Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003514

Physical Location & Seq

Genomic Coordinates Reverse (-)

28450797 .. 28454300

Gene Structure 3504 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1154100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGGTTTCCAAGACCTTTGACTTGCTAATGCAACCCTTTATGGGGTCCCTAAAACAAAAAGGCAGCTCGAAGGATATGAACTCAAAGTCATCAACAGACTTACTGGCCATTGCAGTTGAATTAAAGCAAAAGAAAAGTGTGAACAAGATTGTAAAGAAGAGGGGCTTGAGATATCACAGCCAGCACTTGATCCTGCCAACTCCGAGGTGCATCTTCTTATGGCGAGAGACAACAGAACAGAAGTACACAGACTCTAATGATTCTTTCTCAGTAAATTCTTCACTACTTGCTCCGATCTTACGTGTTCTTCACCACTCTTTTGGTTGGTGGTGCATTAACAGGAACATAAACAAGACACTTCGTGGTGGAAGGAAGTGTGATGAAAACAGCACAGATCCTTCATGCACAGGGTTTGTAGAAATGATGGCTCCTGTTTATTCAAGAGCATCCTGGCGCTGCATATGGCATCTTATTCAGTCCAAGTTCTGGAGCCAGCGGAGTACTTCAAGCAGCGATTCGGATATTGATACTGATAGAGAATCTGAAACTGCAGTTGTTACTGAGATTTCCACCGAAGGAAGCTGCTACAGAATAGATGTCAGTGATGCCTCTGATGACAAGGAGGATGATCCGAAGCGTGTTCTTAAATCAGCAAAAGTCAAGCACTTTGATGAGATGAATGCTACGTTTGACAAATTAAAGAATGCTGCTAAGAATGTCAATGATTGGATCAGCATGGAGGATTGCTTTGAGAAACTTAATAAGCAGCTTGAGAAGGTGATGCGCATGACCAAGAAGGAAAAAGTACCGTCTCTCTACATCAAAGCGCTTGTTTTGCTGGAGGGCATTTTGGCTGAAGCGCTGGAAAAAAAGGCTGCTAAGAAGAAGATGACTAGCAGCAATGCCAAGGCCTTGACCGTGATGAAACAGAAACTGAAGAAGAACAATAGGCAGTATGAGGACTTGATTAGTTTGTTTAGGGAGAATCCCGTGAAGAGCGAGGAGGATTCTGATGAGTCTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005852 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0019538 GO:0031369 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

PCI eIF-3c_N

Protein Analysis

342

Amino Acids

39.05

Weight (kDa)

9.15

Isoelectric Point (pI)

46.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF707	PF05212	120 - 163	1.6e-16	Protein of unknown function (DUF707)
eIF-3c_N	PF05470	201 - 339	6.7e-38	Eukaryotic translation initiation factor 3 subunit 8 N-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12840 AT4G12840 AT4G12840
fragaria_vesca	FvH4_2g17510 FvH4_2g17510 FvH4_2g17510 FvH4_2g17510 FvH4_2g17510 FvH4_2g17510 FvH4_2g17510 FvH4_2g17510 FvH4_2g17530 FvH4_2g17530 FvH4_2g17530 FvH4_2g17530 FvH4_2g17530
malus_domestica	MD05G1154100.v1.1 MD10G1148400.v1.1
prunus_persica	Prupe.8G187400_v2.0.a1 Prupe.8G187400_v2.0.a1 Prupe.8G187400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0282241
rosa_laevigata	RLG00000012898
rosa_multiflora	Rmu_sc0014549.1_g000001
rosa_roxburghii	Rroxscaffold_7G00186370
rosa_rugosa	Rorug02G0256100 Rorug03G0287100 Rorug03G0287200 Rorug06G0146600 Rorug07G0302200
rosa_samantha	Rh6AG259700 Rh6BG263000 Rh6CG261900 Rh6DG253900
rosa_wichuraiana	Rw6G022470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	785
AccB7I	CCANNNNNTGG	1 cut(s)	486
AciI	CCGC	1 cut(s)	496
AclWI	GGATC	4 cut(s)	184, 389, 623, 737
AcoI	YGGCCR	1 cut(s)	105
AcsI	RAATTY	1 cut(s)	274
AcuI	CTGAAG	2 cut(s)	876, 956
AdeI	CACNNNGTG	1 cut(s)	362
AfaI	GTAC	3 cut(s)	245, 502, 807
AfeI	AGCGCT	2 cut(s)	828, 861
AfiI	CCNNNNNNNGG	3 cut(s)	41, 42, 486
AflIII	ACRYGT	1 cut(s)	301
AgsI	TTSAA	3 cut(s)	119, 441, 507
AjnI	CCWGG	1 cut(s)	449
AluBI	AGCT	3 cut(s)	66, 582, 769
AluI	AGCT	3 cut(s)	66, 582, 769
Alw26I	GTCTC	2 cut(s)	221, 816
AlwI	GGATC	4 cut(s)	184, 389, 623, 737
AlwNI	CAGNNNCTG	1 cut(s)	934
Aor51HI	AGCGCT	2 cut(s)	828, 861
AoxI	GGCC	2 cut(s)	105, 909
ApeKI	GCWGC	8 cut(s)	63, 456, 510, 582, 707, 766, 875, 897
ApoI	RAATTY	1 cut(s)	274
AspLEI	GCGC	4 cut(s)	456, 786, 829, 862
AspS9I	GGNCC	1 cut(s)	45
AsuHPI	GGTGA	2 cut(s)	302, 790
AvaII	GGWCC	1 cut(s)	45
BalI	TGGCCA	1 cut(s)	107
BbsI	GAAGAC	1 cut(s)	1011
BbvI	GCAGC	8 cut(s)	75, 443, 522, 569, 694, 778, 862, 909
BccI	CCATC	1 cut(s)	418
BciT130I	CCWGG	1 cut(s)	451
BcoDI	GTCTC	2 cut(s)	221, 816
BfaI	CTAG	1 cut(s)	894
BfmI	CTRYAG	2 cut(s)	549, 586
BfoI	RGCGCY	3 cut(s)	457, 830, 863
BisI	GCNGC	8 cut(s)	64, 457, 511, 583, 708, 767, 876, 898
BlsI	GCNGC	8 cut(s)	65, 458, 512, 584, 709, 768, 877, 899
BmcAI	AGTACT	1 cut(s)	502
Bme1390I	CCNGG	1 cut(s)	451
Bme18I	GGWCC	1 cut(s)	45
BmgT120I	GGNCC	1 cut(s)	45
BmiI	GGNNCC	4 cut(s)	46, 47, 429, 491
BmrFI	CCNGG	1 cut(s)	451
BmsI	GCATC	5 cut(s)	219, 455, 475, 595, 771
BpiI	GAAGAC	1 cut(s)	1011
BpmI	CTGGAG	2 cut(s)	508, 860
BpuEI	CTTGAG	2 cut(s)	187, 791
BsaAI	YACGTR	1 cut(s)	302
BsaJI	CCNNGG	2 cut(s)	203, 906
Bsc4I	CCNNNNNNNGG	3 cut(s)	41, 42, 486
Bse1I	ACTGG	1 cut(s)	108
Bse3DI	GCAATG	2 cut(s)	108, 907
BseBI	CCWGG	1 cut(s)	451
BseDI	CCNNGG	2 cut(s)	203, 906
BseGI	GGATG	2 cut(s)	446, 631
BseLI	CCNNNNNNNGG	3 cut(s)	41, 42, 486
BseMI	GCAATG	2 cut(s)	108, 907
BseMII	CTCAG	2 cut(s)	282, 552
BseNI	ACTGG	1 cut(s)	108
BseRI	GAGGAG	1 cut(s)	1016
BseXI	GCAGC	8 cut(s)	75, 443, 522, 569, 694, 778, 862, 909
BshFI	GGCC	2 cut(s)	107, 911
BslFI	GGGAC	1 cut(s)	31
BslI	CCNNNNNNNGG	3 cut(s)	41, 42, 486
BsmAI	GTCTC	2 cut(s)	221, 816
BsmBI	CGTCTC	1 cut(s)	816
BsmFI	GGGAC	1 cut(s)	31
BsmI	GAATGC	2 cut(s)	685, 709
BsnI	GGCC	2 cut(s)	107, 911
Bsp143I	GATC	5 cut(s)	189, 294, 394, 628, 729
BspACI	CCGC	1 cut(s)	496
BspANI	GGCC	2 cut(s)	107, 911
BspCNI	CTCAG	2 cut(s)	281, 553
BspLI	GGNNCC	4 cut(s)	46, 47, 429, 491
BspMAI	CTGCAG	1 cut(s)	553
BspPI	GGATC	4 cut(s)	184, 389, 623, 737
BspQI	GCTCTTC	1 cut(s)	989
BsrDI	GCAATG	2 cut(s)	108, 907
BsrI	ACTGG	1 cut(s)	108
BssECI	CCNNGG	2 cut(s)	203, 906
BssMI	GATC	5 cut(s)	189, 294, 394, 628, 729
BssT1I	CCWWGG	1 cut(s)	906
Bst2UI	CCWGG	1 cut(s)	451
Bst4CI	ACNGT	2 cut(s)	810, 919
Bst6I	CTCTTC	2 cut(s)	152, 989
BstBAI	YACGTR	1 cut(s)	302
BstC8I	GCNNGC	2 cut(s)	182, 494
BstDEI	CTNAG	4 cut(s)	268, 561, 711, 879
BstF5I	GGATG	2 cut(s)	446, 631
BstH2I	RGCGCY	3 cut(s)	457, 830, 863
BstHHI	GCGC	4 cut(s)	456, 786, 829, 862
BstKTI	GATC	5 cut(s)	192, 297, 397, 631, 732
BstMAI	GTCTC	2 cut(s)	221, 816
BstMBI	GATC	5 cut(s)	189, 294, 394, 628, 729
BstMWI	GCNNNNNNNGC	1 cut(s)	835
BstNI	CCWGG	1 cut(s)	451
BstSCI	CCNGG	1 cut(s)	449
BstSFI	CTRYAG	2 cut(s)	549, 586
BstV1I	GCAGC	8 cut(s)	75, 443, 522, 569, 694, 778, 862, 909
BstV2I	GAAGAC	1 cut(s)	1011
BstX2I	RGATCY	1 cut(s)	394
BstXI	CCANNNNNNTGG	1 cut(s)	320
BstYI	RGATCY	1 cut(s)	394
BsuRI	GGCC	2 cut(s)	107, 911
BtsCI	GGATG	2 cut(s)	446, 631
BtsIMutI	CAGTG	1 cut(s)	607
Cac8I	GCNNGC	2 cut(s)	182, 494
CaiI	CAGNNNCTG	1 cut(s)	934
CfoI	GCGC	4 cut(s)	456, 786, 829, 862
Cfr13I	GGNCC	1 cut(s)	45
Csp6I	GTAC	3 cut(s)	244, 501, 806
CviAII	CATG	3 cut(s)	402, 736, 787
CviQI	GTAC	3 cut(s)	244, 501, 806
DdeI	CTNAG	4 cut(s)	268, 561, 711, 879
DpnI	GATC	5 cut(s)	191, 296, 396, 630, 731
DpnII	GATC	5 cut(s)	189, 294, 394, 628, 729
DraIII	CACNNNGTG	1 cut(s)	362
EaeI	YGGCCR	1 cut(s)	105
Eam1104I	CTCTTC	2 cut(s)	152, 989
EarI	CTCTTC	2 cut(s)	152, 989
Eco130I	CCWWGG	1 cut(s)	906
Eco147I	AGGCCT	1 cut(s)	911
Eco32I	GATATC	1 cut(s)	173
Eco47I	GGWCC	1 cut(s)	45
Eco47III	AGCGCT	2 cut(s)	828, 861
Eco57I	CTGAAG	2 cut(s)	876, 956
EcoO109I	RGGNCCY	1 cut(s)	45
EcoRII	CCWGG	1 cut(s)	449
EcoRV	GATATC	1 cut(s)	173
EcoT14I	CCWWGG	1 cut(s)	906
ErhI	CCWWGG	1 cut(s)	906
Esp3I	CGTCTC	1 cut(s)	816
FaeI	CATG	3 cut(s)	405, 739, 790
FalI	AAGNNNNNCTT	2 cut(s)	149, 181
FaqI	GGGAC	1 cut(s)	31
FatI	CATG	3 cut(s)	401, 735, 786
FauNDI	CATATG	1 cut(s)	461
Fnu4HI	GCNGC	8 cut(s)	64, 457, 511, 583, 708, 767, 876, 898
FokI	GGATG	2 cut(s)	433, 638
Fsp4HI	GCNGC	8 cut(s)	64, 457, 511, 583, 708, 767, 876, 898
FspAI	RTGCGCAY	1 cut(s)	785
FspBI	CTAG	1 cut(s)	894
FspI	TGCGCA	1 cut(s)	785
GlaI	GCGC	4 cut(s)	455, 785, 828, 861
GluI	GCNGC	8 cut(s)	64, 457, 511, 583, 708, 767, 876, 898
GsuI	CTGGAG	2 cut(s)	508, 860
HaeII	RGCGCY	3 cut(s)	457, 830, 863
HaeIII	GGCC	2 cut(s)	107, 911
HhaI	GCGC	4 cut(s)	456, 786, 829, 862
Hin1II	CATG	3 cut(s)	405, 739, 790
Hin6I	GCGC	4 cut(s)	454, 784, 827, 860
HinP1I	GCGC	4 cut(s)	454, 784, 827, 860
HinfI	GANTC	7 cut(s)	251, 260, 515, 539, 985, 1007, 1016
HphI	GGTGA	2 cut(s)	302, 790
Hpy166II	GTNNAC	2 cut(s)	142, 246
Hpy188I	TCNGA	7 cut(s)	204, 294, 520, 544, 613, 633, 1012
Hpy188III	TCNNGA	2 cut(s)	441, 487
Hpy8I	GTNNAC	2 cut(s)	142, 246
HpyAV	CCTTC	6 cut(s)	64, 363, 408, 569, 769, 790
HpyCH4III	ACNGT	2 cut(s)	810, 919
HpyCH4IV	ACGT	2 cut(s)	301, 686
HpyCH4V	TGCA	7 cut(s)	31, 113, 210, 333, 405, 459, 551
HpyF10VI	GCNNNNNNNGC	1 cut(s)	835
HpyF3I	CTNAG	4 cut(s)	268, 561, 711, 879
HpySE526I	ACGT	2 cut(s)	301, 686
Hsp92II	CATG	3 cut(s)	405, 739, 790
HspAI	GCGC	4 cut(s)	454, 784, 827, 860
KflI	GGGWCCC	1 cut(s)	45
Kzo9I	GATC	5 cut(s)	189, 294, 394, 628, 729
LguI	GCTCTTC	1 cut(s)	989
LmnI	GCTCC	3 cut(s)	295, 433, 489
Lsp1109I	GCAGC	8 cut(s)	75, 443, 522, 569, 694, 778, 862, 909
LweI	GCATC	5 cut(s)	219, 455, 475, 595, 771
MaeI	CTAG	1 cut(s)	894
MaeII	ACGT	2 cut(s)	301, 686
MaeIII	GTNAC	1 cut(s)	556
MalI	GATC	5 cut(s)	191, 296, 396, 630, 731
MboI	GATC	5 cut(s)	189, 294, 394, 628, 729
MflI	RGATCY	1 cut(s)	394
MlsI	TGGCCA	1 cut(s)	107
MluCI	AATT	3 cut(s)	119, 274, 695
MluNI	TGGCCA	1 cut(s)	107
MlyI	GAGTC	2 cut(s)	245, 1025
MnlI	CCTC	9 cut(s)	153, 198, 616, 619, 733, 835, 952, 994, 997
Mox20I	TGGCCA	1 cut(s)	107
MscI	TGGCCA	1 cut(s)	107
MseI	TTAA	6 cut(s)	122, 336, 645, 698, 759, 1024
MslI	CAYNNNNRTG	1 cut(s)	669
Msp20I	TGGCCA	1 cut(s)	107
MspA1I	CMGCKG	1 cut(s)	496
MspR9I	CCNGG	1 cut(s)	451
Mva1269I	GAATGC	2 cut(s)	685, 709
MvaI	CCWGG	1 cut(s)	451
MwoI	GCNNNNNNNGC	1 cut(s)	835
NdeI	CATATG	1 cut(s)	461
NdeII	GATC	5 cut(s)	189, 294, 394, 628, 729
NlaIII	CATG	3 cut(s)	405, 739, 790
NlaIV	GGNNCC	4 cut(s)	46, 47, 429, 491
NsbI	TGCGCA	1 cut(s)	785
PceI	AGGCCT	1 cut(s)	911
PciSI	GCTCTTC	1 cut(s)	989
PctI	GAATGC	2 cut(s)	685, 709
PfeI	GAWTC	5 cut(s)	260, 515, 539, 985, 1007
PflMI	CCANNNNNTGG	1 cut(s)	486
PkrI	GCNGC	8 cut(s)	65, 458, 512, 584, 709, 768, 877, 899
PleI	GAGTC	2 cut(s)	245, 1024
PpsI	GAGTC	2 cut(s)	245, 1024
Ppu21I	YACGTR	1 cut(s)	302
PpuMI	RGGWCCY	1 cut(s)	45
Psp5II	RGGWCCY	1 cut(s)	45
Psp6I	CCWGG	1 cut(s)	449
PspGI	CCWGG	1 cut(s)	449
PspN4I	GGNNCC	4 cut(s)	46, 47, 429, 491
PspPI	GGNCC	1 cut(s)	45
PspPPI	RGGWCCY	1 cut(s)	45
PstI	CTGCAG	1 cut(s)	553
PstNI	CAGNNNCTG	1 cut(s)	934
PsuI	RGATCY	1 cut(s)	394
RsaI	GTAC	3 cut(s)	245, 502, 807
RsaNI	GTAC	3 cut(s)	244, 501, 806
RseI	CAYNNNNRTG	1 cut(s)	669
SapI	GCTCTTC	1 cut(s)	989
SaqAI	TTAA	6 cut(s)	122, 336, 645, 698, 759, 1024
SatI	GCNGC	8 cut(s)	64, 457, 511, 583, 708, 767, 876, 898
Sau3AI	GATC	5 cut(s)	189, 294, 394, 628, 729
Sau96I	GGNCC	1 cut(s)	45
ScaI	AGTACT	1 cut(s)	502
SchI	GAGTC	2 cut(s)	245, 1025
ScrFI	CCNGG	1 cut(s)	451
SetI	ASST	8 cut(s)	17, 68, 209, 304, 584, 689, 771, 780
SfaNI	GCATC	5 cut(s)	219, 455, 475, 595, 771
SfcI	CTRYAG	2 cut(s)	549, 586
SinI	GGWCC	1 cut(s)	45
SmiMI	CAYNNNNRTG	1 cut(s)	669
SmlI	CTYRAG	2 cut(s)	166, 770
SmoI	CTYRAG	2 cut(s)	166, 770
Sse9I	AATT	3 cut(s)	119, 274, 695
SseBI	AGGCCT	1 cut(s)	911
SsiI	CCGC	1 cut(s)	496
SspMI	CTAG	1 cut(s)	894
StuI	AGGCCT	1 cut(s)	911
StyD4I	CCNGG	1 cut(s)	449
StyI	CCWWGG	1 cut(s)	906
TaaI	ACNGT	2 cut(s)	810, 919
TaiI	ACGT	2 cut(s)	304, 689
TaqI	TCGA	1 cut(s)	68
TasI	AATT	3 cut(s)	119, 274, 695
TatI	WGTACW	2 cut(s)	243, 500
TfiI	GAWTC	5 cut(s)	260, 515, 539, 985, 1007
Tru1I	TTAA	6 cut(s)	122, 336, 645, 698, 759, 1024
Tru9I	TTAA	6 cut(s)	122, 336, 645, 698, 759, 1024
TscAI	CASTG	1 cut(s)	607
TseI	GCWGC	8 cut(s)	63, 456, 510, 582, 707, 766, 875, 897
TspDTI	ATGAA	5 cut(s)	92, 390, 396, 692, 938
TspRI	CASTG	1 cut(s)	607
Van91I	CCANNNNNTGG	1 cut(s)	486
VpaK11BI	GGWCC	1 cut(s)	45
XapI	RAATTY	1 cut(s)	274
XspI	CTAG	1 cut(s)	894
ZrmI	AGTACT	1 cut(s)	502

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.