pycom10g12960

CRM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012876
Physical Location & Seq
Reverse (-)
16425311 .. 16425769
459 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12960.1

Sequence Viewer

Length: 459 bp
ATGGACAAGGCGGAGCTACGGCGGGTTTTCCATATGTTTGATCAGAACGGGGACGGGCGGATCACGAAGAAGGAGATGAGGGACTCGTTGGAGAAGCTGGGGATTTACATTCCGGACAAGGACTTGATCCAGATGATCGAAAAAATCGATGTGAATGGAGATGGGTTCGTGGACATGGATGAGTTCGGGGCGTTGTATCAGACGATCATGGACGAGAGGGATGAGGAGGAGGACATGAGGGAGGCGTTCAATGTGTTTGATCAGAATGGAGACGGGTTCATCACGGTGGACGAGCTGCGGTCGGTTTTGTCTTCCTTGGGGCTCAAGCAAGGGAAGACATTGGAGGATTGTACGATCATGATAAAGAAGGTTGATGTGGATGGTGATGGGAGGGTGAATTTCAAGGAGTTCAAGCAGATGATGAAAGGTGGTGGGTTTGCTGCCTTAAGTTCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CRS1_YhbY EF-hand_1 EF-hand_5

Protein Analysis

153

Amino Acids

17.32

Weight (kDa)

4.53

Isoelectric Point (pI)

23.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 4 - 66 2.1e-14 EF-hand domain pair
EF-hand_1 PF00036 5 - 32 6.6e-07 EF hand domain
EF-hand_6 PF13405 5 - 34 3.3e-08 EF-hand domain
EF-hand_5 PF13202 6 - 27 6.5e-06 EF hand
EF-hand_7 PF13499 76 - 142 5.9e-18 EF-hand domain pair
EF-hand_6 PF13405 78 - 107 8.1e-10 EF-hand domain
EF-hand_1 PF00036 79 - 106 1.4e-08 EF hand domain
EF-hand_5 PF13202 80 - 103 9.5e-08 EF hand
EF-hand_8 PF13833 94 - 142 3.2e-10 EF-hand domain pair
EF-hand_1 PF00036 120 - 142 7.7e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012876)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07490 AT4G12860
fragaria_vesca FvH4_2g17620
malus_domestica MD05G1156600.v1.1 MD10G1150400.v1.1
prunus_persica Prupe.8G188800_v2.0.a1
pyrus_communis pycom05g14920 pycom10g12960
rosa_chinensis RchiOBHm_Chr6g0282601
rosa_laevigata RLG00000012882
rosa_multiflora Rmu_co8242345.1_g000002 Rmu_sc0000283.1_g000002 Rmu_sc0005697.1_g000002
rosa_roxburghii Rroxscaffold_7G00186230
rosa_rugosa Rorug06G0148000
rosa_samantha Rh6BG264300 Rh6CG263400 Rh6DG255400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 112
AciI CCGC 4 cut(s) 11, 22, 58, 298
AclWI GGATC 2 cut(s) 68, 121
AcsI RAATTY 1 cut(s) 397
AfaI GTAC 1 cut(s) 352
AflII CTTAAG 1 cut(s) 445
AgsI TTSAA 4 cut(s) 250, 403, 412, 453
AluBI AGCT 3 cut(s) 16, 97, 295
AluI AGCT 3 cut(s) 16, 97, 295
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 2 cut(s) 68, 121
Aor13HI TCCGGA 1 cut(s) 112
ApeKI GCWGC 2 cut(s) 295, 440
ApoI RAATTY 1 cut(s) 397
AsuHPI GGTGA 2 cut(s) 395, 406
BanII GRGCYC 1 cut(s) 324
BbsI GAAGAC 2 cut(s) 303, 341
BbvI GCAGC 2 cut(s) 282, 427
BccI CCATC 3 cut(s) 155, 374, 380
BceAI ACGGC 1 cut(s) 35
BclI TGATCA 2 cut(s) 40, 259
BcoDI GTCTC 1 cut(s) 264
BfrI CTTAAG 1 cut(s) 445
BisI GCNGC 2 cut(s) 296, 441
BlsI GCNGC 2 cut(s) 297, 442
BpiI GAAGAC 2 cut(s) 303, 341
BpuEI CTTGAG 1 cut(s) 308
Bsa29I ATCGAT 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 112
BseAI TCCGGA 1 cut(s) 112
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 3 cut(s) 184, 226, 385
BseRI GAGGAG 2 cut(s) 239, 242
BseXI GCAGC 2 cut(s) 282, 427
BseYI CCCAGC 1 cut(s) 97
Bsh1285I CGRYCG 1 cut(s) 302
BshVI ATCGAT 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 302
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 2 cut(s) 65, 95
BsmAI GTCTC 1 cut(s) 264
BsmBI CGTCTC 1 cut(s) 264
BsmFI GGGAC 2 cut(s) 65, 95
Bsp1286I GDGCHC 1 cut(s) 324
Bsp13I TCCGGA 1 cut(s) 112
Bsp143I GATC 7 cut(s) 40, 60, 126, 135, 204, 259, 354
BspACI CCGC 4 cut(s) 11, 22, 58, 298
BspDI ATCGAT 1 cut(s) 147
BspEI TCCGGA 1 cut(s) 112
BspHI TCATGA 1 cut(s) 357
BspPI GGATC 2 cut(s) 68, 121
BspTI CTTAAG 1 cut(s) 445
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 7 cut(s) 40, 60, 126, 135, 204, 259, 354
BssT1I CCWWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 286
BstAFI CTTAAG 1 cut(s) 445
BstF5I GGATG 3 cut(s) 184, 226, 385
BstKTI GATC 7 cut(s) 43, 63, 129, 138, 207, 262, 357
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 7 cut(s) 40, 60, 126, 135, 204, 259, 354
BstMCI CGRYCG 1 cut(s) 302
BstV1I GCAGC 2 cut(s) 282, 427
BstV2I GAAGAC 2 cut(s) 303, 341
Bsu15I ATCGAT 1 cut(s) 147
BsuTUI ATCGAT 1 cut(s) 147
BtsCI GGATG 3 cut(s) 184, 226, 385
CciI TCATGA 1 cut(s) 357
ClaI ATCGAT 1 cut(s) 147
Csp6I GTAC 1 cut(s) 351
CviAII CATG 4 cut(s) 175, 208, 235, 358
CviJI RGCY 4 cut(s) 16, 97, 295, 322
CviKI_1 RGCY 4 cut(s) 16, 97, 295, 322
CviQI GTAC 1 cut(s) 351
DpnI GATC 7 cut(s) 42, 62, 128, 137, 206, 261, 356
DpnII GATC 7 cut(s) 40, 60, 126, 135, 204, 259, 354
EciI GGCGGA 2 cut(s) 26, 73
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 4 cut(s) 178, 211, 238, 361
FaiI YATR 6 cut(s) 33, 35, 176, 209, 236, 359
FaqI GGGAC 2 cut(s) 65, 95
FatI CATG 4 cut(s) 174, 207, 234, 357
FauI CCCGC 1 cut(s) 15
FauNDI CATATG 1 cut(s) 33
FbaI TGATCA 2 cut(s) 40, 259
Fnu4HI GCNGC 2 cut(s) 296, 441
FokI GGATG 3 cut(s) 191, 233, 392
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 2 cut(s) 296, 441
GluI GCNGC 2 cut(s) 296, 441
GsaI CCCAGC 1 cut(s) 101
HapII CCGG 1 cut(s) 113
Hin1II CATG 4 cut(s) 178, 211, 238, 361
HinfI GANTC 1 cut(s) 83
HpaII CCGG 1 cut(s) 113
HphI GGTGA 2 cut(s) 395, 406
Hpy166II GTNNAC 2 cut(s) 172, 289
Hpy188I TCNGA 3 cut(s) 45, 201, 264
Hpy188III TCNNGA 4 cut(s) 64, 113, 130, 358
Hpy8I GTNNAC 2 cut(s) 172, 289
HpyAV CCTTC 2 cut(s) 64, 361
HpyCH4III ACNGT 1 cut(s) 286
Hsp92II CATG 4 cut(s) 178, 211, 238, 361
Kpn2I TCCGGA 1 cut(s) 112
Ksp22I TGATCA 2 cut(s) 40, 259
Kzo9I GATC 7 cut(s) 40, 60, 126, 135, 204, 259, 354
LmnI GCTCC 1 cut(s) 13
LpnPI CCDG 3 cut(s) 83, 126, 143
Lsp1109I GCAGC 2 cut(s) 282, 427
MalI GATC 7 cut(s) 42, 62, 128, 137, 206, 261, 356
MboI GATC 7 cut(s) 40, 60, 126, 135, 204, 259, 354
MboII GAAGA 3 cut(s) 79, 303, 346
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 2 cut(s) 397, 454
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 9 cut(s) 72, 210, 217, 220, 223, 231, 235, 337, 384
MroI TCCGGA 1 cut(s) 112
MseI TTAA 1 cut(s) 446
MslI CAYNNNNRTG 1 cut(s) 284
MspCI CTTAAG 1 cut(s) 445
MspI CCGG 1 cut(s) 113
NdeI CATATG 1 cut(s) 33
NdeII GATC 7 cut(s) 40, 60, 126, 135, 204, 259, 354
NlaIII CATG 4 cut(s) 178, 211, 238, 361
PagI TCATGA 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PkrI GCNGC 2 cut(s) 297, 442
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PspFI CCCAGC 1 cut(s) 97
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 2 cut(s) 296, 441
Sau3AI GATC 7 cut(s) 40, 60, 126, 135, 204, 259, 354
SchI GAGTC 1 cut(s) 77
SduI GDGCHC 1 cut(s) 324
SetI ASST 5 cut(s) 18, 99, 297, 372, 430
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 323, 445
SmoI CTYRAG 2 cut(s) 323, 445
Sse9I AATT 2 cut(s) 397, 454
SsiI CCGC 4 cut(s) 11, 22, 58, 298
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 2 cut(s) 138, 147
TasI AATT 2 cut(s) 397, 454
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TseI GCWGC 2 cut(s) 295, 440
TspDTI ATGAA 2 cut(s) 268, 437
Vha464I CTTAAG 1 cut(s) 445
XapI RAATTY 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.