Rroxscaffold_7G00186230

Calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012876
Physical Location & Seq
Forward (+)
25603280 .. 25603711
432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00186230.1

Sequence Viewer

Length: 432 bp
ATGTTCGACCGGAACGGCGACGGCCGCATCACCAAGCAGGAGCTGAGTCACTCGCTAGAGAATCTCGGCATCTTCATCCCCGACAAGGACTTGCACCAGATGATAGACAAGATCGACGCCAATGGAGATGGGTTTGTAGACATCGACGAGTTCGGGGCGTTGTACCAGAACCTGATGGACGAGAGGGACGAGGACCCGGAGGAGGACATGAGGGAAGCGTTCAATGTGTTTGATCAGAACGGCGATGGTTTCATCACCGTCGATGAGTTGAGGTCGGTGTTGGCTTCCTTGGGGCTCAAGCAAGGGAGGACGTTGGAGGATTGCAAGATTATGATAAAGAAGGTGGATGTGGATGGAGATGGCAGGGTGAATTTCAAGGAGTTTAAGCAGATGATGAAGGGTGGTGGCTTTGCTGCTTTGAGTTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko04626 map03018 map04626
Pfam Domains
Protein Families
CRS1_YhbY EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

143

Amino Acids

16.1

Weight (kDa)

4.4

Isoelectric Point (pI)

15.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 1 - 55 5e-10 EF-hand domain pair
EF-hand_1 PF00036 30 - 56 4e-06 EF hand domain
EF-hand_7 PF13499 67 - 133 5.4e-18 EF-hand domain pair
EF-hand_6 PF13405 69 - 98 9.9e-10 EF-hand domain
EF-hand_1 PF00036 70 - 97 1.3e-08 EF hand domain
EF-hand_5 PF13202 71 - 94 8.6e-08 EF hand
EF-hand_8 PF13833 85 - 133 4.8e-10 EF-hand domain pair
EF-hand_1 PF00036 108 - 133 2.6e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07490 AT4G12860
fragaria_vesca FvH4_2g17620
malus_domestica MD05G1156600.v1.1 MD10G1150400.v1.1
prunus_persica Prupe.8G188800_v2.0.a1
pyrus_communis pycom05g14920 pycom10g12960
rosa_chinensis RchiOBHm_Chr6g0282601
rosa_laevigata RLG00000012882
rosa_multiflora Rmu_co8242345.1_g000002 Rmu_sc0000283.1_g000002 Rmu_sc0005697.1_g000002
rosa_roxburghii Rroxscaffold_7G00186230
rosa_rugosa Rorug06G0148000
rosa_samantha Rh6BG264300 Rh6CG263400 Rh6DG255400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 138
AciI CCGC 1 cut(s) 25
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 1 cut(s) 370
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 85, 202
AgsI TTSAA 3 cut(s) 223, 376, 426
AluBI AGCT 1 cut(s) 43
AluI AGCT 1 cut(s) 43
AlwNI CAGNNNCTG 2 cut(s) 43, 172
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 370
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 3 cut(s) 22, 247, 379
AvaII GGWCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 400
BccI CCATC 5 cut(s) 122, 169, 239, 347, 353
BceAI ACGGC 3 cut(s) 31, 37, 256
BclI TGATCA 1 cut(s) 232
BcnI CCSGG 1 cut(s) 197
BfaI CTAG 1 cut(s) 56
BisI GCNGC 2 cut(s) 25, 414
BlsI GCNGC 2 cut(s) 26, 415
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 197
BmsI GCATC 2 cut(s) 36, 78
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 202
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 3 cut(s) 75, 352, 358
BseLI CCNNNNNNNGG 2 cut(s) 85, 202
BseMII CTCAG 1 cut(s) 35
BseRI GAGGAG 1 cut(s) 215
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 400
Bsh1285I CGRYCG 2 cut(s) 10, 25
BshFI GGCC 1 cut(s) 24
BsiEI CGRYCG 2 cut(s) 10, 25
BsiSI CCGG 2 cut(s) 10, 197
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 85, 202
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 1 cut(s) 24
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 2 cut(s) 111, 232
BspACI CCGC 1 cut(s) 25
BspANI GGCC 1 cut(s) 24
BspCNI CTCAG 1 cut(s) 36
BspLI GGNNCC 1 cut(s) 195
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 2 cut(s) 111, 232
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 259
BstACI GRCGYC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 44
BstF5I GGATG 3 cut(s) 75, 352, 358
BstKTI GATC 2 cut(s) 114, 235
BstMBI GATC 2 cut(s) 111, 232
BstMCI CGRYCG 2 cut(s) 10, 25
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 400
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 258
BtsCI GGATG 3 cut(s) 75, 352, 358
CaiI CAGNNNCTG 2 cut(s) 43, 172
Cfr13I GGNCC 1 cut(s) 193
CseI GACGC 1 cut(s) 125
Csp6I GTAC 1 cut(s) 163
CviAII CATG 1 cut(s) 208
CviJI RGCY 5 cut(s) 24, 43, 284, 295, 408
CviKI_1 RGCY 5 cut(s) 24, 43, 284, 295, 408
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 1 cut(s) 44
DpnI GATC 2 cut(s) 113, 234
DpnII GATC 2 cut(s) 111, 232
EaeI YGGCCR 1 cut(s) 22
EagI CGGCCG 1 cut(s) 22
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 193
Eco52I CGGCCG 1 cut(s) 22
EcoO109I RGGNCCY 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 1 cut(s) 211
FaiI YATR 2 cut(s) 209, 332
FaqI GGGAC 1 cut(s) 200
FatI CATG 1 cut(s) 207
FbaI TGATCA 1 cut(s) 232
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 2 cut(s) 25, 414
FokI GGATG 3 cut(s) 62, 359, 365
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 25, 414
FspBI CTAG 1 cut(s) 56
GluI GCNGC 2 cut(s) 25, 414
HaeIII GGCC 1 cut(s) 24
HapII CCGG 2 cut(s) 10, 197
HgaI GACGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 1 cut(s) 211
HinfI GANTC 2 cut(s) 46, 61
HpaII CCGG 2 cut(s) 10, 197
HphI GGTGA 3 cut(s) 22, 247, 379
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 1 cut(s) 237
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 4 cut(s) 23, 119, 149, 263
HpyAV CCTTC 2 cut(s) 334, 391
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 2 cut(s) 94, 324
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 2 cut(s) 111, 232
LmnI GCTCC 1 cut(s) 40
LpnPI CCDG 7 cut(s) 23, 23, 110, 179, 185, 210, 349
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 2 cut(s) 36, 78
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 47
MalI GATC 2 cut(s) 113, 234
MboI GATC 2 cut(s) 111, 232
MboII GAAGA 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 2 cut(s) 370, 427
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 8 cut(s) 177, 184, 193, 196, 204, 264, 300, 310
MseI TTAA 2 cut(s) 384, 430
MspI CCGG 2 cut(s) 10, 197
MspR9I CCNGG 1 cut(s) 197
MwoI GCNNNNNNNGC 1 cut(s) 24
NciI CCSGG 1 cut(s) 197
NdeII GATC 2 cut(s) 111, 232
NlaIII CATG 1 cut(s) 211
NlaIV GGNNCC 1 cut(s) 195
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PfeI GAWTC 1 cut(s) 61
PkrI GCNGC 2 cut(s) 26, 415
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PpuMI RGGWCCY 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 1 cut(s) 193
PspPPI RGGWCCY 1 cut(s) 193
PstNI CAGNNNCTG 2 cut(s) 43, 172
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SaqAI TTAA 2 cut(s) 384, 430
SatI GCNGC 2 cut(s) 25, 414
Sau3AI GATC 2 cut(s) 111, 232
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 1 cut(s) 297
SetI ASST 5 cut(s) 45, 174, 275, 314, 345
SfaNI GCATC 2 cut(s) 36, 78
SinI GGWCC 1 cut(s) 193
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 2 cut(s) 370, 427
SsiI CCGC 1 cut(s) 25
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 1 cut(s) 314
TaqI TCGA 4 cut(s) 6, 114, 144, 261
TasI AATT 2 cut(s) 370, 427
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 1 cut(s) 61
Tru1I TTAA 2 cut(s) 384, 430
Tru9I TTAA 2 cut(s) 384, 430
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 3 cut(s) 64, 241, 410
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.