Rroxscaffold_3G00254830
HSP70 Family

hsp70-Hsp90 organizing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011330
Physical Location & Seq
Forward (+)
49146337 .. 49149619
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00254830.1

Sequence Viewer

Length: 1746 bp
ATGGCCGACGAAGCCAAAGCCAAGGGCAACGCCGCCTACTCGGCCGGCGACTACACCGCCGCCATTACCCACTTCACCGAAGCCATCAACCTCGCTCCCACCAACCACGTCCTCTACTCCAACCGATCCGCCTCCTACGCCAGCCTCCACAAATACTCCGACGCGCTCTCCGACGCCAAGAAGACCGTCGAGCTCAAGCCCGACTGGGTCAAAGGCTACAGCCGCCTCGGCTCCGCCCACCACGGCCTCGGCCACTTCGACGACGCCGTTTCCGCTTACCAGAAGGGCCTCGAGATTGATCCCAACAACGCCGCCCTTAAAGAGGGCTTGGCCGAGAGTCAGGCCGCCGCCGCCCGCGCCGCCCGAGCACGAGCCCAGCCGCCGCCGAACCTCTTCGGCGACGCGTTCTCGGGTCCTCAGATGTGGGCCAAGCTCACGGCCGACCCGAGCACCCGGGCCTTTATGCAGCAGCCGGATTTCGTGAACATGATGCAGGAGATTCAGAAAAACCCTAGCAATCTCAATCTTTATTTGAAGGATCAGAGGGTTATGCAAGCCCTAGGGGTTTTGCTCAATGTGAAGCTCCAGGGAGGCGCGTCGGAGGACGACGATATGCCGGATTTTCCGTCGGAGAGGCCGCGGTCTGCGGAGGCCGCTGCTGAGCCGCAGAAGAAGGAGGAGAAGAAGCCGGAGCCGGAGCCGGAGCCGATGGAAGTGCCGGAGGAGGAGAAGGAGTCCAGGAAGAGGAAAGCTGAGGCTTTGAAGGAGAAGGAGGCTGGCAATGCGGCTTATAAGAAGAAGGATTTCGACACTGCTATTCAGCACTATACTAAGGCAATGGAGATAGATGATGAAGATATTTCGTACTTGTTGAATCGTGCTGCGACTTATTTGGAAATGGGTCAGTATGATGAATGCATTAAAGACTGTGATAAGGCTGTGGAAAGGGGCAGAGAGGTAAGAGCGGACTTTAAGATGATAGCAAAAGCTTTGACTAGGAAGGGAACTGCCATTGTGAAAACAGCAAAAACCTCGAAGGACTATGAGCCTGCTATTGAGATATTCCAGAAAGCTCTCACAGAGCATCGCAACCCTGACACCCTAAAGAAATTGAATGATGCTGAGAAAGCTAAGAAGGACCTAGAGCAACAAGAGTATTTTGATCCAAAGCTAGCAGATGAGGAGCGAGAGAAAGGCAACGAATTCTTCAAGCAGCAGAAGTACCCAGAGGCTATCAGGCATTACACTGAATCTTTGAGAAGGAATCCAAAGGATCCAAAGGCATATAGCAATAGAGCTGCTTGCTATACAAAGTTGGGGGCAATGCCCGAGGGATTGAAGGATGCTGAGAAGTGCATTGAGCTTGACCCAACCTTTTCAAAGGGTTATACCAGAAAAGGTGCAGTCCAGTTTTTCATGAGGGAGTATGACAAAGCTTTGGAAACATATCAAGAAGGTCTGAAGCATGATCCTGGGAACCAGGACTTGCTTGATGGTGTGAGGAGATGTGTAGAGCAGATCAACAAAGCTAGCCGTGGGGATCTGAGTGCCGATGAATTGAAGGAGAGACAGACTAAGGGAATGCAGGACCCAGAAATTCAGAATATCCTTTCAGATCCTGTTATGAGACAGGTGTTGGTTGATTTCCAGGAAAATCCCAAAGCTGCTCAGGAACACTCAAAGAACCCGATGGTGATGGCCAAAATCCAGAAGCTGGTCAGTGCTGGAATTGTCCAGATTAAGTGA

Protein Analysis

581

Amino Acids

65.3

Weight (kDa)

6.02

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 4 - 70 9.5e-07 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 71 - 103 1.9e-07 Tetratricopeptide repeat
TPR_2 PF07719 71 - 103 2e-06 Tetratricopeptide repeat
STI1-HOP_DP PF17830 139 - 191 2.8e-22 STI1/HOP, DP domain
TPR_BSK1_C PF25575 250 - 319 4.8e-08 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_11 PF13414 260 - 301 1.9e-06 TPR repeat
TPR_1 PF00515 290 - 315 4e-06 Tetratricopeptide repeat
TPR_12 PF13424 290 - 359 5e-07 Tetratricopeptide repeat
TPR_BSK1_C PF25575 372 - 459 9.9e-06 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 398 - 425 9.5e-06 Tetratricopeptide repeat
TPR_16 PF13432 398 - 449 2.4e-06 Tetratricopeptide repeat
TPR_11 PF13414 399 - 440 1.2e-08 TPR repeat
TPR_1 PF00515 426 - 459 1.2e-06 Tetratricopeptide repeat
TPR_1 PF00515 461 - 493 4.1e-06 Tetratricopeptide repeat
STI1-HOP_DP PF17830 522 - 573 3.7e-15 STI1/HOP, DP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62740 AT4G12400 AT4G12400
fragaria_vesca FvH4_5g00400 FvH4_5g00400 FvH4_5g00400
malus_domestica MD04G1081900.v1.1 MD06G1065400.v1.1
prunus_persica Prupe.5G076500_v2.0.a1
pyrus_communis pycom04g07380 pycom06g05640
rosa_chinensis RchiOBHm_Chr7g0202131
rosa_laevigata RLG00000003623
rosa_multiflora Rmu_ssc0000228.1_g000003
rosa_roxburghii Rroxscaffold_3G00254830
rosa_rugosa Rorug07G0068200 Rorug07G0068300
rosa_samantha Rh7AG195700 Rh7CG206800
rosa_wichuraiana Rw7G017140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
AccB7I CCANNNNNTGG 1 cut(s) 1714
AccBSI CCGCTC 1 cut(s) 965
AccII CGCG 5 cut(s) 164, 357, 404, 596, 640
AclWI GGATC 9 cut(s) 120, 293, 546, 1157, 1268, 1281, 1463, 1548, 1610
AcoI YGGCCR 6 cut(s) 3, 42, 250, 330, 438, 1698
AcsI RAATTY 2 cut(s) 1202, 1596
AcuI CTGAAG 1 cut(s) 1481
AcyI GRCGYC 2 cut(s) 174, 264
AfaI GTAC 2 cut(s) 866, 1223
AfiI CCNNNNNNNGG 3 cut(s) 322, 744, 1714
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 8 cut(s) 535, 763, 874, 1114, 1210, 1339, 1380, 1561
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 5 cut(s) 585, 737, 1471, 1479, 1647
Alw21I GWGCWC 3 cut(s) 195, 370, 452
Alw26I GTCTC 2 cut(s) 1561, 1621
AlwI GGATC 9 cut(s) 120, 293, 546, 1157, 1268, 1281, 1463, 1548, 1610
AlwNI CAGNNNCTG 2 cut(s) 1619, 1714
Ama87I CYCGRG 6 cut(s) 290, 363, 409, 445, 453, 1328
ApeKI GCWGC 7 cut(s) 466, 469, 656, 881, 1213, 1298, 1664
ApoI RAATTY 2 cut(s) 1202, 1596
Asp700I GAANNNNTTC 2 cut(s) 392, 803
AspA2I CCTAGG 1 cut(s) 559
AspLEI GCGC 3 cut(s) 166, 359, 596
AspS9I GGNCC 6 cut(s) 286, 413, 426, 456, 1138, 1588
AsuC2I CCSGG 2 cut(s) 454, 455
AsuHPI GGTGA 2 cut(s) 67, 1705
AsuNHI GCTAGC 2 cut(s) 1171, 1529
AvaI CYCGRG 6 cut(s) 290, 363, 409, 445, 453, 1328
AvaII GGWCC 3 cut(s) 413, 1138, 1588
AvrII CCTAGG 1 cut(s) 559
BalI TGGCCA 1 cut(s) 1700
BamHI GGATCC 1 cut(s) 1273
BanII GRGCYC 2 cut(s) 195, 376
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 3 cut(s) 195, 370, 452
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 7 cut(s) 478, 481, 643, 868, 1225, 1285, 1651
BccI CCATC 5 cut(s) 92, 703, 1487, 1684, 1690
BceAI ACGGC 4 cut(s) 251, 259, 453, 1518
BcgI CGANNNNNNTGC 4 cut(s) 697, 731, 1014, 1048
BciT130I CCWGG 5 cut(s) 587, 739, 1473, 1481, 1649
BcnI CCSGG 2 cut(s) 454, 455
BcoDI GTCTC 2 cut(s) 1561, 1621
BfaI CTAG 6 cut(s) 513, 560, 996, 1142, 1172, 1530
BfmI CTRYAG 1 cut(s) 217
BglI GCCNNNNNGGC 2 cut(s) 41, 228
BlnI CCTAGG 1 cut(s) 559
BlpI GCTNAGC 1 cut(s) 660
Bme1390I CCNGG 7 cut(s) 454, 455, 587, 739, 1473, 1481, 1649
Bme18I GGWCC 3 cut(s) 413, 1138, 1588
BmeT110I CYCGRG 6 cut(s) 290, 363, 409, 445, 453, 1328
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 6 cut(s) 286, 413, 426, 456, 1138, 1588
BmiI GGNNCC 8 cut(s) 232, 414, 693, 699, 705, 1275, 1478, 1590
BmrFI CCNGG 7 cut(s) 454, 455, 587, 739, 1473, 1481, 1649
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 4 cut(s) 480, 1093, 1108, 1333
BmtI GCTAGC 2 cut(s) 1175, 1533
BmuI ACTGGG 1 cut(s) 214
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 569
Bpu10I CCTNAGC 2 cut(s) 753, 1668
Bpu1102I GCTNAGC 1 cut(s) 660
BpuEI CTTGAG 1 cut(s) 179
BpuMI CCSGG 2 cut(s) 454, 455
BsaHI GRCGYC 2 cut(s) 174, 264
BsaXI ACNNNNNCTCC 6 cut(s) 140, 152, 170, 182, 719, 749
Bsc4I CCNNNNNNNGG 3 cut(s) 322, 744, 1714
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 2 cut(s) 209, 1408
Bse3DI GCAATG 3 cut(s) 787, 843, 1329
BseBI CCWGG 5 cut(s) 587, 739, 1473, 1481, 1649
BseGI GGATG 1 cut(s) 1348
BseLI CCNNNNNNNGG 3 cut(s) 322, 744, 1714
BseMI GCAATG 3 cut(s) 787, 843, 1329
BseMII CTCAG 7 cut(s) 431, 651, 744, 1113, 1338, 1535, 1682
BseNI ACTGG 2 cut(s) 209, 1408
BseRI GAGGAG 5 cut(s) 692, 737, 740, 1196, 1516
BseX3I CGGCCG 2 cut(s) 42, 438
BseXI GCAGC 7 cut(s) 478, 481, 643, 868, 1225, 1285, 1651
BseYI CCCAGC 1 cut(s) 375
BsgI GTGCAG 1 cut(s) 1422
Bsh1236I CGCG 5 cut(s) 164, 357, 404, 596, 640
Bsh1285I CGRYCG 2 cut(s) 45, 441
BsiEI CGRYCG 2 cut(s) 45, 441
BsiHKAI GWGCWC 3 cut(s) 195, 370, 452
BsiHKCI CYCGRG 6 cut(s) 290, 363, 409, 445, 453, 1328
BsiSI CCGG 8 cut(s) 45, 454, 473, 617, 689, 695, 701, 719
BslI CCNNNNNNNGG 3 cut(s) 322, 744, 1714
BsmAI GTCTC 2 cut(s) 1561, 1621
BsmI GAATGC 2 cut(s) 920, 1587
BsoBI CYCGRG 6 cut(s) 290, 363, 409, 445, 453, 1328
Bsp1286I GDGCHC 4 cut(s) 195, 370, 376, 452
Bsp143I GATC 9 cut(s) 125, 298, 538, 1162, 1273, 1468, 1518, 1540, 1615
Bsp1720I GCTNAGC 1 cut(s) 660
BspCNI CTCAG 7 cut(s) 430, 652, 745, 1114, 1339, 1536, 1681
BspFNI CGCG 5 cut(s) 164, 357, 404, 596, 640
BspHI TCATGA 1 cut(s) 1416
BspLI GGNNCC 8 cut(s) 232, 414, 693, 699, 705, 1275, 1478, 1590
BspOI GCTAGC 2 cut(s) 1175, 1533
BspPI GGATC 9 cut(s) 120, 293, 546, 1157, 1268, 1281, 1463, 1548, 1610
BsrBI CCGCTC 1 cut(s) 965
BsrDI GCAATG 3 cut(s) 787, 843, 1329
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 2 cut(s) 209, 1408
BssAI RCCGGY 1 cut(s) 44
BssMI GATC 9 cut(s) 125, 298, 538, 1162, 1273, 1468, 1518, 1540, 1615
BssNI GRCGYC 2 cut(s) 174, 264
BssSI CACGAG 1 cut(s) 369
BssT1I CCWWGG 2 cut(s) 21, 559
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 5 cut(s) 587, 739, 1473, 1481, 1649
Bst4CI ACNGT 2 cut(s) 187, 929
Bst6I CTCTTC 2 cut(s) 398, 737
BstACI GRCGYC 2 cut(s) 174, 264
BstC8I GCNNGC 9 cut(s) 46, 142, 355, 555, 778, 1050, 1173, 1303, 1531
BstDSI CCRYGG 3 cut(s) 241, 638, 1534
BstENI CCTNNNNNAGG 1 cut(s) 320
BstF5I GGATG 1 cut(s) 1348
BstFNI CGCG 5 cut(s) 164, 357, 404, 596, 640
BstHHI GCGC 3 cut(s) 166, 359, 596
BstKTI GATC 9 cut(s) 128, 301, 541, 1165, 1276, 1471, 1521, 1543, 1618
BstMAI GTCTC 2 cut(s) 1561, 1621
BstMBI GATC 9 cut(s) 125, 298, 538, 1162, 1273, 1468, 1518, 1540, 1615
BstMCI CGRYCG 2 cut(s) 45, 441
BstNI CCWGG 5 cut(s) 587, 739, 1473, 1481, 1649
BstSCI CCNGG 7 cut(s) 452, 453, 585, 737, 1471, 1479, 1647
BstSFI CTRYAG 1 cut(s) 217
BstUI CGCG 5 cut(s) 164, 357, 404, 596, 640
BstV1I GCAGC 7 cut(s) 478, 481, 643, 868, 1225, 1285, 1651
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 3 cut(s) 1273, 1540, 1615
BstYI RGATCY 3 cut(s) 1273, 1540, 1615
BstZI CGGCCG 2 cut(s) 42, 438
BtgI CCRYGG 3 cut(s) 241, 638, 1534
BtgZI GCGATG 1 cut(s) 1070
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 1 cut(s) 1348
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 3 cut(s) 810, 1245, 1726
Cac8I GCNNGC 9 cut(s) 46, 142, 355, 555, 778, 1050, 1173, 1303, 1531
CaiI CAGNNNCTG 2 cut(s) 1619, 1714
CciI TCATGA 1 cut(s) 1416
CfoI GCGC 3 cut(s) 166, 359, 596
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 6 cut(s) 286, 413, 426, 456, 1138, 1588
Cfr42I CCGCGG 1 cut(s) 641
Cfr9I CCCGGG 1 cut(s) 453
CseI GACGC 5 cut(s) 170, 182, 272, 410, 585
Csp6I GTAC 2 cut(s) 865, 1222
CviAII CATG 3 cut(s) 487, 1417, 1466
CviQI GTAC 2 cut(s) 865, 1222
DpnI GATC 9 cut(s) 127, 300, 540, 1164, 1275, 1470, 1520, 1542, 1617
DpnII GATC 9 cut(s) 125, 298, 538, 1162, 1273, 1468, 1518, 1540, 1615
EaeI YGGCCR 6 cut(s) 3, 42, 250, 330, 438, 1698
EagI CGGCCG 2 cut(s) 42, 438
Eam1104I CTCTTC 2 cut(s) 398, 737
EarI CTCTTC 2 cut(s) 398, 737
EciI GGCGGA 2 cut(s) 118, 223
Ecl136II GAGCTC 1 cut(s) 193
EclXI CGGCCG 2 cut(s) 42, 438
Eco130I CCWWGG 2 cut(s) 21, 559
Eco24I GRGCYC 2 cut(s) 195, 376
Eco47I GGWCC 3 cut(s) 413, 1138, 1588
Eco52I CGGCCG 2 cut(s) 42, 438
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 1481
Eco88I CYCGRG 6 cut(s) 290, 363, 409, 445, 453, 1328
EcoICRI GAGCTC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 320
EcoO109I RGGNCCY 4 cut(s) 286, 413, 1138, 1588
EcoRI GAATTC 1 cut(s) 1202
EcoRII CCWGG 5 cut(s) 585, 737, 1471, 1479, 1647
EcoT14I CCWWGG 2 cut(s) 21, 559
EcoT22I ATGCAT 1 cut(s) 920
EcoT38I GRGCYC 2 cut(s) 195, 376
ErhI CCWWGG 2 cut(s) 21, 559
FaeI CATG 3 cut(s) 490, 1420, 1469
FatI CATG 3 cut(s) 486, 1416, 1465
FauI CCCGC 1 cut(s) 362
FokI GGATG 1 cut(s) 1355
FriOI GRGCYC 2 cut(s) 195, 376
FspBI CTAG 6 cut(s) 513, 560, 996, 1142, 1172, 1530
GlaI GCGC 3 cut(s) 165, 358, 595
GsaI CCCAGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 569
HapII CCGG 8 cut(s) 45, 454, 473, 617, 689, 695, 701, 719
HgaI GACGC 5 cut(s) 170, 182, 272, 410, 585
HhaI GCGC 3 cut(s) 166, 359, 596
Hin1I GRCGYC 2 cut(s) 174, 264
Hin1II CATG 3 cut(s) 490, 1420, 1469
Hin6I GCGC 3 cut(s) 164, 357, 594
HinP1I GCGC 3 cut(s) 164, 357, 594
HindIII AAGCTT 2 cut(s) 987, 1434
HinfI GANTC 6 cut(s) 337, 499, 734, 874, 1250, 1264
HpaII CCGG 8 cut(s) 45, 454, 473, 617, 689, 695, 701, 719
HphI GGTGA 2 cut(s) 67, 1705
Hpy166II GTNNAC 1 cut(s) 484
Hpy188III TCNNGA 8 cut(s) 292, 481, 1066, 1417, 1451, 1670, 1708, 1735
Hpy8I GTNNAC 1 cut(s) 484
HpyCH4III ACNGT 2 cut(s) 187, 929
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 7 cut(s) 466, 493, 553, 918, 1356, 1403, 1585
HpySE526I ACGT 1 cut(s) 108
Hsp92I GRCGYC 2 cut(s) 174, 264
Hsp92II CATG 3 cut(s) 490, 1420, 1469
HspAI GCGC 3 cut(s) 164, 357, 594
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
KspI CCGCGG 1 cut(s) 641
Kzo9I GATC 9 cut(s) 125, 298, 538, 1162, 1273, 1468, 1518, 1540, 1615
LmnI GCTCC 7 cut(s) 100, 236, 588, 691, 697, 703, 1183
Lsp1109I GCAGC 7 cut(s) 478, 481, 643, 868, 1225, 1285, 1651
LweI GCATC 4 cut(s) 480, 1093, 1108, 1333
MaeI CTAG 6 cut(s) 513, 560, 996, 1142, 1172, 1530
MaeII ACGT 1 cut(s) 108
MalI GATC 9 cut(s) 127, 300, 540, 1164, 1275, 1470, 1520, 1542, 1617
MbiI CCGCTC 1 cut(s) 965
MboI GATC 9 cut(s) 125, 298, 538, 1162, 1273, 1468, 1518, 1540, 1615
MboII GAAGA 8 cut(s) 193, 385, 682, 694, 754, 808, 866, 1198
MflI RGATCY 3 cut(s) 1273, 1540, 1615
MhlI GDGCHC 4 cut(s) 195, 370, 376, 452
MlsI TGGCCA 1 cut(s) 1700
MluCI AATT 5 cut(s) 1109, 1202, 1556, 1596, 1728
MluI ACGCGT 1 cut(s) 402
MluNI TGGCCA 1 cut(s) 1700
MlyI GAGTC 2 cut(s) 346, 743
MmeI TCCRAC 5 cut(s) 144, 183, 195, 579, 609
Mox20I TGGCCA 1 cut(s) 1700
Mph1103I ATGCAT 1 cut(s) 920
MroNI GCCGGC 1 cut(s) 44
MroXI GAANNNNTTC 2 cut(s) 392, 803
MscI TGGCCA 1 cut(s) 1700
MseI TTAA 4 cut(s) 318, 921, 972, 1740
Msp20I TGGCCA 1 cut(s) 1700
MspA1I CMGCKG 2 cut(s) 640, 656
MspI CCGG 8 cut(s) 45, 454, 473, 617, 689, 695, 701, 719
MspR9I CCNGG 7 cut(s) 454, 455, 587, 739, 1473, 1481, 1649
Mva1269I GAATGC 2 cut(s) 920, 1587
MvaI CCWGG 5 cut(s) 587, 739, 1473, 1481, 1649
MvnI CGCG 5 cut(s) 164, 357, 404, 596, 640
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 2 cut(s) 454, 455
NdeII GATC 9 cut(s) 125, 298, 538, 1162, 1273, 1468, 1518, 1540, 1615
NgoMIV GCCGGC 1 cut(s) 44
NheI GCTAGC 2 cut(s) 1171, 1529
NlaIII CATG 3 cut(s) 490, 1420, 1469
NlaIV GGNNCC 8 cut(s) 232, 414, 693, 699, 705, 1275, 1478, 1590
NmeAIII GCCGAG 4 cut(s) 20, 207, 228, 358
NsiI ATGCAT 1 cut(s) 920
PaeR7I CTCGAG 1 cut(s) 290
PagI TCATGA 1 cut(s) 1416
PcsI WCGNNNNNNNCGW 4 cut(s) 168, 255, 264, 443
PctI GAATGC 2 cut(s) 920, 1587
PdiI GCCGGC 1 cut(s) 46
PdmI GAANNNNTTC 2 cut(s) 392, 803
PfeI GAWTC 4 cut(s) 499, 874, 1250, 1264
PflFI GACNNNGTC 1 cut(s) 206
PflMI CCANNNNNTGG 1 cut(s) 1714
PfoI TCCNGGA 2 cut(s) 737, 1647
PleI GAGTC 2 cut(s) 345, 742
PpsI GAGTC 2 cut(s) 345, 742
PpuMI RGGWCCY 3 cut(s) 413, 1138, 1588
PsiI TTATAA 1 cut(s) 792
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 3 cut(s) 413, 1138, 1588
Psp6I CCWGG 5 cut(s) 585, 737, 1471, 1479, 1647
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 5 cut(s) 585, 737, 1471, 1479, 1647
PspN4I GGNNCC 8 cut(s) 232, 414, 693, 699, 705, 1275, 1478, 1590
PspPI GGNCC 6 cut(s) 286, 413, 426, 456, 1138, 1588
PspPPI RGGWCCY 3 cut(s) 413, 1138, 1588
PstNI CAGNNNCTG 2 cut(s) 1619, 1714
PsuI RGATCY 3 cut(s) 1273, 1540, 1615
PsyI GACNNNGTC 1 cut(s) 206
RsaI GTAC 2 cut(s) 866, 1223
RsaNI GTAC 2 cut(s) 865, 1222
SacI GAGCTC 1 cut(s) 195
SacII CCGCGG 1 cut(s) 641
SaqAI TTAA 4 cut(s) 318, 921, 972, 1740
Sau3AI GATC 9 cut(s) 125, 298, 538, 1162, 1273, 1468, 1518, 1540, 1615
Sau96I GGNCC 6 cut(s) 286, 413, 426, 456, 1138, 1588
SchI GAGTC 2 cut(s) 346, 743
ScrFI CCNGG 7 cut(s) 454, 455, 587, 739, 1473, 1481, 1649
SduI GDGCHC 4 cut(s) 195, 370, 376, 452
SfaNI GCATC 4 cut(s) 480, 1093, 1108, 1333
SfcI CTRYAG 1 cut(s) 217
Sfr274I CTCGAG 1 cut(s) 290
Sfr303I CCGCGG 1 cut(s) 641
SgrBI CCGCGG 1 cut(s) 641
SinI GGWCC 3 cut(s) 413, 1138, 1588
SlaI CTCGAG 1 cut(s) 290
SmaI CCCGGG 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 194, 290
SmoI CTYRAG 2 cut(s) 194, 290
Sse9I AATT 5 cut(s) 1109, 1202, 1556, 1596, 1728
SspMI CTAG 6 cut(s) 513, 560, 996, 1142, 1172, 1530
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 7 cut(s) 452, 453, 585, 737, 1471, 1479, 1647
StyI CCWWGG 2 cut(s) 21, 559
TaaI ACNGT 2 cut(s) 187, 929
TaiI ACGT 1 cut(s) 111
TaqI TCGA 5 cut(s) 189, 258, 291, 807, 1034
TasI AATT 5 cut(s) 1109, 1202, 1556, 1596, 1728
TfiI GAWTC 4 cut(s) 499, 874, 1250, 1264
Tru1I TTAA 4 cut(s) 318, 921, 972, 1740
Tru9I TTAA 4 cut(s) 318, 921, 972, 1740
TscAI CASTG 3 cut(s) 817, 1252, 1726
TseI GCWGC 7 cut(s) 466, 469, 656, 881, 1213, 1298, 1664
TspDTI ATGAA 4 cut(s) 867, 927, 1405, 1569
TspGWI ACGGA 1 cut(s) 615
TspMI CCCGGG 1 cut(s) 453
TspRI CASTG 3 cut(s) 817, 1252, 1726
Tth111I GACNNNGTC 1 cut(s) 206
Van91I CCANNNNNTGG 1 cut(s) 1714
VpaK11BI GGWCC 3 cut(s) 413, 1138, 1588
XagI CCTNNNNNAGG 1 cut(s) 320
XapI RAATTY 2 cut(s) 1202, 1596
XhoI CTCGAG 1 cut(s) 290
XmaI CCCGGG 1 cut(s) 453
XmaJI CCTAGG 1 cut(s) 559
XmnI GAANNNNTTC 2 cut(s) 392, 803
XspI CTAG 6 cut(s) 513, 560, 996, 1142, 1172, 1530
Zsp2I ATGCAT 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.