AT4G12840

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003514
Physical Location & Seq
Reverse (-)
7533439 .. 7536266
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12840.2

Sequence Viewer

Length: 1188 bp
ATGGATCTTCTTTATTCGTTGCCTTTTCAGGGATCATTAAGTATGAATCAAAAACGAAAGGGCTTGTCTCTTCGTCGCTTGTTCTCTTTAGTGTTTTTTCTTGCAATGATCTTCCTTATAGGGAATGCTTTTATTACAGTAGACTATAAAGAGGGTATCGCGGGATGGAGTTCGATAATTAGACTAAATCTTGCTAAACTAAAGATGTGCAAGACACAACTTAGGCCTCCTGGTAGCGAGACGTTACCGCGAGGTATTGTTGCAAGTACATCTGACTTGGAAATGCGACCGTTATGGGGAGCAAAGCGCGATAAGAAACCGAAACCAAGCTTATTAGCTATGGCAGTTGGAATAAGACAAAAGGAAAGTGTCAACAAGATTGTCAAGAAGTTTCCATCAAGTGAGTTTGTTGTGATGCTGTTTCACTACGATGGTGCTGTGGATGAATGGAAGGAGTTTGAGTGGAGTGACACTGCCATTCATATTTCTGTAGTAAACCAAACCAAATGGTGGTTTGCAAAGCGTTTTCTACACCCGGATATTGTCTCAGCTTATTCTTACATTTTTCTATGGGATGAAGATCTCGGTGTTGACCATTTTGATGCTAGAAGGTATGTTTCAATTATTAAAGAAGAAAAGCTTGAAATATCACAACCTGCTCTTGATCCCAATTTCTCTGAAGTGCATCATCAACTTACCTCACGAGACAAGAAATCAAGAGTACATAGGAGAACATACAAAGTCATTGGTCGGGCGAGGTGCAACGAGAATAGCACTGGACCTCCTTGCACAGGATTTGTTGAAATGATGGCTCCTGTGTTTTCCAGAGCAGCTTGGAGGTGTACATGGCATATGATTCAGAATGACTTAAACCATGGATGGGGTATAGATTTTCAGCTTGGATATTGTGCACAGGGTGATCGAACCAAAAATATCGGTATTGTTGATTCCGAGTATATACTTCATATGGGTCTTCCTACATTAGGAGGAGGTTCAGCTGAAAACAAGACGGATTCAGGGAAGCTCGATAAGACCAAGACTCCGCATGCTGCAGACAAATCATCATCAGTGTCAACTGGTAGAACTGAGGTTCGGAAGCAAACTTATGTCGAGTTAGAGACTTTTAAACATAGATGGAAAAATGCTGTGAAGAACGATGAGTGCTGGATAGACCGATTCCAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

395

Amino Acids

45.28

Weight (kDa)

9.36

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 74 - 384 5.8e-145 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 664
AccB7I CCANNNNNTGG 1 cut(s) 510
AccI GTMKAC 1 cut(s) 141
AccII CGCG 3 cut(s) 161, 250, 309
AciI CCGC 3 cut(s) 161, 248, 1043
AclWI GGATC 3 cut(s) 12, 40, 659
AcuI CTGAAG 1 cut(s) 699
AdeI CACNNNGTG 1 cut(s) 917
AfaI GTAC 3 cut(s) 268, 723, 844
AfiI CCNNNNNNNGG 6 cut(s) 29, 296, 510, 791, 880, 983
AgsI TTSAA 3 cut(s) 621, 644, 803
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 8 cut(s) 330, 338, 551, 640, 833, 898, 998, 1024
AluI AGCT 8 cut(s) 330, 338, 551, 640, 833, 898, 998, 1024
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 5 cut(s) 72, 233, 550, 699, 1112
Alw44I GTGCAC 1 cut(s) 909
AlwI GGATC 3 cut(s) 12, 40, 659
AoxI GGCC 1 cut(s) 224
ApaLI GTGCAC 1 cut(s) 909
ApeKI GCWGC 2 cut(s) 830, 1049
AspLEI GCGC 1 cut(s) 309
AspS9I GGNCC 1 cut(s) 779
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 1 cut(s) 929
AvaII GGWCC 1 cut(s) 779
BaeGI GKGCMC 1 cut(s) 913
BauI CACGAG 1 cut(s) 702
BbsI GAAGAC 1 cut(s) 965
Bbv12I GWGCWC 1 cut(s) 913
BbvI GCAGC 2 cut(s) 842, 1036
BccI CCATC 6 cut(s) 159, 403, 425, 802, 873, 1128
BciT130I CCWGG 1 cut(s) 231
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 5 cut(s) 72, 233, 550, 699, 1112
BfaI CTAG 1 cut(s) 606
BfmI CTRYAG 2 cut(s) 489, 1050
BfuAI ACCTGC 1 cut(s) 664
BglII AGATCT 1 cut(s) 580
BisI GCNGC 2 cut(s) 831, 1050
BlsI GCNGC 2 cut(s) 832, 1051
Bme1390I CCNGG 2 cut(s) 231, 536
Bme18I GGWCC 1 cut(s) 779
BmgT120I GGNCC 1 cut(s) 779
BmiI GGNNCC 1 cut(s) 813
BmrFI CCNGG 2 cut(s) 231, 536
BmsI GCATC 3 cut(s) 405, 592, 694
BpiI GAAGAC 1 cut(s) 965
BpuMI CCSGG 1 cut(s) 536
BsaBI GATNNNNATC 1 cut(s) 579
BsaJI CCNNGG 1 cut(s) 874
BsaXI ACNNNNNCTCC 6 cut(s) 446, 476, 766, 796, 1024, 1054
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 296, 510, 791, 880, 983
Bse1I ACTGG 2 cut(s) 781, 1081
Bse3DI GCAATG 1 cut(s) 111
Bse8I GATNNNNATC 1 cut(s) 579
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 874
BseGI GGATG 4 cut(s) 170, 448, 580, 884
BseJI GATNNNNATC 1 cut(s) 579
BseLI CCNNNNNNNGG 6 cut(s) 29, 296, 510, 791, 880, 983
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 561, 1077
BseNI ACTGG 2 cut(s) 781, 1081
BseRI GAGGAG 1 cut(s) 1002
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 2 cut(s) 842, 1036
Bsh1236I CGCG 3 cut(s) 161, 250, 309
Bsh1285I CGRYCG 1 cut(s) 290
BshFI GGCC 1 cut(s) 226
BsiEI CGRYCG 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 913
BsiSI CCGG 1 cut(s) 536
BslI CCNNNNNNNGG 6 cut(s) 29, 296, 510, 791, 880, 983
BsmAI GTCTC 5 cut(s) 72, 233, 550, 699, 1112
BsmBI CGTCTC 1 cut(s) 233
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 913
Bsp1407I TGTACA 1 cut(s) 842
Bsp143I GATC 6 cut(s) 4, 32, 108, 580, 664, 919
Bsp19I CCATGG 1 cut(s) 874
BspACI CCGC 3 cut(s) 161, 248, 1043
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 2 cut(s) 560, 1078
BspFNI CGCG 3 cut(s) 161, 250, 309
BspLI GGNNCC 1 cut(s) 813
BspMAI CTGCAG 1 cut(s) 1054
BspMI ACCTGC 1 cut(s) 664
BspPI GGATC 3 cut(s) 12, 40, 659
BsrDI GCAATG 1 cut(s) 111
BsrGI TGTACA 1 cut(s) 842
BsrI ACTGG 2 cut(s) 781, 1081
BssECI CCNNGG 1 cut(s) 874
BssMI GATC 6 cut(s) 4, 32, 108, 580, 664, 919
BssSI CACGAG 1 cut(s) 702
BssT1I CCWWGG 1 cut(s) 874
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 2 cut(s) 139, 291
Bst6I CTCTTC 1 cut(s) 75
BstAUI TGTACA 1 cut(s) 842
BstC8I GCNNGC 1 cut(s) 1047
BstDEI CTNAG 3 cut(s) 221, 547, 1086
BstDSI CCRYGG 1 cut(s) 874
BstENI CCTNNNNNAGG 2 cut(s) 789, 981
BstF5I GGATG 4 cut(s) 170, 448, 580, 884
BstFNI CGCG 3 cut(s) 161, 250, 309
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 6 cut(s) 7, 35, 111, 583, 667, 922
BstMAI GTCTC 5 cut(s) 72, 233, 550, 699, 1112
BstMBI GATC 6 cut(s) 4, 32, 108, 580, 664, 919
BstMCI CGRYCG 1 cut(s) 290
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 1049
BstSCI CCNGG 2 cut(s) 229, 534
BstSFI CTRYAG 2 cut(s) 489, 1050
BstSLI GKGCMC 1 cut(s) 913
BstUI CGCG 3 cut(s) 161, 250, 309
BstV1I GCAGC 2 cut(s) 842, 1036
BstV2I GAAGAC 1 cut(s) 965
BstX2I RGATCY 2 cut(s) 4, 580
BstYI RGATCY 2 cut(s) 4, 580
BsuRI GGCC 1 cut(s) 226
BtgI CCRYGG 1 cut(s) 874
BtsCI GGATG 4 cut(s) 170, 448, 580, 884
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 471, 774, 1074
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 1 cut(s) 1047
CfoI GCGC 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 779
Csp6I GTAC 3 cut(s) 267, 722, 843
CviAII CATG 4 cut(s) 846, 875, 1046, 1185
CviQI GTAC 3 cut(s) 267, 722, 843
DdeI CTNAG 3 cut(s) 221, 547, 1086
DpnI GATC 6 cut(s) 6, 34, 110, 582, 666, 921
DpnII GATC 6 cut(s) 4, 32, 108, 580, 664, 919
DraI TTTAAA 1 cut(s) 1126
DraIII CACNNNGTG 1 cut(s) 917
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 874
Eco147I AGGCCT 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 779
Eco57I CTGAAG 1 cut(s) 699
EcoNI CCTNNNNNAGG 2 cut(s) 789, 981
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 874
ErhI CCWWGG 1 cut(s) 874
Esp3I CGTCTC 1 cut(s) 233
FaeI CATG 4 cut(s) 849, 878, 1049, 1188
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FatI CATG 4 cut(s) 845, 874, 1045, 1184
FauI CCCGC 1 cut(s) 154
FauNDI CATATG 2 cut(s) 852, 966
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 2 cut(s) 831, 1050
FokI GGATG 4 cut(s) 177, 455, 587, 891
Fsp4HI GCNGC 2 cut(s) 831, 1050
FspBI CTAG 1 cut(s) 606
GlaI GCGC 1 cut(s) 308
GluI GCNGC 2 cut(s) 831, 1050
HaeIII GGCC 1 cut(s) 226
HapII CCGG 1 cut(s) 536
HhaI GCGC 1 cut(s) 309
Hin1II CATG 4 cut(s) 849, 878, 1049, 1188
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HincII GTYRAC 3 cut(s) 373, 592, 1074
HindII GTYRAC 3 cut(s) 373, 592, 1074
HindIII AAGCTT 2 cut(s) 328, 638
HinfI GANTC 6 cut(s) 46, 856, 947, 1013, 1039, 1176
HpaII CCGG 1 cut(s) 536
HphI GGTGA 1 cut(s) 929
Hpy166II GTNNAC 7 cut(s) 142, 373, 496, 592, 843, 911, 1074
Hpy188I TCNGA 5 cut(s) 274, 679, 861, 952, 1095
Hpy188III TCNNGA 5 cut(s) 385, 662, 702, 717, 825
Hpy8I GTNNAC 7 cut(s) 142, 373, 496, 592, 843, 911, 1074
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 2 cut(s) 445, 603
HpyCH4III ACNGT 2 cut(s) 139, 291
HpyCH4IV ACGT 1 cut(s) 242
HpyCH4V TGCA 9 cut(s) 104, 210, 263, 518, 685, 762, 789, 911, 1052
HpyF3I CTNAG 3 cut(s) 221, 547, 1086
HpySE526I ACGT 1 cut(s) 242
Hsp92II CATG 4 cut(s) 849, 878, 1049, 1188
HspAI GCGC 1 cut(s) 307
Kzo9I GATC 6 cut(s) 4, 32, 108, 580, 664, 919
LmnI GCTCC 2 cut(s) 299, 817
Lsp1109I GCAGC 2 cut(s) 842, 1036
LweI GCATC 3 cut(s) 405, 592, 694
MaeI CTAG 1 cut(s) 606
MaeII ACGT 1 cut(s) 242
MaeIII GTNAC 2 cut(s) 243, 467
MalI GATC 6 cut(s) 6, 34, 110, 582, 666, 921
MboI GATC 6 cut(s) 4, 32, 108, 580, 664, 919
MboII GAAGA 6 cut(s) 62, 103, 590, 644, 965, 1162
MflI RGATCY 2 cut(s) 4, 580
MhlI GDGCHC 1 cut(s) 913
MluCI AATT 3 cut(s) 177, 621, 670
MlyI GAGTC 1 cut(s) 1033
MmeI TCCRAC 1 cut(s) 328
MseI TTAA 4 cut(s) 38, 627, 869, 1125
MslI CAYNNNNRTG 2 cut(s) 429, 600
MspA1I CMGCKG 1 cut(s) 998
MspI CCGG 1 cut(s) 536
MspR9I CCNGG 2 cut(s) 231, 536
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 3 cut(s) 161, 250, 309
NciI CCSGG 1 cut(s) 536
NcoI CCATGG 1 cut(s) 874
NdeI CATATG 2 cut(s) 852, 966
NdeII GATC 6 cut(s) 4, 32, 108, 580, 664, 919
NlaIII CATG 4 cut(s) 849, 878, 1049, 1188
NlaIV GGNNCC 1 cut(s) 813
NmuCI GTSAC 1 cut(s) 467
NspI RCATGY 1 cut(s) 1049
PaeI GCATGC 1 cut(s) 1049
PceI AGGCCT 1 cut(s) 226
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 5 cut(s) 46, 856, 947, 1013, 1176
PflMI CCANNNNNTGG 1 cut(s) 510
PkrI GCNGC 2 cut(s) 832, 1051
PleI GAGTC 1 cut(s) 1033
PpsI GAGTC 1 cut(s) 1033
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 1 cut(s) 813
PspPI GGNCC 1 cut(s) 779
PstI CTGCAG 1 cut(s) 1054
PsuI RGATCY 2 cut(s) 4, 580
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 3 cut(s) 268, 723, 844
RsaNI GTAC 3 cut(s) 267, 722, 843
RseI CAYNNNNRTG 2 cut(s) 429, 600
SaqAI TTAA 4 cut(s) 38, 627, 869, 1125
SatI GCNGC 2 cut(s) 831, 1050
Sau3AI GATC 6 cut(s) 4, 32, 108, 580, 664, 919
Sau96I GGNCC 1 cut(s) 779
SchI GAGTC 1 cut(s) 1033
ScrFI CCNGG 2 cut(s) 231, 536
SduI GDGCHC 1 cut(s) 913
SfaNI GCATC 3 cut(s) 405, 592, 694
SfcI CTRYAG 2 cut(s) 489, 1050
SinI GGWCC 1 cut(s) 779
SmiMI CAYNNNNRTG 2 cut(s) 429, 600
SphI GCATGC 1 cut(s) 1049
Sse9I AATT 3 cut(s) 177, 621, 670
SseBI AGGCCT 1 cut(s) 226
SsiI CCGC 3 cut(s) 161, 248, 1043
SspMI CTAG 1 cut(s) 606
StuI AGGCCT 1 cut(s) 226
StyD4I CCNGG 2 cut(s) 229, 534
StyI CCWWGG 1 cut(s) 874
TaaI ACNGT 2 cut(s) 139, 291
TaiI ACGT 1 cut(s) 245
TaqI TCGA 4 cut(s) 173, 922, 1026, 1110
TaqII GACCGA 1 cut(s) 1188
TasI AATT 3 cut(s) 177, 621, 670
TatI WGTACW 3 cut(s) 266, 721, 842
TfiI GAWTC 5 cut(s) 46, 856, 947, 1013, 1176
Tru1I TTAA 4 cut(s) 38, 627, 869, 1125
Tru9I TTAA 4 cut(s) 38, 627, 869, 1125
TscAI CASTG 3 cut(s) 478, 781, 1074
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 2 cut(s) 830, 1049
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 5 cut(s) 59, 459, 470, 591, 953
TspGWI ACGGA 1 cut(s) 1025
TspRI CASTG 3 cut(s) 478, 781, 1074
Van91I CCANNNNNTGG 1 cut(s) 510
VneI GTGCAC 1 cut(s) 909
VpaK11BI GGWCC 1 cut(s) 779
XagI CCTNNNNNAGG 2 cut(s) 789, 981
XceI RCATGY 1 cut(s) 1049
XmiI GTMKAC 1 cut(s) 141
XspI CTAG 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.