AT4G12400
HSP70 Family

hsp70-Hsp90 organizing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011330
Physical Location & Seq
Reverse (-)
7338018 .. 7341363
3346 bp
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UTR
Exon/CDS
Intron
AT4G12400.1

Sequence Viewer

Length: 1593 bp
ATGGCGGAAGAAGCAAAATCCAAAGGAAACGCAGCTTTCTCTTCCGGCGATTACGCCACCGCAATAACCCATTTCACAGAAGCAATCAACCTTTCACCAACCAATCACATCCTCTACTCAAACAGATCCGCTTCTTACGCTTCTCTCCACCGTTACGAAGAAGCTTTATCAGACGCGAAGAAGACTATAGAGCTTAAACCTGATTGGTCTAAAGGATATAGCCGATTAGGTGCTGCGTTTATTGGATTGTCCAAGTTTGATGAAGCGGTTGATTCGTATAAGAAAGGATTAGAGATTGATCCGAGTAATGAGATGCTTAAATCGGGATTAGCTGATGCTTCGAGATCTAGGGTTTCGTCAAAGTCGAATCCTTTTGTTGATGCGTTTCAAGGGAAGGAGATGTGGGAGAAGTTGACGGCGGATCCGGGGACTAGGGTTTATTTGGAGCAGGATGATTTTGTTAAGACGATGAAGGAGATTCAGAGGAACCCTAATAATCTTAATTTGTATATGAAGGATAAGAGAGTTATGAAGGCTTTAGGGGTTTTGTTGAATGTGAAGTTTGGTGGATCTAGTGGTGAAGATACTGAGATGAAGGAGGCTGATGAGAGGAAAGAGCCTGAACCGGAGATGGAACCTATGGAGTTGACGGAGGAGGAGAGGCAGAAGAAGGAGAGAAAGGAGAAGGCTTTGAAGGAGAAAGGGGAAGGAAATGTTGCTTATAAGAAGAAGGATTTTGGGAGAGCTGTTGAACATTATACTAAGGCCATGGAGCTCGATGATGAGGATATTTCGTATTTGACGAATCGTGCTGCTGTTTATCTTGAGATGGGGAAGTACGAGGAGTGCATTGAAGACTGTGACAAGGCTGTTGAAAGAGGCAGAGAACTTCGTTCTGACTTCAAGATGATAGCAAGAGCTCTGACTAGAAAAGGATCTGCTCTAGTGAAAATGGCGAGATGCTCGAAAGACTTTGAGCCTGCGATTGAGACTTTCCAGAAAGCTCTTACAGAGCATCGTAATCCAGATACATTGAAGAAACTGAACGATGCTGAGAAAGTCAAGAAAGAGCTGGAGCAACAGGAGTACTTTGATCCTACGATAGCCGAGGAGGAGCGAGAGAAAGGTAATGGATTCTTTAAAGAACAAAAGTATCCAGAGGCAGTGAAGCATTATTCAGAAGCAATCAAAAGAAACCCGAACGACGTGAGGGCATATAGCAACAGAGCTGCTTGTTACACAAAGTTAGGAGCATTACCAGAGGGATTGAAAGATGCTGAAAAATGCATTGAGCTGGACCCAAGTTTCACGAAAGGATACAGTAGAAAAGGAGCTATTCAATTTTTCATGAAGGAATACGATAAAGCTATGGAAACGTATCAAGAAGGGCTAAAACATGATCCTAAGAACCAGGAGTTCCTTGATGGTGTTAGAAGATGTGTGGAACAGATAAACAAAGCGAGCCGTGGTGATCTGACTCCAGAAGAATTGAAGGAGAGACAAGCAAAGGCAATGCAAGATCCTGAAGTTCAGAACATATTATCGGATCCAGTGATGAGACAGGTAAAAGCAGTGGCAAGCATTGTGTTCTAA

Protein Analysis

530

Amino Acids

60.45

Weight (kDa)

5.84

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 4 - 71 1.3e-08 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_11 PF13414 9 - 47 1e-05 TPR repeat
TPR_1 PF00515 71 - 103 2.3e-07 Tetratricopeptide repeat
STI1-HOP_DP PF17830 132 - 184 3e-17 STI1/HOP, DP domain
TPR_BSK1_C PF25575 225 - 294 1e-06 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 267 - 292 1.3e-06 Tetratricopeptide repeat
TPR_12 PF13424 267 - 336 1.3e-06 Tetratricopeptide repeat
TPR_16 PF13432 375 - 427 1e-05 Tetratricopeptide repeat
TPR_11 PF13414 376 - 417 2.1e-09 TPR repeat
TPR_1 PF00515 404 - 436 1.2e-06 Tetratricopeptide repeat
TPR_1 PF00515 438 - 470 1.2e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62740 AT4G12400 AT4G12400
fragaria_vesca FvH4_5g00400 FvH4_5g00400 FvH4_5g00400
malus_domestica MD04G1081900.v1.1 MD06G1065400.v1.1
prunus_persica Prupe.5G076500_v2.0.a1
pyrus_communis pycom04g07380 pycom06g05640
rosa_chinensis RchiOBHm_Chr7g0202131
rosa_laevigata RLG00000003623
rosa_multiflora Rmu_ssc0000228.1_g000003
rosa_roxburghii Rroxscaffold_3G00254830
rosa_rugosa Rorug07G0068200 Rorug07G0068300
rosa_samantha Rh7AG195700 Rh7CG206800
rosa_wichuraiana Rw7G017140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
AccII CGCG 1 cut(s) 176
AciI CCGC 5 cut(s) 5, 60, 129, 266, 419
AcuI CTGAAG 1 cut(s) 1545
AfaI GTAC 2 cut(s) 839, 1088
AjiI CACGTC 1 cut(s) 1207
AjnI CCWGG 1 cut(s) 1410
Alw21I GWGCWC 2 cut(s) 777, 922
Alw26I GTCTC 3 cut(s) 983, 1492, 1552
AoxI GGCC 1 cut(s) 765
ApeKI GCWGC 4 cut(s) 32, 233, 812, 1229
AspS9I GGNCC 1 cut(s) 1297
AsuC2I CCSGG 1 cut(s) 426
AsuHPI GGTGA 3 cut(s) 87, 590, 1481
AvaII GGWCC 1 cut(s) 1297
BaeI ACNNNNGTAYC 2 cut(s) 1136, 1169
BamHI GGATCC 2 cut(s) 421, 1546
BanII GRGCYC 2 cut(s) 777, 922
BbsI GAAGAC 2 cut(s) 188, 861
Bbv12I GWGCWC 2 cut(s) 777, 922
BbvI GCAGC 4 cut(s) 44, 220, 799, 1216
BccI CCATC 3 cut(s) 625, 823, 1418
BceAI ACGGC 2 cut(s) 432, 1449
BciT130I CCWGG 1 cut(s) 1412
BciVI GTATCC 2 cut(s) 1164, 1310
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 3 cut(s) 983, 1492, 1552
BfaI CTAG 5 cut(s) 348, 432, 573, 927, 944
BfmI CTRYAG 1 cut(s) 186
BfuI GTATCC 2 cut(s) 1164, 1310
BglII AGATCT 1 cut(s) 344
BisI GCNGC 4 cut(s) 33, 234, 813, 1230
BlsI GCNGC 4 cut(s) 34, 235, 814, 1231
BmcAI AGTACT 1 cut(s) 1088
Bme1390I CCNGG 2 cut(s) 426, 1412
Bme18I GGWCC 1 cut(s) 1297
BmgBI CACGTC 1 cut(s) 1207
BmgT120I GGNCC 1 cut(s) 1297
BmiI GGNNCC 5 cut(s) 423, 488, 636, 1299, 1548
BmrFI CCNGG 2 cut(s) 426, 1412
BmsI GCATC 7 cut(s) 303, 325, 370, 950, 1024, 1039, 1264
BpiI GAAGAC 2 cut(s) 188, 861
BpmI CTGGAG 2 cut(s) 1094, 1464
BpuEI CTTGAG 1 cut(s) 845
BpuMI CCSGG 1 cut(s) 426
BsaJI CCNNGG 4 cut(s) 425, 768, 1107, 1465
BsaWI WCCGGW 1 cut(s) 625
Bse1I ACTGG 1 cut(s) 1550
Bse3DI GCAATG 1 cut(s) 1518
BseBI CCWGG 1 cut(s) 1412
BseDI CCNNGG 4 cut(s) 425, 768, 1107, 1465
BseGI GGATG 2 cut(s) 108, 457
BseMI GCAATG 1 cut(s) 1518
BseMII CTCAG 2 cut(s) 579, 1044
BseNI ACTGG 1 cut(s) 1550
BseRI GAGGAG 5 cut(s) 668, 671, 857, 1124, 1127
BseXI GCAGC 4 cut(s) 44, 220, 799, 1216
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 1 cut(s) 767
BsiHKAI GWGCWC 2 cut(s) 777, 922
BsiSI CCGG 3 cut(s) 45, 425, 626
BslFI GGGAC 1 cut(s) 442
BsmAI GTCTC 3 cut(s) 983, 1492, 1552
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 777, 922
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 5 cut(s) 5, 60, 129, 266, 419
BspANI GGCC 1 cut(s) 767
BspCNI CTCAG 2 cut(s) 580, 1045
BspFNI CGCG 1 cut(s) 176
BspHI TCATGA 1 cut(s) 1347
BspLI GGNNCC 5 cut(s) 423, 488, 636, 1299, 1548
BsrDI GCAATG 1 cut(s) 1518
BsrI ACTGG 1 cut(s) 1550
BssECI CCNNGG 4 cut(s) 425, 768, 1107, 1465
BssT1I CCWWGG 1 cut(s) 768
Bst2UI CCWGG 1 cut(s) 1412
Bst4CI ACNGT 3 cut(s) 152, 860, 1322
Bst6I CTCTTC 1 cut(s) 46
BstC8I GCNNGC 3 cut(s) 981, 1462, 1579
BstDEI CTNAG 4 cut(s) 588, 762, 1053, 1404
BstDSI CCRYGG 2 cut(s) 768, 1465
BstF5I GGATG 2 cut(s) 108, 457
BstFNI CGCG 1 cut(s) 176
BstMAI GTCTC 3 cut(s) 983, 1492, 1552
BstMWI GCNNNNNNNGC 2 cut(s) 11, 137
BstNI CCWGG 1 cut(s) 1412
BstSCI CCNGG 2 cut(s) 424, 1410
BstSFI CTRYAG 1 cut(s) 186
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 4 cut(s) 44, 220, 799, 1216
BstV2I GAAGAC 2 cut(s) 188, 861
BstX2I RGATCY 7 cut(s) 125, 344, 421, 569, 935, 1519, 1546
BstYI RGATCY 7 cut(s) 125, 344, 421, 569, 935, 1519, 1546
BsuI GTATCC 2 cut(s) 1164, 1310
BsuRI GGCC 1 cut(s) 767
BtgI CCRYGG 2 cut(s) 768, 1465
BtrI CACGTC 1 cut(s) 1207
BtsCI GGATG 2 cut(s) 108, 457
BtsI GCAGTG 2 cut(s) 1170, 1578
BtsIMutI CAGTG 3 cut(s) 1170, 1557, 1578
Cac8I GCNNGC 3 cut(s) 981, 1462, 1579
CciI TCATGA 1 cut(s) 1347
Cfr13I GGNCC 1 cut(s) 1297
CseI GACGC 1 cut(s) 182
Csp6I GTAC 2 cut(s) 838, 1087
CviAII CATG 3 cut(s) 769, 1348, 1397
CviQI GTAC 2 cut(s) 838, 1087
DdeI CTNAG 4 cut(s) 588, 762, 1053, 1404
DraI TTTAAA 1 cut(s) 1141
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
EciI GGCGGA 2 cut(s) 20, 434
Ecl136II GAGCTC 2 cut(s) 775, 920
Eco130I CCWWGG 1 cut(s) 768
Eco24I GRGCYC 2 cut(s) 777, 922
Eco47I GGWCC 1 cut(s) 1297
Eco53kI GAGCTC 2 cut(s) 775, 920
Eco57I CTGAAG 1 cut(s) 1545
EcoICRI GAGCTC 2 cut(s) 775, 920
EcoRII CCWGG 1 cut(s) 1410
EcoT14I CCWWGG 1 cut(s) 768
EcoT22I ATGCAT 1 cut(s) 1289
EcoT38I GRGCYC 2 cut(s) 777, 922
ErhI CCWWGG 1 cut(s) 768
FaeI CATG 3 cut(s) 772, 1351, 1400
FaqI GGGAC 1 cut(s) 442
FatI CATG 3 cut(s) 768, 1347, 1396
Fnu4HI GCNGC 4 cut(s) 33, 234, 813, 1230
FokI GGATG 2 cut(s) 95, 464
FriOI GRGCYC 2 cut(s) 777, 922
Fsp4HI GCNGC 4 cut(s) 33, 234, 813, 1230
FspBI CTAG 5 cut(s) 348, 432, 573, 927, 944
GluI GCNGC 4 cut(s) 33, 234, 813, 1230
GsuI CTGGAG 2 cut(s) 1094, 1464
HaeIII GGCC 1 cut(s) 767
HapII CCGG 3 cut(s) 45, 425, 626
HgaI GACGC 1 cut(s) 182
Hin1II CATG 3 cut(s) 772, 1351, 1400
HincII GTYRAC 2 cut(s) 414, 648
HindII GTYRAC 2 cut(s) 414, 648
HindIII AAGCTT 1 cut(s) 162
HinfI GANTC 6 cut(s) 272, 367, 478, 805, 1134, 1477
HpaII CCGG 3 cut(s) 45, 425, 626
HphI GGTGA 3 cut(s) 87, 590, 1481
Hpy166II GTNNAC 2 cut(s) 414, 648
Hpy188I TCNGA 9 cut(s) 172, 303, 483, 898, 924, 1180, 1476, 1533, 1546
Hpy8I GTNNAC 2 cut(s) 414, 648
Hpy99I CGWCG 1 cut(s) 1208
HpyCH4III ACNGT 3 cut(s) 152, 860, 1322
HpyCH4IV ACGT 2 cut(s) 1206, 1376
HpyCH4V TGCA 3 cut(s) 849, 1287, 1516
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 137
HpyF3I CTNAG 4 cut(s) 588, 762, 1053, 1404
HpySE526I ACGT 2 cut(s) 1206, 1376
Hsp92II CATG 3 cut(s) 772, 1351, 1400
LmnI GCTCC 6 cut(s) 445, 772, 1075, 1114, 1250, 1331
Lsp1109I GCAGC 4 cut(s) 44, 220, 799, 1216
LweI GCATC 7 cut(s) 303, 325, 370, 950, 1024, 1039, 1264
MaeI CTAG 5 cut(s) 348, 432, 573, 927, 944
MaeII ACGT 2 cut(s) 1206, 1376
MaeIII GTNAC 3 cut(s) 152, 860, 1235
MflI RGATCY 7 cut(s) 125, 344, 421, 569, 935, 1519, 1546
MhlI GDGCHC 2 cut(s) 777, 922
MluCI AATT 3 cut(s) 502, 1340, 1487
MlyI GAGTC 1 cut(s) 1471
Mph1103I ATGCAT 1 cut(s) 1289
MseI TTAA 5 cut(s) 195, 318, 462, 501, 1140
MspI CCGG 3 cut(s) 45, 425, 626
MspR9I CCNGG 2 cut(s) 426, 1412
MvaI CCWGG 1 cut(s) 1412
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 11, 137
NciI CCSGG 1 cut(s) 426
NcoI CCATGG 1 cut(s) 768
NlaIII CATG 3 cut(s) 772, 1351, 1400
NlaIV GGNNCC 5 cut(s) 423, 488, 636, 1299, 1548
NmeAIII GCCGAG 1 cut(s) 1132
NmuCI GTSAC 1 cut(s) 860
NsiI ATGCAT 1 cut(s) 1289
PagI TCATGA 1 cut(s) 1347
PcsI WCGNNNNNNNCGW 2 cut(s) 362, 800
PfeI GAWTC 5 cut(s) 272, 367, 478, 805, 1134
PkrI GCNGC 4 cut(s) 34, 235, 814, 1231
PleI GAGTC 1 cut(s) 1471
PpsI GAGTC 1 cut(s) 1471
PsiI TTATAA 1 cut(s) 723
Psp124BI GAGCTC 2 cut(s) 777, 922
Psp6I CCWGG 1 cut(s) 1410
PspGI CCWGG 1 cut(s) 1410
PspN4I GGNNCC 5 cut(s) 423, 488, 636, 1299, 1548
PspPI GGNCC 1 cut(s) 1297
PsuI RGATCY 7 cut(s) 125, 344, 421, 569, 935, 1519, 1546
RsaI GTAC 2 cut(s) 839, 1088
RsaNI GTAC 2 cut(s) 838, 1087
SacI GAGCTC 2 cut(s) 777, 922
SaqAI TTAA 5 cut(s) 195, 318, 462, 501, 1140
SatI GCNGC 4 cut(s) 33, 234, 813, 1230
Sau96I GGNCC 1 cut(s) 1297
ScaI AGTACT 1 cut(s) 1088
SchI GAGTC 1 cut(s) 1471
ScrFI CCNGG 2 cut(s) 426, 1412
SduI GDGCHC 2 cut(s) 777, 922
SfaNI GCATC 7 cut(s) 303, 325, 370, 950, 1024, 1039, 1264
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 1 cut(s) 1297
SmlI CTYRAG 1 cut(s) 824
SmoI CTYRAG 1 cut(s) 824
Sse9I AATT 3 cut(s) 502, 1340, 1487
SsiI CCGC 5 cut(s) 5, 60, 129, 266, 419
SspMI CTAG 5 cut(s) 348, 432, 573, 927, 944
SstI GAGCTC 2 cut(s) 777, 922
StyD4I CCNGG 2 cut(s) 424, 1410
StyI CCWWGG 1 cut(s) 768
TaaI ACNGT 3 cut(s) 152, 860, 1322
TaiI ACGT 2 cut(s) 1209, 1379
TaqI TCGA 4 cut(s) 341, 365, 777, 965
TasI AATT 3 cut(s) 502, 1340, 1487
TatI WGTACW 1 cut(s) 1086
TfiI GAWTC 5 cut(s) 272, 367, 478, 805, 1134
Tru1I TTAA 5 cut(s) 195, 318, 462, 501, 1140
Tru9I TTAA 5 cut(s) 195, 318, 462, 501, 1140
TscAI CASTG 3 cut(s) 1170, 1557, 1578
TseFI GTSAC 1 cut(s) 860
TseI GCWGC 4 cut(s) 32, 233, 812, 1229
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 7 cut(s) 276, 485, 527, 545, 608, 1336, 1364
TspGWI ACGGA 1 cut(s) 665
TspRI CASTG 3 cut(s) 1170, 1557, 1578
VpaK11BI GGWCC 1 cut(s) 1297
XspI CTAG 5 cut(s) 348, 432, 573, 927, 944
ZrmI AGTACT 1 cut(s) 1088
Zsp2I ATGCAT 1 cut(s) 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.