Rh6BG264300

Calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012876
Physical Location & Seq
Reverse (-)
47925140 .. 47925604
465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG264300.1

Sequence Viewer

Length: 465 bp
ATGGACAGAGCAGAGCTCCAACGGGTTTTCCAGATGTTCGACCGGAACGGGGACGGCCGCATCACCAAGCAGGAGCTGAGTCACTCTCTAGAGAATCTCGGCATCTTCATCCCCGACAAGGACTTGCACCAGATGATAGACAAGATCGACGCCAATGGAGATGGGTTTGTAGACATCGACGAGTTCGGGGCGTTGTACCAGAACCTGATGGACGAGAGGGACGAGGACCCGGAGGAGGACATGAGGGAAGCGTTCAATGTCTTTGATCAGAACGGCGATGGTTTCATCACCGTCGATGAGTTGAGGTCGGTGTTGGCTTCCTTGGGGCTCAAGCAAGGGAGGACGTTGGAGGATTGCAAGATTATGATAAAGAAGGTGGATGTGGATGGAGATGGGAGGGTGAATTTCAAGGAGTTTAAGCAGATGATGAAGGGTGGTGGCTTTGCTGCTTTGAGTTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko04626 map03018 map04626
Pfam Domains
Protein Families
CRS1_YhbY EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

154

Amino Acids

17.47

Weight (kDa)

4.45

Isoelectric Point (pI)

16.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 4 - 66 4.3e-14 EF-hand domain pair
EF-hand_1 PF00036 5 - 30 3.4e-07 EF hand domain
EF-hand_6 PF13405 5 - 34 6.5e-08 EF-hand domain
EF-hand_5 PF13202 6 - 27 2.7e-07 EF hand
EF_EFCAB10_C PF24548 6 - 50 6.1e-06 EFCAB10, C-terminal EF-hand
EF-hand_1 PF00036 41 - 67 4.5e-06 EF hand domain
EF-hand_7 PF13499 78 - 144 6.4e-18 EF-hand domain pair
EF-hand_6 PF13405 80 - 109 1.1e-09 EF-hand domain
EF-hand_1 PF00036 81 - 108 1.5e-08 EF hand domain
EF-hand_5 PF13202 82 - 105 9.7e-08 EF hand
EF-hand_8 PF13833 96 - 144 5.6e-10 EF-hand domain pair
EF-hand_1 PF00036 119 - 144 2.9e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07490 AT4G12860
fragaria_vesca FvH4_2g17620
malus_domestica MD05G1156600.v1.1 MD10G1150400.v1.1
prunus_persica Prupe.8G188800_v2.0.a1
pyrus_communis pycom05g14920 pycom10g12960
rosa_chinensis RchiOBHm_Chr6g0282601
rosa_laevigata RLG00000012882
rosa_multiflora Rmu_co8242345.1_g000002 Rmu_sc0000283.1_g000002 Rmu_sc0005697.1_g000002
rosa_roxburghii Rroxscaffold_7G00186230
rosa_rugosa Rorug06G0148000
rosa_samantha Rh6BG264300 Rh6CG263400 Rh6DG255400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 58
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 403
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 3 cut(s) 49, 118, 235
AgsI TTSAA 3 cut(s) 256, 409, 459
AluBI AGCT 2 cut(s) 16, 76
AluI AGCT 2 cut(s) 16, 76
Alw21I GWGCWC 1 cut(s) 18
AlwNI CAGNNNCTG 2 cut(s) 76, 205
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 403
AspS9I GGNCC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 3 cut(s) 55, 280, 412
AvaII GGWCC 1 cut(s) 226
BanII GRGCYC 2 cut(s) 18, 330
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 433
BccI CCATC 5 cut(s) 155, 202, 272, 380, 386
BceAI ACGGC 2 cut(s) 70, 289
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 1 cut(s) 230
BfaI CTAG 1 cut(s) 89
BisI GCNGC 2 cut(s) 58, 447
BlsI GCNGC 2 cut(s) 59, 448
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 2 cut(s) 69, 111
BplI GAGNNNNNCTC 3 cut(s) 32, 70, 102
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 230
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 321
BsaWI WCCGGW 1 cut(s) 42
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 118, 235
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 3 cut(s) 108, 385, 391
BseLI CCNNNNNNNGG 3 cut(s) 49, 118, 235
BseMII CTCAG 1 cut(s) 68
BseRI GAGGAG 1 cut(s) 248
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 433
Bsh1285I CGRYCG 2 cut(s) 43, 58
BshFI GGCC 1 cut(s) 57
BsiEI CGRYCG 2 cut(s) 43, 58
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 2 cut(s) 43, 230
BslFI GGGAC 2 cut(s) 65, 233
BslI CCNNNNNNNGG 3 cut(s) 49, 118, 235
BsmFI GGGAC 2 cut(s) 65, 233
BsnI GGCC 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 18, 330
Bsp143I GATC 2 cut(s) 144, 265
BspACI CCGC 1 cut(s) 58
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 228
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 2 cut(s) 144, 265
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 292
BstACI GRCGYC 1 cut(s) 150
BstDEI CTNAG 1 cut(s) 77
BstF5I GGATG 3 cut(s) 108, 385, 391
BstKTI GATC 2 cut(s) 147, 268
BstMBI GATC 2 cut(s) 144, 265
BstMCI CGRYCG 2 cut(s) 43, 58
BstSCI CCNGG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 433
BstZI CGGCCG 1 cut(s) 55
BsuRI GGCC 1 cut(s) 57
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 3 cut(s) 108, 385, 391
CaiI CAGNNNCTG 2 cut(s) 76, 205
Cfr13I GGNCC 1 cut(s) 226
CseI GACGC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 196
CviAII CATG 1 cut(s) 241
CviJI RGCY 6 cut(s) 16, 57, 76, 317, 328, 441
CviKI_1 RGCY 6 cut(s) 16, 57, 76, 317, 328, 441
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 77
DpnI GATC 2 cut(s) 146, 267
DpnII GATC 2 cut(s) 144, 265
EaeI YGGCCR 1 cut(s) 55
EagI CGGCCG 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 16
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 321
Eco24I GRGCYC 2 cut(s) 18, 330
Eco47I GGWCC 1 cut(s) 226
Eco52I CGGCCG 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 321
EcoT38I GRGCYC 2 cut(s) 18, 330
ErhI CCWWGG 1 cut(s) 321
FaeI CATG 1 cut(s) 244
FaiI YATR 2 cut(s) 242, 365
FaqI GGGAC 2 cut(s) 65, 233
FatI CATG 1 cut(s) 240
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 58, 447
FokI GGATG 3 cut(s) 95, 392, 398
FriOI GRGCYC 2 cut(s) 18, 330
Fsp4HI GCNGC 2 cut(s) 58, 447
FspBI CTAG 1 cut(s) 89
GluI GCNGC 2 cut(s) 58, 447
HaeIII GGCC 1 cut(s) 57
HapII CCGG 2 cut(s) 43, 230
HgaI GACGC 1 cut(s) 158
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 244
HinfI GANTC 2 cut(s) 79, 94
HpaII CCGG 2 cut(s) 43, 230
HphI GGTGA 3 cut(s) 55, 280, 412
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 2 cut(s) 31, 89
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 3 cut(s) 152, 182, 296
HpyAV CCTTC 2 cut(s) 367, 424
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 2 cut(s) 127, 357
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 1 cut(s) 344
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 2 cut(s) 144, 265
LmnI GCTCC 2 cut(s) 21, 73
LpnPI CCDG 7 cut(s) 44, 56, 56, 143, 212, 218, 243
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 2 cut(s) 69, 111
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 80
MalI GATC 2 cut(s) 146, 267
MboI GATC 2 cut(s) 144, 265
MboII GAAGA 1 cut(s) 97
MhlI GDGCHC 2 cut(s) 18, 330
MluCI AATT 2 cut(s) 403, 460
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 2 cut(s) 43, 327
MnlI CCTC 9 cut(s) 210, 217, 226, 229, 237, 297, 333, 343, 390
MseI TTAA 2 cut(s) 417, 463
MspI CCGG 2 cut(s) 43, 230
MspR9I CCNGG 1 cut(s) 230
NciI CCSGG 1 cut(s) 230
NdeII GATC 2 cut(s) 144, 265
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 1 cut(s) 228
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 1 cut(s) 94
PkrI GCNGC 2 cut(s) 59, 448
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PpuMI RGGWCCY 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 226
PspPPI RGGWCCY 1 cut(s) 226
PstNI CAGNNNCTG 2 cut(s) 76, 205
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 417, 463
SatI GCNGC 2 cut(s) 58, 447
Sau3AI GATC 2 cut(s) 144, 265
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 2 cut(s) 18, 330
SetI ASST 6 cut(s) 18, 78, 207, 308, 347, 378
SfaNI GCATC 2 cut(s) 69, 111
SinI GGWCC 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 2 cut(s) 403, 460
SsiI CCGC 1 cut(s) 58
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 1 cut(s) 321
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 347
TaqI TCGA 4 cut(s) 39, 147, 177, 294
TasI AATT 2 cut(s) 403, 460
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 1 cut(s) 94
Tru1I TTAA 2 cut(s) 417, 463
Tru9I TTAA 2 cut(s) 417, 463
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 3 cut(s) 97, 274, 443
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 1 cut(s) 403
XbaI TCTAGA 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.