Rh7DG197100

Maspardin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012477
Physical Location & Seq
Reverse (-)
18517359 .. 18523133
5775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG197100.1

Sequence Viewer

Length: 1056 bp
ATGGCATTGTCCATGAAGGGTTACAGGGTCATTTCTGTTGATATTCCACGTGTATGGAATCATCAAGAATGGATTCAAGCATTTGAAAAGTTCTTGGATGCTATTGATGTTCATCATATACATCTCTATGGTACATCCCTTGGGGGCTTTTTGGCTCAACTTTTTGCTCAGCATCGTCCAAGACGGGTTCGATCGTTGATACTATCAAATACCTTTCTGGACACACGCGACTTCTCTGCAGCAATGCCATGGGCTCCCATTGTTAGTTGGACCCCCTCTTTTTTGCTGAAAAGATACGTCTTATCAGGAATTCATGATGGCCCCCACGAGCCATTTATTGCAGATTCAGTGGACTTTGTTGTTTCTCAGGTTGAAACACTCTCAAGGGAAGACCTGGCCTCCAGGTTGACTTTAACAGCTGATGCTGCACAAGTTGGACCTCTTTTGCTCACAGACTCATTCATCACTATAATGGATACAAATGACTACAGTGCGATTCCTCAACAACTCAAAGATCAACTGAGTGAAAGGTACCCTGAAGCAAGGCGTGCACAATTAAAGACGGGGGGAGATTTTCCATTTCTTTCACGCCCAGATGAAGTCAACTTACATCTTCAGCTACACCTAAGACGAGTTGGTGTGGAAGCTCGACCTGACTTGGTTCCGGGCATCTCGAAGGGAGGTAGTGGTGGTGGGACCTCCAGTGAAGAGAATGAGAAAAAAGATAAGGACAAAAAAGATCCGGATGATCCATCCAAGGATGATCAGGGAAATTCAGAAAGTTCTAATGAAGAAAGTCAGATCCCACCACCCCCAGAAAGTTCAGAATCTCATAGCTTAGACGACCAGCTTGTCAACAATGCAAAGTTTTGCCCCTTGAGTAATGAAGACGTGGCCGTTTCCTTGCTTTCTGAATTCTTTAAGCAACAACACACTGTACCTCCTGAAATAGTTATGCGATTATGGGAAACGTTTGCAATTTATTTGCTTTCTTTATGTGTGGGATCGTTGTACATTAGTTTGAACTGTAATCAGAAGCTTAGACTAGTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

351

Amino Acids

39.36

Weight (kDa)

5.23

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 6 - 201 4.7e-07 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 17 - 94 4.7e-10 alpha/beta hydrolase fold
Hydrolase_4 PF12146 25 - 101 3.8e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12230
fragaria_vesca FvH4_5g00640 FvH4_5g00640 FvH4_5g00640
malus_domestica MD04G1079900.v1.1 MD06G1074900.v1.1
prunus_persica Prupe.5G079200_v2.0.a1
pyrus_communis pycom04g07200 pycom06g05350
rosa_chinensis RchiOBHm_Chr7g0201621
rosa_laevigata RLG00000003661
rosa_multiflora Rmu_sc0004811.1_g000001
rosa_roxburghii Rroxscaffold_3G00255190
rosa_rugosa Rorug07G0065200
rosa_samantha Rh7AG191900 Rh7BG194100 Rh7CG203100 Rh7DG197100
rosa_wichuraiana Rw7G016830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 531
AccII CGCG 1 cut(s) 228
AccIII TCCGGA 1 cut(s) 742
AclI AACGTT 1 cut(s) 971
AclWI GGATC 4 cut(s) 734, 743, 796, 1012
AcoI YGGCCR 1 cut(s) 894
AcsI RAATTY 3 cut(s) 309, 772, 914
AcuI CTGAAG 2 cut(s) 558, 599
AcvI CACGTG 1 cut(s) 50
AfaI GTAC 4 cut(s) 133, 533, 939, 1013
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 4 cut(s) 77, 86, 374, 1024
AhlI ACTAGT 1 cut(s) 1045
AjiI CACGTC 1 cut(s) 892
AjnI CCWGG 2 cut(s) 393, 401
AluBI AGCT 6 cut(s) 419, 619, 647, 837, 850, 1039
AluI AGCT 6 cut(s) 419, 619, 647, 837, 850, 1039
Alw21I GWGCWC 1 cut(s) 553
Alw44I GTGCAC 1 cut(s) 549
AlwI GGATC 4 cut(s) 734, 743, 796, 1012
Aor13HI TCCGGA 1 cut(s) 742
AoxI GGCC 3 cut(s) 319, 396, 894
ApaLI GTGCAC 1 cut(s) 549
ApeKI GCWGC 2 cut(s) 239, 425
ApoI RAATTY 3 cut(s) 309, 772, 914
Asp700I GAANNNNTTC 1 cut(s) 72
Asp718I GGTACC 1 cut(s) 531
AspS9I GGNCC 4 cut(s) 270, 320, 437, 696
AsuC2I CCSGG 1 cut(s) 666
AvaII GGWCC 3 cut(s) 270, 437, 696
BaeGI GKGCMC 1 cut(s) 553
BanI GGYRCC 1 cut(s) 531
BanII GRGCYC 1 cut(s) 256
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
BauI CACGAG 1 cut(s) 326
BbrPI CACGTG 1 cut(s) 50
BbsI GAAGAC 2 cut(s) 396, 894
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 2 cut(s) 251, 412
BccI CCATC 2 cut(s) 311, 760
BceAI ACGGC 1 cut(s) 881
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BciT130I CCWGG 2 cut(s) 395, 403
BciVI GTATCC 1 cut(s) 469
BclI TGATCA 1 cut(s) 763
BcnI CCSGG 1 cut(s) 666
BcuI ACTAGT 1 cut(s) 1045
BfaI CTAG 1 cut(s) 1046
BfmI CTRYAG 2 cut(s) 237, 487
BfuI GTATCC 1 cut(s) 469
BisI GCNGC 2 cut(s) 240, 426
BlpI GCTNAGC 1 cut(s) 168
BlsI GCNGC 2 cut(s) 241, 427
Bme1390I CCNGG 3 cut(s) 395, 403, 666
Bme18I GGWCC 3 cut(s) 270, 437, 696
BmgBI CACGTC 1 cut(s) 892
BmgT120I GGNCC 4 cut(s) 270, 320, 437, 696
BmiI GGNNCC 6 cut(s) 255, 272, 322, 533, 663, 697
BmrFI CCNGG 3 cut(s) 395, 403, 666
BmsI GCATC 4 cut(s) 88, 181, 412, 678
BpiI GAAGAC 2 cut(s) 396, 894
BpmI CTGGAG 2 cut(s) 385, 685
Bpu1102I GCTNAGC 1 cut(s) 168
BpuEI CTTGAG 2 cut(s) 367, 898
BpuMI CCSGG 1 cut(s) 666
BsaAI YACGTR 1 cut(s) 50
BsaBI GATNNNNATC 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 139, 248, 756
BsaWI WCCGGW 1 cut(s) 742
BsaXI ACNNNNNCTCC 4 cut(s) 383, 413, 925, 955
Bse1I ACTGG 1 cut(s) 702
Bse3DI GCAATG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 111
BseAI TCCGGA 1 cut(s) 742
BseBI CCWGG 2 cut(s) 395, 403
BseDI CCNNGG 3 cut(s) 139, 248, 756
BseGI GGATG 5 cut(s) 103, 134, 751, 752, 766
BseJI GATNNNNATC 1 cut(s) 111
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 3 cut(s) 182, 380, 512
BseNI ACTGG 1 cut(s) 702
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 2 cut(s) 251, 412
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 1 cut(s) 228
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 3 cut(s) 321, 398, 896
BshNI GGYRCC 1 cut(s) 531
BsiEI CGRYCG 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 2 cut(s) 665, 743
BslFI GGGAC 1 cut(s) 709
BsmFI GGGAC 1 cut(s) 709
BsnI GGCC 3 cut(s) 321, 398, 896
Bsp1286I GDGCHC 2 cut(s) 256, 553
Bsp13I TCCGGA 1 cut(s) 742
Bsp1407I TGTACA 1 cut(s) 1011
Bsp143I GATC 7 cut(s) 191, 514, 739, 748, 763, 801, 1004
Bsp1720I GCTNAGC 1 cut(s) 168
Bsp19I CCATGG 1 cut(s) 248
BspANI GGCC 3 cut(s) 321, 398, 896
BspCNI CTCAG 3 cut(s) 181, 379, 513
BspEI TCCGGA 1 cut(s) 742
BspFNI CGCG 1 cut(s) 228
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 6 cut(s) 255, 272, 322, 533, 663, 697
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 4 cut(s) 734, 743, 796, 1012
BspT107I GGYRCC 1 cut(s) 531
BsrDI GCAATG 1 cut(s) 249
BsrGI TGTACA 1 cut(s) 1011
BsrI ACTGG 1 cut(s) 702
BssECI CCNNGG 3 cut(s) 139, 248, 756
BssMI GATC 7 cut(s) 191, 514, 739, 748, 763, 801, 1004
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 3 cut(s) 139, 248, 756
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 2 cut(s) 395, 403
Bst4CI ACNGT 3 cut(s) 491, 937, 1028
Bst6I CTCTTC 1 cut(s) 702
BstAPI GCANNNNNTGC 1 cut(s) 548
BstAUI TGTACA 1 cut(s) 1011
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 549
BstDEI CTNAG 6 cut(s) 168, 366, 521, 626, 838, 1040
BstDSI CCRYGG 1 cut(s) 248
BstF5I GGATG 5 cut(s) 103, 134, 751, 752, 766
BstFNI CGCG 1 cut(s) 228
BstKTI GATC 7 cut(s) 194, 517, 742, 751, 766, 804, 1007
BstMBI GATC 7 cut(s) 191, 514, 739, 748, 763, 801, 1004
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 2 cut(s) 425, 548
BstNI CCWGG 2 cut(s) 395, 403
BstSCI CCNGG 3 cut(s) 393, 401, 664
BstSFI CTRYAG 2 cut(s) 237, 487
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 251, 412
BstV2I GAAGAC 2 cut(s) 396, 894
BstX2I RGATCY 2 cut(s) 739, 801
BstXI CCANNNNNNTGG 1 cut(s) 54
BstYI RGATCY 2 cut(s) 739, 801
BsuI GTATCC 1 cut(s) 469
BsuRI GGCC 3 cut(s) 321, 398, 896
BtgI CCRYGG 1 cut(s) 248
BtrI CACGTC 1 cut(s) 892
BtsCI GGATG 5 cut(s) 103, 134, 751, 752, 766
BtsIMutI CAGTG 4 cut(s) 354, 496, 709, 933
Cac8I GCNNGC 1 cut(s) 549
CciI TCATGA 1 cut(s) 313
Cfr13I GGNCC 4 cut(s) 270, 320, 437, 696
Csp6I GTAC 4 cut(s) 132, 532, 938, 1012
CviAII CATG 3 cut(s) 13, 249, 314
CviQI GTAC 4 cut(s) 132, 532, 938, 1012
DdeI CTNAG 6 cut(s) 168, 366, 521, 626, 838, 1040
DpnI GATC 7 cut(s) 193, 516, 741, 750, 765, 803, 1006
DpnII GATC 7 cut(s) 191, 514, 739, 748, 763, 801, 1004
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 894
Eam1104I CTCTTC 1 cut(s) 702
EarI CTCTTC 1 cut(s) 702
Eco130I CCWWGG 3 cut(s) 139, 248, 756
Eco24I GRGCYC 1 cut(s) 256
Eco47I GGWCC 3 cut(s) 270, 437, 696
Eco57I CTGAAG 2 cut(s) 558, 599
Eco72I CACGTG 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 696
EcoRI GAATTC 2 cut(s) 309, 914
EcoRII CCWGG 2 cut(s) 393, 401
EcoT14I CCWWGG 3 cut(s) 139, 248, 756
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 3 cut(s) 139, 248, 756
FaeI CATG 3 cut(s) 16, 252, 317
FaqI GGGAC 1 cut(s) 709
FatI CATG 3 cut(s) 12, 248, 313
FbaI TGATCA 1 cut(s) 763
Fnu4HI GCNGC 2 cut(s) 240, 426
FokI GGATG 5 cut(s) 110, 121, 739, 758, 773
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 2 cut(s) 240, 426
FspBI CTAG 1 cut(s) 1046
GluI GCNGC 2 cut(s) 240, 426
GsuI CTGGAG 2 cut(s) 385, 685
HaeIII GGCC 3 cut(s) 321, 398, 896
HapII CCGG 2 cut(s) 665, 743
Hin1II CATG 3 cut(s) 16, 252, 317
HincII GTYRAC 3 cut(s) 408, 604, 856
HindII GTYRAC 3 cut(s) 408, 604, 856
HindIII AAGCTT 1 cut(s) 1037
HinfI GANTC 6 cut(s) 58, 73, 344, 455, 496, 827
HpaII CCGG 2 cut(s) 665, 743
Hpy166II GTNNAC 5 cut(s) 352, 408, 551, 604, 856
Hpy188I TCNGA 5 cut(s) 778, 801, 826, 913, 1035
Hpy188III TCNNGA 7 cut(s) 65, 218, 306, 314, 673, 743, 944
Hpy8I GTNNAC 5 cut(s) 352, 408, 551, 604, 856
HpyAV CCTTC 2 cut(s) 10, 670
HpyCH4III ACNGT 3 cut(s) 491, 937, 1028
HpyCH4IV ACGT 4 cut(s) 49, 297, 891, 971
HpyCH4V TGCA 6 cut(s) 239, 341, 428, 551, 863, 977
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 548
HpyF3I CTNAG 6 cut(s) 168, 366, 521, 626, 838, 1040
HpySE526I ACGT 4 cut(s) 49, 297, 891, 971
Hsp92II CATG 3 cut(s) 16, 252, 317
Kpn2I TCCGGA 1 cut(s) 742
KpnI GGTACC 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 7 cut(s) 191, 514, 739, 748, 763, 801, 1004
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 2 cut(s) 251, 412
LweI GCATC 4 cut(s) 88, 181, 412, 678
MaeI CTAG 1 cut(s) 1046
MaeII ACGT 4 cut(s) 49, 297, 891, 971
MaeIII GTNAC 1 cut(s) 20
MalI GATC 7 cut(s) 193, 516, 741, 750, 765, 803, 1006
MboI GATC 7 cut(s) 191, 514, 739, 748, 763, 801, 1004
MboII GAAGA 5 cut(s) 401, 605, 719, 803, 899
MflI RGATCY 2 cut(s) 739, 801
MhlI GDGCHC 2 cut(s) 256, 553
MluCI AATT 5 cut(s) 309, 554, 772, 914, 978
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 248, 415
MnlI CCTC 7 cut(s) 286, 409, 450, 510, 674, 709, 951
MroI TCCGGA 1 cut(s) 742
MroXI GAANNNNTTC 1 cut(s) 72
MseI TTAA 3 cut(s) 413, 557, 921
MslI CAYNNNNRTG 3 cut(s) 52, 126, 470
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 2 cut(s) 665, 743
MspR9I CCNGG 3 cut(s) 395, 403, 666
MvaI CCWGG 2 cut(s) 395, 403
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 425, 548
NciI CCSGG 1 cut(s) 666
NcoI CCATGG 1 cut(s) 248
NdeII GATC 7 cut(s) 191, 514, 739, 748, 763, 801, 1004
NlaIII CATG 3 cut(s) 16, 252, 317
NlaIV GGNNCC 6 cut(s) 255, 272, 322, 533, 663, 697
PagI TCATGA 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 5 cut(s) 58, 73, 344, 496, 827
PkrI GCNGC 2 cut(s) 241, 427
Ple19I CGATCG 1 cut(s) 194
PleI GAGTC 1 cut(s) 449
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 1 cut(s) 449
Ppu21I YACGTR 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 696
Psp1406I AACGTT 1 cut(s) 971
Psp5II RGGWCCY 1 cut(s) 696
Psp6I CCWGG 2 cut(s) 393, 401
PspCI CACGTG 1 cut(s) 50
PspGI CCWGG 2 cut(s) 393, 401
PspN4I GGNNCC 6 cut(s) 255, 272, 322, 533, 663, 697
PspPI GGNCC 4 cut(s) 270, 320, 437, 696
PspPPI RGGWCCY 1 cut(s) 696
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 739, 801
PvuI CGATCG 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 4 cut(s) 133, 533, 939, 1013
RsaNI GTAC 4 cut(s) 132, 532, 938, 1012
RseI CAYNNNNRTG 3 cut(s) 52, 126, 470
SaqAI TTAA 3 cut(s) 413, 557, 921
SatI GCNGC 2 cut(s) 240, 426
Sau3AI GATC 7 cut(s) 191, 514, 739, 748, 763, 801, 1004
Sau96I GGNCC 4 cut(s) 270, 320, 437, 696
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 3 cut(s) 395, 403, 666
SduI GDGCHC 2 cut(s) 256, 553
SfaNI GCATC 4 cut(s) 88, 181, 412, 678
SfcI CTRYAG 2 cut(s) 237, 487
SinI GGWCC 3 cut(s) 270, 437, 696
SmiMI CAYNNNNRTG 3 cut(s) 52, 126, 470
SmlI CTYRAG 2 cut(s) 382, 877
SmoI CTYRAG 2 cut(s) 382, 877
SpeI ACTAGT 1 cut(s) 1045
Sse9I AATT 5 cut(s) 309, 554, 772, 914, 978
SspMI CTAG 1 cut(s) 1046
StyD4I CCNGG 3 cut(s) 393, 401, 664
StyI CCWWGG 3 cut(s) 139, 248, 756
TaaI ACNGT 3 cut(s) 491, 937, 1028
TaiI ACGT 4 cut(s) 52, 300, 894, 974
TaqI TCGA 3 cut(s) 190, 649, 674
TasI AATT 5 cut(s) 309, 554, 772, 914, 978
TatI WGTACW 1 cut(s) 1011
TfiI GAWTC 5 cut(s) 58, 73, 344, 496, 827
Tru1I TTAA 3 cut(s) 413, 557, 921
Tru9I TTAA 3 cut(s) 413, 557, 921
TscAI CASTG 4 cut(s) 354, 496, 709, 940
TseI GCWGC 2 cut(s) 239, 425
TspDTI ATGAA 7 cut(s) 29, 101, 302, 451, 612, 804, 900
TspRI CASTG 4 cut(s) 354, 496, 709, 940
VneI GTGCAC 1 cut(s) 549
VpaK11BI GGWCC 3 cut(s) 270, 437, 696
XapI RAATTY 3 cut(s) 309, 772, 914
XmnI GAANNNNTTC 1 cut(s) 72
XspI CTAG 1 cut(s) 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.