AT4G12860

Calmodulin-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012876
Physical Location & Seq
Reverse (-)
7538444 .. 7538902
459 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12860.1

Sequence Viewer

Length: 459 bp
ATGGATCGTGGAGAATTGAGTAGAGTGTTCCAAATGTTCGATAAGAATGGAGACGGGAAGATTGCGAAAAACGAGCTGAAAGACTTCTTTAAAAGCGTGGGAATCATGGTCCCTGAAAATGAAATCAATGAGATGATAGCAAAGATGGATGTGAATGGAGACGGGGCCATGGATATCGATGAGTTTGGATCATTGTATCAAGAAATGGTGGAAGAGAAAGAGGAAGAAGAGGACATGAGAGAAGCCTTTAGAGTATTTGATCAGAATGGTGATGGGTTCATTACAGATGAAGAGCTGAGGTCGGTGCTCGCGTCAATGGGGTTGAAGCAAGGGAGAACACTTGAAGATTGCAAGAAGATGATAAGTAAGGTTGATGTTGATGGAGATGGTATGGTTAATTTTAAGGAGTTCAAACAAATGATGAGAGGTGGTGGATTTGCTGCTCTTAGCTCCAATTAA

Protein Analysis

152

Amino Acids

17.18

Weight (kDa)

4.43

Isoelectric Point (pI)

27.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 5 - 32 2.8e-07 EF hand domain
EF-hand_6 PF13405 5 - 34 1.3e-07 EF-hand domain
EF-hand_7 PF13499 6 - 66 6e-14 EF-hand domain pair
EF-hand_7 PF13499 76 - 142 6.6e-17 EF-hand domain pair
EF-hand_6 PF13405 78 - 107 1.4e-08 EF-hand domain
EF-hand_1 PF00036 79 - 106 2.1e-08 EF hand domain
EF-hand_5 PF13202 80 - 103 1.8e-07 EF hand
EF-hand_8 PF13833 94 - 142 7.5e-07 EF-hand domain pair
EF-hand_1 PF00036 116 - 142 1.6e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07490 AT4G12860
fragaria_vesca FvH4_2g17620
malus_domestica MD05G1156600.v1.1 MD10G1150400.v1.1
prunus_persica Prupe.8G188800_v2.0.a1
pyrus_communis pycom05g14920 pycom10g12960
rosa_chinensis RchiOBHm_Chr6g0282601
rosa_laevigata RLG00000012882
rosa_multiflora Rmu_co8242345.1_g000002 Rmu_sc0000283.1_g000002 Rmu_sc0005697.1_g000002
rosa_roxburghii Rroxscaffold_7G00186230
rosa_rugosa Rorug06G0148000
rosa_samantha Rh6BG264300 Rh6CG263400 Rh6DG255400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 311
AclWI GGATC 2 cut(s) 12, 196
AgsI TTSAA 3 cut(s) 325, 344, 412
AluBI AGCT 3 cut(s) 76, 295, 450
AluI AGCT 3 cut(s) 76, 295, 450
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 2 cut(s) 45, 153
AlwI GGATC 2 cut(s) 12, 196
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 440
Asp700I GAANNNNTTC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 109, 165
AsuHPI GGTGA 1 cut(s) 281
AvaII GGWCC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 1 cut(s) 427
BccI CCATC 4 cut(s) 139, 266, 374, 380
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 2 cut(s) 45, 153
BisI GCNGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 442
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 109, 165
BmiI GGNNCC 2 cut(s) 111, 166
Bpu10I CCTNAGC 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 287
BseXI GCAGC 1 cut(s) 427
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 167
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 309
BslFI GGGAC 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 45, 153
BsmBI CGTCTC 2 cut(s) 45, 153
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 3 cut(s) 4, 188, 259
Bsp19I CCATGG 1 cut(s) 168
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 1 cut(s) 288
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 111, 166
BspPI GGATC 2 cut(s) 12, 196
BspQI GCTCTTC 1 cut(s) 285
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 3 cut(s) 4, 188, 259
BssT1I CCWWGG 1 cut(s) 168
Bst6I CTCTTC 3 cut(s) 207, 222, 285
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 2 cut(s) 296, 446
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 154
BstFNI CGCG 1 cut(s) 311
BstKTI GATC 3 cut(s) 7, 191, 262
BstMAI GTCTC 2 cut(s) 45, 153
BstMBI GATC 3 cut(s) 4, 188, 259
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 1 cut(s) 167
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 309
Cfr13I GGNCC 2 cut(s) 109, 165
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 300
CviAII CATG 3 cut(s) 106, 169, 235
CviJI RGCY 5 cut(s) 76, 167, 245, 295, 450
CviKI_1 RGCY 5 cut(s) 76, 167, 245, 295, 450
DdeI CTNAG 2 cut(s) 296, 446
DpnI GATC 3 cut(s) 6, 190, 261
DpnII GATC 3 cut(s) 4, 188, 259
DraI TTTAAA 1 cut(s) 91
Eam1104I CTCTTC 3 cut(s) 207, 222, 285
EarI CTCTTC 3 cut(s) 207, 222, 285
Eco130I CCWWGG 1 cut(s) 168
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 109
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
Esp3I CGTCTC 2 cut(s) 45, 153
FaeI CATG 3 cut(s) 109, 172, 238
FaiI YATR 4 cut(s) 107, 170, 236, 392
FaqI GGGAC 1 cut(s) 95
FatI CATG 3 cut(s) 105, 168, 234
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 1 cut(s) 441
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 1 cut(s) 441
GluI GCNGC 1 cut(s) 441
HaeIII GGCC 1 cut(s) 167
HgaI GACGC 1 cut(s) 300
Hin1II CATG 3 cut(s) 109, 172, 238
HinfI GANTC 1 cut(s) 102
HphI GGTGA 1 cut(s) 281
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 1 cut(s) 200
HpyCH4V TGCA 1 cut(s) 351
HpyF3I CTNAG 2 cut(s) 296, 446
Hsp92II CATG 3 cut(s) 109, 172, 238
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 3 cut(s) 4, 188, 259
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 1 cut(s) 126
Lsp1109I GCAGC 1 cut(s) 427
MalI GATC 3 cut(s) 6, 190, 261
MboI GATC 3 cut(s) 4, 188, 259
MboII GAAGA 7 cut(s) 70, 224, 236, 239, 302, 356, 367
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 3 cut(s) 14, 397, 454
MnlI CCTC 4 cut(s) 214, 223, 291, 419
MroXI GAANNNNTTC 1 cut(s) 83
MseI TTAA 4 cut(s) 90, 396, 402, 457
MvnI CGCG 1 cut(s) 311
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 4, 188, 259
NlaIII CATG 3 cut(s) 109, 172, 238
NlaIV GGNNCC 2 cut(s) 111, 166
PciSI GCTCTTC 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 442
PspN4I GGNNCC 2 cut(s) 111, 166
PspPI GGNCC 2 cut(s) 109, 165
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 4 cut(s) 90, 396, 402, 457
SatI GCNGC 1 cut(s) 441
Sau3AI GATC 3 cut(s) 4, 188, 259
Sau96I GGNCC 2 cut(s) 109, 165
SduI GDGCHC 1 cut(s) 309
SetI ASST 6 cut(s) 78, 297, 302, 372, 430, 452
SinI GGWCC 1 cut(s) 109
Sse9I AATT 3 cut(s) 14, 397, 454
StyI CCWWGG 1 cut(s) 168
TaqI TCGA 2 cut(s) 39, 177
TasI AATT 3 cut(s) 14, 397, 454
TfiI GAWTC 1 cut(s) 102
Tru1I TTAA 4 cut(s) 90, 396, 402, 457
Tru9I TTAA 4 cut(s) 90, 396, 402, 457
TseI GCWGC 1 cut(s) 440
TspDTI ATGAA 3 cut(s) 135, 268, 303
VpaK11BI GGWCC 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.