FvH4_2g17620

Calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012876
Physical Location & Seq
Reverse (-)
15145609 .. 15146079
471 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g17620.t1

Sequence Viewer

Length: 471 bp
ATGGACAGAGCAGAGCTCCGGAGGGTTTTCCAGATGTTCGACCGGAACGGGGACGGGCGCATCACCAAGCAGGAGCTGAGTGACTCATTGGAGAATCTAGGCATCTTCATCCCCGATAAGGACTTGCACCAGATGATAGAGAAGATCGACGCGAATGGAGATGGGTTTGTAGACATTGACGAGTTCGGGGCGTTGTATCAGAACCTGATGGACGAGAGGGACGACAGAGAGGACCCGGAGGAGGACATGAGGGAAGCGTTCAATGTGTTTGATCAGAACGGGGATGGTTTCATTACTGTGGAGGAGTTGAGATCGGTGTTGGCTTCCTTGGGGCTCAAGCAAGGGAGGACATTGGAGGATTGCAAGATTATGATAAAGAAGGTGGATGTGGATGGAGATGGGAGGGTGAATTTCAAGGAGTTTAAGCAGATGATGAAGGGGGGTGGATTTGCTGCCTTGACTTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko04626 map03018 map04626
Pfam Domains
Protein Families
CRS1_YhbY EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

157

Amino Acids

17.79

Weight (kDa)

4.45

Isoelectric Point (pI)

25.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 4 - 66 6e-14 EF-hand domain pair
EF-hand_1 PF00036 5 - 30 3.2e-07 EF hand domain
EF-hand_6 PF13405 5 - 34 5.7e-08 EF-hand domain
EF-hand_5 PF13202 6 - 27 3e-07 EF hand
EF-hand_1 PF00036 41 - 67 3.3e-06 EF hand domain
EF-hand_7 PF13499 80 - 146 3.8e-18 EF-hand domain pair
EF-hand_6 PF13405 82 - 111 1.3e-09 EF-hand domain
EF-hand_1 PF00036 83 - 110 1.2e-08 EF hand domain
EF-hand_5 PF13202 84 - 107 4.3e-08 EF hand
EF-hand_8 PF13833 98 - 146 3.9e-10 EF-hand domain pair
EF-hand_1 PF00036 121 - 146 3e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07490 AT4G12860
fragaria_vesca FvH4_2g17620
malus_domestica MD05G1156600.v1.1 MD10G1150400.v1.1
prunus_persica Prupe.8G188800_v2.0.a1
pyrus_communis pycom05g14920 pycom10g12960
rosa_chinensis RchiOBHm_Chr6g0282601
rosa_laevigata RLG00000012882
rosa_multiflora Rmu_co8242345.1_g000002 Rmu_sc0000283.1_g000002 Rmu_sc0005697.1_g000002
rosa_roxburghii Rroxscaffold_7G00186230
rosa_rugosa Rorug06G0148000
rosa_samantha Rh6BG264300 Rh6CG263400 Rh6DG255400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 171
AccII CGCG 1 cut(s) 152
AccIII TCCGGA 1 cut(s) 18
AcsI RAATTY 1 cut(s) 409
AfiI CCNNNNNNNGG 3 cut(s) 49, 118, 241
AgsI TTSAA 3 cut(s) 262, 415, 465
AluBI AGCT 2 cut(s) 16, 76
AluI AGCT 2 cut(s) 16, 76
Alw21I GWGCWC 1 cut(s) 18
AlwNI CAGNNNCTG 2 cut(s) 76, 205
Aor13HI TCCGGA 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 1 cut(s) 409
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 2 cut(s) 55, 418
AvaII GGWCC 1 cut(s) 232
BanII GRGCYC 2 cut(s) 18, 336
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 439
BccI CCATC 5 cut(s) 155, 202, 278, 386, 392
BclI TGATCA 1 cut(s) 271
BcnI CCSGG 1 cut(s) 236
BfaI CTAG 1 cut(s) 98
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 236
BmsI GCATC 2 cut(s) 69, 111
BplI GAGNNNNNCTC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 327
BsaWI WCCGGW 2 cut(s) 18, 42
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 118, 241
BseAI TCCGGA 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 4 cut(s) 108, 289, 391, 397
BseLI CCNNNNNNNGG 3 cut(s) 49, 118, 241
BseMII CTCAG 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 254, 317
BseXI GCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 43
BsiEI CGRYCG 1 cut(s) 43
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 3 cut(s) 19, 43, 236
BslFI GGGAC 2 cut(s) 65, 233
BslI CCNNNNNNNGG 3 cut(s) 49, 118, 241
BsmFI GGGAC 2 cut(s) 65, 233
Bsp1286I GDGCHC 2 cut(s) 18, 336
Bsp13I TCCGGA 1 cut(s) 18
Bsp143I GATC 3 cut(s) 144, 271, 311
BspCNI CTCAG 1 cut(s) 69
BspEI TCCGGA 1 cut(s) 18
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 3 cut(s) 144, 271, 311
BssT1I CCWWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 298
BstDEI CTNAG 1 cut(s) 77
BstF5I GGATG 4 cut(s) 108, 289, 391, 397
BstFNI CGCG 1 cut(s) 152
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 3 cut(s) 147, 274, 314
BstMBI GATC 3 cut(s) 144, 271, 311
BstMCI CGRYCG 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 234
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 439
BtsCI GGATG 4 cut(s) 108, 289, 391, 397
CaiI CAGNNNCTG 2 cut(s) 76, 205
CfoI GCGC 1 cut(s) 60
Cfr13I GGNCC 1 cut(s) 232
CseI GACGC 1 cut(s) 158
CviAII CATG 1 cut(s) 247
CviJI RGCY 4 cut(s) 16, 76, 323, 334
CviKI_1 RGCY 4 cut(s) 16, 76, 323, 334
DdeI CTNAG 1 cut(s) 77
DpnI GATC 3 cut(s) 146, 273, 313
DpnII GATC 3 cut(s) 144, 271, 311
Ecl136II GAGCTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 18, 336
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 18, 336
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 1 cut(s) 250
FaiI YATR 2 cut(s) 248, 371
FaqI GGGAC 2 cut(s) 65, 233
FatI CATG 1 cut(s) 246
FbaI TGATCA 1 cut(s) 271
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 4 cut(s) 95, 296, 398, 404
FriOI GRGCYC 2 cut(s) 18, 336
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 1 cut(s) 98
GlaI GCGC 1 cut(s) 59
GluI GCNGC 1 cut(s) 453
HapII CCGG 3 cut(s) 19, 43, 236
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 60
Hin1II CATG 1 cut(s) 250
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 2 cut(s) 83, 94
HpaII CCGG 3 cut(s) 19, 43, 236
HphI GGTGA 2 cut(s) 55, 418
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 2 cut(s) 201, 276
Hpy188III TCNNGA 2 cut(s) 19, 31
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 373, 430
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4V TGCA 2 cut(s) 127, 363
HpyF3I CTNAG 1 cut(s) 77
Hsp92II CATG 1 cut(s) 250
HspAI GCGC 1 cut(s) 58
Kpn2I TCCGGA 1 cut(s) 18
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 3 cut(s) 144, 271, 311
LmnI GCTCC 2 cut(s) 21, 73
LpnPI CCDG 7 cut(s) 32, 44, 56, 56, 143, 218, 249
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 2 cut(s) 69, 111
MaeI CTAG 1 cut(s) 98
MaeIII GTNAC 1 cut(s) 80
MalI GATC 3 cut(s) 146, 273, 313
MboI GATC 3 cut(s) 144, 271, 311
MboII GAAGA 2 cut(s) 97, 154
MhlI GDGCHC 2 cut(s) 18, 336
MluCI AATT 2 cut(s) 409, 466
MlyI GAGTC 1 cut(s) 77
MroI TCCGGA 1 cut(s) 18
MseI TTAA 2 cut(s) 423, 469
MslI CAYNNNNRTG 1 cut(s) 296
MspI CCGG 3 cut(s) 19, 43, 236
MspR9I CCNGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 152
NciI CCSGG 1 cut(s) 236
NdeII GATC 3 cut(s) 144, 271, 311
NlaIII CATG 1 cut(s) 250
NlaIV GGNNCC 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 1 cut(s) 94
PkrI GCNGC 1 cut(s) 454
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PpuMI RGGWCCY 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 232
PspPPI RGGWCCY 1 cut(s) 232
PstNI CAGNNNCTG 2 cut(s) 76, 205
RseI CAYNNNNRTG 1 cut(s) 296
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 423, 469
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 3 cut(s) 144, 271, 311
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 2 cut(s) 18, 336
SetI ASST 4 cut(s) 18, 78, 207, 384
SfaNI GCATC 2 cut(s) 69, 111
SinI GGWCC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 2 cut(s) 409, 466
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 298
TaqI TCGA 2 cut(s) 39, 147
TasI AATT 2 cut(s) 409, 466
TfiI GAWTC 1 cut(s) 94
Tru1I TTAA 2 cut(s) 423, 469
Tru9I TTAA 2 cut(s) 423, 469
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 3 cut(s) 97, 280, 449
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.