Prupe.5G079200_v2.0.a1

Maspardin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012477
Physical Location & Seq
Forward (+)
9326686 .. 9332659
5974 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G079200.1

Sequence Viewer

Length: 1239 bp
ATGAAAGGCGTCTACTCGGCGCCCGGAGATTACATCTACTTCAAGTCCCAGGTCCCTCTTCACAAGATCCCAATTGGCACAAAACAGTGGCGATACTATGATTTTGGCCCAAAAGCAGTACCTCCACTTATTTGTCTTCCCGGAACAGCAGGGACAGCTGATGTCTGCTACAAGCAGATCATGTCGTTGTCCATGAAGGGTTATCGGGTGATTTCTGTTGATATTCCACGTGTATGGAATCATCATGAGTGGGTCCAAGCATTTGAGAAATTCTTGGATGCTATCGATGTTCATCATATACATATTTATGGTACATCCCTTGGGGGCTTCTTGGCTCAACTTTTTGCTCAGCATCGTCCAAGACGAGTTCGATCATTGATACTATCAAACACATTTTTGGACACGCGCAGTTTCTCTGCAGCAATGCCATGGGCTCCCATCGTTAGTTGGACCCCTTCTTTTTTGCTGAAAAGATATGTCTTGACCGGAATTCGTGATGGCCCCCATGAGCCATTTATTGCTGATTCGGTGGACTTCGTTGTTTCTCAGGTTGAAACGCTCTCAAGAGAAGACCTGGCCTCCAGGTTAACTTTAACAGCTGATGCTGCCTCAGTTGGACCTCTGTTGCTTACAGACTCATTCATTACTATAATGGATACAAATGACTACAGTGCGATTCCTCAACAACTCAAAGATGAAGTGAGTGAGAGGTACCCTGGTGCAAGGCGAGCACAATTGAAGACGGGGGGAGATTTTCCATTCCTTTCACGGCCAGATGAAGTAAACTTACATCTTCAGCTACATCTAAGACGGGTTGGTGTGGAGGCTCGACCTGATTTGGTTCCAGGCATTCCAAAGGGGGGTAGTGGTGGGAGTTCTAGTGAAGAGAATGAGAAAAAAGATCCAGAAGATCCAGCCAAGGATGAAGATCCAGCCAACGATGATAGGGGAAGCTCAGACAATCCTTTGGCAGAAAGTCAGCTACCTCATGCTCCAGAAAGTTCACAATCTCATAGCTTAGATGACCAGCTTGTCAGCAATGCAATAGTTTGCCATTTCAGTAATGTAGACATGATGGTTTCTTTGCATTTGGGATTCTTGAAGAATCAGCATACTATACCCACTGAAACTGTTATGCGACTTTGTGCGGAAATAATTGCTCTTCAGTTCCTTTCTTTTTATGTGGGATCATTGTACATTAGTTTGAACTGTAATCGGAGGGTCGGACAAGTCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

413

Amino Acids

45.86

Weight (kDa)

6.0

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12230
fragaria_vesca FvH4_5g00640 FvH4_5g00640 FvH4_5g00640
malus_domestica MD04G1079900.v1.1 MD06G1074900.v1.1
prunus_persica Prupe.5G079200_v2.0.a1
pyrus_communis pycom04g07200 pycom06g05350
rosa_chinensis RchiOBHm_Chr7g0201621
rosa_laevigata RLG00000003661
rosa_multiflora Rmu_sc0004811.1_g000001
rosa_roxburghii Rroxscaffold_3G00255190
rosa_rugosa Rorug07G0065200
rosa_samantha Rh7AG191900 Rh7BG194100 Rh7CG203100 Rh7DG197100
rosa_wichuraiana Rw7G016830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1031
Acc65I GGTACC 1 cut(s) 711
AccB1I GGYRCC 2 cut(s) 19, 711
AccI GTMKAC 2 cut(s) 12, 1068
AccII CGCG 1 cut(s) 406
AciI CCGC 1 cut(s) 1148
AclWI GGATC 5 cut(s) 61, 896, 905, 923, 1195
AcoI YGGCCR 1 cut(s) 770
AcsI RAATTY 2 cut(s) 269, 489
AcuI CTGAAG 2 cut(s) 779, 1148
AcvI CACGTG 1 cut(s) 230
AcyI GRCGYC 2 cut(s) 9, 20
AfaI GTAC 4 cut(s) 120, 313, 713, 1196
AfiI CCNNNNNNNGG 1 cut(s) 860
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 5 cut(s) 43, 554, 739, 1102, 1207
AjnI CCWGG 5 cut(s) 48, 573, 581, 715, 844
AluBI AGCT 7 cut(s) 158, 599, 799, 954, 982, 1017, 1030
AluI AGCT 7 cut(s) 158, 599, 799, 954, 982, 1017, 1030
Alw21I GWGCWC 1 cut(s) 733
AlwI GGATC 5 cut(s) 61, 896, 905, 923, 1195
AoxI GGCC 4 cut(s) 106, 499, 576, 770
ApeKI GCWGC 2 cut(s) 419, 605
ApoI RAATTY 2 cut(s) 269, 489
Asp718I GGTACC 1 cut(s) 711
AspLEI GCGC 2 cut(s) 22, 408
AspS9I GGNCC 6 cut(s) 52, 107, 253, 450, 500, 617
AsuC2I CCSGG 2 cut(s) 24, 141
AsuHPI GGTGA 1 cut(s) 220
AvaII GGWCC 4 cut(s) 52, 253, 450, 617
BaeI ACNNNNGTAYC 2 cut(s) 703, 736
BanI GGYRCC 2 cut(s) 19, 711
BanII GRGCYC 1 cut(s) 436
BarI GAAGNNNNNNTAC 2 cut(s) 771, 803
BbrPI CACGTG 1 cut(s) 230
BbsI GAAGAC 3 cut(s) 128, 576, 746
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 2 cut(s) 431, 592
BccI CCATC 3 cut(s) 446, 491, 1069
BceAI ACGGC 1 cut(s) 785
BciT130I CCWGG 5 cut(s) 50, 575, 583, 717, 846
BciVI GTATCC 1 cut(s) 649
BcnI CCSGG 2 cut(s) 24, 141
BfaI CTAG 1 cut(s) 879
BfmI CTRYAG 2 cut(s) 417, 667
BfoI RGCGCY 1 cut(s) 23
BfuI GTATCC 1 cut(s) 649
BisI GCNGC 2 cut(s) 420, 606
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 2 cut(s) 421, 607
Bme1390I CCNGG 7 cut(s) 24, 50, 141, 575, 583, 717, 846
Bme18I GGWCC 4 cut(s) 52, 253, 450, 617
BmgT120I GGNCC 6 cut(s) 52, 107, 253, 450, 500, 617
BmiI GGNNCC 8 cut(s) 21, 54, 254, 435, 452, 502, 713, 843
BmrFI CCNGG 7 cut(s) 24, 50, 141, 575, 583, 717, 846
BmsI GCATC 3 cut(s) 268, 361, 592
BpiI GAAGAC 3 cut(s) 128, 576, 746
BpmI CTGGAG 2 cut(s) 565, 978
Bpu1102I GCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 547
BpuMI CCSGG 2 cut(s) 24, 141
Bsa29I ATCGAT 1 cut(s) 285
BsaAI YACGTR 1 cut(s) 230
BsaBI GATNNNNATC 2 cut(s) 291, 927
BsaHI GRCGYC 2 cut(s) 9, 20
BsaJI CCNNGG 5 cut(s) 48, 319, 428, 715, 918
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 4 cut(s) 563, 593, 865, 895
Bsc4I CCNNNNNNNGG 1 cut(s) 860
Bse3DI GCAATG 2 cut(s) 429, 1045
Bse8I GATNNNNATC 2 cut(s) 291, 927
BseBI CCWGG 5 cut(s) 50, 575, 583, 717, 846
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 5 cut(s) 48, 319, 428, 715, 918
BseGI GGATG 3 cut(s) 283, 314, 928
BseJI GATNNNNATC 2 cut(s) 291, 927
BseLI CCNNNNNNNGG 1 cut(s) 860
BseMI GCAATG 2 cut(s) 429, 1045
BseMII CTCAG 4 cut(s) 362, 560, 624, 969
BseXI GCAGC 2 cut(s) 431, 592
Bsh1236I CGCG 1 cut(s) 406
BshFI GGCC 4 cut(s) 108, 501, 578, 772
BshNI GGYRCC 2 cut(s) 19, 711
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 733
BsiSI CCGG 3 cut(s) 24, 141, 486
BslFI GGGAC 3 cut(s) 31, 38, 166
BslI CCNNNNNNNGG 1 cut(s) 860
BsmFI GGGAC 3 cut(s) 31, 38, 166
BsmI GAATGC 1 cut(s) 849
BsnI GGCC 4 cut(s) 108, 501, 578, 772
Bsp1286I GDGCHC 2 cut(s) 436, 733
Bsp1407I TGTACA 1 cut(s) 1194
Bsp143I GATC 7 cut(s) 66, 177, 371, 901, 910, 928, 1187
Bsp1720I GCTNAGC 1 cut(s) 348
Bsp19I CCATGG 1 cut(s) 428
BspACI CCGC 1 cut(s) 1148
BspANI GGCC 4 cut(s) 108, 501, 578, 772
BspCNI CTCAG 4 cut(s) 361, 559, 623, 968
BspDI ATCGAT 1 cut(s) 285
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 8 cut(s) 21, 54, 254, 435, 452, 502, 713, 843
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 5 cut(s) 61, 896, 905, 923, 1195
BspQI GCTCTTC 1 cut(s) 1167
BspT107I GGYRCC 2 cut(s) 19, 711
BsrDI GCAATG 2 cut(s) 429, 1045
BsrGI TGTACA 1 cut(s) 1194
BssECI CCNNGG 5 cut(s) 48, 319, 428, 715, 918
BssMI GATC 7 cut(s) 66, 177, 371, 901, 910, 928, 1187
BssNI GRCGYC 2 cut(s) 9, 20
BssT1I CCWWGG 3 cut(s) 319, 428, 918
Bst2UI CCWGG 5 cut(s) 50, 575, 583, 717, 846
Bst4CI ACNGT 4 cut(s) 87, 671, 1132, 1211
Bst6I CTCTTC 3 cut(s) 63, 879, 1167
BstACI GRCGYC 2 cut(s) 9, 20
BstAUI TGTACA 1 cut(s) 1194
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 729
BstDEI CTNAG 6 cut(s) 348, 546, 610, 806, 955, 1018
BstDSI CCRYGG 1 cut(s) 428
BstF5I GGATG 3 cut(s) 283, 314, 928
BstFNI CGCG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 2 cut(s) 22, 408
BstKTI GATC 7 cut(s) 69, 180, 374, 904, 913, 931, 1190
BstMBI GATC 7 cut(s) 66, 177, 371, 901, 910, 928, 1187
BstMWI GCNNNNNNNGC 3 cut(s) 155, 605, 728
BstNI CCWGG 5 cut(s) 50, 575, 583, 717, 846
BstSCI CCNGG 7 cut(s) 22, 48, 139, 573, 581, 715, 844
BstSFI CTRYAG 2 cut(s) 417, 667
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 2 cut(s) 431, 592
BstV2I GAAGAC 3 cut(s) 128, 576, 746
BstX2I RGATCY 4 cut(s) 66, 901, 910, 928
BstXI CCANNNNNNTGG 1 cut(s) 234
BstYI RGATCY 4 cut(s) 66, 901, 910, 928
Bsu15I ATCGAT 1 cut(s) 285
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 4 cut(s) 108, 501, 578, 772
BsuTUI ATCGAT 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 428
BtsCI GGATG 3 cut(s) 283, 314, 928
BtsIMutI CAGTG 3 cut(s) 92, 676, 1122
Cac8I GCNNGC 1 cut(s) 729
CciI TCATGA 1 cut(s) 244
CfoI GCGC 2 cut(s) 22, 408
Cfr13I GGNCC 6 cut(s) 52, 107, 253, 450, 500, 617
ClaI ATCGAT 1 cut(s) 285
Csp6I GTAC 4 cut(s) 119, 312, 712, 1195
CviAII CATG 7 cut(s) 181, 193, 245, 429, 506, 989, 1072
CviQI GTAC 4 cut(s) 119, 312, 712, 1195
DdeI CTNAG 6 cut(s) 348, 546, 610, 806, 955, 1018
DinI GGCGCC 1 cut(s) 21
DpnI GATC 7 cut(s) 68, 179, 373, 903, 912, 930, 1189
DpnII GATC 7 cut(s) 66, 177, 371, 901, 910, 928, 1187
DrdI GACNNNNNNGTC 1 cut(s) 1031
DseDI GACNNNNNNGTC 1 cut(s) 1031
EaeI YGGCCR 1 cut(s) 770
Eam1104I CTCTTC 3 cut(s) 63, 879, 1167
EarI CTCTTC 3 cut(s) 63, 879, 1167
Eco130I CCWWGG 3 cut(s) 319, 428, 918
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 4 cut(s) 52, 253, 450, 617
Eco57I CTGAAG 2 cut(s) 779, 1148
Eco72I CACGTG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 5 cut(s) 48, 573, 581, 715, 844
EcoT14I CCWWGG 3 cut(s) 319, 428, 918
EcoT38I GRGCYC 1 cut(s) 436
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 3 cut(s) 319, 428, 918
FaeI CATG 7 cut(s) 184, 196, 248, 432, 509, 992, 1075
FaqI GGGAC 3 cut(s) 31, 38, 166
FatI CATG 7 cut(s) 180, 192, 244, 428, 505, 988, 1071
FblI GTMKAC 2 cut(s) 12, 1068
Fnu4HI GCNGC 2 cut(s) 420, 606
FokI GGATG 3 cut(s) 290, 301, 935
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 2 cut(s) 420, 606
FspBI CTAG 1 cut(s) 879
GlaI GCGC 2 cut(s) 21, 407
GluI GCNGC 2 cut(s) 420, 606
GsuI CTGGAG 2 cut(s) 565, 978
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 4 cut(s) 108, 501, 578, 772
HapII CCGG 3 cut(s) 24, 141, 486
HhaI GCGC 2 cut(s) 22, 408
Hin1I GRCGYC 2 cut(s) 9, 20
Hin1II CATG 7 cut(s) 184, 196, 248, 432, 509, 992, 1075
Hin6I GCGC 2 cut(s) 20, 406
HinP1I GCGC 2 cut(s) 20, 406
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HinfI GANTC 6 cut(s) 238, 524, 635, 676, 1095, 1105
HpaI GTTAAC 1 cut(s) 588
HpaII CCGG 3 cut(s) 24, 141, 486
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 6 cut(s) 13, 532, 588, 784, 1004, 1069
Hpy188I TCNGA 3 cut(s) 958, 1218, 1226
Hpy188III TCNNGA 7 cut(s) 245, 481, 494, 564, 905, 995, 1099
Hpy8I GTNNAC 6 cut(s) 13, 532, 588, 784, 1004, 1069
HpyAV CCTTC 2 cut(s) 190, 465
HpyCH4III ACNGT 4 cut(s) 87, 671, 1132, 1211
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 4 cut(s) 419, 722, 1043, 1087
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 605, 728
HpyF3I CTNAG 6 cut(s) 348, 546, 610, 806, 955, 1018
HpySE526I ACGT 1 cut(s) 229
Hsp92I GRCGYC 2 cut(s) 9, 20
Hsp92II CATG 7 cut(s) 184, 196, 248, 432, 509, 992, 1075
HspAI GCGC 2 cut(s) 20, 406
KasI GGCGCC 1 cut(s) 19
KpnI GGTACC 1 cut(s) 715
KspAI GTTAAC 1 cut(s) 588
Kzo9I GATC 7 cut(s) 66, 177, 371, 901, 910, 928, 1187
LguI GCTCTTC 1 cut(s) 1167
LmnI GCTCC 2 cut(s) 439, 997
Lsp1109I GCAGC 2 cut(s) 431, 592
LweI GCATC 3 cut(s) 268, 361, 592
MaeI CTAG 1 cut(s) 879
MaeII ACGT 1 cut(s) 229
MalI GATC 7 cut(s) 68, 179, 373, 903, 912, 930, 1189
MboI GATC 7 cut(s) 66, 177, 371, 901, 910, 928, 1187
MfeI CAATTG 2 cut(s) 72, 734
MflI RGATCY 4 cut(s) 66, 901, 910, 928
MhlI GDGCHC 2 cut(s) 436, 733
MluCI AATT 5 cut(s) 72, 269, 489, 734, 1155
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 3 cut(s) 428, 595, 1204
MseI TTAA 2 cut(s) 587, 593
MslI CAYNNNNRTG 2 cut(s) 232, 306
MspA1I CMGCKG 2 cut(s) 158, 599
MspI CCGG 3 cut(s) 24, 141, 486
MspR9I CCNGG 7 cut(s) 24, 50, 141, 575, 583, 717, 846
MunI CAATTG 2 cut(s) 72, 734
Mva1269I GAATGC 1 cut(s) 849
MvaI CCWGG 5 cut(s) 50, 575, 583, 717, 846
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 3 cut(s) 155, 605, 728
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 2 cut(s) 24, 141
NcoI CCATGG 1 cut(s) 428
NdeII GATC 7 cut(s) 66, 177, 371, 901, 910, 928, 1187
NlaIII CATG 7 cut(s) 184, 196, 248, 432, 509, 992, 1075
NlaIV GGNNCC 8 cut(s) 21, 54, 254, 435, 452, 502, 713, 843
PagI TCATGA 1 cut(s) 244
PciSI GCTCTTC 1 cut(s) 1167
PcsI WCGNNNNNNNCGW 2 cut(s) 361, 1230
PctI GAATGC 1 cut(s) 849
PfeI GAWTC 5 cut(s) 238, 524, 676, 1095, 1105
PfoI TCCNGGA 1 cut(s) 139
PkrI GCNGC 2 cut(s) 421, 607
PleI GAGTC 1 cut(s) 629
PluTI GGCGCC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 230
PmlI CACGTG 1 cut(s) 230
PpsI GAGTC 1 cut(s) 629
Ppu21I YACGTR 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 5 cut(s) 48, 573, 581, 715, 844
PspCI CACGTG 1 cut(s) 230
PspGI CCWGG 5 cut(s) 48, 573, 581, 715, 844
PspN4I GGNNCC 8 cut(s) 21, 54, 254, 435, 452, 502, 713, 843
PspPI GGNCC 6 cut(s) 52, 107, 253, 450, 500, 617
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 4 cut(s) 66, 901, 910, 928
PvuII CAGCTG 2 cut(s) 158, 599
RsaI GTAC 4 cut(s) 120, 313, 713, 1196
RsaNI GTAC 4 cut(s) 119, 312, 712, 1195
RseI CAYNNNNRTG 2 cut(s) 232, 306
SapI GCTCTTC 1 cut(s) 1167
SaqAI TTAA 2 cut(s) 587, 593
SatI GCNGC 2 cut(s) 420, 606
Sau3AI GATC 7 cut(s) 66, 177, 371, 901, 910, 928, 1187
Sau96I GGNCC 6 cut(s) 52, 107, 253, 450, 500, 617
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 7 cut(s) 24, 50, 141, 575, 583, 717, 846
SduI GDGCHC 2 cut(s) 436, 733
SfaNI GCATC 3 cut(s) 268, 361, 592
SfcI CTRYAG 2 cut(s) 417, 667
SfoI GGCGCC 1 cut(s) 21
SinI GGWCC 4 cut(s) 52, 253, 450, 617
SmiMI CAYNNNNRTG 2 cut(s) 232, 306
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 5 cut(s) 72, 269, 489, 734, 1155
SsiI CCGC 1 cut(s) 1148
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 1 cut(s) 879
StyD4I CCNGG 7 cut(s) 22, 48, 139, 573, 581, 715, 844
StyI CCWWGG 3 cut(s) 319, 428, 918
TaaI ACNGT 4 cut(s) 87, 671, 1132, 1211
TaiI ACGT 1 cut(s) 232
TaqI TCGA 3 cut(s) 285, 370, 829
TasI AATT 5 cut(s) 72, 269, 489, 734, 1155
TatI WGTACW 1 cut(s) 1194
TfiI GAWTC 5 cut(s) 238, 524, 676, 1095, 1105
Tru1I TTAA 2 cut(s) 587, 593
Tru9I TTAA 2 cut(s) 587, 593
TscAI CASTG 3 cut(s) 92, 676, 1129
TseI GCWGC 2 cut(s) 419, 605
TspDTI ATGAA 7 cut(s) 17, 209, 281, 631, 711, 792, 939
TspRI CASTG 3 cut(s) 92, 676, 1129
VpaK11BI GGWCC 4 cut(s) 52, 253, 450, 617
XapI RAATTY 2 cut(s) 269, 489
XmiI GTMKAC 2 cut(s) 12, 1068
XspI CTAG 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.