RLG00000003623
HSP70 Family

hsp70-Hsp90 organizing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011330
Physical Location & Seq
Forward (+)
50887518 .. 50890567
3050 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003623

Sequence Viewer

Length: 1752 bp
ATGGCCGACGAAGCCAAAGCCAAGGGCAACGCCGCCTACTCGGCCGGCGACTACACCGCCGCCATTACCCACTTCACCGAAGCCATCAACCTCGCTCCCACCAACCACGTCCTCTACTCCAACCGATCCGCCTCCTACGCCAACCTCCACAAATACTCCGACGCCCTCTCCGACGCCAAGAAGACCGTCGAGCTCAAGCCCGACTGGGTCAAAGGCTACAGCCGCCTCGGCTCCGCCCACCACGGCCTCGGCCACTTCGACGACGCCGTTTCCGCTTACAAGAAGGGCCTCGAGATTGATCCCAACAACGCCGCCCTTAAAGAGGGCTTGGCAGAGAGTCAGTCCGCCGCCGCCCGCGCCGCCCGAGCACGAGCCCAGCCGCCGTCGAACCTCTTCGGCGACGCGTTCTCGGGTCCTCAGATGTGGGCCAAGCTCACGGCCGACCCGAGTACCCGGGCCTTTATGCAGCAGCCGGATTTCGTTCACATGATGCAGGAGATTCAGAAAAACCCTAGCAATCTCAATCTTTATTTGAAGGACCAGAGGGTTATGCAAGCCCTAGGGGTTTTGCTCAATGTGAAGCTCCAGGGAGGCGCGTCGGAGGACGACGATATGCCGGATTTTCCGTCGGAGAGGACGCGGTCTGCGGAGGCCGCTGCTGAGCCGCAGAAGAAGGAGGAGAAGAAGCCGGAACGGGAGCCGGAGCCGGAGCCGATGGAAGTGCCGGAGGAGGAGAAGGAGGCCAGGAAGAGGAAAGCTGAGGCTTTAAAGGAGAAGGAGGCTGGCAATGCGGCTTATAAGAAGAAGGATTTCGACACTGCTATTCAGCACTATACCAAGGCATTGGAGATAGATGATGAAGATATTTCGTACTTGTTGAATCGTGCTGCGACTTATTTGGAAATGGGTCAGTATGATGAATGCATTAAAGACTGTGACAAGGCTGTGGAAAGGGGAAGAGAGGTAAGGGCGGACTTTAAGATGATAGCAAAAGCTTTGACTAGGAAGGGAACTGCCATTGTGAAAACAGCAAAAACCTCAAAAGACTATGAGCCTGCTATTGAGATATTCCAGAAAGCTCTCACAGAGCATCGCAACCCCGACACCCTAAAGAAATTGAATGATGCTGAGAAAGCTAAGAAGGACCTAGAGCAACAAGAGTATTTTGATCCAAAGCTAGCAGATGAGGAGCGAGAGAAAGGTAATGAATTCTTCAAGCAGCAGAAGTACCCAGAGGCTATTAGGCATTACACTGAATCTTTGAGAAGGAATCCAAAGGATCCAAAGGCATATAGCAATAGAGCTGCTTGCTATACAAAGTTGGGGGCAATGCCTGAGGGATTGAAGGATGCTGAGAAGTGCATTGAGCTAGACCCAACCTTTTCAAAGGGCTATACCAGAAAAGGTGCGGTCCAGTTTTTCATGAGAGAGTATGACAAAGCTTTGGAAACGTATCAAGAAGGTCTGAAGCATGATCCTGGCAACCAGGACTTGCTTGATGGTGTGAGGAGATGTGTAGAGCAGATCAACAAAGCTAGCCGTGGGGATCTGAGTGCTGATGAATTGAAGGAGAGACAGGCTAAGGGAATGCAAGACCCAGAAATTCAGAATATCCTTTCAGATCCTGTTATGAGACAGGTGTTGGTTGATTTCCAGGAAAATCCCAAAGCTGCTCAAGAACACTCAAAGAACCCGATGGTGATGGCCAAAATCCAGAAGCTAGTCAGTGCTGGAATTGTCCAGATTAAGTGA

Protein Analysis

584

Amino Acids

65.58

Weight (kDa)

6.16

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_BSK1_C PF25575 4 - 70 1.2e-06 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 71 - 103 2.8e-07 Tetratricopeptide repeat
TPR_2 PF07719 71 - 103 3.8e-06 Tetratricopeptide repeat
STI1-HOP_DP PF17830 139 - 191 3.8e-22 STI1/HOP, DP domain
TPR_BSK1_C PF25575 252 - 321 5.2e-08 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 262 - 286 6.9e-06 Tetratricopeptide repeat
TPR_11 PF13414 262 - 303 2.1e-07 TPR repeat
TPR_1 PF00515 292 - 317 4e-06 Tetratricopeptide repeat
TPR_12 PF13424 292 - 361 5e-07 Tetratricopeptide repeat
TPR_BSK1_C PF25575 374 - 461 9.9e-06 Serine/threonine-protein kinase BSK1-like, TPR repeats
TPR_1 PF00515 400 - 427 9.5e-06 Tetratricopeptide repeat
TPR_16 PF13432 400 - 451 2.4e-06 Tetratricopeptide repeat
TPR_11 PF13414 401 - 442 1.2e-08 TPR repeat
TPR_1 PF00515 428 - 461 1.2e-06 Tetratricopeptide repeat
TPR_1 PF00515 463 - 495 4.1e-06 Tetratricopeptide repeat
STI1-HOP_DP PF17830 524 - 575 1.6e-15 STI1/HOP, DP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62740 AT4G12400 AT4G12400
fragaria_vesca FvH4_5g00400 FvH4_5g00400 FvH4_5g00400
malus_domestica MD04G1081900.v1.1 MD06G1065400.v1.1
prunus_persica Prupe.5G076500_v2.0.a1
pyrus_communis pycom04g07380 pycom06g05640
rosa_chinensis RchiOBHm_Chr7g0202131
rosa_laevigata RLG00000003623
rosa_multiflora Rmu_ssc0000228.1_g000003
rosa_roxburghii Rroxscaffold_3G00254830
rosa_rugosa Rorug07G0068200 Rorug07G0068300
rosa_samantha Rh7AG195700 Rh7CG206800
rosa_wichuraiana Rw7G017140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 798
AccII CGCG 4 cut(s) 357, 404, 596, 640
AclWI GGATC 8 cut(s) 120, 293, 1163, 1274, 1287, 1469, 1554, 1616
AcoI YGGCCR 5 cut(s) 3, 42, 250, 438, 1704
AcsI RAATTY 2 cut(s) 1208, 1602
AcuI CTGAAG 1 cut(s) 1487
AcyI GRCGYC 3 cut(s) 162, 174, 264
AfaI GTAC 3 cut(s) 451, 872, 1229
AfiI CCNNNNNNNGG 2 cut(s) 322, 750
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 7 cut(s) 535, 880, 1120, 1216, 1345, 1386, 1567
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 5 cut(s) 585, 743, 1477, 1485, 1653
Alw21I GWGCWC 2 cut(s) 195, 370
Alw26I GTCTC 2 cut(s) 1567, 1627
AlwI GGATC 8 cut(s) 120, 293, 1163, 1274, 1287, 1469, 1554, 1616
AlwNI CAGNNNCTG 1 cut(s) 1625
Ama87I CYCGRG 5 cut(s) 290, 363, 409, 445, 453
ApeKI GCWGC 7 cut(s) 466, 469, 656, 887, 1219, 1304, 1670
ApoI RAATTY 2 cut(s) 1208, 1602
Asp700I GAANNNNTTC 2 cut(s) 392, 809
AspA2I CCTAGG 1 cut(s) 559
AspLEI GCGC 2 cut(s) 359, 596
AspS9I GGNCC 7 cut(s) 286, 413, 426, 456, 538, 1144, 1411
AsuC2I CCSGG 2 cut(s) 454, 455
AsuHPI GGTGA 2 cut(s) 67, 1711
AsuNHI GCTAGC 2 cut(s) 1177, 1535
AvaI CYCGRG 5 cut(s) 290, 363, 409, 445, 453
AvaII GGWCC 4 cut(s) 413, 538, 1144, 1411
AvrII CCTAGG 1 cut(s) 559
AxyI CCTNAGG 1 cut(s) 1335
BaeI ACNNNNGTAYC 2 cut(s) 433, 466
BalI TGGCCA 1 cut(s) 1706
BamHI GGATCC 1 cut(s) 1279
BanII GRGCYC 2 cut(s) 195, 376
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 195, 370
BbvCI CCTCAGC 1 cut(s) 759
BbvI GCAGC 7 cut(s) 478, 481, 643, 874, 1231, 1291, 1657
BccI CCATC 5 cut(s) 92, 709, 1493, 1690, 1696
BceAI ACGGC 5 cut(s) 251, 259, 367, 453, 1524
BcgI CGANNNNNNTGC 2 cut(s) 703, 737
BciT130I CCWGG 5 cut(s) 587, 745, 1479, 1487, 1655
BcnI CCSGG 2 cut(s) 454, 455
BcoDI GTCTC 2 cut(s) 1567, 1627
BfaI CTAG 8 cut(s) 513, 560, 1002, 1148, 1178, 1370, 1536, 1721
BfmI CTRYAG 1 cut(s) 217
BglI GCCNNNNNGGC 2 cut(s) 41, 228
BlnI CCTAGG 1 cut(s) 559
BlpI GCTNAGC 1 cut(s) 660
Bme1390I CCNGG 7 cut(s) 454, 455, 587, 745, 1479, 1487, 1655
Bme18I GGWCC 4 cut(s) 413, 538, 1144, 1411
BmeT110I CYCGRG 5 cut(s) 290, 363, 409, 445, 453
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 7 cut(s) 286, 413, 426, 456, 538, 1144, 1411
BmiI GGNNCC 6 cut(s) 232, 414, 699, 705, 711, 1281
BmrFI CCNGG 7 cut(s) 454, 455, 587, 745, 1479, 1487, 1655
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 4 cut(s) 480, 1099, 1114, 1339
BmtI GCTAGC 2 cut(s) 1181, 1539
BmuI ACTGGG 1 cut(s) 214
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 569
Bpu10I CCTNAGC 2 cut(s) 759, 1581
Bpu1102I GCTNAGC 1 cut(s) 660
BpuEI CTTGAG 2 cut(s) 179, 1659
BpuMI CCSGG 2 cut(s) 454, 455
BsaHI GRCGYC 3 cut(s) 162, 174, 264
BsaJI CCNNGG 9 cut(s) 21, 226, 241, 247, 453, 559, 586, 837, 1540
BsaXI ACNNNNNCTCC 4 cut(s) 140, 152, 170, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 750
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 2 cut(s) 209, 1414
Bse21I CCTNAGG 1 cut(s) 1335
Bse3DI GCAATG 2 cut(s) 793, 1335
BseBI CCWGG 5 cut(s) 587, 745, 1479, 1487, 1655
BseDI CCNNGG 9 cut(s) 21, 226, 241, 247, 453, 559, 586, 837, 1540
BseGI GGATG 1 cut(s) 1354
BseLI CCNNNNNNNGG 2 cut(s) 322, 750
BseMI GCAATG 2 cut(s) 793, 1335
BseMII CTCAG 7 cut(s) 431, 651, 750, 1119, 1326, 1344, 1541
BseNI ACTGG 2 cut(s) 209, 1414
BseRI GAGGAG 5 cut(s) 692, 743, 746, 1202, 1522
BseX3I CGGCCG 2 cut(s) 42, 438
BseXI GCAGC 7 cut(s) 478, 481, 643, 874, 1231, 1291, 1657
BseYI CCCAGC 1 cut(s) 375
Bsh1236I CGCG 4 cut(s) 357, 404, 596, 640
Bsh1285I CGRYCG 2 cut(s) 45, 441
BsiEI CGRYCG 2 cut(s) 45, 441
BsiHKAI GWGCWC 2 cut(s) 195, 370
BsiHKCI CYCGRG 5 cut(s) 290, 363, 409, 445, 453
BsiSI CCGG 8 cut(s) 45, 454, 473, 617, 689, 701, 707, 725
BslI CCNNNNNNNGG 2 cut(s) 322, 750
BsmAI GTCTC 2 cut(s) 1567, 1627
BsmI GAATGC 2 cut(s) 926, 1593
BsoBI CYCGRG 5 cut(s) 290, 363, 409, 445, 453
Bsp1286I GDGCHC 3 cut(s) 195, 370, 376
Bsp143I GATC 8 cut(s) 125, 298, 1168, 1279, 1474, 1524, 1546, 1621
Bsp1720I GCTNAGC 1 cut(s) 660
BspCNI CTCAG 7 cut(s) 430, 652, 751, 1120, 1327, 1345, 1542
BspFNI CGCG 4 cut(s) 357, 404, 596, 640
BspHI TCATGA 1 cut(s) 1422
BspLI GGNNCC 6 cut(s) 232, 414, 699, 705, 711, 1281
BspOI GCTAGC 2 cut(s) 1181, 1539
BspPI GGATC 8 cut(s) 120, 293, 1163, 1274, 1287, 1469, 1554, 1616
BsrDI GCAATG 2 cut(s) 793, 1335
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 2 cut(s) 209, 1414
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 9 cut(s) 21, 226, 241, 247, 453, 559, 586, 837, 1540
BssMI GATC 8 cut(s) 125, 298, 1168, 1279, 1474, 1524, 1546, 1621
BssNI GRCGYC 3 cut(s) 162, 174, 264
BssSI CACGAG 1 cut(s) 369
BssT1I CCWWGG 3 cut(s) 21, 559, 837
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 5 cut(s) 587, 745, 1479, 1487, 1655
Bst4CI ACNGT 2 cut(s) 187, 935
Bst6I CTCTTC 3 cut(s) 398, 743, 952
BstACI GRCGYC 3 cut(s) 162, 174, 264
BstC8I GCNNGC 8 cut(s) 46, 355, 555, 784, 1056, 1179, 1309, 1537
BstDEI CTNAG 9 cut(s) 417, 660, 759, 1128, 1137, 1335, 1353, 1550, 1581
BstDSI CCRYGG 2 cut(s) 241, 1540
BstENI CCTNNNNNAGG 1 cut(s) 320
BstF5I GGATG 1 cut(s) 1354
BstFNI CGCG 4 cut(s) 357, 404, 596, 640
BstHHI GCGC 2 cut(s) 359, 596
BstKTI GATC 8 cut(s) 128, 301, 1171, 1282, 1477, 1527, 1549, 1624
BstMAI GTCTC 2 cut(s) 1567, 1627
BstMBI GATC 8 cut(s) 125, 298, 1168, 1279, 1474, 1524, 1546, 1621
BstMCI CGRYCG 2 cut(s) 45, 441
BstNI CCWGG 5 cut(s) 587, 745, 1479, 1487, 1655
BstSCI CCNGG 7 cut(s) 452, 453, 585, 743, 1477, 1485, 1653
BstSFI CTRYAG 1 cut(s) 217
BstUI CGCG 4 cut(s) 357, 404, 596, 640
BstV1I GCAGC 7 cut(s) 478, 481, 643, 874, 1231, 1291, 1657
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 3 cut(s) 1279, 1546, 1621
BstXI CCANNNNNNTGG 1 cut(s) 844
BstYI RGATCY 3 cut(s) 1279, 1546, 1621
BstZI CGGCCG 2 cut(s) 42, 438
Bsu36I CCTNAGG 1 cut(s) 1335
BtgI CCRYGG 2 cut(s) 241, 1540
BtgZI GCGATG 1 cut(s) 1076
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 1 cut(s) 1354
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 3 cut(s) 816, 1251, 1732
Cac8I GCNNGC 8 cut(s) 46, 355, 555, 784, 1056, 1179, 1309, 1537
CaiI CAGNNNCTG 1 cut(s) 1625
CciI TCATGA 1 cut(s) 1422
CfoI GCGC 2 cut(s) 359, 596
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 7 cut(s) 286, 413, 426, 456, 538, 1144, 1411
Cfr9I CCCGGG 1 cut(s) 453
CseI GACGC 6 cut(s) 170, 182, 272, 410, 585, 646
Csp6I GTAC 3 cut(s) 450, 871, 1228
CviAII CATG 3 cut(s) 487, 1423, 1472
CviQI GTAC 3 cut(s) 450, 871, 1228
DdeI CTNAG 9 cut(s) 417, 660, 759, 1128, 1137, 1335, 1353, 1550, 1581
DpnI GATC 8 cut(s) 127, 300, 1170, 1281, 1476, 1526, 1548, 1623
DpnII GATC 8 cut(s) 125, 298, 1168, 1279, 1474, 1524, 1546, 1621
DraI TTTAAA 1 cut(s) 768
EaeI YGGCCR 5 cut(s) 3, 42, 250, 438, 1704
EagI CGGCCG 2 cut(s) 42, 438
Eam1104I CTCTTC 3 cut(s) 398, 743, 952
EarI CTCTTC 3 cut(s) 398, 743, 952
EciI GGCGGA 4 cut(s) 118, 223, 334, 986
Ecl136II GAGCTC 1 cut(s) 193
EclXI CGGCCG 2 cut(s) 42, 438
Eco130I CCWWGG 3 cut(s) 21, 559, 837
Eco24I GRGCYC 2 cut(s) 195, 376
Eco47I GGWCC 4 cut(s) 413, 538, 1144, 1411
Eco52I CGGCCG 2 cut(s) 42, 438
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 1487
Eco81I CCTNAGG 1 cut(s) 1335
Eco88I CYCGRG 5 cut(s) 290, 363, 409, 445, 453
EcoICRI GAGCTC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 320
EcoO109I RGGNCCY 3 cut(s) 286, 413, 1144
EcoRI GAATTC 1 cut(s) 1208
EcoRII CCWGG 5 cut(s) 585, 743, 1477, 1485, 1653
EcoT14I CCWWGG 3 cut(s) 21, 559, 837
EcoT22I ATGCAT 1 cut(s) 926
EcoT38I GRGCYC 2 cut(s) 195, 376
ErhI CCWWGG 3 cut(s) 21, 559, 837
FaeI CATG 3 cut(s) 490, 1426, 1475
FatI CATG 3 cut(s) 486, 1422, 1471
FauI CCCGC 1 cut(s) 362
FokI GGATG 1 cut(s) 1361
FriOI GRGCYC 2 cut(s) 195, 376
FspBI CTAG 8 cut(s) 513, 560, 1002, 1148, 1178, 1370, 1536, 1721
GlaI GCGC 2 cut(s) 358, 595
GsaI CCCAGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 569
HapII CCGG 8 cut(s) 45, 454, 473, 617, 689, 701, 707, 725
HgaI GACGC 6 cut(s) 170, 182, 272, 410, 585, 646
HhaI GCGC 2 cut(s) 359, 596
Hin1I GRCGYC 3 cut(s) 162, 174, 264
Hin1II CATG 3 cut(s) 490, 1426, 1475
Hin6I GCGC 2 cut(s) 357, 594
HinP1I GCGC 2 cut(s) 357, 594
HindIII AAGCTT 2 cut(s) 993, 1440
HinfI GANTC 5 cut(s) 337, 499, 880, 1256, 1270
HpaII CCGG 8 cut(s) 45, 454, 473, 617, 689, 701, 707, 725
HphI GGTGA 2 cut(s) 67, 1711
Hpy166II GTNNAC 1 cut(s) 484
Hpy188III TCNNGA 7 cut(s) 292, 1072, 1423, 1457, 1676, 1714, 1741
Hpy8I GTNNAC 1 cut(s) 484
HpyCH4III ACNGT 2 cut(s) 187, 935
HpyCH4IV ACGT 2 cut(s) 108, 1451
HpyCH4V TGCA 6 cut(s) 466, 493, 553, 924, 1362, 1591
HpyF3I CTNAG 9 cut(s) 417, 660, 759, 1128, 1137, 1335, 1353, 1550, 1581
HpySE526I ACGT 2 cut(s) 108, 1451
Hsp92I GRCGYC 3 cut(s) 162, 174, 264
Hsp92II CATG 3 cut(s) 490, 1426, 1475
HspAI GCGC 2 cut(s) 357, 594
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Kzo9I GATC 8 cut(s) 125, 298, 1168, 1279, 1474, 1524, 1546, 1621
LmnI GCTCC 7 cut(s) 100, 236, 588, 697, 703, 709, 1189
Lsp1109I GCAGC 7 cut(s) 478, 481, 643, 874, 1231, 1291, 1657
LweI GCATC 4 cut(s) 480, 1099, 1114, 1339
MaeI CTAG 8 cut(s) 513, 560, 1002, 1148, 1178, 1370, 1536, 1721
MaeII ACGT 2 cut(s) 108, 1451
MaeIII GTNAC 1 cut(s) 935
MalI GATC 8 cut(s) 127, 300, 1170, 1281, 1476, 1526, 1548, 1623
MboI GATC 8 cut(s) 125, 298, 1168, 1279, 1474, 1524, 1546, 1621
MboII GAAGA 9 cut(s) 193, 385, 682, 694, 760, 814, 872, 969, 1204
MflI RGATCY 3 cut(s) 1279, 1546, 1621
MhlI GDGCHC 3 cut(s) 195, 370, 376
MlsI TGGCCA 1 cut(s) 1706
MluCI AATT 5 cut(s) 1115, 1208, 1562, 1602, 1734
MluI ACGCGT 1 cut(s) 402
MluNI TGGCCA 1 cut(s) 1706
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 5 cut(s) 144, 183, 195, 579, 609
Mox20I TGGCCA 1 cut(s) 1706
Mph1103I ATGCAT 1 cut(s) 926
MroNI GCCGGC 1 cut(s) 44
MroXI GAANNNNTTC 2 cut(s) 392, 809
MscI TGGCCA 1 cut(s) 1706
MseI TTAA 5 cut(s) 318, 767, 927, 978, 1746
Msp20I TGGCCA 1 cut(s) 1706
MspA1I CMGCKG 1 cut(s) 656
MspI CCGG 8 cut(s) 45, 454, 473, 617, 689, 701, 707, 725
MspR9I CCNGG 7 cut(s) 454, 455, 587, 745, 1479, 1487, 1655
Mva1269I GAATGC 2 cut(s) 926, 1593
MvaI CCWGG 5 cut(s) 587, 745, 1479, 1487, 1655
MvnI CGCG 4 cut(s) 357, 404, 596, 640
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 2 cut(s) 454, 455
NdeII GATC 8 cut(s) 125, 298, 1168, 1279, 1474, 1524, 1546, 1621
NgoMIV GCCGGC 1 cut(s) 44
NheI GCTAGC 2 cut(s) 1177, 1535
NlaIII CATG 3 cut(s) 490, 1426, 1475
NlaIV GGNNCC 6 cut(s) 232, 414, 699, 705, 711, 1281
NmeAIII GCCGAG 3 cut(s) 20, 207, 228
NmuCI GTSAC 1 cut(s) 935
NsiI ATGCAT 1 cut(s) 926
PaeR7I CTCGAG 1 cut(s) 290
PagI TCATGA 1 cut(s) 1422
PcsI WCGNNNNNNNCGW 4 cut(s) 168, 255, 264, 443
PctI GAATGC 2 cut(s) 926, 1593
PdiI GCCGGC 1 cut(s) 46
PdmI GAANNNNTTC 2 cut(s) 392, 809
PfeI GAWTC 4 cut(s) 499, 880, 1256, 1270
PflFI GACNNNGTC 2 cut(s) 206, 640
PfoI TCCNGGA 1 cut(s) 1653
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
PpuMI RGGWCCY 2 cut(s) 413, 1144
PsiI TTATAA 1 cut(s) 798
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 2 cut(s) 413, 1144
Psp6I CCWGG 5 cut(s) 585, 743, 1477, 1485, 1653
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 5 cut(s) 585, 743, 1477, 1485, 1653
PspN4I GGNNCC 6 cut(s) 232, 414, 699, 705, 711, 1281
PspPI GGNCC 7 cut(s) 286, 413, 426, 456, 538, 1144, 1411
PspPPI RGGWCCY 2 cut(s) 413, 1144
PstNI CAGNNNCTG 1 cut(s) 1625
PsuI RGATCY 3 cut(s) 1279, 1546, 1621
PsyI GACNNNGTC 2 cut(s) 206, 640
RsaI GTAC 3 cut(s) 451, 872, 1229
RsaNI GTAC 3 cut(s) 450, 871, 1228
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 5 cut(s) 318, 767, 927, 978, 1746
Sau3AI GATC 8 cut(s) 125, 298, 1168, 1279, 1474, 1524, 1546, 1621
Sau96I GGNCC 7 cut(s) 286, 413, 426, 456, 538, 1144, 1411
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 7 cut(s) 454, 455, 587, 745, 1479, 1487, 1655
SduI GDGCHC 3 cut(s) 195, 370, 376
SfaNI GCATC 4 cut(s) 480, 1099, 1114, 1339
SfcI CTRYAG 1 cut(s) 217
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 4 cut(s) 413, 538, 1144, 1411
SlaI CTCGAG 1 cut(s) 290
SmaI CCCGGG 1 cut(s) 455
SmlI CTYRAG 3 cut(s) 194, 290, 1674
SmoI CTYRAG 3 cut(s) 194, 290, 1674
Sse9I AATT 5 cut(s) 1115, 1208, 1562, 1602, 1734
SspMI CTAG 8 cut(s) 513, 560, 1002, 1148, 1178, 1370, 1536, 1721
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 7 cut(s) 452, 453, 585, 743, 1477, 1485, 1653
StyI CCWWGG 3 cut(s) 21, 559, 837
TaaI ACNGT 2 cut(s) 187, 935
TaiI ACGT 2 cut(s) 111, 1454
TaqI TCGA 5 cut(s) 189, 258, 291, 386, 813
TasI AATT 5 cut(s) 1115, 1208, 1562, 1602, 1734
TfiI GAWTC 4 cut(s) 499, 880, 1256, 1270
Tru1I TTAA 5 cut(s) 318, 767, 927, 978, 1746
Tru9I TTAA 5 cut(s) 318, 767, 927, 978, 1746
TscAI CASTG 3 cut(s) 823, 1258, 1732
TseFI GTSAC 1 cut(s) 935
TseI GCWGC 7 cut(s) 466, 469, 656, 887, 1219, 1304, 1670
Tsp45I GTSAC 1 cut(s) 935
TspDTI ATGAA 5 cut(s) 873, 933, 1221, 1411, 1575
TspGWI ACGGA 1 cut(s) 615
TspMI CCCGGG 1 cut(s) 453
TspRI CASTG 3 cut(s) 823, 1258, 1732
Tth111I GACNNNGTC 2 cut(s) 206, 640
VpaK11BI GGWCC 4 cut(s) 413, 538, 1144, 1411
XagI CCTNNNNNAGG 1 cut(s) 320
XapI RAATTY 2 cut(s) 1208, 1602
XhoI CTCGAG 1 cut(s) 290
XmaI CCCGGG 1 cut(s) 453
XmaJI CCTAGG 1 cut(s) 559
XmnI GAANNNNTTC 2 cut(s) 392, 809
XspI CTAG 8 cut(s) 513, 560, 1002, 1148, 1178, 1370, 1536, 1721
Zsp2I ATGCAT 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.