MD10G1148400.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003514
Physical Location & Seq
Reverse (-)
23589944 .. 23593294
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1148400.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGATGATGCTGAAGACCCCAACTTCTATTTCTCACGAGGACCCAAAGAGGAGGTTCTTTAGTTGCAGTTTCCTTTCTACCATTGCTATGCTTTCTGCAGTTCTCTTCTTTGGGAGCCAATTTGTTGTCACTGATTATAAAGAGAAGTTTTTAGCATGGGGATCGGTTTATGGTATGCAACATACAATTTCGAAAAGTTGCGAGACTCAATGCAGGCCTGAAGGAACTGAGGCACTACCTAAAGGAATTGTTTCCAAGACCTCCGACTTGCTAATGCAACCGTTATGGGGTCCCCGAAACAAAAAGAACACAAAGTCATCAACAAACTTATTGGCCATGGCAGTTGGATTAAAACAAAAGGAAAGTGTGAACAAGATTGTAAAGAAGTTCATATCAAGTGATTTCGTTTTTATGCTTTTCCATTACGACGGTCATGTAAACGAGTGGAGAGATTTGGAATGGAGTGGTAAGGCCATTCATGTGTCCGCTATGAATCAAACTAAGTGGTGGTATGCCAAGCGTTTCTTGCATCCAGATATTGTTTCTGAGTACGAATTCATCTTCCTCTGGGACGAGGACCTTGGAATCGAAAATTTCAATGCTGGAAGATACTTATCCATTATTAGAAAAGAGGGGCTTCAGATATCACAACCAGCACTAGATCCTGACAACTCTGAGGTGCATCATCTTCTTACGGCGAGAGACAACAGAACAGAAGTACACAGGAAGATAAACAAGACGCTTCGTGGTGGAAGGAAGTGTGATGAAAACAGCACAGATCCTCCATGCACAGGGTTTGTAGAAATGATGGCTCCTGTTTTTTCACAAGCATCGTGGCGCTGCATATGGCATATGATTCAGAATGACTTAGTTCATGCTTGGGGCTTGGATTTCCAGCTTGGTTATTGTGCACAGGGTGATCGAACTAAAAATATCGGAATTGTTGATTCTGAGTACATTATTCATTATGGTATTCCTTCATTGGGGGGTTCGGCAGCAAACAAGACAAATGCTGAAGCAAATGATCAACCTGCTCAAAATATAAACTTGCCTAGTTCAGAACAGCCGGTATCTGACCCTAGAACCGAGGTGAGGAAGCTATCCTTGGCTGAATTGGAAACTTTCAAGAACAGATGGAAAAAGGCAATCAGAGAAGATAATTGCTGGATTGATCCATTCCAAAAGAACATCACAGCTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

401

Amino Acids

45.74

Weight (kDa)

8.3

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 70 - 384 2.8e-143 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 1039
AciI CCGC 1 cut(s) 486
AclWI GGATC 4 cut(s) 169, 656, 773, 1166
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 2 cut(s) 554, 592
AcuI CTGAAG 4 cut(s) 32, 240, 623, 1035
AdeI CACNNNGTG 1 cut(s) 917
AfaI GTAC 3 cut(s) 551, 720, 956
AfiI CCNNNNNNNGG 4 cut(s) 287, 791, 983, 1092
AgsI TTSAA 2 cut(s) 598, 1126
AjuI GAANNNNNNNTTGG 4 cut(s) 13, 45, 1088, 1120
AluBI AGCT 3 cut(s) 898, 1099, 1196
AluI AGCT 3 cut(s) 898, 1099, 1196
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 2 cut(s) 197, 696
Alw44I GTGCAC 1 cut(s) 909
AlwI GGATC 4 cut(s) 169, 656, 773, 1166
AoxI GGCC 3 cut(s) 215, 333, 471
ApaLI GTGCAC 1 cut(s) 909
ApeKI GCWGC 2 cut(s) 840, 995
ApoI RAATTY 2 cut(s) 554, 592
Asp700I GAANNNNTTC 1 cut(s) 250
AspLEI GCGC 1 cut(s) 840
AspS9I GGNCC 3 cut(s) 40, 290, 577
AsuHPI GGTGA 2 cut(s) 929, 1102
AsuII TTCGAA 1 cut(s) 191
AvaII GGWCC 3 cut(s) 40, 290, 577
BaeGI GKGCMC 1 cut(s) 913
BalI TGGCCA 1 cut(s) 335
BauI CACGAG 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 913
BbvI GCAGC 2 cut(s) 827, 1007
BccI CCATC 2 cut(s) 802, 1128
BceAI ACGGC 1 cut(s) 711
BcgI CGANNNNNNTGC 2 cut(s) 144, 178
BclI TGATCA 1 cut(s) 1024
BcoDI GTCTC 2 cut(s) 197, 696
BfaI CTAG 3 cut(s) 659, 1053, 1080
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 841
BfuAI ACCTGC 1 cut(s) 1039
BisI GCNGC 2 cut(s) 841, 996
BlsI GCNGC 2 cut(s) 842, 997
Bme18I GGWCC 3 cut(s) 40, 290, 577
BmgT120I GGNCC 3 cut(s) 40, 290, 577
BmiI GGNNCC 5 cut(s) 42, 116, 291, 292, 813
BmsI GCATC 3 cut(s) 538, 691, 839
BpiI GAAGAC 1 cut(s) 20
Bpu14I TTCGAA 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 613
BsaJI CCNNGG 4 cut(s) 336, 580, 1086, 1104
BsaXI ACNNNNNCTCC 2 cut(s) 766, 796
Bsc4I CCNNNNNNNGG 4 cut(s) 287, 791, 983, 1092
Bse118I RCCGGY 1 cut(s) 1066
Bse3DI GCAATG 1 cut(s) 81
Bse8I GATNNNNATC 1 cut(s) 613
BseDI CCNNGG 4 cut(s) 336, 580, 1086, 1104
BseGI GGATG 1 cut(s) 529
BseJI GATNNNNATC 1 cut(s) 613
BseLI CCNNNNNNNGG 4 cut(s) 287, 791, 983, 1092
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 4 cut(s) 219, 537, 666, 942
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 2 cut(s) 827, 1007
BshFI GGCC 3 cut(s) 217, 335, 473
BsiHKAI GWGCWC 1 cut(s) 913
BsiSI CCGG 1 cut(s) 1067
BslFI GGGAC 2 cut(s) 276, 584
BslI CCNNNNNNNGG 4 cut(s) 287, 791, 983, 1092
BsmAI GTCTC 2 cut(s) 197, 696
BsmFI GGGAC 2 cut(s) 276, 584
BsnI GGCC 3 cut(s) 217, 335, 473
Bsp119I TTCGAA 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 913
Bsp143I GATC 6 cut(s) 161, 661, 778, 919, 1024, 1171
Bsp19I CCATGG 1 cut(s) 336
BspACI CCGC 1 cut(s) 486
BspANI GGCC 3 cut(s) 217, 335, 473
BspCNI CTCAG 4 cut(s) 220, 538, 667, 943
BspLI GGNNCC 5 cut(s) 42, 116, 291, 292, 813
BspMAI CTGCAG 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 1039
BspPI GGATC 4 cut(s) 169, 656, 773, 1166
BspT104I TTCGAA 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 81
BsrFI RCCGGY 1 cut(s) 1066
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 4 cut(s) 336, 580, 1086, 1104
BssMI GATC 6 cut(s) 161, 661, 778, 919, 1024, 1171
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 3 cut(s) 336, 580, 1104
Bst2BI CACGAG 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 282, 431
Bst6I CTCTTC 1 cut(s) 110
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 7 cut(s) 228, 501, 546, 675, 868, 951, 1197
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 529
BstH2I RGCGCY 1 cut(s) 841
BstHHI GCGC 1 cut(s) 840
BstKTI GATC 6 cut(s) 164, 664, 781, 922, 1027, 1174
BstMAI GTCTC 2 cut(s) 197, 696
BstMBI GATC 6 cut(s) 161, 661, 778, 919, 1024, 1171
BstMWI GCNNNNNNNGC 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 96
BstSLI GKGCMC 1 cut(s) 913
BstV1I GCAGC 2 cut(s) 827, 1007
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 2 cut(s) 661, 778
BstYI RGATCY 2 cut(s) 661, 778
BsuRI GGCC 3 cut(s) 217, 335, 473
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 129
BveI ACCTGC 1 cut(s) 1039
Cac8I GCNNGC 1 cut(s) 215
CfoI GCGC 1 cut(s) 840
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 3 cut(s) 40, 290, 577
CseI GACGC 1 cut(s) 748
Csp6I GTAC 3 cut(s) 550, 719, 955
CspCI CAANNNNNGTGG 4 cut(s) 485, 520, 815, 850
CviAII CATG 6 cut(s) 156, 337, 434, 479, 786, 875
CviQI GTAC 3 cut(s) 550, 719, 955
DdeI CTNAG 7 cut(s) 228, 501, 546, 675, 868, 951, 1197
DpnI GATC 6 cut(s) 163, 663, 780, 921, 1026, 1173
DpnII GATC 6 cut(s) 161, 661, 778, 919, 1024, 1171
DraIII CACNNNGTG 1 cut(s) 917
EaeI YGGCCR 1 cut(s) 333
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco130I CCWWGG 3 cut(s) 336, 580, 1104
Eco147I AGGCCT 1 cut(s) 217
Eco32I GATATC 1 cut(s) 645
Eco47I GGWCC 3 cut(s) 40, 290, 577
Eco57I CTGAAG 4 cut(s) 32, 240, 623, 1035
EcoO109I RGGNCCY 3 cut(s) 40, 290, 577
EcoRI GAATTC 1 cut(s) 554
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 3 cut(s) 336, 580, 1104
ErhI CCWWGG 3 cut(s) 336, 580, 1104
FaeI CATG 6 cut(s) 159, 340, 437, 482, 789, 878
FalI AAGNNNNNCTT 6 cut(s) 509, 541, 621, 653, 1088, 1120
FaqI GGGAC 2 cut(s) 276, 584
FatI CATG 6 cut(s) 155, 336, 433, 478, 785, 874
FauNDI CATATG 2 cut(s) 845, 852
FbaI TGATCA 1 cut(s) 1024
Fnu4HI GCNGC 2 cut(s) 841, 996
FokI GGATG 1 cut(s) 516
Fsp4HI GCNGC 2 cut(s) 841, 996
FspBI CTAG 3 cut(s) 659, 1053, 1080
GlaI GCGC 1 cut(s) 839
GluI GCNGC 2 cut(s) 841, 996
HaeII RGCGCY 1 cut(s) 841
HaeIII GGCC 3 cut(s) 217, 335, 473
HapII CCGG 1 cut(s) 1067
HgaI GACGC 1 cut(s) 748
HhaI GCGC 1 cut(s) 840
Hin1II CATG 6 cut(s) 159, 340, 437, 482, 789, 878
Hin6I GCGC 1 cut(s) 838
HinP1I GCGC 1 cut(s) 838
HinfI GANTC 5 cut(s) 205, 493, 585, 856, 947
HpaII CCGG 1 cut(s) 1067
HphI GGTGA 2 cut(s) 929, 1102
Hpy166II GTNNAC 4 cut(s) 370, 439, 721, 911
Hpy188III TCNNGA 4 cut(s) 35, 533, 665, 1126
Hpy8I GTNNAC 4 cut(s) 370, 439, 721, 911
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 3 cut(s) 215, 747, 987
HpyCH4III ACNGT 2 cut(s) 282, 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 526
HpyF3I CTNAG 7 cut(s) 228, 501, 546, 675, 868, 951, 1197
Hsp92II CATG 6 cut(s) 159, 340, 437, 482, 789, 878
HspAI GCGC 1 cut(s) 838
KflI GGGWCCC 1 cut(s) 290
Ksp22I TGATCA 1 cut(s) 1024
Kzo9I GATC 6 cut(s) 161, 661, 778, 919, 1024, 1171
LmnI GCTCC 2 cut(s) 114, 817
Lsp1109I GCAGC 2 cut(s) 827, 1007
LweI GCATC 3 cut(s) 538, 691, 839
MaeI CTAG 3 cut(s) 659, 1053, 1080
MaeIII GTNAC 1 cut(s) 127
MalI GATC 6 cut(s) 163, 663, 780, 921, 1026, 1173
MboI GATC 6 cut(s) 161, 661, 778, 919, 1024, 1171
MboII GAAGA 7 cut(s) 25, 97, 553, 618, 680, 739, 1166
MflI RGATCY 2 cut(s) 661, 778
MhlI GDGCHC 1 cut(s) 913
MlsI TGGCCA 1 cut(s) 335
MluCI AATT 8 cut(s) 119, 186, 246, 554, 592, 939, 1112, 1159
MluNI TGGCCA 1 cut(s) 335
MlyI GAGTC 1 cut(s) 199
MmeI TCCRAC 2 cut(s) 288, 325
Mox20I TGGCCA 1 cut(s) 335
MroXI GAANNNNTTC 1 cut(s) 250
MscI TGGCCA 1 cut(s) 335
MseI TTAA 1 cut(s) 350
MslI CAYNNNNRTG 2 cut(s) 86, 479
Msp20I TGGCCA 1 cut(s) 335
MspI CCGG 1 cut(s) 1067
MwoI GCNNNNNNNGC 1 cut(s) 526
NcoI CCATGG 1 cut(s) 336
NdeI CATATG 2 cut(s) 845, 852
NdeII GATC 6 cut(s) 161, 661, 778, 919, 1024, 1171
NlaIII CATG 6 cut(s) 159, 340, 437, 482, 789, 878
NlaIV GGNNCC 5 cut(s) 42, 116, 291, 292, 813
NmuCI GTSAC 1 cut(s) 127
NspV TTCGAA 1 cut(s) 191
PceI AGGCCT 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 250
PfeI GAWTC 4 cut(s) 493, 585, 856, 947
PkrI GCNGC 2 cut(s) 842, 997
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PpuMI RGGWCCY 3 cut(s) 40, 290, 577
PsiI TTATAA 1 cut(s) 138
Psp5II RGGWCCY 3 cut(s) 40, 290, 577
PspN4I GGNNCC 5 cut(s) 42, 116, 291, 292, 813
PspPI GGNCC 3 cut(s) 40, 290, 577
PspPPI RGGWCCY 3 cut(s) 40, 290, 577
PstI CTGCAG 1 cut(s) 100
PsuI RGATCY 2 cut(s) 661, 778
RsaI GTAC 3 cut(s) 551, 720, 956
RsaNI GTAC 3 cut(s) 550, 719, 955
RseI CAYNNNNRTG 2 cut(s) 86, 479
SaqAI TTAA 1 cut(s) 350
SatI GCNGC 2 cut(s) 841, 996
Sau3AI GATC 6 cut(s) 161, 661, 778, 919, 1024, 1171
Sau96I GGNCC 3 cut(s) 40, 290, 577
SchI GAGTC 1 cut(s) 199
SduI GDGCHC 1 cut(s) 913
SfaNI GCATC 3 cut(s) 538, 691, 839
SfcI CTRYAG 1 cut(s) 96
SfuI TTCGAA 1 cut(s) 191
SinI GGWCC 3 cut(s) 40, 290, 577
SmiMI CAYNNNNRTG 2 cut(s) 86, 479
Sse9I AATT 8 cut(s) 119, 186, 246, 554, 592, 939, 1112, 1159
SseBI AGGCCT 1 cut(s) 217
SsiI CCGC 1 cut(s) 486
SspMI CTAG 3 cut(s) 659, 1053, 1080
StuI AGGCCT 1 cut(s) 217
StyI CCWWGG 3 cut(s) 336, 580, 1104
TaaI ACNGT 2 cut(s) 282, 431
TaqI TCGA 3 cut(s) 191, 588, 922
TasI AATT 8 cut(s) 119, 186, 246, 554, 592, 939, 1112, 1159
TatI WGTACW 2 cut(s) 718, 954
TfiI GAWTC 4 cut(s) 493, 585, 856, 947
Tru1I TTAA 1 cut(s) 350
Tru9I TTAA 1 cut(s) 350
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 2 cut(s) 840, 995
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 8 cut(s) 379, 467, 506, 547, 780, 863, 953, 969
TspRI CASTG 1 cut(s) 136
VneI GTGCAC 1 cut(s) 909
VpaK11BI GGWCC 3 cut(s) 40, 290, 577
XapI RAATTY 2 cut(s) 554, 592
XmnI GAANNNNTTC 1 cut(s) 250
XspI CTAG 3 cut(s) 659, 1053, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.