Rh7BG194100

Maspardin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012477
Physical Location & Seq
Reverse (-)
15798941 .. 15804986
6046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG194100.1

Sequence Viewer

Length: 1236 bp
ATGAAAGGCGTCTACTCGGCGCCCGGAGATTACATCTACTTCAAGTCCCAGGTCCCTCTTCACAAGATCCCAATTGGCACAAAGCAGTGGCGATACTATGATTTTGGTCCGAAAGCAGTAACTCCACTTATATGTCTTCCTGGAACAGCAGGGACAGCAGATGTCTGTTACAAGCAGATCATGGCATTGTCCATGAAGGGTTACAGGGTCATTTCTGTTGATATTCCACGTGTATGGAATCATCAAGAATGGATTCAAGCATTTGAAAAGTTCTTGGATGCTATTGATGTTCATCATATACATCTCTATGGTACATCCCTTGGGGGCTTTTTGGCTCAACTTTTTGCTCAGCATCGTCCAAGACGGGTTCGATCGTTGATACTATCAAATACCTTTCTGGACACGCGCGACTTCTCTGCAGCAATGCCATGGGCTCCCATCGTTAGTTGGACCCCCTCTTTTTTGCTGAAAAGATACGTCTTATCAGGAATTCGTGATGGCCCCCACGAGCCATTTATTGCAGATTCAGTGGACTTTGTTGTTTCTCAGGTTGAAACACTCTCAAGGGAAGACCTGGCCTCCAGGTTGACTTTAACAGCTGATGCTGCACAAGTTGGACCTCTTTTGCTCACAGACTCATTCATCACCATAATGGATACAAATGACTACAGTGCGATTCCTCAACAACTCAAAGATCAACTGAGTGAAAGGTACCCCGAAGCAAGGCGTGCACAATTAAAGACTGGGGGAGATTTTCCATTTCTTTCACGCCCAGATGAAGTCAACTTACATCTTCAGCTACACCTAAGACGAGTTGGTGTGGAAGCTCGACCTGACTTGGTTCCGGGCATCTCGAAGGGAGGTAGTGGTGGTGGGAGCTCCAATGAAGAGAATGAGAAAAAAGATCAGGACAAAAAAGATCCGGATGATCCATCCAAGGATGATCAGGGAAATTCAGAAAGTTCTAATGAAGAAAGTCAGATCCCACCACCCCCAGAAAGTTCAGAATCTCATAGCTTAGACGACCAGCTTGTCAACAATGCAAAGTTTTGCCCCTTGAGTAATGAAGACGTGGTCGTTTCCTTGCTTTCTGAATTCTTTAAGCAACAACACACTGTACCCCCTGAAATAGTAATGCGATTATGGGAAACGTTTGCTATTCATTTGCTTTCTTTATGTGTGGGATCGTTGTACATTAGTTTGAACTGTAATCAGAAGCTTAGACAAGTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

411

Amino Acids

46.09

Weight (kDa)

5.66

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 42 - 160 3e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 43 - 261 1.6e-08 Alpha/beta hydrolase family
Hydrolase_4 PF12146 84 - 161 4.3e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12230
fragaria_vesca FvH4_5g00640 FvH4_5g00640 FvH4_5g00640
malus_domestica MD04G1079900.v1.1 MD06G1074900.v1.1
prunus_persica Prupe.5G079200_v2.0.a1
pyrus_communis pycom04g07200 pycom06g05350
rosa_chinensis RchiOBHm_Chr7g0201621
rosa_laevigata RLG00000003661
rosa_multiflora Rmu_sc0004811.1_g000001
rosa_roxburghii Rroxscaffold_3G00255190
rosa_rugosa Rorug07G0065200
rosa_samantha Rh7AG191900 Rh7BG194100 Rh7CG203100 Rh7DG197100
rosa_wichuraiana Rw7G016830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1031
Acc65I GGTACC 1 cut(s) 711
AccB1I GGYRCC 2 cut(s) 19, 711
AccI GTMKAC 1 cut(s) 12
AccII CGCG 2 cut(s) 406, 408
AccIII TCCGGA 1 cut(s) 922
AclI AACGTT 1 cut(s) 1151
AclWI GGATC 5 cut(s) 61, 914, 923, 976, 1192
AcsI RAATTY 3 cut(s) 489, 952, 1094
AcuI CTGAAG 1 cut(s) 779
AcvI CACGTG 1 cut(s) 230
AcyI GRCGYC 2 cut(s) 9, 20
AfaI GTAC 4 cut(s) 313, 713, 1119, 1193
AfiI CCNNNNNNNGG 1 cut(s) 723
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 5 cut(s) 43, 257, 266, 554, 1204
AjiI CACGTC 1 cut(s) 1072
AjnI CCWGG 4 cut(s) 48, 139, 573, 581
AluBI AGCT 7 cut(s) 599, 799, 827, 879, 1017, 1030, 1219
AluI AGCT 7 cut(s) 599, 799, 827, 879, 1017, 1030, 1219
Alw21I GWGCWC 2 cut(s) 733, 881
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 5 cut(s) 61, 914, 923, 976, 1192
Aor13HI TCCGGA 1 cut(s) 922
AoxI GGCC 2 cut(s) 499, 576
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 2 cut(s) 419, 605
ApoI RAATTY 3 cut(s) 489, 952, 1094
Asp700I GAANNNNTTC 1 cut(s) 252
Asp718I GGTACC 1 cut(s) 711
AspLEI GCGC 2 cut(s) 22, 408
AspS9I GGNCC 5 cut(s) 52, 107, 450, 500, 617
AsuC2I CCSGG 2 cut(s) 24, 846
AsuHPI GGTGA 1 cut(s) 637
AvaII GGWCC 4 cut(s) 52, 107, 450, 617
BaeGI GKGCMC 1 cut(s) 733
BanI GGYRCC 2 cut(s) 19, 711
BanII GRGCYC 2 cut(s) 436, 881
BarI GAAGNNNNNNTAC 2 cut(s) 771, 803
BauI CACGAG 1 cut(s) 506
BbrPI CACGTG 1 cut(s) 230
BbsI GAAGAC 3 cut(s) 128, 576, 1074
Bbv12I GWGCWC 2 cut(s) 733, 881
BbvI GCAGC 2 cut(s) 431, 592
BccI CCATC 3 cut(s) 446, 491, 940
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BciT130I CCWGG 4 cut(s) 50, 141, 575, 583
BciVI GTATCC 1 cut(s) 649
BclI TGATCA 1 cut(s) 943
BcnI CCSGG 2 cut(s) 24, 846
BfmI CTRYAG 2 cut(s) 417, 667
BfoI RGCGCY 1 cut(s) 23
BfuI GTATCC 1 cut(s) 649
BisI GCNGC 2 cut(s) 420, 606
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 2 cut(s) 421, 607
Bme1390I CCNGG 6 cut(s) 24, 50, 141, 575, 583, 846
Bme18I GGWCC 4 cut(s) 52, 107, 450, 617
BmgBI CACGTC 1 cut(s) 1072
BmgT120I GGNCC 5 cut(s) 52, 107, 450, 500, 617
BmiI GGNNCC 7 cut(s) 21, 54, 435, 452, 502, 713, 843
BmrFI CCNGG 6 cut(s) 24, 50, 141, 575, 583, 846
BmrI ACTGGG 1 cut(s) 753
BmsI GCATC 4 cut(s) 268, 361, 592, 858
BmuI ACTGGG 1 cut(s) 753
BpiI GAAGAC 3 cut(s) 128, 576, 1074
BpmI CTGGAG 1 cut(s) 565
Bpu1102I GCTNAGC 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 547, 1078
BpuMI CCSGG 2 cut(s) 24, 846
BsaAI YACGTR 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 291
BsaHI GRCGYC 2 cut(s) 9, 20
BsaJI CCNNGG 4 cut(s) 48, 319, 428, 936
BsaWI WCCGGW 1 cut(s) 922
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 1 cut(s) 723
Bse1I ACTGG 1 cut(s) 748
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 291
BseAI TCCGGA 1 cut(s) 922
BseBI CCWGG 4 cut(s) 50, 141, 575, 583
BseDI CCNNGG 4 cut(s) 48, 319, 428, 936
BseGI GGATG 5 cut(s) 283, 314, 931, 932, 946
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 723
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 3 cut(s) 362, 560, 692
BseNI ACTGG 1 cut(s) 748
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 2 cut(s) 431, 592
BsgI GTGCAG 1 cut(s) 591
Bsh1236I CGCG 2 cut(s) 406, 408
Bsh1285I CGRYCG 1 cut(s) 374
BshFI GGCC 2 cut(s) 501, 578
BshNI GGYRCC 2 cut(s) 19, 711
BsiEI CGRYCG 1 cut(s) 374
BsiHKAI GWGCWC 2 cut(s) 733, 881
BsiSI CCGG 3 cut(s) 24, 845, 923
BslFI GGGAC 3 cut(s) 31, 38, 166
BslI CCNNNNNNNGG 1 cut(s) 723
BsmFI GGGAC 3 cut(s) 31, 38, 166
BsnI GGCC 2 cut(s) 501, 578
Bsp1286I GDGCHC 3 cut(s) 436, 733, 881
Bsp13I TCCGGA 1 cut(s) 922
Bsp1407I TGTACA 1 cut(s) 1191
Bsp1720I GCTNAGC 1 cut(s) 348
Bsp19I CCATGG 1 cut(s) 428
BspANI GGCC 2 cut(s) 501, 578
BspCNI CTCAG 3 cut(s) 361, 559, 693
BspEI TCCGGA 1 cut(s) 922
BspFNI CGCG 2 cut(s) 406, 408
BspLI GGNNCC 7 cut(s) 21, 54, 435, 452, 502, 713, 843
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 5 cut(s) 61, 914, 923, 976, 1192
BspT107I GGYRCC 2 cut(s) 19, 711
BsrDI GCAATG 1 cut(s) 429
BsrGI TGTACA 1 cut(s) 1191
BsrI ACTGG 1 cut(s) 748
BssECI CCNNGG 4 cut(s) 48, 319, 428, 936
BssNI GRCGYC 2 cut(s) 9, 20
BssSI CACGAG 1 cut(s) 506
BssT1I CCWWGG 3 cut(s) 319, 428, 936
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 4 cut(s) 50, 141, 575, 583
Bst4CI ACNGT 3 cut(s) 671, 1117, 1208
Bst6I CTCTTC 2 cut(s) 63, 882
BstACI GRCGYC 2 cut(s) 9, 20
BstAPI GCANNNNNTGC 1 cut(s) 728
BstAUI TGTACA 1 cut(s) 1191
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 729
BstDEI CTNAG 6 cut(s) 348, 546, 701, 806, 1018, 1220
BstDSI CCRYGG 1 cut(s) 428
BstF5I GGATG 5 cut(s) 283, 314, 931, 932, 946
BstFNI CGCG 2 cut(s) 406, 408
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 2 cut(s) 22, 408
BstMCI CGRYCG 1 cut(s) 374
BstMWI GCNNNNNNNGC 3 cut(s) 155, 605, 728
BstNI CCWGG 4 cut(s) 50, 141, 575, 583
BstSCI CCNGG 6 cut(s) 22, 48, 139, 573, 581, 844
BstSFI CTRYAG 2 cut(s) 417, 667
BstSLI GKGCMC 1 cut(s) 733
BstUI CGCG 2 cut(s) 406, 408
BstV1I GCAGC 2 cut(s) 431, 592
BstV2I GAAGAC 3 cut(s) 128, 576, 1074
BstX2I RGATCY 3 cut(s) 66, 919, 981
BstXI CCANNNNNNTGG 1 cut(s) 234
BstYI RGATCY 3 cut(s) 66, 919, 981
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 2 cut(s) 501, 578
BtgI CCRYGG 1 cut(s) 428
BtrI CACGTC 1 cut(s) 1072
BtsCI GGATG 5 cut(s) 283, 314, 931, 932, 946
BtsI GCAGTG 1 cut(s) 92
BtsIMutI CAGTG 4 cut(s) 92, 534, 676, 1113
Cac8I GCNNGC 1 cut(s) 729
CfoI GCGC 2 cut(s) 22, 408
Cfr13I GGNCC 5 cut(s) 52, 107, 450, 500, 617
Csp6I GTAC 4 cut(s) 312, 712, 1118, 1192
CviAII CATG 3 cut(s) 181, 193, 429
CviQI GTAC 4 cut(s) 312, 712, 1118, 1192
DdeI CTNAG 6 cut(s) 348, 546, 701, 806, 1018, 1220
DinI GGCGCC 1 cut(s) 21
DrdI GACNNNNNNGTC 1 cut(s) 1031
DseDI GACNNNNNNGTC 1 cut(s) 1031
Eam1104I CTCTTC 2 cut(s) 63, 882
EarI CTCTTC 2 cut(s) 63, 882
Ecl136II GAGCTC 1 cut(s) 879
Eco130I CCWWGG 3 cut(s) 319, 428, 936
Eco24I GRGCYC 2 cut(s) 436, 881
Eco47I GGWCC 4 cut(s) 52, 107, 450, 617
Eco53kI GAGCTC 1 cut(s) 879
Eco57I CTGAAG 1 cut(s) 779
Eco72I CACGTG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 879
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 2 cut(s) 489, 1094
EcoRII CCWGG 4 cut(s) 48, 139, 573, 581
EcoT14I CCWWGG 3 cut(s) 319, 428, 936
EcoT38I GRGCYC 2 cut(s) 436, 881
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 3 cut(s) 319, 428, 936
FaeI CATG 3 cut(s) 184, 196, 432
FaqI GGGAC 3 cut(s) 31, 38, 166
FatI CATG 3 cut(s) 180, 192, 428
FbaI TGATCA 1 cut(s) 943
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 420, 606
FokI GGATG 5 cut(s) 290, 301, 919, 938, 953
FriOI GRGCYC 2 cut(s) 436, 881
Fsp4HI GCNGC 2 cut(s) 420, 606
GlaI GCGC 2 cut(s) 21, 407
GluI GCNGC 2 cut(s) 420, 606
GsuI CTGGAG 1 cut(s) 565
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 2 cut(s) 501, 578
HapII CCGG 3 cut(s) 24, 845, 923
HhaI GCGC 2 cut(s) 22, 408
Hin1I GRCGYC 2 cut(s) 9, 20
Hin1II CATG 3 cut(s) 184, 196, 432
Hin6I GCGC 2 cut(s) 20, 406
HinP1I GCGC 2 cut(s) 20, 406
HincII GTYRAC 3 cut(s) 588, 784, 1036
HindII GTYRAC 3 cut(s) 588, 784, 1036
HindIII AAGCTT 1 cut(s) 1217
HinfI GANTC 6 cut(s) 238, 253, 524, 635, 676, 1007
HpaII CCGG 3 cut(s) 24, 845, 923
HphI GGTGA 1 cut(s) 637
Hpy166II GTNNAC 6 cut(s) 13, 532, 588, 731, 784, 1036
Hpy188I TCNGA 6 cut(s) 111, 958, 981, 1006, 1093, 1215
Hpy188III TCNNGA 7 cut(s) 245, 398, 486, 494, 853, 908, 923
Hpy8I GTNNAC 6 cut(s) 13, 532, 588, 731, 784, 1036
HpyAV CCTTC 2 cut(s) 190, 850
HpyCH4III ACNGT 3 cut(s) 671, 1117, 1208
HpyCH4IV ACGT 4 cut(s) 229, 477, 1071, 1151
HpyCH4V TGCA 5 cut(s) 419, 521, 608, 731, 1043
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 605, 728
HpyF3I CTNAG 6 cut(s) 348, 546, 701, 806, 1018, 1220
HpySE526I ACGT 4 cut(s) 229, 477, 1071, 1151
Hsp92I GRCGYC 2 cut(s) 9, 20
Hsp92II CATG 3 cut(s) 184, 196, 432
HspAI GCGC 2 cut(s) 20, 406
KasI GGCGCC 1 cut(s) 19
Kpn2I TCCGGA 1 cut(s) 922
KpnI GGTACC 1 cut(s) 715
Ksp22I TGATCA 1 cut(s) 943
LmnI GCTCC 3 cut(s) 439, 876, 884
Lsp1109I GCAGC 2 cut(s) 431, 592
LweI GCATC 4 cut(s) 268, 361, 592, 858
MaeII ACGT 4 cut(s) 229, 477, 1071, 1151
MaeIII GTNAC 3 cut(s) 118, 167, 200
MboII GAAGA 7 cut(s) 50, 128, 581, 785, 899, 983, 1079
MfeI CAATTG 1 cut(s) 72
MflI RGATCY 3 cut(s) 66, 919, 981
MhlI GDGCHC 3 cut(s) 436, 733, 881
MluCI AATT 5 cut(s) 72, 489, 734, 952, 1094
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 2 cut(s) 428, 595
MnlI CCTC 6 cut(s) 66, 466, 589, 630, 690, 854
MroI TCCGGA 1 cut(s) 922
MroXI GAANNNNTTC 1 cut(s) 252
MseI TTAA 3 cut(s) 593, 737, 1101
MslI CAYNNNNRTG 4 cut(s) 130, 232, 306, 650
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 3 cut(s) 24, 845, 923
MspR9I CCNGG 6 cut(s) 24, 50, 141, 575, 583, 846
MunI CAATTG 1 cut(s) 72
MvaI CCWGG 4 cut(s) 50, 141, 575, 583
MvnI CGCG 2 cut(s) 406, 408
MwoI GCNNNNNNNGC 3 cut(s) 155, 605, 728
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 2 cut(s) 24, 846
NcoI CCATGG 1 cut(s) 428
NlaIII CATG 3 cut(s) 184, 196, 432
NlaIV GGNNCC 7 cut(s) 21, 54, 435, 452, 502, 713, 843
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 5 cut(s) 238, 253, 524, 676, 1007
PflFI GACNNNGTC 1 cut(s) 1073
PfoI TCCNGGA 1 cut(s) 139
PkrI GCNGC 2 cut(s) 421, 607
Ple19I CGATCG 1 cut(s) 374
PleI GAGTC 1 cut(s) 629
PluTI GGCGCC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 230
PmlI CACGTG 1 cut(s) 230
PpsI GAGTC 1 cut(s) 629
Ppu21I YACGTR 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 52
Psp124BI GAGCTC 1 cut(s) 881
Psp1406I AACGTT 1 cut(s) 1151
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 4 cut(s) 48, 139, 573, 581
PspCI CACGTG 1 cut(s) 230
PspGI CCWGG 4 cut(s) 48, 139, 573, 581
PspN4I GGNNCC 7 cut(s) 21, 54, 435, 452, 502, 713, 843
PspPI GGNCC 5 cut(s) 52, 107, 450, 500, 617
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 3 cut(s) 66, 919, 981
PsyI GACNNNGTC 1 cut(s) 1073
PvuI CGATCG 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 4 cut(s) 313, 713, 1119, 1193
RsaNI GTAC 4 cut(s) 312, 712, 1118, 1192
RseI CAYNNNNRTG 4 cut(s) 130, 232, 306, 650
SacI GAGCTC 1 cut(s) 881
SaqAI TTAA 3 cut(s) 593, 737, 1101
SatI GCNGC 2 cut(s) 420, 606
Sau96I GGNCC 5 cut(s) 52, 107, 450, 500, 617
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 6 cut(s) 24, 50, 141, 575, 583, 846
SduI GDGCHC 3 cut(s) 436, 733, 881
SfaNI GCATC 4 cut(s) 268, 361, 592, 858
SfcI CTRYAG 2 cut(s) 417, 667
SfoI GGCGCC 1 cut(s) 21
SinI GGWCC 4 cut(s) 52, 107, 450, 617
SmiMI CAYNNNNRTG 4 cut(s) 130, 232, 306, 650
SmlI CTYRAG 2 cut(s) 562, 1057
SmoI CTYRAG 2 cut(s) 562, 1057
Sse9I AATT 5 cut(s) 72, 489, 734, 952, 1094
SspDI GGCGCC 1 cut(s) 19
SstI GAGCTC 1 cut(s) 881
StyD4I CCNGG 6 cut(s) 22, 48, 139, 573, 581, 844
StyI CCWWGG 3 cut(s) 319, 428, 936
TaaI ACNGT 3 cut(s) 671, 1117, 1208
TaiI ACGT 4 cut(s) 232, 480, 1074, 1154
TaqI TCGA 3 cut(s) 370, 829, 854
TasI AATT 5 cut(s) 72, 489, 734, 952, 1094
TatI WGTACW 1 cut(s) 1191
TfiI GAWTC 5 cut(s) 238, 253, 524, 676, 1007
Tru1I TTAA 3 cut(s) 593, 737, 1101
Tru9I TTAA 3 cut(s) 593, 737, 1101
TscAI CASTG 4 cut(s) 92, 534, 676, 1120
TseI GCWGC 2 cut(s) 419, 605
TspDTI ATGAA 9 cut(s) 17, 209, 281, 631, 792, 900, 984, 1080, 1151
TspRI CASTG 4 cut(s) 92, 534, 676, 1120
Tth111I GACNNNGTC 1 cut(s) 1073
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 4 cut(s) 52, 107, 450, 617
XapI RAATTY 3 cut(s) 489, 952, 1094
XmiI GTMKAC 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.