Rorug07G0068200
HSP70 Family

hsp70-Hsp90 organizing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0011330
Physical Location & Seq
Reverse (-)
5184915 .. 5191346
6432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0068200.1

Sequence Viewer

Length: 2484 bp
ATGGCCTTCGCAACCGCCAAAATTTCCAAAATGCCACTCAGAAACTCACTCCCGCTCACCTCTCACTCCCCTTCCTCTCTCAACCTCCTCTTCTCGTCCCCAAAACCGTCATTTCACATCCTCAAGCCCTTCTCTGCTCTCAGAACCACCGAGCACGGCGGCAACCCGAACGCGAGGCCGTCGCCGCACAAGTCCAAGCCCTCGTCGTCCTCCTCCACCGCGCCGTGGCTGAATAAGTGGCCGTCTCGCGGTGCTCCGTCGGAGTTGCCCCGCCGTAAGGTCAACGATAGAGTCAAGGAGTCCAACGGCGGTGGAGAGAAGCCGAGTAATGGCGGTAGGTACGTGGACAAGGACAAAGGCCAGAGCGCGATAGAGAGGATCGTGTTCCGGTTGCGGAACCTAGGGTTAGGCGACGAAGACGAGGAGAGCGGTGATGGTGTTGAATTGGACGGTCCGGATTCAATGCCGGCGGCGAGCGGAGAGGAGAAGCTCGGCGATTTGCTGCAGCGGGAGTGGGTCCGGCCGGACTATATATTGGCGGAGGAGAAGGGCGACGATGACGTGGCGCTGCCGTGGGAGAAGGAGGAGGAGGAGGAGGAATTGAGTGAGGATGAGGAGGTGAAGGGGCTGAGGAGAGCTCGGAGGTCGAAGGCGCCGAGCTTGGCTGAGCTGACGATTGAGGATGAGGAGCTGAGGCGATTGAGGAGGTTGGGAATGTTCCTGAGGGAGAGGATCAGTGTGCCGAAAGCCGGGATTACGCAGGCAGTGCTGGAGAAGATTCATGATAAGTGGAGGAAGGAGGAGCTGGTGAGGCTCAAGTTTCATGAGGTTTTGGCTCATGATATGAAAACTGCTCATGAGATTGTTGAGCGTCGAACAGGAGGTTTAGTTTTATGGAGATCAGGGAGTGTCATGGTTGTGTACCGTGGGATTAACTACAAAGGCCCTTCTAAATCCGAACCTGTTGGTAGGGAAGGAAATGCTCTTTTCATCCCAGATGTTTCATCAGCTGAAACTCCGGTAACAAGAAGTGGCAATGATGTGACTTCAACTCCAGATAAGACTGAGCAAGCTGTGAAAATTCCAGCACCTCTTCCGAAGATGACAGAGGAAGAAGCTGAATTCAACAGCTTATTGGATGAATTAGGTCCTCGTTTTGTTGAGTATTGGGGTACAGGAATACTTCCTGTTGACGCTGATTTACTTCCTAAAACAATCCCGGGTTACAAAACACCTTTCAGGCTTCTTCCTACCGGGATGCGATCACGGCTGACTAATGCAGAGATGACTAATTTACGGAAACTTGCTAAATCAATTCCATGTCATTTTGCCCTTGGGAGAAATAGAAACCATCAAGGATTAGCATCTGCTATACTTAAAGTTTGGGAGAAAAGTTCTGTGGCGAAGATTGCTGTCAAACGAGGTATCCAGAATACAAACAACAAAATAATGGCTGAGGAGCTGAAGGCATTAACAGGTGGTGTTTTACTACTCAGAAACAAATATTACATTGTCATTTACCGTGGAAAGGACTTTATCCCAACAAGTGTAGCTGCTGCTCTGGCTGAAAGACAGGAACTAACGAAACAGGTGCAAGATGTTGAAGAGATAGTGCGTGTCAAACCAATAGATGCAGCTTCTTCAGGTACAGAGGAAGGACAGGCACTTGCAGGAACTTTGGCAGAGTTCTATGAGGCCCAAGCCCGATGGGGAAGAGAAATATCTGCAGAAGAACGTGAAAAGATGATTGAAGAAGATTCAAAAGCTAAGATTGCCAGGCGTGCAAAACGAATTGAACATAAGCTAGGCGTTGCCCAAGCTAAAAAGCTTAGAGCAGAGAGACTCCTTTCCAAGATAGAATCAGCAATGCTTCCTGCAGGTCCTGATTATGATCAGGAAACCATCACTGATGAGGAGCGAGTTATGTTTCGCAGGGTTGGCTTAAGAATGAAAGCATACTTGCCTCTTGGCATACGTGGTGTCTTTGATGGTGTCATCGAAAACATGCATTTGCATTGGAAGCACAGAGAGCTAGTGAAATTAATATCCAAACAGAAGACCCTTGCTTTCGTTGAAGATTCAGCCCGGTTGTTGGAATATGAGAGCGGTGGCATACTGGTGGCTATAGAAAGAGTTCCCAAAGGATATGCTCTTATATACTATCGTGGAAAGAATTATAGGCGTCCCATTACCTTGAGGCCAAGGAACCTTTTAACTAAAGCGAAGGCATTGAAACGTTCAGTGGCCATGCAACGCCATGAGGCTCTCAGTCAGCACATAGGGGAATTGGAGAGGACCATAGAGCAAATGAGATCTGAAATTGGTATTTCTGAAGATGTAGAAGATGAGAGCACTTGGACCTCAAGAAATCCCCATCAGAGTGGTCATGACTCGGAATTTAACCAAAGTGAGGATGAAGCTTCTGACATGGAGTCAGATGGTAGTGACGATGATGAAGGCTCAGATTGGGAAGATGAAGATTAA

Protein Analysis

827

Amino Acids

92.94

Weight (kDa)

6.49

Isoelectric Point (pI)

58.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 227 - 309 4.6e-20 CRS1 / YhbY (CRM) domain
CRS1_YhbY PF01985 424 - 508 1.7e-15 CRS1 / YhbY (CRM) domain
CRS1_YhbY PF01985 635 - 722 1.4e-13 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62740 AT4G12400 AT4G12400
fragaria_vesca FvH4_5g00400 FvH4_5g00400 FvH4_5g00400
malus_domestica MD04G1081900.v1.1 MD06G1065400.v1.1
prunus_persica Prupe.5G076500_v2.0.a1
pyrus_communis pycom04g07380 pycom06g05640
rosa_chinensis RchiOBHm_Chr7g0202131
rosa_laevigata RLG00000003623
rosa_multiflora Rmu_ssc0000228.1_g000003
rosa_roxburghii Rroxscaffold_3G00254830
rosa_rugosa Rorug07G0068200 Rorug07G0068300
rosa_samantha Rh7AG195700 Rh7CG206800
rosa_wichuraiana Rw7G017140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1868
AccB1I GGYRCC 1 cut(s) 652
AccBSI CCGCTC 4 cut(s) 55, 429, 477, 2106
AccII CGCG 4 cut(s) 173, 221, 249, 368
AccIII TCCGGA 1 cut(s) 454
AclI AACGTT 1 cut(s) 2236
AclWI GGATC 2 cut(s) 386, 740
AcoI YGGCCR 3 cut(s) 239, 521, 2244
AcsI RAATTY 4 cut(s) 21, 1080, 1121, 2396
AcuI CTGAAG 3 cut(s) 1484, 1626, 2352
AcyI GRCGYC 2 cut(s) 653, 2182
AfaI GTAC 4 cut(s) 341, 923, 1174, 1648
AfiI CCNNNNNNNGG 8 cut(s) 225, 248, 277, 329, 749, 1528, 2092, 2410
AflII CTTAAG 1 cut(s) 1942
AhdI GACNNNNNGTC 1 cut(s) 2431
AjiI CACGTC 1 cut(s) 562
AjnI CCWGG 1 cut(s) 1775
Alw21I GWGCWC 4 cut(s) 156, 256, 640, 2354
Alw26I GTCTC 2 cut(s) 249, 1834
AlwI GGATC 2 cut(s) 386, 740
AlwNI CAGNNNCTG 1 cut(s) 1883
Ama87I CYCGRG 1 cut(s) 1219
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 9 cut(s) 3, 176, 239, 358, 521, 943, 1695, 2198, 2244
ApeKI GCWGC 6 cut(s) 502, 505, 568, 1553, 1556, 1634
ApoI RAATTY 4 cut(s) 21, 1080, 1121, 2396
AseI ATTAAT 1 cut(s) 2042
Asp700I GAANNNNTTC 1 cut(s) 2133
AspA2I CCTAGG 1 cut(s) 400
AspLEI GCGC 4 cut(s) 223, 368, 568, 655
AspS9I GGNCC 8 cut(s) 452, 517, 944, 1148, 1696, 1880, 2295, 2358
AsuC2I CCSGG 5 cut(s) 751, 1220, 1221, 1255, 2086
AsuHPI GGTGA 4 cut(s) 49, 443, 631, 820
AvaI CYCGRG 1 cut(s) 1219
AvaII GGWCC 6 cut(s) 452, 517, 1148, 1880, 2295, 2358
AvrII CCTAGG 1 cut(s) 400
AxyI CCTNAGG 1 cut(s) 722
BalI TGGCCA 1 cut(s) 2246
BanI GGYRCC 1 cut(s) 652
BanII GRGCYC 1 cut(s) 640
BbsI GAAGAC 2 cut(s) 423, 2063
Bbv12I GWGCWC 4 cut(s) 156, 256, 640, 2354
BbvCI CCTCAGC 3 cut(s) 629, 692, 1455
BbvI GCAGC 6 cut(s) 489, 517, 555, 1540, 1543, 1646
BccI CCATC 7 cut(s) 428, 1359, 1701, 1910, 1982, 2382, 2432
BceAI ACGGC 8 cut(s) 163, 172, 208, 226, 258, 322, 556, 1283
BcgI CGANNNNNNTGC 2 cut(s) 1573, 1607
BciT130I CCWGG 1 cut(s) 1777
BciVI GTATCC 1 cut(s) 1436
BclI TGATCA 1 cut(s) 1891
BcnI CCSGG 5 cut(s) 751, 1220, 1221, 1255, 2086
BcoDI GTCTC 2 cut(s) 249, 1834
BfaI CTAG 3 cut(s) 401, 1805, 2033
BfmI CTRYAG 4 cut(s) 503, 1725, 1875, 2124
BfoI RGCGCY 2 cut(s) 569, 656
BfrI CTTAAG 1 cut(s) 1942
BfuAI ACCTGC 1 cut(s) 1868
BfuI GTATCC 1 cut(s) 1436
BglII AGATCT 1 cut(s) 2312
BisI GCNGC 9 cut(s) 160, 185, 471, 503, 506, 569, 1554, 1557, 1635
BlnI CCTAGG 1 cut(s) 400
BlpI GCTNAGC 1 cut(s) 666
BlsI GCNGC 9 cut(s) 161, 186, 472, 504, 507, 570, 1555, 1558, 1636
Bme1390I CCNGG 6 cut(s) 751, 1220, 1221, 1255, 1777, 2086
Bme18I GGWCC 6 cut(s) 452, 517, 1148, 1880, 2295, 2358
BmeRI GACNNNNNGTC 1 cut(s) 2431
BmeT110I CYCGRG 1 cut(s) 1219
BmgBI CACGTC 1 cut(s) 562
BmgT120I GGNCC 8 cut(s) 452, 517, 944, 1148, 1696, 1880, 2295, 2358
BmiI GGNNCC 4 cut(s) 398, 518, 654, 2207
BmrFI CCNGG 6 cut(s) 751, 1220, 1221, 1255, 1777, 2086
BmsI GCATC 3 cut(s) 1248, 1373, 1621
BpiI GAAGAC 2 cut(s) 423, 2063
BplI GAGNNNNNCTC 2 cut(s) 622, 654
BpmI CTGGAG 2 cut(s) 791, 1038
Bpu10I CCTNAGC 3 cut(s) 629, 692, 1455
Bpu1102I GCTNAGC 1 cut(s) 666
BpuEI CTTGAG 4 cut(s) 107, 800, 2215, 2347
BpuMI CCSGG 5 cut(s) 751, 1220, 1221, 1255, 2086
BsaAI YACGTR 2 cut(s) 343, 1976
BsaBI GATNNNNATC 2 cut(s) 1363, 1890
BsaHI GRCGYC 2 cut(s) 653, 2182
BsaJI CCNNGG 8 cut(s) 224, 400, 572, 925, 1219, 1333, 1522, 2201
BsaWI WCCGGW 3 cut(s) 387, 454, 1018
BsaXI ACNNNNNCTCC 4 cut(s) 873, 903, 1036, 1066
Bsc4I CCNNNNNNNGG 8 cut(s) 225, 248, 277, 329, 749, 1528, 2092, 2410
Bse118I RCCGGY 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 2121
Bse21I CCTNAGG 1 cut(s) 722
Bse3DI GCAATG 2 cut(s) 1042, 1872
Bse8I GATNNNNATC 2 cut(s) 1363, 1890
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 1 cut(s) 1777
BseDI CCNNGG 8 cut(s) 224, 400, 572, 925, 1219, 1333, 1522, 2201
BseGI GGATG 7 cut(s) 117, 616, 688, 990, 1144, 1263, 2419
BseJI GATNNNNATC 2 cut(s) 1363, 1890
BseLI CCNNNNNNNGG 8 cut(s) 225, 248, 277, 329, 749, 1528, 2092, 2410
BseMI GCAATG 2 cut(s) 1042, 1872
BseNI ACTGG 1 cut(s) 2121
BseX3I CGGCCG 1 cut(s) 521
BseXI GCAGC 6 cut(s) 489, 517, 555, 1540, 1543, 1646
Bsh1236I CGCG 4 cut(s) 173, 221, 249, 368
Bsh1285I CGRYCG 1 cut(s) 524
BshFI GGCC 9 cut(s) 5, 178, 241, 360, 523, 945, 1697, 2200, 2246
BshNI GGYRCC 1 cut(s) 652
BsiEI CGRYCG 1 cut(s) 524
BsiHKAI GWGCWC 4 cut(s) 156, 256, 640, 2354
BsiHKCI CYCGRG 1 cut(s) 1219
BslFI GGGAC 2 cut(s) 82, 2169
BslI CCNNNNNNNGG 8 cut(s) 225, 248, 277, 329, 749, 1528, 2092, 2410
BsmAI GTCTC 2 cut(s) 249, 1834
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 2 cut(s) 82, 2169
BsnI GGCC 9 cut(s) 5, 178, 241, 360, 523, 945, 1697, 2200, 2246
BsoBI CYCGRG 1 cut(s) 1219
Bsp1286I GDGCHC 4 cut(s) 156, 256, 640, 2354
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 6 cut(s) 378, 732, 899, 1262, 1891, 2312
Bsp1720I GCTNAGC 1 cut(s) 666
BspANI GGCC 9 cut(s) 5, 178, 241, 360, 523, 945, 1697, 2200, 2246
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 4 cut(s) 173, 221, 249, 368
BspHI TCATGA 5 cut(s) 781, 823, 838, 856, 2386
BspLI GGNNCC 4 cut(s) 398, 518, 654, 2207
BspMAI CTGCAG 3 cut(s) 507, 1729, 1879
BspMI ACCTGC 1 cut(s) 1868
BspPI GGATC 2 cut(s) 386, 740
BspT107I GGYRCC 1 cut(s) 652
BspTI CTTAAG 1 cut(s) 1942
BsrBI CCGCTC 4 cut(s) 55, 429, 477, 2106
BsrDI GCAATG 2 cut(s) 1042, 1872
BsrFI RCCGGY 1 cut(s) 466
BsrI ACTGG 1 cut(s) 2121
BssAI RCCGGY 1 cut(s) 466
BssECI CCNNGG 8 cut(s) 224, 400, 572, 925, 1219, 1333, 1522, 2201
BssMI GATC 6 cut(s) 378, 732, 899, 1262, 1891, 2312
BssNI GRCGYC 2 cut(s) 653, 2182
BssT1I CCWWGG 3 cut(s) 400, 1333, 2201
Bst2UI CCWGG 1 cut(s) 1777
Bst4CI ACNGT 4 cut(s) 108, 452, 926, 1523
Bst6I CTCTTC 4 cut(s) 95, 1098, 1599, 1708
BstACI GRCGYC 2 cut(s) 653, 2182
BstAFI CTTAAG 1 cut(s) 1942
BstAPI GCANNNNNTGC 1 cut(s) 766
BstBAI YACGTR 2 cut(s) 343, 1976
BstC8I GCNNGC 5 cut(s) 468, 475, 762, 1071, 1782
BstDSI CCRYGG 4 cut(s) 224, 572, 925, 1522
BstF5I GGATG 7 cut(s) 117, 616, 688, 990, 1144, 1263, 2419
BstFNI CGCG 4 cut(s) 173, 221, 249, 368
BstH2I RGCGCY 2 cut(s) 569, 656
BstHHI GCGC 4 cut(s) 223, 368, 568, 655
BstKTI GATC 6 cut(s) 381, 735, 902, 1265, 1894, 2315
BstMAI GTCTC 2 cut(s) 249, 1834
BstMBI GATC 6 cut(s) 378, 732, 899, 1262, 1891, 2312
BstMCI CGRYCG 1 cut(s) 524
BstNI CCWGG 1 cut(s) 1777
BstNSI RCATGY 1 cut(s) 2008
BstSCI CCNGG 6 cut(s) 749, 1218, 1219, 1253, 1775, 2084
BstSFI CTRYAG 4 cut(s) 503, 1725, 1875, 2124
BstUI CGCG 4 cut(s) 173, 221, 249, 368
BstV1I GCAGC 6 cut(s) 489, 517, 555, 1540, 1543, 1646
BstV2I GAAGAC 2 cut(s) 423, 2063
BstX2I RGATCY 1 cut(s) 2312
BstXI CCANNNNNNTGG 1 cut(s) 2381
BstYI RGATCY 1 cut(s) 2312
BstZI CGGCCG 1 cut(s) 521
Bsu36I CCTNAGG 1 cut(s) 722
BsuI GTATCC 1 cut(s) 1436
BsuRI GGCC 9 cut(s) 5, 178, 241, 360, 523, 945, 1697, 2200, 2246
BtgI CCRYGG 4 cut(s) 224, 572, 925, 1522
BtrI CACGTC 1 cut(s) 562
BtsCI GGATG 7 cut(s) 117, 616, 688, 990, 1144, 1263, 2419
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 4 cut(s) 742, 771, 1905, 2247
BveI ACCTGC 1 cut(s) 1868
Cac8I GCNNGC 5 cut(s) 468, 475, 762, 1071, 1782
CaiI CAGNNNCTG 1 cut(s) 1883
CciI TCATGA 5 cut(s) 781, 823, 838, 856, 2386
CfoI GCGC 4 cut(s) 223, 368, 568, 655
Cfr10I RCCGGY 1 cut(s) 466
Cfr13I GGNCC 8 cut(s) 452, 517, 944, 1148, 1696, 1880, 2295, 2358
Cfr9I CCCGGG 1 cut(s) 1219
CpoI CGGWCCG 1 cut(s) 452
CseI GACGC 3 cut(s) 860, 1202, 2171
Csp6I GTAC 4 cut(s) 340, 922, 1173, 1647
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CspI CGGWCCG 1 cut(s) 452
CviQI GTAC 4 cut(s) 340, 922, 1173, 1647
DinI GGCGCC 1 cut(s) 654
DpnI GATC 6 cut(s) 380, 734, 901, 1264, 1893, 2314
DpnII GATC 6 cut(s) 378, 732, 899, 1262, 1891, 2312
DriI GACNNNNNGTC 1 cut(s) 2431
EaeI YGGCCR 3 cut(s) 239, 521, 2244
EagI CGGCCG 1 cut(s) 521
Eam1104I CTCTTC 4 cut(s) 95, 1098, 1599, 1708
Eam1105I GACNNNNNGTC 1 cut(s) 2431
EarI CTCTTC 4 cut(s) 95, 1098, 1599, 1708
EciI GGCGGA 1 cut(s) 554
Ecl136II GAGCTC 1 cut(s) 638
EclXI CGGCCG 1 cut(s) 521
Eco130I CCWWGG 3 cut(s) 400, 1333, 2201
Eco24I GRGCYC 1 cut(s) 640
Eco47I GGWCC 6 cut(s) 452, 517, 1148, 1880, 2295, 2358
Eco52I CGGCCG 1 cut(s) 521
Eco53kI GAGCTC 1 cut(s) 638
Eco57I CTGAAG 3 cut(s) 1484, 1626, 2352
Eco81I CCTNAGG 1 cut(s) 722
Eco88I CYCGRG 1 cut(s) 1219
EcoICRI GAGCTC 1 cut(s) 638
EcoO109I RGGNCCY 3 cut(s) 944, 1148, 1880
EcoRI GAATTC 1 cut(s) 1121
EcoRII CCWGG 1 cut(s) 1775
EcoT14I CCWWGG 3 cut(s) 400, 1333, 2201
EcoT22I ATGCAT 1 cut(s) 2010
EcoT38I GRGCYC 1 cut(s) 640
EgeI GGCGCC 1 cut(s) 654
EheI GGCGCC 1 cut(s) 654
ErhI CCWWGG 3 cut(s) 400, 1333, 2201
Esp3I CGTCTC 1 cut(s) 249
FaqI GGGAC 2 cut(s) 82, 2169
FauI CCCGC 3 cut(s) 60, 278, 501
FbaI TGATCA 1 cut(s) 1891
Fnu4HI GCNGC 9 cut(s) 160, 185, 471, 503, 506, 569, 1554, 1557, 1635
FokI GGATG 7 cut(s) 104, 623, 695, 977, 1151, 1270, 2426
FriOI GRGCYC 1 cut(s) 640
Fsp4HI GCNGC 9 cut(s) 160, 185, 471, 503, 506, 569, 1554, 1557, 1635
FspBI CTAG 3 cut(s) 401, 1805, 2033
GlaI GCGC 4 cut(s) 222, 367, 567, 654
GluI GCNGC 9 cut(s) 160, 185, 471, 503, 506, 569, 1554, 1557, 1635
GsuI CTGGAG 2 cut(s) 791, 1038
HaeII RGCGCY 2 cut(s) 569, 656
HaeIII GGCC 9 cut(s) 5, 178, 241, 360, 523, 945, 1697, 2200, 2246
HgaI GACGC 3 cut(s) 860, 1202, 2171
HhaI GCGC 4 cut(s) 223, 368, 568, 655
Hin1I GRCGYC 2 cut(s) 653, 2182
Hin6I GCGC 4 cut(s) 221, 366, 566, 653
HinP1I GCGC 4 cut(s) 221, 366, 566, 653
HincII GTYRAC 2 cut(s) 283, 1192
HindII GTYRAC 2 cut(s) 283, 1192
HindIII AAGCTT 2 cut(s) 1826, 2418
HphI GGTGA 4 cut(s) 49, 443, 631, 820
Hpy166II GTNNAC 4 cut(s) 283, 346, 922, 1192
Hpy8I GTNNAC 4 cut(s) 283, 346, 922, 1192
Hpy99I CGWCG 6 cut(s) 184, 208, 262, 416, 557, 876
HpyCH4III ACNGT 4 cut(s) 108, 452, 926, 1523
HpyCH4IV ACGT 5 cut(s) 342, 561, 1735, 1975, 2236
HpySE526I ACGT 5 cut(s) 342, 561, 1735, 1975, 2236
Hsp92I GRCGYC 2 cut(s) 653, 2182
HspAI GCGC 4 cut(s) 221, 366, 566, 653
KasI GGCGCC 1 cut(s) 652
Kpn2I TCCGGA 1 cut(s) 454
KroI GCCGGC 1 cut(s) 466
KroNI GCCGGC 1 cut(s) 468
Ksp22I TGATCA 1 cut(s) 1891
Kzo9I GATC 6 cut(s) 378, 732, 899, 1262, 1891, 2312
LmnI GCTCC 5 cut(s) 259, 688, 802, 1459, 1915
Lsp1109I GCAGC 6 cut(s) 489, 517, 555, 1540, 1543, 1646
LweI GCATC 3 cut(s) 1248, 1373, 1621
MaeI CTAG 3 cut(s) 401, 1805, 2033
MaeII ACGT 5 cut(s) 342, 561, 1735, 1975, 2236
MaeIII GTNAC 4 cut(s) 1021, 1042, 1223, 2444
MalI GATC 6 cut(s) 380, 734, 901, 1264, 1893, 2314
MbiI CCGCTC 4 cut(s) 55, 429, 477, 2106
MboI GATC 6 cut(s) 378, 732, 899, 1262, 1891, 2312
MflI RGATCY 1 cut(s) 2312
MhlI GDGCHC 4 cut(s) 156, 256, 640, 2354
MlsI TGGCCA 1 cut(s) 2246
MluNI TGGCCA 1 cut(s) 2246
Mly113I GGCGCC 1 cut(s) 653
MlyI GAGTC 5 cut(s) 300, 308, 1836, 2384, 2441
MmeI TCCRAC 3 cut(s) 240, 327, 2073
Mox20I TGGCCA 1 cut(s) 2246
Mph1103I ATGCAT 1 cut(s) 2010
MroI TCCGGA 1 cut(s) 454
MroNI GCCGGC 1 cut(s) 466
MroXI GAANNNNTTC 1 cut(s) 2133
MscI TGGCCA 1 cut(s) 2246
MseI TTAA 8 cut(s) 933, 1377, 1472, 1943, 2042, 2213, 2400, 2482
MslI CAYNNNNRTG 3 cut(s) 917, 2117, 2379
Msp20I TGGCCA 1 cut(s) 2246
MspA1I CMGCKG 2 cut(s) 508, 1010
MspCI CTTAAG 1 cut(s) 1942
MspR9I CCNGG 6 cut(s) 751, 1220, 1221, 1255, 1777, 2086
MvaI CCWGG 1 cut(s) 1777
MvnI CGCG 4 cut(s) 173, 221, 249, 368
NaeI GCCGGC 1 cut(s) 468
NarI GGCGCC 1 cut(s) 653
NciI CCSGG 5 cut(s) 751, 1220, 1221, 1255, 2086
NdeII GATC 6 cut(s) 378, 732, 899, 1262, 1891, 2312
NgoMIV GCCGGC 1 cut(s) 466
NlaIV GGNNCC 4 cut(s) 398, 518, 654, 2207
NmeAIII GCCGAG 3 cut(s) 348, 471, 681
NmuCI GTSAC 2 cut(s) 1042, 2444
NsiI ATGCAT 1 cut(s) 2010
NspI RCATGY 1 cut(s) 2008
PagI TCATGA 5 cut(s) 781, 823, 838, 856, 2386
PcsI WCGNNNNNNNCGW 1 cut(s) 653
PdiI GCCGGC 1 cut(s) 468
PdmI GAANNNNTTC 1 cut(s) 2133
PfeI GAWTC 5 cut(s) 458, 778, 1757, 1859, 2078
PkrI GCNGC 9 cut(s) 161, 186, 472, 504, 507, 570, 1555, 1558, 1636
PleI GAGTC 5 cut(s) 299, 307, 1836, 2384, 2440
PluTI GGCGCC 1 cut(s) 656
PpsI GAGTC 5 cut(s) 299, 307, 1836, 2384, 2440
Ppu21I YACGTR 2 cut(s) 343, 1976
PpuMI RGGWCCY 2 cut(s) 1148, 1880
PshBI ATTAAT 1 cut(s) 2042
Psp124BI GAGCTC 1 cut(s) 640
Psp1406I AACGTT 1 cut(s) 2236
Psp5II RGGWCCY 2 cut(s) 1148, 1880
Psp6I CCWGG 1 cut(s) 1775
PspGI CCWGG 1 cut(s) 1775
PspN4I GGNNCC 4 cut(s) 398, 518, 654, 2207
PspPI GGNCC 8 cut(s) 452, 517, 944, 1148, 1696, 1880, 2295, 2358
PspPPI RGGWCCY 2 cut(s) 1148, 1880
PstI CTGCAG 3 cut(s) 507, 1729, 1879
PstNI CAGNNNCTG 1 cut(s) 1883
PsuI RGATCY 1 cut(s) 2312
PvuII CAGCTG 1 cut(s) 1010
RsaI GTAC 4 cut(s) 341, 923, 1174, 1648
RsaNI GTAC 4 cut(s) 340, 922, 1173, 1647
RseI CAYNNNNRTG 3 cut(s) 917, 2117, 2379
Rsr2I CGGWCCG 1 cut(s) 452
RsrII CGGWCCG 1 cut(s) 452
SacI GAGCTC 1 cut(s) 640
SaqAI TTAA 8 cut(s) 933, 1377, 1472, 1943, 2042, 2213, 2400, 2482
SatI GCNGC 9 cut(s) 160, 185, 471, 503, 506, 569, 1554, 1557, 1635
Sau3AI GATC 6 cut(s) 378, 732, 899, 1262, 1891, 2312
Sau96I GGNCC 8 cut(s) 452, 517, 944, 1148, 1696, 1880, 2295, 2358
SbfI CCTGCAGG 1 cut(s) 1879
SchI GAGTC 5 cut(s) 300, 308, 1836, 2384, 2441
ScrFI CCNGG 6 cut(s) 751, 1220, 1221, 1255, 1777, 2086
SdaI CCTGCAGG 1 cut(s) 1879
SduI GDGCHC 4 cut(s) 156, 256, 640, 2354
SfaNI GCATC 3 cut(s) 1248, 1373, 1621
SfcI CTRYAG 4 cut(s) 503, 1725, 1875, 2124
SfoI GGCGCC 1 cut(s) 654
SinI GGWCC 6 cut(s) 452, 517, 1148, 1880, 2295, 2358
SmaI CCCGGG 1 cut(s) 1221
SmiMI CAYNNNNRTG 3 cut(s) 917, 2117, 2379
SmlI CTYRAG 5 cut(s) 122, 815, 1942, 2194, 2362
SmoI CTYRAG 5 cut(s) 122, 815, 1942, 2194, 2362
Sse8387I CCTGCAGG 1 cut(s) 1879
SspDI GGCGCC 1 cut(s) 652
SspI AATATT 1 cut(s) 1505
SspMI CTAG 3 cut(s) 401, 1805, 2033
SstI GAGCTC 1 cut(s) 640
StyD4I CCNGG 6 cut(s) 749, 1218, 1219, 1253, 1775, 2084
StyI CCWWGG 3 cut(s) 400, 1333, 2201
TaaI ACNGT 4 cut(s) 108, 452, 926, 1523
TaiI ACGT 5 cut(s) 345, 564, 1738, 1978, 2239
TaqI TCGA 3 cut(s) 647, 874, 1998
TauI GCSGC 3 cut(s) 162, 187, 473
TfiI GAWTC 5 cut(s) 458, 778, 1757, 1859, 2078
Tru1I TTAA 8 cut(s) 933, 1377, 1472, 1943, 2042, 2213, 2400, 2482
Tru9I TTAA 8 cut(s) 933, 1377, 1472, 1943, 2042, 2213, 2400, 2482
TscAI CASTG 4 cut(s) 742, 771, 1912, 2247
TseFI GTSAC 2 cut(s) 1042, 2444
TseI GCWGC 6 cut(s) 502, 505, 568, 1553, 1556, 1634
Tsp45I GTSAC 2 cut(s) 1042, 2444
TspDTI ATGAA 9 cut(s) 770, 812, 860, 979, 993, 1155, 1964, 2430, 2469
TspGWI ACGGA 2 cut(s) 246, 1312
TspMI CCCGGG 1 cut(s) 1219
TspRI CASTG 4 cut(s) 742, 771, 1912, 2247
Vha464I CTTAAG 1 cut(s) 1942
VpaK11BI GGWCC 6 cut(s) 452, 517, 1148, 1880, 2295, 2358
VspI ATTAAT 1 cut(s) 2042
XapI RAATTY 4 cut(s) 21, 1080, 1121, 2396
XceI RCATGY 1 cut(s) 2008
XmaI CCCGGG 1 cut(s) 1219
XmaJI CCTAGG 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 2133
XspI CTAG 3 cut(s) 401, 1805, 2033
Zsp2I ATGCAT 1 cut(s) 2010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.