut(s) 87, 265, 277, 513
HspAI GCGC 1 cut(s) 20
KasI GGCGCC 1 cut(s) 19
Kpn2I TCCGGA 1 cut(s) 832
Ksp22I TGATCA 1 cut(s) 853
Kzo9I GATC 8 cut(s) 66, 258, 452, 814, 829, 838, 853, 891
LmnI GCTCC 1 cut(s) 520
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 3 cut(s) 349, 442, 768
MaeII ACGT 4 cut(s) 310, 636, 981, 1061
MaeIII GTNAC 4 cut(s) 79, 199, 248, 281
MalI GATC 8 cut(s) 68, 260, 454, 816, 831, 840, 855, 893
MboI GATC 8 cut(s) 66, 258, 452, 814, 829, 838, 853, 891
MboII GAAGA 5 cut(s) 50, 209, 809, 893, 989
MflI RGATCY 3 cut(s) 66, 829, 891
MhlI GDGCHC 2 cut(s) 517, 591
MluCI AATT 6 cut(s) 127, 137, 153, 648, 862, 1004
Mly113I GGCGCC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 587
MnlI CCTC 4 cut(s) 66, 625, 764, 799
MroI TCCGGA 1 cut(s) 832
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 2 cut(s) 126, 1011
MslI CAYNNNNRTG 3 cut(s) 211, 313, 387
MspI CCGG 3 cut(s) 24, 534, 833
MspR9I CCNGG 6 cut(s) 24, 50, 222, 559, 569, 756
MvaI CCWGG 5 cut(s) 50, 222, 559, 569, 756
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 1 cut(s) 236
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 1 cut(s) 24
NcoI CCATGG 1 cut(s) 509
NdeII GATC 8 cut(s) 66, 258, 452, 814, 829, 838, 853, 891
NlaIII CATG 4 cut(s) 87, 265, 277, 513
NlaIV GGNNCC 7 cut(s) 21, 54, 516, 611, 661, 753, 787
NmuCI GTSAC 1 cut(s) 79
PasI CCCWGGG 1 cut(s) 558
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 4 cut(s) 319, 334, 683, 917
PflFI GACNNNGTC 1 cut(s) 983
PfoI TCCNGGA 1 cut(s) 220
PkrI GCNGC 1 cut(s) 502
Ple19I CGATCG 1 cut(s) 455
PluTI GGCGCC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 311
PmlI CACGTG 1 cut(s) 311
Ppu21I YACGTR 1 cut(s) 311
PpuMI RGGWCCY 2 cut(s) 52, 786
PshAI GACNNNNGTC 1 cut(s) 86
Psp1406I AACGTT 1 cut(s) 1061
Psp5II RGGWCCY 2 cut(s) 52, 786
Psp6I CCWGG 5 cut(s) 48, 220, 557, 567, 754
PspCI CACGTG 1 cut(s) 311
PspGI CCWGG 5 cut(s) 48, 220, 557, 567, 754
PspN4I GGNNCC 7 cut(s) 21, 54, 516, 611, 661, 753, 787
PspPI GGNCC 5 cut(s) 52, 188, 609, 659, 786
PspPPI RGGWCCY 2 cut(s) 52, 786
PstI CTGCAG 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 558
PsuI RGATCY 3 cut(s) 66, 829, 891
PsyI GACNNNGTC 1 cut(s) 983
PvuI CGATCG 1 cut(s) 455
RsaI GTAC 4 cut(s) 394, 462, 1029, 1103
RsaNI GTAC 4 cut(s) 393, 461, 1028, 1102
RseI CAYNNNNRTG 3 cut(s) 211, 313, 387
SaqAI TTAA 2 cut(s) 126, 1011
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 8 cut(s) 66, 258, 452, 814, 829, 838, 853, 891
Sau96I GGNCC 5 cut(s) 52, 188, 609, 659, 786
ScrFI CCNGG 6 cut(s) 24, 50, 222, 559, 569, 756
SduI GDGCHC 2 cut(s) 517, 591
SfaNI GCATC 3 cut(s) 349, 442, 768
SfcI CTRYAG 1 cut(s) 498
SfoI GGCGCC 1 cut(s) 21
SinI GGWCC 4 cut(s) 52, 188, 609, 786
SmiMI CAYNNNNRTG 3 cut(s) 211, 313, 387
SmlI CTYRAG 1 cut(s) 967
SmoI CTYRAG 1 cut(s) 967
Sse9I AATT 6 cut(s) 127, 137, 153, 648, 862, 1004
SsiI CCGC 1 cut(s) 680
SspDI GGCGCC 1 cut(s) 19
StyD4I CCNGG 6 cut(s) 22, 48, 220, 557, 567, 754
StyI CCWWGG 3 cut(s) 400, 509, 846
TaaI ACNGT 1 cut(s) 1027
TaiI ACGT 4 cut(s) 313, 639, 984, 1064
TaqI TCGA 5 cut(s) 135, 451, 591, 739, 764
TasI AATT 6 cut(s) 127, 137, 153, 648, 862, 1004
TatI WGTACW 1 cut(s) 1101
TfiI GAWTC 4 cut(s) 319, 334, 683, 917
Tru1I TTAA 2 cut(s) 126, 1011
Tru9I TTAA 2 cut(s) 126, 1011
TscAI CASTG 4 cut(s) 173, 693, 799, 1030
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 6 cut(s) 17, 290, 362, 894, 990, 1061
TspRI CASTG 4 cut(s) 173, 693, 799, 1030
Tth111I GACNNNGTC 1 cut(s) 983
VpaK11BI GGWCC 4 cut(s) 52, 188, 609, 786
XapI RAATTY 3 cut(s) 648, 862, 1004
XmiI GTMKAC 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.